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1. 发明授权

US07303873B2 Cryptic regulatory elements obtained from plants 失效
标题翻译：从植物获得的隐性调节元件
公开(公告)号：US07303873B2
公开(公告)日：2007-12-04
申请号：US10437261
申请日：2003-05-13
申请人： Brian Miki , Thérèse Ouellet , Jiro Hattori , Elizabeth Foster , Hélène Labbé , Teresa Martin-Heller , Lining Tian , Daniel Charles William Brown , Peijun Zhang , Keqiang Wu
发明人： Brian Miki , Thérèse Ouellet , Jiro Hattori , Elizabeth Foster , Hélène Labbé , Teresa Martin-Heller , Lining Tian , Daniel Charles William Brown , Peijun Zhang , Keqiang Wu
IPC分类号： C12Q1/68 , C12N15/63 , C12N15/82 , C07H21/02 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8234
摘要： T-DNA tagging with a promoterless β-glucuronidase (GUS) gene generated transgenic Nicotiana tabacum plants that expressed GUS activity either only in developing seed coats, or constitutively. Cloning and deletion analysis of the GUS fusion revealed that the promoter responsible for seed coat specificity was located in the plant DNA proximal to the GUS gene. Analysis of the region demonstrated that the seed coat-specificity of GUS expression in this transgenic plant resulted from T-DNA insertion next to a cryptic promoter. This promoter is useful in controlling the expression of genes to the developing seed coat in plant seeds. Similarly, cloning and characterization of the cryptic constitutive promoter revealed the occurrence of several cryptic regulatory regions. These regions include promoter, negative regulatory elements, transcriptional enhancers, core promoter regions, and translational enhancers and other regulatory elements.
摘要翻译：用无启动子β-葡糖醛酸糖苷酶（GUS）基因的T-DNA标签产生转基因烟草植物，其仅在开发种皮或组成型中表达GUS活性。 GUS融合物的克隆和缺失分析表明，负责种衣特异性的启动子位于植物DNA接近GUS基因的位置。该区域的分析表明，该转基因植物中GUS表达的种皮特异性是由隐匿启动子旁边的T-DNA插入产生的。该启动子可用于控制植物种子中发育中的种皮的基因表达。类似地，隐匿性组成型启动子的克隆和表征揭示了几个隐蔽调节区的发生。这些区域包括启动子，负调控元件，转录增强子，核心启动子区和翻译增强子等调控元件。

2. 发明授权

US06784289B2 Translational regulatory elements 失效
标题翻译：翻译监管要素
公开(公告)号：US06784289B2
公开(公告)日：2004-08-31
申请号：US09747007
申请日：2000-12-21
申请人： Thérèse Ouellet , Brian M. Miki , Elizabeth Foster , Teresa Martin-Heller , Lining Tian , Daniel C. Brown , Peijun Zhang , Jiro Hattori , Kamal Malik , Keqiang Wu , David A. Theilmann , Raymond Tropiano
发明人： Thérèse Ouellet , Brian M. Miki , Elizabeth Foster , Teresa Martin-Heller , Lining Tian , Daniel C. Brown , Peijun Zhang , Jiro Hattori , Kamal Malik , Keqiang Wu , David A. Theilmann , Raymond Tropiano
IPC分类号： C07H2104
CPC分类号： C12N15/67 , C12N15/8216
摘要： The present invention is directed to translational regulatory elements that mediate the amount of protein produced within a host capable of expressing a construct comprising one or more translational regulatory elements in operative association with a gene of interest. These translational regulatory elements were derived from T1275 (tCUP) and exhibit a high degree of similarity with members of the RENT family of repetitive elements. Translational regulatory elements are disclosed that either increase or decrease he amount of protein produced within the host organism. These translational elements are operative in a wide range of hosts including plant, animals, yeast, fungi and bacteria. Analogs, derivatives and fragments of these translational elements are also disclosed.
摘要翻译：本发明涉及介导在能够表达包含与感兴趣的基因有效结合的一种或多种翻译调控元件的构建体的宿主内产生的蛋白质的量的翻译调控元件。这些翻译调控元件来自T1275（tCUP），并且与RENT家族的重复元件的成员呈现高程度的相似性。公开了翻译调控元件，即增加或减少宿主生物体内产生的蛋白质的量。这些翻译元件在广泛的宿主中可操作，包括植物，动物，酵母，真菌和细菌。还公开了这些翻译元件的类似物，衍生物和片段。

