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1. 发明申请

WO2007130951A3 METHODS AND APPARATUS FOR SEQUENTIAL AMPLIFICATION REACTIONS 审中-公开
标题翻译：用于顺序放大反应的方法和装置
公开(公告)号：WO2007130951A3
公开(公告)日：2008-01-03
申请号：PCT/US2007067895
申请日：2007-05-01
申请人： CEPHEID , SMITH JOSEPH H , PERSING DAVID H , WORTMAN ALAN , CHANG RONALD , SWENSON DAVID
发明人： SMITH JOSEPH H , PERSING DAVID H , WORTMAN ALAN , CHANG RONALD , SWENSON DAVID
IPC分类号： C12P19/34 , C07H19/00 , C12Q1/68
CPC分类号： C12Q1/686 , B01L3/502738 , B01L7/52 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2400/0644 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2565/629 , C12Q2547/101 , C12Q2537/149 , C12Q2537/143 , C12Q2565/101 , C12Q2527/107
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
摘要翻译：本发明提供了在单个反应室中进行多次扩增反应的方法和装置，其通过连续循环的负载反应混合物，在流体封闭的反应体系中放大和除去废反应混合物。特别地，本发明允许通过在闭环控制下从样品的不同部分连续扩增不同靶多核苷酸而从单个样品扩增多个靶多核苷酸。

2. 发明专利

AU2007248149A2 Methods and apparatus for sequential amplification reactions 未知
公开(公告)号：AU2007248149A2
公开(公告)日：2009-02-26
申请号：AU2007248149
申请日：2007-05-01
申请人： CEPHEID
发明人： WORTMAN ALAN , CHANG RONALD , SMITH JOSEPH H , SWENSON DAVID , PERSING DAVID H
IPC分类号： C12P19/34 , C07H19/00 , C12Q1/68
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

3. 发明专利

AU2007248149B2 Methods and apparatus for sequential amplification reactions 未知
公开(公告)号：AU2007248149B2
公开(公告)日：2013-08-15
申请号：AU2007248149
申请日：2007-05-01
申请人： CEPHEID
发明人： SWENSON DAVID , PERSING DAVID H , CHANG RONALD , SMITH JOSEPH H , WORTMAN ALAN
IPC分类号： C12P19/34 , C07H19/00 , C12Q1/68
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

4. 发明专利

ES2402155T3 Procedimientos y aparato para reacciones de amplificación secuenciales 未知
公开(公告)号：ES2402155T3
公开(公告)日：2013-04-29
申请号：ES07782999
申请日：2007-05-01
申请人： CEPHEID
发明人： SMITH JOSEPH H , PERSING DAVID H , WORTMAN ALAN , CHANG RONALD , SWENSON DAVID
IPC分类号： C12Q1/68 , C12P19/34
摘要： Un procedimiento de determinación de la presencia o ausencia de una pluralidad de diferentespolinucleótidos diana en una muestra realizando amplificaciones sucesivas de diferentes polinucleótidos dediferentes partes de la muestra, comprendiendo el procedimiento las etapas de: (i) suministro de una cámara de reacción selectivamente en comunicación fluida con un depósito de residuos, undepósito de muestras que contiene una muestra, un primer depósito de reactivos que contiene primeros reactivos deamplificación y un segundo depósito de reactivos que contiene segundos reactivos de amplificación; (ii) combinación de una primera parte de la muestra y los primeros reactivos de amplificación para formar unaprimera mezcla de reacción; (iii) sometimiento de la primera mezcla de reacción a condiciones de reacción de amplificación en la cámara dereacción para producir un primer amplicón de uno o más polinucleótidos diana siempre que dichos polinucleótidosestén presentes en la muestra; (iv) transferencia fluida de la primera mezcla de reacción al depósito de residuos; (v) combinación de una segunda parte de la muestra y los segundos reactivos de amplificación para formar unasegunda mezcla de reacción; y (vi) sometimiento de la segunda mezcla de reacción a condiciones de reacción de amplificación en la cámara dereacción para producir un segundo amplicón de uno o más polinucleótidos diana siempre que dichos polinucleótidosestén presentes en la muestra, en el que la detección de los amplicones primero y segundo determina la presencia oausencia de la pluralidad de diferentes polinucleótidos diana en la muestra.

5. 发明专利

CA2653533A1 METHODS AND APPARATUS FOR SEQUENTIAL AMPLIFICATION REACTIONS 未知
公开(公告)号：CA2653533A1
公开(公告)日：2007-11-15
申请号：CA2653533
申请日：2007-05-01
申请人： CEPHEID
发明人： SWENSON DAVID , CHANG RONALD , PERSING DAVID H , SMITH JOSEPH H , WORTMAN ALAN
IPC分类号： C12Q1/68 , C12M1/00 , C12M1/34 , C12M1/38 , C12P19/34 , C12Q1/04 , C40B30/00
摘要： The invention provides methods and apparatus for carrying out multiple am plification reactions in a single reaction chamber by successive cycles of l oading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allow s amplification of a plurality of target polynucleotides from a single sampl e by carrying out under closed-loop control successive amplifications of dif ferent target polynucleotides from different portions of the sample.

6. 发明专利

CA2653533C METHODS AND APPARATUS FOR SEQUENTIAL AMPLIFICATION REACTIONS 未知
公开(公告)号：CA2653533C
公开(公告)日：2015-07-07
申请号：CA2653533
申请日：2007-05-01
申请人： CEPHEID
发明人： SMITH JOSEPH H , PERSING DAVID H , WORTMAN ALAN , CHANG RONALD , SWENSON DAVID
IPC分类号： C12Q1/68 , C12M1/00 , C12M1/34 , C12M1/38 , C12P19/34 , C12Q1/04 , C40B30/00
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

7. 发明专利

AU2007248149A1 Methods and apparatus for sequential amplification reactions 未知
公开(公告)号：AU2007248149A1
公开(公告)日：2007-11-15
申请号：AU2007248149
申请日：2007-05-01
申请人： CEPHEID
发明人： WORTMAN ALAN , CHANG RONALD , SMITH JOSEPH H , SWENSON DAVID , PERSING DAVID H
IPC分类号： C12P19/34 , C07H19/00 , C12Q1/68
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

8. 发明公开

EP2016186A4 METHODS AND APPARATUS FOR SEQUENTIAL AMPLIFICATION REACTIONS 有权
标题翻译：方法和设备对于连续扩增反应
公开(公告)号：EP2016186A4
公开(公告)日：2009-06-03
申请号：EP07782999
申请日：2007-05-01
申请人： CEPHEID
发明人： SMITH JOSEPH H , PERSING DAVID H , WORTMAN ALAN , CHANG RONALD , SWENSON DAVID
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/686 , B01L3/502738 , B01L7/52 , B01L2300/0654 , B01L2300/0816 , B01L2300/0864 , B01L2400/0644 , C12Q1/6848 , C12Q1/6851 , C12Q1/689 , C12Q2565/629 , C12Q2547/101 , C12Q2537/149 , C12Q2537/143 , C12Q2565/101 , C12Q2527/107
摘要： The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.

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