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1. 发明授权

US08889860B2 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing 有权
标题翻译： 3'-OH未封闭，快速可光切割的终止核苷酸和用于核酸测序的方法
公开(公告)号：US08889860B2
公开(公告)日：2014-11-18
申请号：US13615284
申请日：2012-09-13
申请人： Brian Stupi , Hong Li , Weidong Wu , Megan N. Hersh , David Hertzog , Sidney E. Morris , Michael L. Metzker
发明人： Brian Stupi , Hong Li , Weidong Wu , Megan N. Hersh , David Hertzog , Sidney E. Morris , Michael L. Metzker
IPC分类号： C07D487/00 , C07D239/02 , C07H19/173 , C07H19/20 , C07H19/14 , C12Q1/68 , C07H19/073 , C07H19/10
CPC分类号： C12Q1/6869 , B01L3/5027 , B01L2300/0654 , B01L2300/168 , C07H19/073 , C07H19/10 , C07H19/14 , C07H19/173 , C07H19/20 , C12Q1/6874 , C12Q1/6883 , C12Q2525/117 , C12Q2600/156 , C12Q2600/16
摘要： The present invention relates generally to 3′-OH unblocked, labeled and unlabeled nucleotides and nucleosides with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds, which may be represented by the following formulas: wherein the variables are defined herein, may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
摘要翻译：本发明一般涉及用于与DNA和RNA测序和分析相关的方法和系统中的5'-甲氧基取代的基于硝基苄基的可光切割终止基团的3'-OH未封闭，标记和未标记的核苷酸和核苷。可以由下式表示的这些化合物：其中变量在本文中定义，可用作可逆终止子，因为它们表现出快速的核苷酸掺入动力学，单碱基终止，高核苷酸选择性和快速终止基团切割天然存在的核苷酸。

2. 发明申请

US20130122489A1 3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING 有权
标题翻译： 3'-OH解除封闭，快速的光能终止核酸和核酸序列的方法
公开(公告)号：US20130122489A1
公开(公告)日：2013-05-16
申请号：US13615284
申请日：2012-09-13
申请人： Brian STUPI , Hong Li , Weidong Wu , Megan N. Hersh , David Hertzog , Sidney E. Morris , Michael L. Metzker
发明人： Brian STUPI , Hong Li , Weidong Wu , Megan N. Hersh , David Hertzog , Sidney E. Morris , Michael L. Metzker
IPC分类号： C12Q1/68 , C07H19/10 , C07H19/14
CPC分类号： C12Q1/6869 , B01L3/5027 , B01L2300/0654 , B01L2300/168 , C07H19/073 , C07H19/10 , C07H19/14 , C07H19/173 , C07H19/20 , C12Q1/6874 , C12Q1/6883 , C12Q2525/117 , C12Q2600/156 , C12Q2600/16
摘要： The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide.
摘要翻译：本发明一般涉及用于与DNA和RNA测序和分析相关的方法和系统中使用的5-甲氧基取代的硝基苄基基可光切割终止基团的3'-OH未封闭核苷酸和核苷标记和未标记的核苷。这些化合物可以用作可逆终止子，因为它们表现出快速的核苷酸掺入动力学，单碱基终止，高核苷酸选择性以及导致天然存在的核苷酸的快速终止基团切割。

3. 发明授权

US08497360B2 Nucleotides and nucleosides and methods for their use in DNA sequencing 有权
公开(公告)号：US08497360B2
公开(公告)日：2013-07-30
申请号：US13406934
申请日：2012-02-28
申请人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
发明人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
IPC分类号： C07G11/00 , C07H15/00 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q2525/117 , C12Q2563/107
摘要： The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.

