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    • 1. 发明申请
    • Method for sex biasing spermatozoa
    • US20050114915A1
    • 2005-05-26
    • US10723268
    • 2003-11-26
    • Barb CohenMichael Morris
    • Barb CohenMichael Morris
    • A01K67/027C12N5/00C12N5/071G01N20060101
    • C12N5/0612A01K2227/101A01K2227/105C12N2517/10
    • A method is described for treating a specimen of semen containing sperm cells to increase the relative number of sperm cells of a desired sex type in a treated specimen to increase the potential for conceiving an offspring of the desired sex. A specimen of semen is treated after a predetermined time to increase the relative ability of a at least a portion of the semen to conceive an offspring of the desired sex. The treatment preferably comprises contacting the sperm cells with an agent that preferentially effects sperm cells of a selected sex type. In some preferred embodiments, the semen is separated into two components. A first component has a higher number of sperm of the desired sex type than sperm of a non desired sex type and a second component has a higher number of sperm of the non desired sex type relative to sperm of the desired sex type. In one embodiment, the separating step is performed after a predetermined percent of the sperm cells exhibit a punctate pattern, which is capable of determination by labeling the sperm cells with Koo antibody and determining the percent of cells labeled. In another embodiment, the separating step is performed after waiting for a time determined by locating a maximum in the curve obtained by plotting percent female cells determined by FISH against percent Koo positive cells, determining the time at which the maximum percent female cells occurs, and beginning the following step no earlier than about one hour before the time of the maximum percent female cells. In yet another embodiment, the separating step is performed after waiting for a period of time between about 2 hours and about 24 hours after collection of the ejaculate. Preferably, in the separating step, the sperm is contacted with a cell binding agent, permitting the sperm of the non desired sex type to preferentially bind to the cell binding agent, and the cell binding agent with preferentially bound sperm of the non desired sex type is separated from non bound sperm.
    • 7. 发明申请
    • Method and apparatus for evaluating connective tissue conditions
    • 用于评估结缔组织状况的方法和装置
    • US20070049808A1
    • 2007-03-01
    • US11217755
    • 2005-09-01
    • Blake RoesslerMichael MorrisKaren Dehring
    • Blake RoesslerMichael MorrisKaren Dehring
    • A61B5/00
    • A61B5/0059A61B5/413A61B5/4514A61B5/4528G01N21/35G01N21/359G01N2021/656
    • Methods and apparatus are provided for evaluating a connective tissue condition of a patient (e.g., a disease, a risk of developing a disease, a risk of developing a fracture, etc.). For example, an indicator associated with the connective tissue condition may be generated. First, tissue at a first location of the body of the patient is irradiated using a light source. The tissue may be irradiated in vivo through the skin or via an incision, for example. Alternatively, a biopsy of the tissue may be irradiated. Then, spectral content information for light scattered, reflected, or transmitted by the irradiated tissue is determined. The spectral content information may be used, at least in part, to generate an indicator associated with a condition of connective tissue at a second location of the body of the patient, the second location remote from the first location. The indicator may, for example, assist a physician in diagnosing or ruling out the connective tissue condition.
    • 提供了用于评价患者的结缔组织状况(例如,疾病,发展疾病的风险,发生骨折的风险等)的方法和装置。 例如,可以生成与结缔组织条件相关联的指示符。 首先,使用光源照射患者身体的第一位置处的组织。 组织可以通过皮肤或经由切口照射在体内。 或者,可以照射组织的活组织检查。 然后,确定由照射的组织散射,反射或透射的光的光谱内容信息。 至少部分地,可以使用频谱内容信息来生成与患者身体的第二位置处的结缔组织的状况相关联的指示符,远离第一位置的第二位置。 指示器可以例如帮助医生诊断或排除结缔组织状况。