专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2022017633A2 NOVEL, NON-NATURALLY OCCURRING CRISPR-CAS NUCLEASES FOR GENOME EDITING 审中-公开
公开(公告)号：WO2022017633A2
公开(公告)日：2022-01-27
申请号：PCT/EP2021/000081
申请日：2021-07-20
申请人： BRAIN BIOTECH AG
发明人： SCHOLTZ, Paul , ZUREK, Christian , KROHN, Micahel
IPC分类号： C12N9/22 , C12N15/10 , C12N15/82 , C07K2319/00 , C12N15/102 , C12N15/8213 , C12N15/8257 , C12N2310/20
摘要： The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

2. 发明申请

WO2022017633A8 NOVEL, NON-NATURALLY OCCURRING CRISPR-CAS NUCLEASES FOR GENOME EDITING 审中-公开
公开(公告)号：WO2022017633A8
公开(公告)日：2022-01-27
申请号：PCT/EP2021/000081
申请日：2021-07-20
申请人： BRAIN BIOTECH AG
发明人： SCHOLZ, Paul , ZUREK, Christian , KROHN, Michael
IPC分类号： C12N9/22 , C12N15/10 , C12N15/82 , C07K2319/00 , C12N15/102 , C12N15/8213 , C12N15/8257 , C12N2310/20
摘要： The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

3. 发明申请

WO2022184765A1 NOVEL CRISPR-Cas NUCLEASES FROM METAGENOMES 审中-公开
公开(公告)号：WO2022184765A1
公开(公告)日：2022-09-09
申请号：PCT/EP2022/055257
申请日：2022-03-02
申请人： BRAIN BIOTECH AG
发明人： SCHOLZ, Paul , ZUREK, Christian , BACK, Robin
IPC分类号： C12N15/10 , C12N9/16
摘要： The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of any one of SEQ ID NOs 9, 1 to 5, 7, 8 and 10 to 15; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of any one of SEQ ID NOs 24, 16 to 20, 22, 23 and 25 to 30; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 70% identical to the amino acid sequence of (a), preferably at least 80 % identical, more preferably at least 90% identical, and most preferred at least 95% identical; (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 70% identical to the nucleotide sequence of (b), preferably at least 80 % identical, more preferably at least 90% identical, and most preferred at least 95% identical; (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

4. 发明公开

EP4279597A3 NOVEL, NON-NATURALLY OCCURRING CRISPR-CAS NUCLEASES FOR GENOME EDITING 审中-实审
公开(公告)号：EP4279597A3
公开(公告)日：2024-04-24
申请号：EP23196032.9
申请日：2021-07-20
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian , KROHN, Michael
IPC分类号： C12N9/22 , C12N15/10 , C12N15/82
CPC分类号： C12N9/22 , C12N15/102 , C12N15/8213 , C07K2319/0020130101 , C12N2310/2020170501 , C12N15/8257
摘要： The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

5. 发明公开

EP4279597A2 NOVEL, NON-NATURALLY OCCURRING CRISPR-CAS NUCLEASES FOR GENOME EDITING 审中-实审
公开(公告)号：EP4279597A2
公开(公告)日：2023-11-22
申请号：EP23196032.9
申请日：2021-07-20
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian , KROHN, Michael
IPC分类号： C12N15/82
摘要： The present invention relates to a nucleic acid molecule encoding an RNA-guided DNA endonuclease, which is (a) a nucleic acid molecule encoding the RNA-guided DNA endonuclease comprising or consisting of the amino acid sequence of SEQ ID NO: 29, 1 or 3; (b) a nucleic acid molecule comprising or consisting of the nucleotide sequence of SEQ ID NO: 30, 2 or 4; (c) a nucleic acid molecule encoding a RNA-guided DNA endonuclease the amino acid sequence of which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the amino acid sequence of (a); (d) a nucleic acid molecule comprising or consisting of a nucleotide sequence which is at least 90 %, preferably at least 92 %, and most preferably at least 95 % identical to the nucleotide sequence of (b); (e) a nucleic acid molecule which is degenerate with respect to the nucleic acid molecule of (d); or (f) a nucleic acid molecule corresponding to the nucleic acid molecule of any one of (a) to (d) wherein T is replaced by U.