4. 发明申请

US20130035237A1 Nucleotides and Nucleosides and Methods for their Use in DNA Sequencing 有权
标题翻译：核苷酸和核苷及其用于DNA测序的方法
公开(公告)号：US20130035237A1
公开(公告)日：2013-02-07
申请号：US13406934
申请日：2012-02-28
申请人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
发明人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
IPC分类号： C12Q1/68 , C07H19/10 , C40B20/00
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q2525/117 , C12Q2563/107
摘要： The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.
摘要翻译：本发明一般涉及标记和未裂解的可切割终止基团以及用于DNA测序和其他类型的DNA分析的方法。更具体地，本发明部分地涉及具有化学可裂解，可光切割，可酶切割或不可光裂解的基团的核苷酸和核苷，以及它们用于DNA测序的方法及其在生物医学研究中的应用。

5. 发明授权

US08148503B2 Nucleotides and nucleosides and methods for their use in DNA sequencing 有权
标题翻译：核苷酸和核苷及其用于DNA测序的方法
公开(公告)号：US08148503B2
公开(公告)日：2012-04-03
申请号：US12483080
申请日：2009-06-11
申请人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
发明人： Vladislav A. Litosh , Megan N. Hersh , Brian P. Stupi , Weidong Wu , Michael L. Metzker
IPC分类号： C07G11/00 , C07H15/00 , C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6869 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C12Q2525/117 , C12Q2563/107
摘要： The present invention relates generally to labeled and unlabled cleavable terminating groups and methods for DNA sequencing and other types of DNA analysis. More particularly, the invention relates in part to nucleotides and nucleosides with chemically cleavable, photocleavable, enzymatically cleavable, or non-photocleavable groups and methods for their use in DNA sequencing and its application in biomedical research.
摘要翻译：本发明一般涉及标记和未裂解的可切割终止基团以及用于DNA测序和其他类型的DNA分析的方法。更具体地，本发明部分地涉及具有化学可裂解，可光切割，可酶切割或不可光裂解的基团的核苷酸和核苷，以及它们用于DNA测序的方法及其在生物医学研究中的应用。

6. 发明授权

US08969535B2 Photocleavable labeled nucleotides and nucleosides and methods for their use in DNA sequencing 有权
标题翻译：可光清洗标记的核苷酸和核苷及其用于DNA测序的方法
公开(公告)号：US08969535B2
公开(公告)日：2015-03-03
申请号：US13592917
申请日：2012-08-23
申请人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
发明人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
IPC分类号： C07G3/00 , C12Q1/68 , C07H21/04 , C07H19/04
CPC分类号： C07H19/23 , C07H17/02 , C07H19/10 , C07H19/20 , C07H21/04 , C12Q1/6869 , C12Q2535/101
摘要： Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
摘要翻译：提供本文所述的新型核苷酸，核苷及其衍生物，其可用于DNA测序技术和其他类型的DNA分析。在一个实施方案中，具有未被保护的3'-OH基团的核苷酸或核苷酸在核碱基处被衍生化，以包括经由连接体连接至可光切割终止基团的荧光染料。可光致透照荧光基团被设计用于终止DNA合成以及被切割，使得DNA寡聚体可以以并行格式有效排序。在核苷酸和核苷上的快速切割荧光基团的设计可以提高DNA大尺寸寡聚体平行测序的速度和准确性，以便快速进行全基因组测序，以及鉴定多态性和其他有价值的遗传信息，以及允许进一步操纵和分析在荧光基团裂解后的天然状态的核酸分子。

7. 发明授权

US08198029B2 3'-OH unblocked nucleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA sequencing 有权
标题翻译： 3'-OH未封闭的核苷酸和用不可切割的终止基团修饰的核苷碱基及其用于DNA测序的方法
公开(公告)号：US08198029B2
公开(公告)日：2012-06-12
申请号：US12986810
申请日：2011-01-07
申请人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
发明人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
IPC分类号： C12Q1/70 , C12P19/34
CPC分类号： C12Q1/6869 , C07H19/20 , C07H21/04
摘要： Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information.
摘要翻译：提供本文所述的新型核苷酸，核苷及其衍生物，其可用于DNA测序技术和其他类型的DNA分析。在一个实施方案中，具有未保护的3'-OH基团的核苷酸或核苷酸在核碱基处被衍生化，以包括通过接头连接至不可切割终止基团的荧光染料。非可切割荧光基团被设计用于终止DNA合成，使得DNA寡聚体可以以平行格式有效排序。这些试剂和方法将导致更准确地鉴定多态性和其他有价值的遗传信息。

