专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

2. 发明申请

WO2004027040A3 DEVICES AND METHODS FOR ISOLATING AND RECOVERING TARGET CELLS 审中-公开
标题翻译：用于分离和回收靶细胞的装置和方法
公开(公告)号：WO2004027040A3
公开(公告)日：2004-09-02
申请号：PCT/US0329794
申请日：2003-09-17
申请人： MRDX TECHNOLOGIES INC , ROSS AMELIA A , BERNSTEIN STEVE
发明人： ROSS AMELIA A , BERNSTEIN STEVE
IPC分类号： C12M1/34 , C12N5/00 , C12N5/06 , G01N33/483 , G01N33/569 , C07K16/28
CPC分类号： C12M23/46 , C12M47/04 , G01N33/56966
摘要： A cell isolating device and method is provided to concentrate or isolate cells with specific characteristics from a mixture of different cell types. One embodiment may comprise two subtypes of antibodies that are directly conjugated to biotin (Abb) and conjugated to a fluorescent molecule (Abf). The conjugated antibodies (Abb +Abf) bind to the target cells in a mixed cell suspension. The cell suspension is then passed over an immobilized avidin or streptavidin substrate on a glass microscope slide. The biotinylated target cells adhere to the avidin/streptavidin substrate, while the unbound cells are washed off and collected in a wicking member. Captured cells on the avid in/streptavidin substrate may then be visualized directly using a fluorescent microscope or detected and enumerated via an on-board fluorescent detection device. Additional chemicals and/or physical manipulation may then be applied to the device to release viable target cells for subsequent analysis.
摘要翻译：提供细胞分离装置和方法以从不同细胞类型的混合物中浓缩或分离具有特定特征的细胞。一个实施方案可以包含与生物素（Abb）直接缀合并与荧光分子（Abf）缀合的两种亚型的抗体。缀合抗体（Abb + Abf）在混合细胞悬浮液中与靶细胞结合。然后将细胞悬液在玻璃载玻片上通过固定的抗生物素蛋白或抗生蛋白链菌素底物。生物素化的靶细胞粘附于抗生物素蛋白/抗生蛋白链菌素底物，而未结合的细胞被洗掉并收集在芯吸构件中。然后可以使用荧光显微镜直接观察在抗生蛋白链菌素底物中捕获的细胞，或者通过板上荧光检测装置检测和列举。然后可以对装置施加额外的化学物质和/或物理操作以释放活的靶细胞用于随后的分析。

IPRDB

热门服务

关于我们

友情链接

联系方式