专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US5262313A Carrageeman-immobilized esterase 失效
标题翻译：角叉菜胶固定酯酶
公开(公告)号：US5262313A
公开(公告)日：1993-11-16
申请号：US715829
申请日：1991-06-14
申请人： Barbara S. Kitchell , Robert W. Henkens , Philip Brown , Steven W. Baldwin , Charles H. Lochmuller , John P. O'Daly
发明人： Barbara S. Kitchell , Robert W. Henkens , Philip Brown , Steven W. Baldwin , Charles H. Lochmuller , John P. O'Daly
IPC分类号： C12N11/04 , C12P7/62 , C12N9/20 , C12N11/10 , C12P7/64
CPC分类号： C12N11/04
摘要： Carrageenan-immobilized enzymes are prepared that are stable and retain high activity toward substrates when used in substantially water-immiscible organic solvents. The carrageenan-immobilized enzymes are preferably prepared by introducing droplets of an enzyme/carrageenan solution into a chilled alcohol saturated with a potassium salt and hardening the droplets in the alcohol. The alcohol is selected from n-butanol, benzyl alcohol, crotyl alcohol, n-propanol, isopropanol and sec-butanol. Reactions catalyzed by a .kappa.-carrageenan-immobilized esterase include steroselective transesterifications and hydrolysis reactions.
摘要翻译：制备角叉菜胶固定化酶，其在基本上与水不混溶的有机溶剂中使用时是稳定的并保持对底物的高活性。角叉菜胶固定化酶优选通过将酶/角叉菜胶溶液的液滴引入用钾盐饱和的冷冻醇中并使醇中的液滴硬化来制备。醇选自正丁醇，苄醇，巴豆醇，正丙醇，异丙醇和仲丁醇。由κ-卡拉胶来固定的酯酶催化的反应包括固体选择性酯交换反应和水解反应。

2. 发明授权

US4834862A Ampholyte separation method and apparatus 失效
标题翻译：两性电解质分离方法和装置
公开(公告)号：US4834862A
公开(公告)日：1989-05-30
申请号：US243329
申请日：1988-09-12
申请人： Steven J. Breiner , Charles H. Lochmuller
发明人： Steven J. Breiner , Charles H. Lochmuller
IPC分类号： G01N27/447
CPC分类号： G01N27/44795 , G01N27/44747
摘要： An ampholyte separation apparatus and method which utilize a thermally engendered pH gradient to separate amphoteric molecules according to their isoelectric points and/or mobilities. Two independently temperature controllable thermal masses located on opposing ends of a plurality of barriers situated transverse to a flow channel create a pH gradient along the length of the transverse barriers. A pair of electrodes create an electric field along the length of the transverse barriers so as to cause ampholytes traveling down the flow channel to migrate into the transverse barriers and stabilize at points corresponding to their isoelectric points. Alteration of the pH gradient causes the separated amphoteric molecules to reenter the flow stream at different times and to be separately eluted and collected.
摘要翻译：利用热产生的pH梯度根据其等电点和/或迁移率分离两性分子的两性离子分离装置和方法。位于横向于流动通道的多个屏障的相对端上的两个独立的温度可控的热质量沿着横向阻挡层的长度产生pH梯度。一对电极沿着横向阻挡层的长度产生电场，以使两性电解质沿着流动通道向下移动以迁移到横向屏障中并在对应于其等电点的点处稳定。改变pH梯度使得分离的两性分子在不同时间重新进入流动流并被单独洗脱和收集。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式