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1. 发明专利

JPH11196899A DETERMINATION OF CREATINE KINASE AND REAGENT THEREFOR 失效
公开(公告)号：JPH11196899A
公开(公告)日：1999-07-27
申请号：JP31377298
申请日：1998-11-04
申请人： BOEHRINGER MANNHEIM GMBH
发明人： SIEDEL JOACHIM , NAGEL ROLF
IPC分类号： C12Q1/32 , C12Q1/50
摘要： PROBLEM TO BE SOLVED: To determine the subject activity by using a stable reagent by subjecting ATP produced from a substrate and ADP to phosphorylation with hexose and detecting the ATP with a hexose-6-phosphatedehydrogenase, or the like. SOLUTION: In this method for enzymatically measuring creatine kinase in a biological sample substance by producing ATP from creatine phosphate and ADP and detecting the produced ATP by using glucose, hexokinase, hexose-6-phosphate dehydrogenase and NAD or NADP , hexose different from glucose and capable of enzymatically being phosphorylated is added to glucose, or hexose-6-phosphoric acid used as a substrate for hexokinase instead of the hexose and produced by hexokinase reaction is directly measured by using hexose-6-phosphate dehydrogenase or measured as glucose-6-phosphoric acid by glucose-6-phosphate dehydrogenase after enzymatic isomerization.

2. 发明专利

JPS58175488A STABILIZATION OF AQUEOUS SOLUTION OF CHOLESTERINE ESTERASE FROM PSEUDOMONAS STRAIN 失效
公开(公告)号：JPS58175488A
公开(公告)日：1983-10-14
申请号：JP56883
申请日：1983-01-07
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BUSCHEK HERBERT , SCHLUMBERGER HELMUT , SIEDEL JOACHIM
IPC分类号： C12N20060101 , C12N9/16 , C12N9/18 , C12N9/96 , C12Q20060101 , C12Q1/44 , C12Q1/46 , C12Q1/60 , C12Q1/61 , C12R1/38 , C12R1/39

3. 发明专利

DK163527C 未知
公开(公告)号：DK163527C
公开(公告)日：1992-08-03
申请号：DK283
申请日：1983-01-03
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BUSCHEK HERBERT , SCHLUMBERGER HELMUT , SIEDEL JOACHIM
IPC分类号： C12N20060101 , C12N9/16 , C12N9/18 , C12N9/96 , C12Q20060101 , C12Q1/44 , C12Q1/46 , C12Q1/60 , C12Q1/61 , C12R1/38 , C12R1/39
摘要： The present invention provides a process for the stabilization of an aqueous solution of cholesterol esterase from Pseudomonas, especially in the presence of a surface-active agent, wherein the enzyme is dissolved in a phosphate-free buffer which contains 10 to 200 mMol/liter of magnesium ions.

4. 发明专利

FI83975C FOERFARANDE OCH REAGENS FOER SPECIFIK BESTAEMNING AV HDL-KOLESTEROLHALT I SERUM. 未知
公开(公告)号：FI83975C
公开(公告)日：1991-09-25
申请号：FI863752
申请日：1986-09-17
申请人： BOEHRINGER MANNHEIM GMBH
发明人： KERSCHER LORENZ , SIEDEL JOACHIM , ZIEGENHORN JOACHIM , PAUTZ BRIGITTE
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92

5. 发明专利

FI83975B 未知
公开(公告)号：FI83975B
公开(公告)日：1991-06-14
申请号：FI863752
申请日：1986-09-17
申请人： BOEHRINGER MANNHEIM GMBH
发明人： KERSCHER LORENZ , SIEDEL JOACHIM , ZIEGENHORN JOACHIM , PAUTZ BRIGITTE
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92
摘要： The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement. The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains 0.1 to 10 U/ml. cholesterol esterase 0.05 to 10 U/ml. cholesterol oxidase 20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0) 0.2 to 20 mmole/liter of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate and, separately therefrom, 0.02 to 2% non-ionic polyethylene oxide group-containing detergent or secondary alkane sulphonate, and optionally 0.05 to 2% of an alcohol containing up to 3 carbon atoms.

6. 发明专利

DK157771B 未知
公开(公告)号：DK157771B
公开(公告)日：1990-02-12
申请号：DK324384
申请日：1984-07-02
申请人： BOEHRINGER MANNHEIM GMBH
发明人： SIEDEL JOACHIM , STAEPELS JOHNNY , ZIEGENHORN JOACHIM
IPC分类号： C09K23/02 , C09K23/34 , G01N33/53 , G01N33/48 , G01N33/536 , G01N33/92 , G01N33/96
摘要： The present invention provides an agent for the removal of a turbidity in a biological fluid, wherein it contains (a) a polyethoxylated triglyceride with an HLB value of 4 to 14, (b) a secondary n-alkane sulphonate, as well as optionally (c) a futher non- or anionic tenside, in aqueous, optionally buffered solution.

