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1. 发明授权

US3862009A Determination of triglycerides 失效
标题翻译： TRIGLYCERIDES的测定
公开(公告)号：US3862009A
公开(公告)日：1975-01-21
申请号：US36535573
申请日：1973-05-30
申请人： BOEHRINGER MANNHEIM GMBH
发明人： WAHLEFELD AUGUST WILHELM , MOLLERING HANS , GRUBER WOLFGANG , BERNT ERICH , ROESCHLAU PETER
IPC分类号： G01N33/92 , C12Q1/02 , C12Q1/44 , C12Q1/48 , C12Q1/61 , G01N33/53 , C07G7/02 , C12K1/00
CPC分类号： C12Q1/48 , C12Q1/44 , C12Q1/61
摘要： Triglycerides are determined by enzymatic saponification with lipase and measurement of the liberated glycerol by carrying out the saponification in the presence of carboxylesterase and an alkali metal or alkaline earth metal alkyl sulfate; reagents are provided for carrying out the process and comprise a buffer and, as a saponification agent, lipase, carboxylesterase and the alkali metal or alkaline earth metal alkyl sulfate, and a glycerol detection system.
摘要翻译：通过脂肪酶的酶促皂化来测定甘油三酸酯，并通过在羧酸酯酶和碱金属或碱土金属烷基硫酸盐的存在下进行皂化来测定游离的甘油。提供了用于进行该方法的试剂，并且包含缓冲剂和作为皂化剂的脂肪酶，羧酸酯酶和碱金属或碱土金属烷基硫酸盐以及甘油检测系统。

2. 发明授权

US3925164A Method for the determination of cholesterol 失效
标题翻译：方法确定胆固醇
公开(公告)号：US3925164A
公开(公告)日：1975-12-09
申请号：US45452274
申请日：1974-03-25
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEAUCAMP KLAUS , MOLLERING HANS , LANG GUNTER , GRUBER WOLFGANG , ROESCHLAU PETER
IPC分类号： C12N9/18 , C12Q1/60 , C12K1/04
CPC分类号： C12N9/18 , C12Q1/60 , Y10S435/827 , Y10S435/886 , Y10S435/893 , Y10S435/911 , Y10S435/913 , Y10S435/921 , Y10S435/922 , Y10S435/933
摘要： Total cholesterol or bound cholesterol in a sample is determined by treating the sample with cholesterol esterase, thereby releasing the bound cholesterol, and then determining the resulting total cholesterol by known methods; specifically preferred are cholesterol esterases from Candida rugosa ATCC 14830, Rhizopus spec. WS 90027 and Aspergillus spec. WS 90030.

3. 发明授权

US3920400A Uric acid standard solution 失效
标题翻译：尿酸标准溶液
公开(公告)号：US3920400A
公开(公告)日：1975-11-18
申请号：US43981974
申请日：1974-02-05
申请人： BOEHRINGER MANNHEIM GMBH
发明人： SCHEIBE PETER , BERNT ERICH , GRUBER WOLFGANG
IPC分类号： G01N33/50 , C12Q1/62 , G01N33/96 , G01N31/06 , C09K15/06 , C09K15/12 , C09K15/20
CPC分类号： G01N33/96 , C12Q1/62 , Y10S424/06 , Y10T436/103332 , Y10T436/108331 , Y10T436/148888
摘要： Uric acid standard composition comprising an aqueous buffered solution of uric acid in the form of a lithium salt thereof, with a pH value of between 6 and 8, and at least one complexing agent for the higher valence stage of polyvalent heavy metal ions and/or an alkali azide, are remarkably stable and useful in the quantitative determination of uric acid in enzymatic and chemical processes.
摘要翻译：尿酸标准物组合物包含其pH值为6至8的锂盐形式的尿酸水溶液缓冲溶液和至少一种用于多价重金属离子的较高价态阶段的络合剂和/或碱性叠氮化物在酶和化学过程中的尿酸定量测定中是非常稳定和有用的。

4. 发明专利

HRP940728B1 PROCESS FOR THE PREPARATION OF OLYGOGLUCOSIDE DERIVATIVES 未知
公开(公告)号：HRP940728B1
公开(公告)日：1996-06-30
申请号：HRP940728
申请日：1994-10-21
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , SCHAICH EUGEN , NEUMANN ULRICH , WAHLEFELD AUGUST , GRUBER WOLFGANG , EMPL BERNARD
IPC分类号： C07H3/06 , C07H9/02 , C07H15/203 , C12Q1/40

5. 发明专利

SI8411385A8 PROCESS FOR PREPARATION OF OLIGOGLUCOSIDE DERIVATIVES 未知
公开(公告)号：SI8411385A8
公开(公告)日：1996-02-29
申请号：SI8411385
申请日：1984-08-07
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , SCHAICH EUGEN , NEUMANN ULRICH , WAHLEFELD AUGUST , GRUBER WOLFGANG , EMPL BERNHARD
IPC分类号： C07H3/06 , C07H9/02 , C07H15/203 , C12Q1/40

6. 发明专利

CA1240990A HEPTAOSE COMPOUNDS 未知
公开(公告)号：CA1240990A
公开(公告)日：1988-08-23
申请号：CA460232
申请日：1984-08-02
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , SCHAICH EUGEN , NEUMANN ULRICH , WAHLEFELD AUGUST , GRUBER WOLFGANG , EMPL BERNHARD
IPC分类号： G01N33/50 , C07H3/06 , C07H9/02 , C07H15/04 , C07H15/203 , C07H21/00 , C08B37/00 , C12Q1/40 , C07H15/20
摘要： Heptaose compounds of the general formula: (I) in which R and R1, independently of one another, each represent a straight-chained or branched alkyl or alkanoyl radical containing up to 6 carbon atoms or a phenyl radical or R and R1 together also form a methylene bridge, the hydrogen atoms of which, independently of one another, can each be substituted by an alkyl radical containing up to 5 carbon atoms or a phenyl radical, R2 represents an oligoglucoside residue containing 2 to 7 glucose units and X is a hydrogen atom or an optically-determinable residue; compounds (I) are useful as substrates for the determination of .alpha.-amylase which is important in clinical testing of the pancreas function.

