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1. 发明专利

AU6403694A Animal 2-5a-dependent rnases and encoding sequences therefor 未知
公开(公告)号：AU6403694A
公开(公告)日：1994-09-26
申请号：AU6403694
申请日：1994-03-03
申请人： CLEVELAND CLINIC FOUNDATION
发明人： SILVERMAN ROBERT H , HASSEL BRET A , ZHOU AIMIN
IPC分类号： C12N9/22 , C12N15/55 , C12N1/21 , C12N5/10 , C12N15/63
摘要： Isolated 2-5A-dependent RNases, an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells, and encoding sequences therefor are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases is also disclosed. Recombinant human 2-5A-dependent RNase produced in vitro bound an activating affinity matrix, 2-5A-cellulose, resulting in ribonuclease activity. The 2-5A binding properties of the recombinant and naturally occurring forms of 2-5A-dependent RNase are basically identical. Interferon induction of 2-5A-dependent RNase expression is demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNase sequences revealed several features, including similarity to RNase E which is implicated in the control of mRNA stability in E. coli. A duplicated phosphate-binding loop motif is determined by deletion analysis and site-directed mutagenesis to function in the binding of 2-5A.

2. 发明专利

BR9404382A 未知
公开(公告)号：BR9404382A
公开(公告)日：1999-06-15
申请号：BR9404382
申请日：1994-03-03
申请人： CLEVELAND CLINIC FOUNDATION
发明人： SILVERMAN ROBERT H , HASSEL BRET A , ZHOU AIMIN
IPC分类号： C12N9/22 , C12N15/55 , C12N1/21 , C12N5/10
摘要： Isolated 2-5A-dependent RNases, an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells, and encoding sequences therefor are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases is also disclosed. Recombinant human 2-5A-dependent RNase produced in vitro bound an activating affinity matrix, 2-5A-cellulose, resulting in ribonuclease activity. The 2-5A binding properties of the recombinant and naturally occurring forms of 2-5A-dependent RNase are basically identical. Interferon induction of 2-5A-dependent RNase expression is demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNase sequences revealed several features, including similarity to RNase E which is implicated in the control of mRNA stability in E. coli. A duplicated phosphate-binding loop motif is determined by deletion analysis and site-directed mutagenesis to function in the binding of 2-5A.

3. 发明专利

CA2135307A1 ANIMAL 2-5A-DEPENDENT RNASES AND ENCODING SEQUENCES THEREFOR 未知
公开(公告)号：CA2135307A1
公开(公告)日：1994-09-15
申请号：CA2135307
申请日：1994-03-03
申请人： CLEVELAND CLINIC FOUNDATION
发明人： SILVERMAN ROBERT H , HASSEL BRET A , ZHOU AIMIN
IPC分类号： C12N9/22 , C12N15/55
摘要： 2135307 9420605 PCTABS00033 Isolated 2-5A-dependent RNases, an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells (see Figs. 1 and 11), and encoding sequences therefor (see Figs. 3, 4, 9 and 10) are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases are also disclosed. Recombinant human 2-5A-dependent RNase produced in vitro and bound an activating affinity matrix, 2-5A-cellulose, have ribonuclease activity. The 2-5A binding properties of the recombinant and naturally occuring forms of 2-5A-dependent RNase are basically identical. Interferon induction of 2-5A-dependent RNase expression is demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNase sequences revealed several features, including similarity to RNase E which is implicated in the control of mRNA stability in E. coli.

4. 发明公开

EP0658193A4 ANIMAL 2-5A-DEPENDENT RNases AND ENCODING SEQUENCES THEREFOR. 失效
标题翻译：从动物Z-5A依赖核糖核酸酶和您的编码序列。
公开(公告)号：EP0658193A4
公开(公告)日：1998-07-29
申请号：EP94911455
申请日：1994-03-03
申请人： CLEVELAND CLINIC FOUNDATION
发明人： SILVERMAN ROBERT H , HASSEL BRET A , ZHOU AIMIN
IPC分类号： C12N9/22 , C12N15/55 , C12N1/21 , C12N5/10 , C12N15/63
CPC分类号： C12N9/22
摘要： Isolated 2-5A-dependent RNases, an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells (see Figs. 1 and 11), and encoding sequences therefor (see Figs. 3, 4, 9 and 10) are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases are also disclosed. Recombinant human 2-5A-dependent RNase produced in vitro and bound an activating affinity matrix, 2-5A-cellulose, have ribonuclease activity. The 2-5A binding properties of the recombinant and naturally occuring forms of 2-5A-dependent RNase are basically identical. Interferon induction of 2-5A-dependent RNase expression is demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNase sequences revealed several features, including similarity to RNase E which is implicated in the control of mRNA stability in E. coli.

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