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1. 发明申请

WO2003031973A1 LDL RECEPTOR SIGNALING ASSAYS 审中-公开
标题翻译：低密度脂蛋白受体信号测定
公开(公告)号：WO2003031973A1
公开(公告)日：2003-04-17
申请号：PCT/US2002/032271
申请日：2002-10-10
申请人： BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM
发明人： HERZ, Joachim , MAY, Petra
IPC分类号： G01N33/53
CPC分类号： C12Q1/37 , G01N33/92
摘要： The invention provides methods and compositions for modeling and detecting LDL receptor transmembrane signaling by detecting proteolysis of an LDL receptor transmembrane domain. The method comprises the steps of: a) providing a sample comprising a cell membrane comprising (I) a polypeptide comprising an LDL receptor transmembrane domain fused to a C-terminal tail, and (ii) a protease which specifically cleaves the domain and thereby releases the tail from the membrane; b) incubating the sample under conditions wherein the protease cleaves the domain and thereby releases the tail from the membrane; and c) detecting a resultant released tail.
摘要翻译：本发明提供了通过检测LDL受体跨膜结构域的蛋白水解来建模和检测LDL受体跨膜信号的方法和组合物。该方法包括以下步骤：a）提供包含细胞膜的样品，所述样品包含（I）包含与C末端尾部融合的LDL受体跨膜结构域的多肽，和（ii）特异性切割结构域并由此释放的蛋白酶从膜的尾巴; b）在其中蛋白酶切割结构域并从而从膜释放尾部的条件下孵育样品; 和c）检测产生的释放尾巴。

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