8. 发明授权

US07897737B2 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing 有权
标题翻译： 3'-OH未封端，核苷酸和核苷，用可光裂解修饰的碱基，终止基团及其用于DNA测序的方法
公开(公告)号：US07897737B2
公开(公告)日：2011-03-01
申请号：US11567189
申请日：2006-12-05
申请人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
发明人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
IPC分类号： C07H19/00 , C07H21/00 , C07H19/04 , C07H19/20
CPC分类号： C07H19/23 , C07H17/02 , C07H19/10 , C07H19/20 , C07H21/04 , C12Q1/6869 , C12Q2535/101
摘要： Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
摘要翻译：提供本文所述的新型核苷酸，核苷及其衍生物，其可用于DNA测序技术和其他类型的DNA分析。在一个实施方案中，具有未被保护的3'-OH基团的核苷酸或核苷酸在核碱基处被衍生化，以包括经由连接体连接至可光切割终止基团的荧光染料。可光致透照荧光基团被设计用于终止DNA合成以及被切割，使得DNA寡聚体可以以并行格式有效排序。在核苷酸和核苷上的快速切割荧光基团的设计可以提高DNA大尺寸寡聚体平行测序的速度和准确性，以便快速进行全基因组测序，以及鉴定多态性和其他有价值的遗传信息，以及允许进一步操纵和分析在荧光基团裂解后的天然状态的核酸分子。

9. 发明申请

US20080132692A1 Photocleavable labeled nucleotides and nucleosides and methods for their use in DNA sequencing 有权
标题翻译：可光清洗标记的核苷酸和核苷及其用于DNA测序的方法
公开(公告)号：US20080132692A1
公开(公告)日：2008-06-05
申请号：US11567189
申请日：2006-12-05
申请人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
发明人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
IPC分类号： C07H21/04
CPC分类号： C07H19/23 , C07H17/02 , C07H19/10 , C07H19/20 , C07H21/04 , C12Q1/6869 , C12Q2535/101
摘要： Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a photocleavable terminating group. The photocleavable-fluorescent group is designed to terminate DNA synthesis as well as be cleaved so that DNA oligomers can be sequenced efficiently in a parallel format. The design of such rapidly cleavable fluorescent groups on nucleotides and nucleosides can enhance the speed and accuracy of sequencing of large oligomers of DNA in parallel, to allow rapid whole genome sequencing, and the identification of polymorphisms and other valuable genetic information, as well as allowing further manipulation and analysis of nucleic acid molecules in their native state following cleavage of the fluorescent group.
摘要翻译：提供本文所述的新型核苷酸，核苷及其衍生物，其可用于DNA测序技术和其他类型的DNA分析。在一个实施方案中，具有未被保护的3'-OH基团的核苷酸或核苷酸在核碱基处被衍生化，以包括经由连接体连接至可光切割终止基团的荧光染料。可光致透照荧光基团被设计用于终止DNA合成以及被切割，使得DNA寡聚体可以以并行格式有效排序。在核苷酸和核苷上的快速切割荧光基团的设计可以提高DNA大尺寸寡聚体平行测序的速度和准确性，以便快速进行全基因组测序，以及鉴定多态性和其他有价值的遗传信息，以及允许进一步操纵和分析在荧光基团裂解后的天然状态的核酸分子。

10. 发明申请

US20130095471A1 3'-OH UNBLOCKED NUCLEOTIDES AND NUCLEOSIDES BASE MDIFIED WITH NON-CLEAVABLE, TERMINATING GROUPS AND METHODS FOR THEIR USE IN DNA SEQUENCING 审中-公开
标题翻译： 3'-OH未结核的核酸和核苷酸基因，其具有不可清除的终止组以及用于DNA测序的方法
公开(公告)号：US20130095471A1
公开(公告)日：2013-04-18
申请号：US13467885
申请日：2012-05-09
申请人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
发明人： Weidong Wu , Vladislav A. Litosh , Brian P. Stupi , Michael L. Metzker
IPC分类号： C12Q1/68 , C07H19/20
CPC分类号： C12Q1/6869 , C07H19/20 , C07H21/04
摘要： Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information.
摘要翻译：提供本文所述的新型核苷酸，核苷及其衍生物，其可用于DNA测序技术和其他类型的DNA分析。在一个实施方案中，具有未保护的3'-OH基团的核苷酸或核苷酸在核碱基处被衍生化，以包括通过接头连接至不可切割终止基团的荧光染料。非可切割荧光基团被设计用于终止DNA合成，使得DNA寡聚体可以以平行格式有效排序。这些试剂和方法将导致更准确地鉴定多态性和其他有价值的遗传信息。

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