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1. 发明授权

US08541005B2 Cysteine-tagged streptococcal protein G variant 有权
标题翻译：半胱氨酸标记的链球菌蛋白G变体
公开(公告)号：US08541005B2
公开(公告)日：2013-09-24
申请号：US12306903
申请日：2007-06-20
申请人： Bong Hyun Chung , Jeong Min Lee , Sun-Ok Jung , Yongwon Jung
发明人： Bong Hyun Chung , Jeong Min Lee , Sun-Ok Jung , Yongwon Jung
IPC分类号： A61K39/09 , A61K39/085 , C12Q1/00
CPC分类号： C07K14/31 , C07K17/00 , C07K2319/00 , C07K2319/20 , G01N33/54366 , G01N33/54393 , G01N33/56938 , G01N33/6854 , G01N2333/31 , G01N2333/315
摘要： The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capability, compared with an untagged protein G variant.
摘要翻译：本发明涉及N-末端半胱氨酸标记的链球菌蛋白G变体。由于变体以定向方式结合生物芯片或生物传感器的表面，与未标记的蛋白G变体相比，该变体提供具有改进的抗体固定能力的生物芯片和生物传感器。

2. 发明申请

US20100173430A1 CYSTEINE-TAGGED STAPHYLOCOCCAL PROTEIN G VARIANT 有权
公开(公告)号：US20100173430A1
公开(公告)日：2010-07-08
申请号：US12306903
申请日：2007-06-20
申请人： Bong Hyun Chung , Jeong Min Lee , Sun-Ok Jung , Yongwon Jung
发明人： Bong Hyun Chung , Jeong Min Lee , Sun-Ok Jung , Yongwon Jung
IPC分类号： G01N33/543 , C07K14/315 , C12N15/31 , C12N15/63 , C12N1/00 , C12P21/00 , G01N33/00
CPC分类号： C07K14/31 , C07K17/00 , C07K2319/00 , C07K2319/20 , G01N33/54366 , G01N33/54393 , G01N33/56938 , G01N33/6854 , G01N2333/31 , G01N2333/315
摘要： The present invention relates to an N-terminal cysteine-tagged Streptococcal protein G variant. Since the variant binds in a directional manner to a surface of a biochip or a biosensor, the variant provides a biochip and a biosensor having an improved antibody-immobilizing capability, compared with an untagged protein G variant.
摘要翻译：本发明涉及N-末端半胱氨酸标记的链球菌蛋白G变体。由于变体以定向方式结合生物芯片或生物传感器的表面，与未标记的蛋白G变体相比，该变体提供具有改进的抗体固定能力的生物芯片和生物传感器。

3. 发明申请

US20100209945A1 METHOD FOR PREPARING ANTIBODY MONOLAYERS WHICH HAVE CONTROLLED ORIENTATION USING PEPTIDE HYBRID 有权
标题翻译：使用肽混合物制备具有控制方向的抗体单体的方法
公开(公告)号：US20100209945A1
公开(公告)日：2010-08-19
申请号：US12679982
申请日：2007-10-18
申请人： Sang Jeon Chung , Bong Hyun Chung , Yongwon Jung , Hyo Jin Kang , Jeong Min Lee
发明人： Sang Jeon Chung , Bong Hyun Chung , Yongwon Jung , Hyo Jin Kang , Jeong Min Lee
IPC分类号： G01N33/53 , C07K7/08 , C07K7/06 , G01N1/31
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.
摘要翻译：本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说是用于蛋白质固定的肽杂合物，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。用于本发明的蛋白质固定的肽杂合物有助于控制抗体在各种固体表面上的取向，并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

