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1. 发明申请

US20100251419A1 NOVEL NUCLEIC ACIDS FROM SESAME PLANTS 审中-公开
标题翻译：来自SESAME植物的新型核酸
公开(公告)号：US20100251419A1
公开(公告)日：2010-09-30
申请号：US12797172
申请日：2010-06-09
申请人： Basil S. Shorrosh , Matthew Jenkin , Benyuan Dang
发明人： Basil S. Shorrosh , Matthew Jenkin , Benyuan Dang
IPC分类号： A01H1/00 , C12N15/82 , C12N5/10 , C12N15/63 , C07H21/04 , A01H5/00
CPC分类号： C12N15/8234
摘要： Novel nucleic acids derived from the 5′ untranslated regions of the 2S albumin and 11S globulin genes from sesame are disclosed. These novel nucleic acids can be used to direct expression of coding nucleic acids. Expression constructs can be introduced into a plant cell to generate a transgenic plant cell or to generate a transgenic plant.
摘要翻译：公开了衍生自2S白蛋白的5'非翻译区和来自芝麻的11S球蛋白基因的新型核酸。这些新型核酸可用于指导编码核酸的表达。可以将表达构建体引入植物细胞以产生转基因植物细胞或产生转基因植物。

2. 发明授权

US07750139B2 Nucleic acids from sesame plants 失效
标题翻译：来自芝麻植物的核酸
公开(公告)号：US07750139B2
公开(公告)日：2010-07-06
申请号：US10565898
申请日：2004-07-29
申请人： Basil S. Shorrosh , Matthew Jenkin , Benyuan Dang
发明人： Basil S. Shorrosh , Matthew Jenkin , Benyuan Dang
IPC分类号： C07H21/04 , C12N15/82 , C12N5/10
CPC分类号： C12N15/8234
摘要： Novel nucleic acids derived from the 5′ untranslated regions of the 2S albumin and 11S globulin genes from sesame are disclosed. These novel nucleic acids can be used to direct expression of coding nucleic acids. Expression constructs can be introduced into a plant cell to generate a transgenic plant cell or to generate a transgenic plant.
摘要翻译：公开了衍生自2S白蛋白的5'非翻译区和来自芝麻的11S球蛋白基因的新型核酸。这些新型核酸可用于指导编码核酸的表达。可以将表达构建体引入植物细胞以产生转基因植物细胞或产生转基因植物。

3. 发明授权

US07541519B2 Fatty acid desaturases and mutant sequences thereof 失效
公开(公告)号：US07541519B2
公开(公告)日：2009-06-02
申请号：US11108185
申请日：2005-04-18
申请人： Lorin R. Debonte , Basil S. Shorrosh
发明人： Lorin R. Debonte , Basil S. Shorrosh
IPC分类号： A01H5/00 , A01H1/00
CPC分类号： C12N15/8247 , C07C53/126 , C07C57/02 , C07C57/03 , C12N9/0083
摘要： Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

4. 发明授权

US06995301B1 Plant acyltransferases 有权
标题翻译：植物酰基转移酶
公开(公告)号：US06995301B1
公开(公告)日：2006-02-07
申请号：US09565620
申请日：2000-05-04
申请人： Basil S. Shorrosh
发明人： Basil S. Shorrosh
IPC分类号： A01N5/00 , C12N15/82
CPC分类号： C12N9/1029 , C12N15/8247
摘要： A gene encoding diacylglycerol acyltransferase from Brassica napus is described, as well as methods of altering oil content in plants using the diacylglycerol acyltransferase.
摘要翻译：描述了从甘蓝型油菜中编码二酰基甘油酰基转移酶的基因，以及使用二酰基甘油酰基转移酶改变植物油含量的方法。

5. 发明授权

US06723895B2 Plants containing a cytosolic acetyl CoA-carboxylase nucleic acid 失效
标题翻译：含有胞质乙酰辅酶A羧化酶核酸的植物
公开(公告)号：US06723895B2
公开(公告)日：2004-04-20
申请号：US09839477
申请日：2001-04-20
申请人： Lorin R. DeBonte , Basil S. Shorrosh
发明人： Lorin R. DeBonte , Basil S. Shorrosh
IPC分类号： A01H500
CPC分类号： C12N9/93 , C11B1/10 , C11B3/001 , C12N15/8247
摘要： Plants are disclosed that contain a recombinant nucleic acid construct comprising a nucleic acid encoding a cytosolic acetyl coA-carboxylase (ACCase) operably linked to a promoter. Seeds produced from such plants exhibit statistically significantly increased oil content as compared to seeds produced by a corresponding plant lacking the nucleic acid encoding the ACCase. Methods of producing seeds exhibiting statistically significantly increased oil content are also disclosed.
摘要翻译：公开了含有重组核酸构建体的植物，其包含编码与启动子可操作连接的胞质乙酰辅酶A羧化酶（ACCase）的核酸。与由缺乏编码ACCase的核酸的相应植物产生的种子相比，从这样的植物产生的种子显示出显着增加的油含量。还公开了产生显着增加的油含量的种子的方法。

