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    • 6. 发明申请
    • Potassium channels, nucleotide sequences encoding them, and methods of using same
    • 钾通道,编码它们的核苷酸序列及其使用方法
    • US20060110792A1
    • 2006-05-25
    • US11178538
    • 2005-07-12
    • Mark PauschLaura Price
    • Mark PauschLaura Price
    • C07K14/705C07H21/04C12P21/06
    • C07K14/705C07K14/43545C07K14/43581C07K14/47C12Q1/025G01N33/6872
    • This invention relates generally to a new family of potassium channels. More particularly, the present invention relates to the cloning and characterization of a family of distinct trans-membrane potassium ion channels, characterization of such channels, newly identified polynucleotide sequences, polypeptides encoded by such sequences, expression vectors capable of heterologous expression of such polynucleotide sequences, transformed host cells containing the expression vectors, and assay methods and kits therefor for determining the expression of heterologous nucleotide sequences encoding all or a portion of said potassium channels in host cells, chromosome mapping, diagnostic methodologies and kits therefore. Genes encoding potassium channels representative of this family were cloned from Drosophila melanogaster, Caenorhabditis elegans, human and mouse ESTs, and human brain, heart and kidney cDNA libraries. More particularly, the invention arises in part from the determination that the DNA sequences of these genes encode a structurally distinct potassium channel whose molecular architecture is characterized by four membrane spanning domains and two putative pore forming domains.
    • 本发明一般涉及新的钾通道家族。 更具体地说,本发明涉及不同跨膜钾离子通道家族的克隆和表征,这些通道的表征,新鉴定的多核苷酸序列,由这些序列编码的多肽,能够异源表达这种多核苷酸序列的表达载体 ,含有表达载体的转化的宿主细胞,以及用于确定宿主细胞中编码所有或部分所述钾通道的异源核苷酸序列的表达的测定方法和试剂盒,因此染色体图谱,诊断方法和试剂盒。 从黑腹果蝇,秀丽隐杆线虫,人和小鼠EST以及人脑,心脏和肾脏cDNA文库克隆了代表该家族的钾通道的基因。 更具体地说,本发明的部分原因在于确定这些基因的DNA序列编码结构不同的钾通道,其分子结构的特征在于四个膜跨越结构域和两个推定的成孔结构域。