4. 发明授权

US08236509B2 Method for preparing antibody monolayers which have controlled orientation using peptide hybrid 有权
标题翻译：使用肽杂交制备具有受控取向的抗体单层的方法
公开(公告)号：US08236509B2
公开(公告)日：2012-08-07
申请号：US12679982
申请日：2007-10-18
申请人： Sang Jeon Chung , Bong Hyun Chung , Yongwon Jung , Hyo Jin Kang , Jeong Min Lee
发明人： Sang Jeon Chung , Bong Hyun Chung , Yongwon Jung , Hyo Jin Kang , Jeong Min Lee
IPC分类号： G01N33/53
CPC分类号： C07K17/00 , C07K7/02 , C07K7/08 , G01N33/54353 , G01N33/54393
摘要： The present invention relates to a method for preparing an protein monolayer using a peptide hybrid for protein immobilization, more precisely a peptide hybrid for protein immobilization which has improved solubility by introducing a PEG linker and a proper reaction group to the oligopeptide having specific affinity to selected types of proteins and is designed to provide enough space between solid substrates and proteins immobilized, whereby various solid substrates treated by the hybrid catch specific proteins effectively on. The peptide hybrid for protein immobilization of the present invention facilitates the control of orientation of an antibody on various solid surfaces and immobilization of various antibodies of different origins or having different isotypes with different affinity. Therefore, the surface treatment technique using the peptide hybrid of the invention can be effectively used for the production of various immunosensors and immune chips.
摘要翻译：本发明涉及一种使用肽杂交体制备蛋白质单层的方法，更准确地说，用于蛋白质固定的肽杂交体，其通过将PEG接头和适当的反应基团引入具有对所选择的特异性亲和力的寡肽而具有改善的溶解性蛋白质类型被设计为在固体底物和固定的蛋白质之间提供足够的空间，由此通过混合物处理的各种固体底物有效捕获特异性蛋白质。用于本发明的蛋白质固定化的肽杂合物有助于控制抗体在各种固体表面上的取向并且固定不同来源的各种抗体或具有不同亲和力的不同同种型。因此，使用本发明的肽杂交体的表面处理技术可以有效地用于生产各种免疫传感器和免疫芯片。

5. 发明申请

US20100203653A1 Protein G-Oligonucleotide Conjugate 审中-公开
标题翻译：蛋白G-寡核苷酸缀合物
公开(公告)号：US20100203653A1
公开(公告)日：2010-08-12
申请号：US12665397
申请日：2008-05-16
申请人： Bong Hyun Chung , Yong Won Jung , Jeong Min Lee
发明人： Bong Hyun Chung , Yong Won Jung , Jeong Min Lee
IPC分类号： G01N33/543 , C07K14/00
CPC分类号： C07K19/00 , C07K14/315 , G01N33/54393
摘要： The present invention relates to a protein G conjugate, which is prepared by linking an N-terminal cysteine-tagged protein G variant with an oligonucleotide via a linker. The conjugate binds in a directional manner on the surface of a biochip and biosensor, thereby providing a biochip and biosensor having improved antibody immobilization ability.
摘要翻译：本发明涉及蛋白G缀合物，其通过将N-末端半胱氨酸标记的蛋白G变体与寡核苷酸通过接头连接来制备。缀合物以有向的方式结合在生物芯片和生物传感器的表面上，从而提供具有改进的抗体固定能力的生物芯片和生物传感器。

6. 发明申请

US20100204444A1 MULTIFUNCTIONAL PROTEIN SIMULTANEOUSLY DELIVERING ANTIBODIES AND NANOPARTICLES 审中-公开
标题翻译：多功能蛋白质同时传递抗体和纳米颗粒
公开(公告)号：US20100204444A1
公开(公告)日：2010-08-12
申请号：US12670245
申请日：2008-07-25
申请人： Bong Hyun Chung , Yong Taik Lim , Mi Young Cho , Jeong Min Lee
发明人： Bong Hyun Chung , Yong Taik Lim , Mi Young Cho , Jeong Min Lee
IPC分类号： C07K16/00 , C07K2/00 , C12N9/96 , C07K7/06 , C07H21/00 , C12N15/63 , C12P21/06 , C12N1/00
CPC分类号： C07K14/31 , C07K2319/03 , C07K2319/09
摘要： The present invention relates to a polypeptide structure which can simultaneously deliver an antibody and a nanoparticle into cells, more specifically, to a polypeptide structure for intracellular delivery of an antibody and a nanoparticle, which comprises a nanoparticle-binding region, an antibody-binding region and a signaling capable of delivering substances into cells.
摘要翻译：本发明涉及一种多肽结构，其可以将抗体和纳米颗粒同时递送到细胞中，更具体地涉及用于细胞内递送抗体和纳米颗粒的多肽结构，其包含纳米颗粒结合区，抗体结合区以及能够将物质输送到细胞中的信号。