3. 发明申请

US20050055742A1 Plant regulatory element 审中-公开
标题翻译：植物调节元素
公开(公告)号：US20050055742A1
公开(公告)日：2005-03-10
申请号：US10866529
申请日：2004-06-10
申请人： Brian Miki , Jiro Hattori , Teresa Martin-Heller , Helene Labbe , Kamal Malik , Elizabeth Foster , Keqiang Wu , Daniel Brown , Lining Tian , Therese Ouellet , Peijun Zhang , Elizabeth James , Pierre Fobert , Venkatram Iyer
发明人： Brian Miki , Jiro Hattori , Teresa Martin-Heller , Helene Labbe , Kamal Malik , Elizabeth Foster , Keqiang Wu , Daniel Brown , Lining Tian , Therese Ouellet , Peijun Zhang , Elizabeth James , Pierre Fobert , Venkatram Iyer
IPC分类号： C07K14/415 , C12N15/82 , C12Q1/68 , A01H1/00 , C07H21/04 , C12N5/04
CPC分类号： C07K14/415 , C12N15/8216
摘要： An nucleotide sequence and that exhibits regulatory element activity is disclosed. The nucleotide sequence may be defined by SEQ ID NO:22, a nucleotide sequence that hybridizes to the nucleic acid sequence of SEQ ID NO:22, or a compliment thereof. Also disclosed is a chimeric construct comprising the nucleotide sequence operatively linked with a coding region of interest. A method of expressing a coding region of interest within a plant by introducing the chimeric construct described above, into the plant, and expressing the coding region of interest is also provided. Also disclosed are plants, seed, or plant cells comprising the chimeric construct as defined above.
摘要翻译：公开了核苷酸序列并表现出调节元件活性。核苷酸序列可以由SEQ ID NO：22定义，与SEQ ID NO：22的核酸序列杂交的核苷酸序列或其补体。还公开了包含与感兴趣的编码区可操作地连接的核苷酸序列的嵌合构建体。还提供了通过将上述嵌合构建体引入植物并表达感兴趣的编码区而在植物内表达感兴趣的编码区的方法。还公开了包含如上定义的嵌合构建体的植物，种子或植物细胞。

4. 发明授权

US06939958B1 Regulatory region of a lipid transfer protein (LtpW1) from aleuron tissue of wheat 失效
标题翻译：来自小麦糊粉组织的脂质转移蛋白（LtpW1）的调节区
公开(公告)号：US06939958B1
公开(公告)日：2005-09-06
申请号：US10110637
申请日：2000-10-13
申请人： John Simmonds , Leslie Cass , Linda Harris , Sharon Allard , Kamal Malik , Teresa Martin-Heller , Dan Brown , Ming Hu , Brian Miki , Keqiang Wu
发明人： John Simmonds , Leslie Cass , Linda Harris , Sharon Allard , Kamal Malik , Teresa Martin-Heller , Dan Brown , Ming Hu , Brian Miki , Keqiang Wu
IPC分类号： C07K14/415 , C12N15/82 , C07H21/04
CPC分类号： C12N15/8234 , C07K14/415
摘要： This invention is directed to a regulatory region obtained from a wheat aleurone gene LtpW1. This regulatory region, truncated derivatives, mutations, or deletions of this regulatory region, can be used to express heterologous genes of interest within aleurone cells of a plant. Furthermore, this invention is directed to a truncated LtpW1 regulatory region that exhibits constitutive activity with both monocot and dicot plants. This invention is also directed to vectors comprising these regulatory regions operatively linked with a heterologous gene of interest, as well as plant cell cultures and transgenic plants comprising these vectors. A method for the preparation of a plant using the regulatory regions of this invention are also disclosed.
摘要翻译：本发明涉及从小麦糊粉基因LtpW1获得的调节区。该调节区域，该调节区的截短的衍生物，突变或缺失可用于在植物的糊粉母细胞内表达感兴趣的异源基因。此外，本发明涉及一种截短的LtpW1调节区，其表现出单子叶植物和双子叶植物的组成活性。本发明还涉及包含与感兴趣的异源基因可操作地连接的这些调节区的载体，以及包含这些载体的植物细胞培养物和转基因植物。还公开了使用本发明的调节区制备植物的方法。

5. 发明授权

US06376747B1 Plant-derived map kinase kinase 失效
标题翻译：植物衍生的地图激酶激酶
公开(公告)号：US06376747B1
公开(公告)日：2002-04-23
申请号：US09384162
申请日：1999-08-27
申请人： Ti Xing , Kamal Malik , Teresa Martin-Heller , Brian L. Miki
发明人： Ti Xing , Kamal Malik , Teresa Martin-Heller , Brian L. Miki
IPC分类号： C12N1509
CPC分类号： C12N9/1205 , C12N15/8271 , C12N15/8281
摘要： A mitogen-activated protein (MAP) kinase kinase gene, tMEK2, was isolated from tomato cv. Bonny Best. By mutagenesis, a permanently-active variant, tMEK2MUT, was created. Both wild type tMEK2 and mutant tMEK2MUT were driven by a strong constitutive promoter, tCUP&Dgr;, in a tomato protoplast transient expression system. Pathogenesis-related genes, PR1b1 and PR3, and a wound-inducible gene, ER5, were activated by tMEK2MUT expression revealing the convergence of the signal transduction pathways for pathogen attack and mechanical stress at the level of MAPKK. Activation of biotic and abiotic stress response genes downstream of tMEK2 occurred through divergent pathways involving at least two classes of mitogen-activated protein kinase. This study shows that tMEK2 may play an important role in the interaction of signal transduction pathways that mediate responses to both biotic (eg disease) and abiotic (wound responsiveness) stresses.
摘要翻译：从番茄cv中分离出丝裂原活化蛋白（MAP）激酶激酶基因tMEK2。邦尼最好的通过诱变，创建了永久活跃的变体tMEK2MUT。野生型tMEK2和突变体tMEK2MUT均在番茄原生质体瞬时表达系统中由强组成型启动子tCUPDELTA驱动。发病相关基因PR1b1和PR3以及伤口诱导基因ER5被tMEK2MUT表达活化，表明MAPKK水平的病原体攻击和机械应激信号转导通路的收敛。通过涉及至少两类丝裂原活化蛋白激酶的分歧途径，激活tMEK2下游的生物和非生物胁迫反应基因。这项研究表明，tMEK2可能在介导生物（如疾病）和非生物（伤口反应））应激反应的信号转导途径的相互作用中起重要作用。

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