7. 发明专利

DK151396B 未知
公开(公告)号：DK151396B
公开(公告)日：1987-11-30
申请号：DK172374
申请日：1974-03-28
申请人： BOEHRINGER MANNHEIM GMBH
发明人： BEAUCAMP KLAUS , MOLLERING HANS , LANG GUNTER , GRUBER WOLFGANG , ROESCHLAU PETER
IPC分类号： C12N9/18 , C12Q1/60 , G01N33/16 , C12Q1/00
摘要： 1429526 Cholesterol esterase BOEHRINGER MANNHEIM GmbH 25 March 1974 [28 March 1973 3 April 1973] 13159/74 Heading C3H [Also in Division G1] Cholesterol esterase-containing materials are obtained from Candida rugosa ATCC 14830, Aspergillus spec. WS 90030 and the following micro-organisms: Actinomyces aureoverticillium WS 90002, Actinomyces cyaneofuscatus WS 90003, Actinomyces griseomycini WS 90004, Actinomyces longisporus-fl. WS 90005, Actinomyces malachiticus WS 90006, Actinomyces roseolus WS 90007, Actinomyces toxytricini WS 90008, Actinomyces variabilis Streptomyces spec. WS 90010, Streptomyces autotrophicus WS 90011, Streptomyces canescens WS 90012, Streptomyces chartreusis WS 90013, Streptomyces michiganensis WS 90014, Streptomyces murinus WS 90015, Streptomyces hachijoensis WS 90016, Streptomyces caelestes WS 90017, Streptomyces tendae WS 90018, Nocardia rubra WS 90019, Candida mycoderma WS 90020, Candida albicans WS 90021, Candida albicans WS 90022, Candida albicans WS 90023, Candida spec. WS 90024, Cunninghamella elegans WS 90025, Mucor mucedo WS 90026, Shizopus spec. WS 90027, Penicillium spec. WS 90028 and Aspergillus spec. WS 90029. The micro-organisms may be used as such or in digested form, e.g. as an acetonedried powder as sources of cholesterol esterase. The micro-organisms are preferably cultured in nutrient media containing cholesterol esters, multistage culturing being particularly suitable.

8. 发明专利

FI72145B 未知
公开(公告)号：FI72145B
公开(公告)日：1986-12-31
申请号：FI800577
申请日：1980-02-27
申请人： BOEHRINGER MANNHEIM GMBH
发明人： GRUBER WOLFGANG , LENZ HELMUT , LOOSER SIEGFRIED , LINKE HANS-RALF
IPC分类号： C12N9/12 , A61K39/00 , C12Q1/50 , G01N33/573 , G01N33/70
摘要： A method for the determination of creatine kinase-MB in serum by immunological exclusion of sub-unit M and measurement of the sub-unit B for the determination of creatine kinase, the improvement comprising, for the purpose of excluding the sub-unit M, adding to the reaction mixture comprising determination reagents and the creatine kinase-containing sample, monovalent fragments obtained from antibodies against the sub-unit M by proteolytic cleavage under reducing conditions.

9. 发明专利

FI69641B 未知
公开(公告)号：FI69641B
公开(公告)日：1985-11-29
申请号：FI800578
申请日：1980-02-27
申请人： BOEHRINGER MANNHEIM GMBH
发明人： WULFF KARL , STAEHLER FRITZ , GRUBER WOLFGANG
IPC分类号： C12Q1/42 , C12Q1/50 , C12Q1/66
摘要： Creatine kinase is determined by the reaction of creatine phosphate with adenosine diphosphate with the formation of adenosine triphosphate, reaction of the latter with luciferin and oxygen in the presence of luciferase and diadenosine pentaphosphate with the formation of oxyluciferin and adenosine monophosphate, and measurement of the light thereupon emitted, the reaction being performed with a saturated concentration of adenosine diphosphate and substrate, in the presence of 1 to 10 millimoles per liter of AMP at pH values of 5.8 to 7.5, with at least 50 units of luciferase per test. It is desirable to operate in the presence of diadenosine pentaphosphate or sodium fluoride and a sequestering agent.

10. 发明专利

FI68418B 未知
公开(公告)号：FI68418B
公开(公告)日：1985-05-31
申请号：FI820586
申请日：1982-02-23
申请人： BOEHRINGER MANNHEIM GMBH
发明人： RAUSCHER ELLI , NEUMANN ULRICH , WAHLEFELD AUGUST WILHELM , HAGEN ALEXANDER , GRUBER WOLFGANG , ZIEGENHORN JOACHIM , SCHAICH EUGEN , DENEKE ULFERT , MICHAL GERHARD , WEIMANN GUENTER
IPC分类号： C07H20060101 , C07H15/00 , C12P19/18 , C12P19/44

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