7. 发明专利

AU574931B2 SPECIFIC DETERMINATION OF HDL CHOLESTEROL IN SERUM 未知
公开(公告)号：AU574931B2
公开(公告)日：1988-07-14
申请号：AU6116386
申请日：1986-08-14
申请人： BOEHRINGER MANNHEIM GMBH
发明人： KERSCHER LORENZ , SIEDEL JOACHIM , ZIEGENHORN JOACHIM , PAUTZ BRIGITTE
IPC分类号： C12Q1/00 , C12Q1/26 , C12Q1/44 , C12Q1/60 , G01N33/92 , C12Q1/46
摘要： The present invention provides a process for the specific determination of HDL cholesterol in serum or plasma by incubation with a cholesterol detection system, containing cholesterol oxidase and cholesterol esterase in a buffered aqueous medium, and measurement of a product of the cholesterol oxidase reaction or of the oxygen consumption, wherein a sample to be tested is incubated in the presence of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate, a first measurement is then carried out, subsequently a non-ionic, polyethylene oxide group-containing detergent or a secondary alkane sulphonate is added, again incubated and a second measurement is carried out, the amount of HDL cholesterol being determined from the difference between the first and second measurement. The present invention also provides a reagent for the specific determination of HDL cholesterol in serum or plasma containing cholesterol oxidase, buffer and cholesterol esterase, wherein, referred to the final solution, it contains 0.1 to 10 U/ml. cholesterol esterase 0.05 to 10 U/ml. cholesterol oxidase 20 to 500 mmole/liter buffer substance (pH 6.0 to 8.0) 0.2 to 20 mmole/liter of a salt of a bile acid or of a bile acid derivative or of dioctylsulphosuccinate and, separately therefrom, 0.02 to 2% non-ionic polyethylene oxide group-containing detergent or secondary alkane sulphonate, and optionally 0.05 to 2% of an alcohol containing up to 3 carbon atoms.

8. 发明专利

DE3561954D1 PROCESS AND REAGENT FOR TOTAL ENZYMATIC ANALYSIS OF UREA 未知
公开(公告)号：DE3561954D1
公开(公告)日：1988-04-28
申请号：DE3561954
申请日：1985-05-23
申请人： BOEHRINGER MANNHEIM GMBH
发明人： SIEDEL JOACHIM , WAHLEFELD AUGUST , ZIEGENHORN JOACHIM
IPC分类号： G01N33/62 , C12N9/02 , C12N9/04 , C12N9/88 , C12Q1/56 , C12Q1/58
摘要： PCT No. PCT/EP85/00250 Sec. 371 Date Jan. 10, 1986 Sec. 102(e) Date Jan. 10, 1986 PCT Filed May 23, 1985 PCT Pub. No. WO85/05641 PCT Pub. Date Dec. 19, 1985.For the enzymatic determination of urea, one reacts the urea with glyoxylate in the presence of ureidoglycolate synthetase to give (S)-ureidoglycolate and oxidizes the latter with NAD(P)+ and ureidoglycolate dehydrogenase to carbamoyloxamate and measures NAD(P)H formed either directly or via a color indicator system. A reagent suitable herefor contains glyoxylate, NAD(P)+, (S)-ureidoglycolate synthetase, ureidoglycolate dehydrogenase and buffer substance.

9. 发明专利

AT33150T VERFAHREN UND REAGENZ ZUR VOLLENZYMATISCHEN BESTIMMUNG VON HARNSTOFF. 未知
公开(公告)号：AT33150T
公开(公告)日：1988-04-15
申请号：AT85902535
申请日：1985-05-23
申请人： BOEHRINGER MANNHEIM GMBH
发明人： SIEDEL JOACHIM , WAHLEFELD AUGUST WILHELM , ZIEGENHORN JOACHIM
IPC分类号： C12N9/02 , C12N9/88 , C12Q1/58

10. 发明专利

NO875418L 未知
公开(公告)号：NO875418L
公开(公告)日：1987-12-23
申请号：NO875418
申请日：1987-12-23
申请人： BOEHRINGER MANNHEIM GMBH
发明人： DELANGHE JORIS R , BUYZERE MARC L DE , SCHEERDER IVAN K DE , SIEDEL JOACHIM , STAEPELS JOHNNY
IPC分类号： C12Q1/00

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