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1. 发明专利

JP2009065861A Single nucleotide polymorphism related to bovine fat-crossing and utilization thereof 有权
标题翻译：与牛肉相关的单核多糖多态性及其利用
公开(公告)号：JP2009065861A
公开(公告)日：2009-04-02
申请号：JP2007235628
申请日：2007-09-11
申请人： Big Kenkyusho Kk , Kyoto Univ , Livestock Improvement Association Of Japan Inc , Nippon Meat Packers Inc , Ooita Ken , ビッグ研究所株式会社 , 国立大学法人京都大学 , 大分県 , 日本ハム株式会社 , 社団法人家畜改良事業団
发明人： YAMADA YOSHINAGA , MIYAKE TAKESHI , TANIGUCHI YUKIO , SASAKI YOSHIYUKI , MORITA MITSUO , SASAKI SEIKI , ITO TOMOHITO , MURAKAMI HIROSHI , SUKEGAWA SHIN , TAKAHAGI YOICHI , MORIMATSU FUMITAKE , FUJITA TATSUO , WATANABE NAOTO , ITO MASAYUKI , SHIGA KAZUO
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a method for predicting the bovine fat-crossing ability by judging the bovine fat-crossing ability according to a genotype analysis, to provide a method for determining the genotype and to provide a kit used therefor.
SOLUTION: The SNP (single nucleotide polymorphism) in at least one site ranging from the first exon initiating point to the -1,736 bp site of bovine EDG1 gene, from the first exon initiating point to the -652 bp site of bovine TTN gene or from the first exon initiating point to the +22,220 bp site of bovine MBL1 gene is analyzed by PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism). Thereby, the bovine fat-crossing ability is predicted and the genotype is determined.
COPYRIGHT: (C)2009,JPO&INPIT
摘要翻译：待解决的问题：提供一种通过根据基因型分析判断牛脂肪交叉能力来预测牛脂肪交叉能力的方法，以提供确定基因型的方法并提供用于其的试剂盒。解决方案：从第一外显子起始点到牛EDG1基因的第一个外显子起始点到第一个外显子起始点至-652bp位点的至少一个位点处的SNP（单核苷酸多态性）基因或从第一外显子起始点到牛MBL1基因的+22,220bp位点通过PCR-RFLP（聚合酶链反应 - 限制性片段长度多态性）进行分析。因此，预测牛的脂肪交叉能力，并确定基因型。版权所有（C）2009，JPO＆INPIT

2. 发明专利

JP2010187636A Single nucleotide polymorphism associated with bovine fat-crossing and utilization of the same 有权
标题翻译：与牛肉相关的单核多糖多态性与其相同的交叉和利用
公开(公告)号：JP2010187636A
公开(公告)日：2010-09-02
申请号：JP2009038007
申请日：2009-02-20
申请人： Big Kenkyusho Kk , Kyoto Univ , Livestock Improvement Association Of Japan Inc , Nippon Meat Packers Inc , Oita Prefecture , ビッグ研究所株式会社 , 国立大学法人京都大学 , 大分県 , 日本ハム株式会社 , 社団法人家畜改良事業団
发明人： YAMADA YOSHINAGA , MIYAKE TAKESHI , TANIGUCHI YUKIO , SASAKI YOSHIYUKI , MORITA MITSUO , SASAKI SEIKI , MURAKAMI HIROSHI , SUKEGAWA SHIN , TAKAHAGI YOICHI , MORIMATSU FUMITAKE , FUJITA TATSUO , WATANABE NAOTO , SHIGA KAZUO
IPC分类号： C12N15/09 , C12Q1/68
摘要： PROBLEM TO BE SOLVED: To provide a prediction method of a bovine fat-crossing capability for judging bovine fat-crossing capability according to genotype analyses, a method for judging a genotype and a kit used for the method.
SOLUTION: An SNP type present in a portion ranging from a starting point of a first exon to a -7272bp part of a bovine RPL27 gene is analyzed by means of a PCR-RFLP and thereby the bovine fat-crossing capability is predicted and its genotype is judged.
COPYRIGHT: (C)2010,JPO&INPIT
摘要翻译：待解决的问题：提供根据基因型分析判断牛脂肪交叉能力的牛脂肪交叉能力的预测方法，用于判断基因型的方法和用于该方法的试剂盒。解决方案：通过PCR-RFLP分析存在于从第一外显子的起始点到牛RPL27基因的-7272bp部分的部分的SNP型，从而预测牛脂肪交叉能力并判断其基因型。版权所有（C）2010，JPO＆INPIT

3. 发明专利

JP2012170380A Method for quickly and easily discriminating salmonella serovar by multiple pcr 有权
标题翻译：通过多重PCR快速分析沙门氏菌的方法
公开(公告)号：JP2012170380A
公开(公告)日：2012-09-10
申请号：JP2011034184
申请日：2011-02-21
申请人： Nippon Meat Packers Inc , 日本ハム株式会社
发明人： NISHIYAMA YASUTAKA , TAKAHAGI YOICHI , SUKEGAWA SHIN
IPC分类号： C12Q1/68 , C12N15/09
CPC分类号： Y02A50/451
摘要： PROBLEM TO BE SOLVED: To provide a multiple detection method capable of discriminating serovars (Enteritidis, Infantis, Typhimurium, Choleraesuis, Dublin, Heidelberg, and Schwarzengrund) among Salmonellas leading to troubles mainly in a stock raising site; and an easy discrimination method capable of directly performing multiple polymerase chain reaction (PCR) without isolating Salmonellas from a test sample-derived enrichment culture solution.SOLUTION: The multiple PCR serovar discrimination method is characterized by the use of a primer set for amplifying Salmonella-specific invA genes as well as primer sets with specific sequences specific to respective serovars, primer sets specific to Typhimurium, specific to Infantis, specific to Choleraesuis, specific to Dublin, specific to Heidelberg and specific to Schwarzengrund.
摘要翻译：要解决的问题：提供能够区分主要在库存场所导致麻烦的沙门氏菌中的血清型（肠炎沙门菌属，婴儿动物，鼠伤寒沙门氏菌，都柏林，海德堡和Schwarzengrund）的多重检测方法; 以及能够直接进行多种聚合酶链反应（PCR）而不从测试样品衍生的富集培养液中分离沙门氏菌的易于鉴别的方法。解决方案：多重PCR血清型鉴别方法的特征在于使用用于扩增沙门氏菌特异性invA基因的引物组以及针对特定于各种血清型的特异性序列的引物组，特异于疟原虫的Typhimurium特异性引物组，专门针对都柏林的霍乱虫属，特定于海德堡，具体针对Schwarzengrund。版权所有（C）2012，JPO＆INPIT

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