6. 发明授权

US5962767A Structure and expression of an arabidopsis acetyl-coenzyme A carboxylase gene 失效
标题翻译：拟南芥乙酰辅酶A羧化酶基因的结构和表达
公开(公告)号：US5962767A
公开(公告)日：1999-10-05
申请号：US790519
申请日：1997-01-29
申请人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
发明人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
IPC分类号： C12N9/00 , C12N15/82 , A01H5/00 , A01H5/10 , C12N5/04
CPC分类号： C12N9/93 , C12N15/8247
摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10 kb gene encodes a 251 kD cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
摘要翻译：拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10 kb基因编码251 kD胞质ACCase同工酶。该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

7. 发明授权

US5925805A Methods of increasing oil content of seeds 失效
标题翻译：增加种子油含量的方法
公开(公告)号：US5925805A
公开(公告)日：1999-07-20
申请号：US677010
申请日：1996-07-01
申请人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
发明人： John B. Ohlrogge , Keith R. Roesler , Basil S. Shorrosh
IPC分类号： C12N9/00 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8247 , C12N9/93
摘要： An Arabidopsis acetyl-CoA carboxylase (ACCase) gene has been isolated and sequenced. The 10-kb gene encodes a 251-kd cytosolic ACCase isozyme. The nucleic acid sequence of the gene is set forth in SEQ ID No. 1 and has been accorded GenBank Accession No. L27074. The cDNA sequence and deduced amino acid sequence of the cDNA are set forth in SEQ ID Nos. 2 and 3, respectively. By controlling expression of the gene of the present invention, carboxylation of acetyl-CoA to produce malonyl-CoA may be controlled. Thus, by introducing constructs of the gene of the present invention in sense or anti-sense orientation, carboxylation of acetyl-CoA to produce malonyl-CoA may be increased or decreased. Consequently, fatty acid synthesis and elongation in plants and seeds which depends on malonyl-CoA may also be controlled.
摘要翻译：拟南芥乙酰辅酶A羧化酶（ACCase）基因已被分离并测序。 10-kb基因编码251-kd胞质ACCase同工酶。该基因的核酸序列如SEQ ID No.1所示，并符合GenBank登录号L27074。 cDNA的cDNA序列和推定的氨基酸序列分别列于SEQ ID No.2和3。通过控制本发明的基因的表达，可以控制乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，通过在正义或反义方向引入本发明基因的构建体，可以增加或减少乙酰辅酶A的羧基化以产生丙二酰辅酶A。因此，也可以控制依赖于丙二酰辅酶A的植物和种子中的脂肪酸合成和延伸。

8. 发明授权

US06537750B1 Plant fatty acid desaturase promoters 失效
标题翻译：植物脂肪酸去饱和酶启动子
公开(公告)号：US06537750B1
公开(公告)日：2003-03-25
申请号：US09366715
申请日：1999-08-04
申请人： Basil S. Shorrosh
发明人： Basil S. Shorrosh
IPC分类号： C12Q168
CPC分类号： C12N9/0083 , C12N15/8216 , C12N15/8222 , C12N15/8225 , C12N15/8227 , C12N15/8231 , C12N15/8234
摘要： Fatty acid desaturase 5′ regulatory elements are described, as well as nucleic acid constructs and transgenic plants that include these regulatory elements. Also described are fatty acid desaturase 3′ untranslated regions.
摘要翻译：脂肪酸去饱和酶5'调节元件以及包含这些调节元件的核酸构建体和转基因植物进行了描述。还描述了脂肪酸去饱和酶3'非翻译区。

9. 发明授权

US06342658B1 Fatty acid desaturases and mutant sequences thereof 有权
标题翻译：脂肪酸去饱和酶及其突变体序列
公开(公告)号：US06342658B1
公开(公告)日：2002-01-29
申请号：US09354231
申请日：1999-07-16
申请人： Lorin R. DeBonte , Basil S. Shorrosh
发明人： Lorin R. DeBonte , Basil S. Shorrosh
IPC分类号： A01H400
CPC分类号： C12N9/0083 , C07C53/126 , C07C57/02 , C07C57/03 , C12N15/8247
摘要： Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plants. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.
摘要翻译：提供具有高油酸的种子，植物和油; 低亚油酸; 低亚油酸加亚麻酸; 和有利的功能或营养特性。公开了在δ-12或δ-15脂肪酸去饱和酶基因中含有突变的植物。优选的植物是油菜籽和向日葵植物。携带这种突变基因的植物已经改变了种子中的脂肪酸组成。在一个实施方案中，植物在具有保守基序His-Xaa-Xaa-Xaa-His的区域中含有突变，其在Δ-12和δ-15脂肪酸去饱和酶中发现。优选的基序具有序列His-Glu-Cys-Gly-His。该基序中优选的突变具有氨基酸序列His-Lys-Cys-Gly-His。公开了包含突变体Δ-12或δ-15脂肪酸去饱和酶基因序列的核酸片段。

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