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1. 发明专利

AU2003302462A8 Bead-based detection of ligand-gpcr-g protein complexes 未知
公开(公告)号：AU2003302462A8
公开(公告)日：2004-06-18
申请号：AU2003302462
申请日：2003-05-05
申请人： PROSSNITZ ERIC R , KEY ALEX T , SHI MEI , BURANDA TIONE , SKLAR LARRY A , NEUBIG RICHARD , SIMONS PETER C , CIMINO DANIEL , SCIENCE AND TECHNOLOGY CORP UN
发明人： KEY ALEX T , BURANDA TIONE , CIMINO DANIEL , SKLAR LARRY A , PROSSNITZ ERIC R , NEUBIG RICHARD , SHI MEI , SIMONS PETER C
IPC分类号： B01F13/00 , B01F15/02 , C07D233/76 , C07D401/12 , C07D405/12 , C12N5/02 , C12P21/04 , C12Q1/00 , C12P27/00 , C07K17/00 , C07K7/00 , G01N33/53 , C12Q1/68
摘要： The present invention provides a compound and a method of making a compound according to the following formula: where R 1 is an alkyl group having 1 to 12 carbons; R 2 is a urea group; R 3 is an active group, such as fluorescein, a toxin, radiolabel or drug; and R 4 , R 5 and R 6 are each independently a halide group.

2. 发明专利

AU2003302462A1 BEAD-BASED DETECTION OF LIGAND-GPCR-G PROTEIN COMPLEXES 未知
公开(公告)号：AU2003302462A1
公开(公告)日：2004-06-18
申请号：AU2003302462
申请日：2003-05-05
申请人： CIMINO DANIEL , BURANDA TIONE , KEY ALEX T , NEUBIG RICHARD , SCIENCE AND TECHNOLOGY CORP UN , SKLAR LARRY A , PROSSNITZ ERIC R , SIMONS PETER C , SHI MEI
发明人： BURANDA TIONE , KEY ALEX T , NEUBIG RICHARD , SKLAR LARRY A , PROSSNITZ ERIC R , SIMONS PETER C , SHI MEI , CIMINO DANIEL
IPC分类号： B01F13/00 , B01F15/02 , C07D233/76 , C07D401/12 , C07D405/12 , C12N5/02 , C12P21/04
摘要： The present invention provides a compound and a method of making a compound according to the following formula: where R 1 is an alkyl group having 1 to 12 carbons; R 2 is a urea group; R 3 is an active group, such as fluorescein, a toxin, radiolabel or drug; and R 4 , R 5 and R 6 are each independently a halide group.

3. 发明申请

WO2004048922A2 BEAD-BASED DETECTION OF LIGAND-GPCR-G PROTEIN COMPLEXES 审中-公开
标题翻译：基于珠粒检测的LIGAND-GPCR-G蛋白复合物
公开(公告)号：WO2004048922A2
公开(公告)日：2004-06-10
申请号：PCT/US0313851
申请日：2003-05-05
申请人： SCIENCE AND TECHNOLOGY CORP UN , SKLAR LARRY A , PROSSNITZ ERIC R , SIMONS PETER C , SHI MEI , CIMINO DANIEL , BURANDA TIONE , KEY ALEX T , NEUBIG RICHARD
发明人： SKLAR LARRY A , PROSSNITZ ERIC R , SIMONS PETER C , SHI MEI , CIMINO DANIEL , BURANDA TIONE , KEY ALEX T , NEUBIG RICHARD
IPC分类号： B01F13/00 , B01F15/02 , C07D233/76 , C07D401/12 , C07D405/12 , C12N5/02 , C12P21/04 , G01N
CPC分类号： C07D401/12 , B01F13/0059 , B01F13/0093 , B01F13/0096 , B01F15/0201 , B01F15/024 , C07D233/76 , C07D405/12
摘要： The present invention provides a general approach for G protein coupled receptors that may be used to define agonists and antagonists, and the specificity of receptor coupling to G protein subunits. Methods of the present invention use small volumes (microliters) and are compatible with high throughput flow cytometry. When assays of the present invention are multiplexed, the specificity of the interactions of a receptor with many G proteins may be determined simultaneously.
摘要翻译：本发明提供了可用于定义激动剂和拮抗剂的G蛋白偶联受体的一般方法以及受体与G蛋白亚基偶联的特异性。本发明的方法使用小体积（微升）并且与高通量流式细胞术兼容。当本发明的测定被复用时，可以同时测定受体与许多G蛋白的相互作用的特异性。