7. 发明申请

US20110098197A1 Method for Specific Covalent Coupling of Antibody Using a Photoactivable Protein G Variant 审中-公开
标题翻译：使用可光化蛋白G变体的抗体特异性共价偶联的方法
公开(公告)号：US20110098197A1
公开(公告)日：2011-04-28
申请号：US12988722
申请日：2008-05-27
申请人： Bong Hyun Chung , Yong Won Jung , Jeong Min Lee
发明人： Bong Hyun Chung , Yong Won Jung , Jeong Min Lee
IPC分类号： C40B30/04 , C07K19/00 , C12N9/96 , C07K1/00 , C40B40/10 , C40B50/00
CPC分类号： C07K14/315 , G01N33/531 , G01N33/533
摘要： The present invention relates to a protein G variant comprising a mutated Fc binding domain, which is prepared by substituting cysteine for specific residues of the Fc-binding domain of protein G, and a method for preparing the same. Further, the present invention relates to a protein G variant comprising a cysteine mutated Fc binding domain that is site-selectively modified with a UV cross-linker. Further, the present invention relates to a method for UV cross-linking the protein G variant with antibody. The present invention relates to a protein G variant-antibody conjugate that is prepared by the above method. Further, the present invention provides a method for screening or analyzing antigens using the conjugate. Furthermore, the present invention provides a biochip or biosensor fabricated by linking the protein G variant to the surface of a solid support, and a method for fabricating the same. In addition, the present invention provides a method for immobilizing antibodies and analyzing antigens using the biochip or biosensor.
摘要翻译：本发明涉及包含通过用半胱氨酸代替蛋白G的Fc结合结构域的特异性残基制备的突变Fc结合结构域的蛋白G变体及其制备方法。此外，本发明涉及包含用UV交联剂位点选择性修饰的半胱氨酸突变的Fc结合结构域的蛋白G变体。此外，本发明涉及一种用抗体将蛋白G变体UV交联的方法。本发明涉及通过上述方法制备的蛋白G变体 - 抗体缀合物。此外，本发明提供了使用该缀合物筛选或分析抗原的方法。此外，本发明提供了通过将蛋白G变体与固体支持物的表面连接而制造的生物芯片或生物传感器及其制造方法。此外，本发明提供了使用生物芯片或生物传感器固定抗体和分析抗原的方法。

8. 发明授权

US08734762B2 Perfluorocarbon nano-emulsion containing quantum dot nanoparticles and method for preparing the same 有权
标题翻译：含有量子点纳米粒子的全氟化碳纳米乳液及其制备方法
公开(公告)号：US08734762B2
公开(公告)日：2014-05-27
申请号：US12738394
申请日：2008-08-20
申请人： Bong Hyun Chung , Yong Taik Lim , Bang Sil Choi , Jung Hyun Han
发明人： Bong Hyun Chung , Yong Taik Lim , Bang Sil Choi , Jung Hyun Han
IPC分类号： A61B5/00 , A61B8/00 , A61K9/66
CPC分类号： C09K11/565 , C09K11/02 , C09K11/883
摘要： The present invention relates to a perfluorocarbon nanoemulsion containing quantum dot nanoparticles and a method for preparing the same, more specifically, relates to a method for preparing a multifunctional perfluorocarbon nanoemulsion which simultaneously shows the fluorine-based magnetic resonance properties of perfluorocarbon and the optical properties of quantum dot nanoparticles, the method comprising dispersing quantum dot nanoparticles, modified with a fluorine end group, in perfluorocarbon, and then adding an aqueous emulsifier solution containing lipid to the dispersion. The disclosed perfluorocarbon nanoemulsion containing quantum dot nanoparticles has excellent biocompatibility and simultaneously shows optical properties and magnetic resonance properties, and thus can be used as a contrast agent for multi-mode molecular imaging. Accordingly, the nanoemulsion can be used to study cell and small-animal molecular imaging in various fields, including biological and medical fields.
摘要翻译：本发明涉及含有量子点纳米粒子的全氟碳纳米乳液及其制备方法，更具体地涉及制备多功能全氟碳纳米乳液的方法，该方法同时显示全氟化碳的氟基磁共振性能和光学性能量子点纳米粒子，该方法包括将用氟端基改性的量子点纳米粒子分散在全氟化碳中，然后向分散体中加入含有脂质的水性乳化剂溶液。所公开的含有量子点纳米粒子的全氟化碳纳米乳液具有优异的生物相容性，并同时显示光学性质和磁共振性质，因此可用作多模式分子成像的造影剂。因此，纳米乳液可用于研究包括生物和医学领域在内的各个领域的细胞和小动物分子成像。