6. 发明公开

EP4025691A2 NOVEL, NON-NATURALLY OCCURRING CRISPR-CAS NUCLEASES FOR GENOME EDITING 有权
公开(公告)号：EP4025691A2
公开(公告)日：2022-07-13
申请号：EP21765836.8
申请日：2021-07-20
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian , KROHN, Michael
IPC分类号： C12N9/22 , C12N15/10 , C12N15/82

7. 发明公开

EP4361265A1 OPTIMIZATION OF EDITING EFFICACY OF CRISPR NUCLEASES WITH COLLATERAL ACTIVITY 审中-公开
公开(公告)号：EP4361265A1
公开(公告)日：2024-05-01
申请号：EP22204062.8
申请日：2022-10-27
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian
IPC分类号： C12N15/10 , C12N9/22 , C12N15/90
CPC分类号： C12N15/102 , C12N2310/2020170501 , C12N9/22 , C12N15/902 , C12N15/815 , C12N15/70
摘要： The present invention relates to a method for the genome editing of cells at a target locus by homology directed repair (HDR) within a cell population and concurrently enriching within said cell population the HDR-genome edited cells, wherein the method comprises (A) introducing into the cells within the cell population one or more nucleic acid molecules, said one or more nucleic acid molecules encoding in expressible form (i) a CRISPR nuclease comprising or consisting of (a) an amino acid sequence of any one of SEQ ID NO: 1, 2 or 3; (b) an amino acid sequence being encoded by the nucleotide sequence of SEQ ID NO: 4, 5, 6 or 7; (c) an amino acid sequence being at least 80% identical to the amino acid sequence of (a), or (d) an amino acid sequence being encoded by a nucleic acid sequence being at least 80 % identical to the nucleotide sequence of (b), (ii) a guide RNA comprising (a) a first segment comprising a nucleotide sequence that is complementary to a sequence at the target locus and (b) a second segment that interacts with the CRISPR nuclease of (i), wherein the CRISPR nuclease and/or the guide RNA are encoded by said one or more nucleic acid molecules in a form wherein the expression can be induced or repressed, and a nucleic acid molecule comprising, consisting of, or encoding (iii) a donor template with homology to the target locus; and (B) culturing the cells at least for a time wherein the cells double under growth conditions wherein the target locus within the cells of the cell population becomes modified by homology directed repair (HDR), and (C) inducing after step (B) or repressing in steps (A) and (B) the expression of the CRISPR nuclease and/or the guide RNA within the cells thereby enriching within said cell population the HDR-genome edited cells.

8. 发明公开

EP4301852A1 NOVEL CRISPR-CAS NUCLEASES FROM METAGENOMES 审中-实审
公开(公告)号：EP4301852A1
公开(公告)日：2024-01-10
申请号：EP22707784.9
申请日：2022-03-02
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian , BACK, Robin
IPC分类号： C12N15/10 , C12N9/16

9. 发明公开

EP4215611A1 MODIFICATION OF THE GENOME OF A FILAMENTOUS FUNGUS 审中-实审
公开(公告)号：EP4215611A1
公开(公告)日：2023-07-26
申请号：EP22152247.7
申请日：2022-01-19
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , ZUREK, Christian , HEGER, Rebecca, Marie
IPC分类号： C12N15/10 , C12N9/22 , C12N15/63 , C12N15/80
摘要： The present invention relates to a method for modifying a nucleotide sequence at a target site in the genome of the cell of a filamentous fungus comprising introducing into said cell one or more nucleic molecules comprising a first and a second expression cassette, wherein the first expression cassette comprises a nucleic acid molecule encoding a class 2, type V RNA-guided DNA endonuclease; and wherein the second expression cassette comprises a nucleic acid molecule encoding a DNA-targeting and self-splicing RNA comprising from the 5'-end to the 3'-end (a) a first self-splicing sequence, (b) a stem loop sequence of SEQ ID NO: 7 or 8 or a sequence being at least 75% identical thereto, (c) a nucleotide sequence that is complementary to a sequence at the target site; and (d) a second self-splicing sequence.

10. 发明公开

EP4118196A1 CRISPR-CAS NUCLEASES FROM CPR-ENRICHED METAGENOME 无效
公开(公告)号：EP4118196A1
公开(公告)日：2023-01-18
申请号：EP21715132.3
申请日：2021-03-11
申请人： BRAIN Biotech AG
发明人： SCHOLZ, Paul , PUL, Uemit , ZUREK, Christian , HIEI, Yukoh , YANAGIHARA, Chizu , TSUKAMOTO, Hiroshi , KOMARI, Toshihiko
IPC分类号： C12N9/22 , C12N15/55 , C12N15/10 , C12N15/82

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式