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1. 发明申请

US20170260497A1 THERMORESPONSIVE CELL CULTURE SUPPORTS 审中-公开
公开(公告)号：US20170260497A1
公开(公告)日：2017-09-14
申请号：US15499964
申请日：2017-04-28
申请人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
发明人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
IPC分类号： C12N5/00 , C12N5/0775
CPC分类号： C12N5/0068 , C12N5/0662 , C12N5/0663 , C12N5/0664 , C12N5/0665 , C12N5/0666 , C12N5/0667 , C12N5/0668 , C12N2533/12 , C12N2539/10
摘要： The present invention relates to a cell culture support including a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer includes at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex including: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further include medium, to provide a cell culture complex.

2. 发明授权

US10550366B2 Thermoresponsive cell culture supports 有权
公开(公告)号：US10550366B2
公开(公告)日：2020-02-04
申请号：US15499964
申请日：2017-04-28
申请人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
发明人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
IPC分类号： C12N5/00 , C12N5/0775
摘要： The present invention relates to a cell culture support including a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer includes at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex including: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further include medium, to provide a cell culture complex.

3. 发明授权

US09701939B2 Thermoresponsive cell culture supports 有权
公开(公告)号：US09701939B2
公开(公告)日：2017-07-11
申请号：US14110465
申请日：2012-04-06
申请人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
发明人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
IPC分类号： C12N5/0775 , C12N5/00
CPC分类号： C12N5/0068 , C12N5/0662 , C12N5/0663 , C12N5/0664 , C12N5/0665 , C12N5/0666 , C12N5/0667 , C12N5/0668 , C12N2533/12 , C12N2539/10
摘要： The present invention relates to a cell culture support comprising a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex.

4. 发明申请

US20140193911A1 THERMORESPONSIVE CELL CULTURE SUPPORTS 有权
标题翻译：热源细胞培养支持
公开(公告)号：US20140193911A1
公开(公告)日：2014-07-10
申请号：US14110465
申请日：2012-04-06
申请人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
发明人： Bi-Min Newby , Nikul Patel , John Cavicchia , Ge Zhang
IPC分类号： C12N5/00 , C12N5/0775
CPC分类号： C12N5/0068 , C12N5/0662 , C12N5/0663 , C12N5/0664 , C12N5/0665 , C12N5/0666 , C12N5/0667 , C12N5/0668 , C12N2533/12 , C12N2539/10
摘要： The present invention relates to a cell culture support comprising a substrate and a thermoresponsive polymeric blend layer, wherein the polymeric blend layer comprises at least one thermoresponsive polymer and at least one network forming adhesion promoter. The present invention further relates a method of making a cell culture complex comprising: providing a substrate; blending at least one thermoresponsive polymer and at least one network forming adhesion promoter to provide a polymeric blend; applying a thin film of said polymeric blend to the substrate to provide a polymeric blend layer on the substrate; curing the polymeric blend layer on the substrate to provide a cell culture support; and depositing cells onto said cell culture support, wherein the cells may optionally further comprise medium, to provide a cell culture complex.
摘要翻译：本发明涉及一种细胞培养载体，其包括基质和热反应性聚合物共混物层，其中所述聚合共混物层包含至少一种热响应聚合物和至少一种形成网络的粘合促进剂。本发明还涉及一种制备细胞培养复合物的方法，包括：提供底物; 混合至少一种热可聚合物和至少一种形成网络的粘合促进剂以提供聚合物共混物; 将所述聚合物共混物的薄膜施加到所述基底上以在所述基底上提供聚合物共混物层; 固化基底上的聚合物共混物层以提供细胞培养支持物; 以及将细胞沉积在所述细胞培养支持物上，其中所述细胞可以任选地进一步包含培养基，以提供细胞培养物复合物。

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