9. 发明申请

US20130149374A1 Asymmetric Liposomes for the Highly Efficient Encapsulation of Nucleic Acids and Hydrophilic Anionic Compounds, and Method for Preparing Same 有权
标题翻译：用于高效包封核酸和亲水阴离子化合物的不对称脂质体及其制备方法
公开(公告)号：US20130149374A1
公开(公告)日：2013-06-13
申请号：US13697705
申请日：2010-11-12
申请人： Myung Kyu Lee , Abbas Mokhtarieh Amir , Bong Hyun Chung , Jong Min Choi
发明人： Myung Kyu Lee , Abbas Mokhtarieh Amir , Bong Hyun Chung , Jong Min Choi
IPC分类号： A61K9/127 , A61K31/713
CPC分类号： A61K9/1272 , A61K9/1271 , A61K9/1277 , A61K31/122 , A61K31/70 , A61K31/713
摘要： The present invention relates to asymmetric liposomes for high encapsulation efficiency of nucleic acids and hydrophilic anionic compounds, and to a method for preparing same, and specifically, to asymmetric liposomes consisting of a cationic lipid having a small head group as an internal lipid and a neutral or PEGylated lipid having a big head group as an external lipid, wherein nucleic acids and/or anionic compounds are encapsulated in the internal lipid. According to the present invention, asymmetric liposomes, in which nucleic acids and hydrophilic anionic compounds are encapsulated with high efficiency, may be prepared, and thus the same may be used for various purposes, such as gene therapy, and the delivery of hydrophilic anionic drugs which are difficult to prepare as prodrugs, and drug delivery, imaging, etc. can be carried out by encapsulating a fluorescent contrast agent in the liposome.
摘要翻译：本发明涉及用于核酸和亲水性阴离子化合物的高包封效率的不对称脂质体及其制备方法，具体涉及由具有小头基团作为内部脂质和中性的阳离子脂质组成的不对称脂质体或具有大头基的聚乙二醇化脂质作为外部脂质，其中核酸和/或阴离子化合物包封在内部脂质中。根据本发明，可以制备其中核酸和亲水性阴离子化合物以高效率包封的不对称脂质体，因此可以用于各种目的，例如基因治疗和递送亲水性阴离子药物其难以作为前药制备，并且药物递送，成像等可以通过将荧光对比剂包封在脂质体中来进行。

10. 发明申请

US20120220734A1 Reversible Fluorescence Photoswitch based on Dye-Crosslinked Dendritic Nanoclusters for High-Contrast Imaging of Living Biological Systems 有权
标题翻译：基于染料交联树枝状纳米团簇的可逆荧光光电开关，用于生物生物系统的高对比度成像
公开(公告)号：US20120220734A1
公开(公告)日：2012-08-30
申请号：US13218608
申请日：2011-08-26
申请人： Yoonkyung Kim , Hye-Youn Jung , Bong Hyun Chung
发明人： Yoonkyung Kim , Hye-Youn Jung , Bong Hyun Chung
IPC分类号： C09B69/10 , B82Y5/00 , B82Y15/00
CPC分类号： B82Y15/00 , C09B69/00
摘要： The present invention relates to a reversible fluorescence photoswitch based on the dye-crosslinked dendritic nanoclusters for high-contrast imaging of living biological systems. The dendritic nanocluster according to the present invention consists of two or more dendrimers crosslinked each other to have a globular shape overall, and thereby enhancing the fluorescence intensity and improving the detection sensitivity of the monomeric dendrimers. In addition, the dendritic nanocluster according to the present invention was found to internalize into a living zebrafish by both skin permeation and microinjection, independently. Further, the dendritic nanocluster according to the present invention showed low toxicity and thus it could be useful for both in vivo and in vitro imaging as well as the ex vivo cell tracking applications.
摘要翻译：本发明涉及一种基于染料交联树枝状纳米团簇的可逆荧光光电开关，用于生物体系的高对比度成像。根据本发明的树枝状纳米团簇由两个或更多个交联的树枝状聚合物组成，从而整体上具有球状，从而提高荧光强度并提高单体树枝状大分子的检测灵敏度。此外，发现根据本发明的树突状纳米簇独立地通过皮肤渗透和显微注射而内在化为活的斑马鱼。此外，根据本发明的树突状纳米簇显示低毒性，因此其可用于体内和体外成像以及离体细胞追踪应用。

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