4. 发明申请

WO0239083A3 FLUORESCENCE AND FRET BASED ASSAYS FOR BIOMOLECULES ON BEADS 审中-公开
标题翻译：用于生物分子的荧光和基于FRET的测定
公开(公告)号：WO0239083A3
公开(公告)日：2002-07-11
申请号：PCT/US0142983
申请日：2001-11-06
申请人： STC UNM , BURANDA TIONE , HUANG JINMAN , PEREZ-LUNA VICTOR H , LOPEZ GABRIEL P , SIMONS PETER , SKLAR LARRY A
发明人： BURANDA TIONE , HUANG JINMAN , PEREZ-LUNA VICTOR H , LOPEZ GABRIEL P , SIMONS PETER , SKLAR LARRY A
IPC分类号： G01N21/64 , G01N33/542 , G01N33/543 , C07K16/00 , C12M1/34 , C12M3/00 , C12Q1/00 , G01N21/00 , G01N33/53 , G01N33/544 , G01N33/545
CPC分类号： G01N33/542 , G01N33/54313
摘要： The invention provides a sensing device comprising: a vessel; a plurality of sensor beads located within the vessel to form interstitial spaces there through; and a plurality of biomolecules bound to at least at portion of the plurality of beads, each of the biomolecules having a fluorescent tag. A sensing device comprised a suspension of a plurality of sensor beads. The invention also provides a method for detecting the binding of two biomolecules comprising the following steps: providing a plurality of first biomolecules, each of the first biomolecules having a first fluorescent tag; and providing a plurality of second biomolecules, each of the second biomolecules having a second fluorescent tag. The binding step have a pre-complexing total fluorescence and detecting any difference between the pre-complexing total fluorescence and the post-complexing total fluorescence.
摘要翻译：本发明提供一种感测装置，包括：容器; 位于容器内的多个传感器珠，以在其间形成间隙空间; 并且多个生物分子结合到所述多个珠粒的至少一部分上，每个生物分子具有荧光标签。感测装置包括多个传感器珠的悬架。本发明还提供了检测两种生物分子的结合的方法，包括以下步骤：提供多个第一生物分子，每个第一生物分子具有第一荧光标记; 并且提供多个第二生物分子，每个第二生物分子具有第二荧光标记。结合步骤具有预络合的总荧光并检测预络合总荧光和后络合总荧光之间的任何差异。

5. 发明专利

AU3041902A Fluorescence and fret based assays for biomolecules on beads 未知
公开(公告)号：AU3041902A
公开(公告)日：2002-05-21
申请号：AU3041902
申请日：2001-11-06
申请人： SCIENCE AND TECHNOLOGY CORP UN
发明人： BURANDA TIONE , HUANG JINMAN , PEREZ-LUNA VICTOR H , LOPEZ GABRIEL P , SIMONS PETER , SKLAR LARRY A
IPC分类号： G01N21/64 , G01N33/542 , G01N33/543
摘要： The invention provides a sensing device comprising: a vessel; a plurality of sensor beads located within the vessel to form interstitial spaces therethrough; and a plurality of biomolecules bound to at least at portion of the plurality of beads, each of the biomolecules having a fluorescent tag. The invention also provides a method for detecting the binding of two biomolecules comprising the following steps: providing a plurality of first biomolecules, each of the first biomolecules having a first fluorescent tag, each of the first biomolecules being bound to a respective substrate of a plurality of substrate; providing a plurality of second biomolecules, each of the second biomolecules having a second fluorescent tag, binding at least portion of the second biomolecules to at least a portion of the first biomolecules to form complexes, wherein the plurality of first biomolecules and the plurality of second biomolecules prior to the binding step have a pre-complexing total fluorescence and wherein the complexes and free second biomolecules after the binding step have a post-complexing total fluorescence; and detecting any difference between the pre-complexing total fluorescence and the post-complexing total fluorescence. A sensing device comprising a suspension of a plurality of sensor beads; and a plurality of biomolecules bound to at least a portion of the plurality of beads, each of the biomolecules having a fluorescent tag is also provided.

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