专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2013132347A2 IMPROVED ELISA IMMUNOASSAY FOR CALPROTECTIN 审中-公开
标题翻译：改进的ELISA免疫印迹
公开(公告)号：WO2013132347A2
公开(公告)日：2013-09-12
申请号：PCT/IB2013/000886
申请日：2013-03-05
申请人： CALPRO AS , MØRK, Einar , DALE, Inge , FAGERHOL, Magne, K.
发明人： MØRK, Einar , DALE, Inge , FAGERHOL, Magne, K.
CPC分类号： G01N33/54306 , G01N33/577 , G01N2333/4727 , G01N2800/065
摘要： An improved immunoassay for S100A9 provides increased reproducibility and accuracy for the determination of calprotectin, allowing more accurate diagnosis and screening for inflammatory bowel disease. In particular, the immunoassay is a sandwich immunoassay that uses an unlabeled monoclonal anti-S100A9 antibody as the capture antibody and a labeled polyclonal anti-calprotectin antibody as the detection antibody. Further improvements in reproducibility and accuracy are obtained by using citrate buffer as a coating buffer to coat a solid support, such as used in an ELISA assay, with the capture antibody. In general, the method comprises the steps of: (1 ) providing a monoclonal anti-S100A9 antibody; (2) coating the monoclonal anti-S100A9 antibody onto a solid support; (c) reacting the solid support with coated monoclonal anti-S100A9 antibody with a sample that may contain calprotectin to bind any calprotectin in the sample to the solid support; (d) washing the solid support; (e) reacting the solid support with a labeled polyclonal anti-calprotectin antibody to bind the labeled polyclonal anti-calprotectin antibody to the calprotectin bound to the solid support, the labeled polyclonal antibody being able to produce a detectable signal proportional to the concentration of the antibody bound to the calprotectin bound to the solid support; and (f) measuring the detectable signal to determine the concentration of calprotectin in the sample. The invention further includes diagnostic methods, methods of screening antibodies, and kits.
摘要翻译： S100A9的改进的免疫测定提供了重新测定钙卫蛋白的重现性和准确性，允许更准确地诊断和筛选炎症性肠病。特别地，免疫测定是使用未标记的单克隆抗S100A9抗体作为捕获抗体和标记的多克隆抗钙蛋白抗体作为检测抗体的夹心免疫测定。通过使用柠檬酸盐缓冲液作为包被缓冲液，用捕获抗体涂覆固体支持物（如ELISA测定中使用的）来获得再现性和准确性的进一步改善。通常，该方法包括以下步骤：（1）提供单克隆抗S100A9抗体; （2）将单克隆抗S100A9抗体包被到固体支持物上; （c）使固体支持物与涂覆的单克隆抗S100A9抗体与可含有钙卫蛋白的样品反应以将样品中的任何钙卫蛋白结合至固体支持物; （d）洗涤固体支持物; （e）使固体支持物与标记的多克隆抗钙蛋白抗体反应，以将标记的多克隆抗钙蛋白抗体结合到与固体支持物结合的钙卫蛋白结合物上，标记的多克隆抗体能够产生与浓度为与固定支链结合的钙卫蛋白结合的抗体; 和（f）测量可检测信号以确定样品中钙卫蛋白的浓度。本发明还包括诊断方法，筛选抗体的方法和试剂盒。

IPRDB

热门服务

关于我们

友情链接

联系方式