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1. 发明申请

WO2015172134A1 COSMIC QUENCHERS 审中-公开
公开(公告)号：WO2015172134A1
公开(公告)日：2015-11-12
申请号：PCT/US2015/030097
申请日：2015-05-11
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： REDDINGTON, Mark , COOK, Ronald, M. , SOWERS, Ben
IPC分类号： C07D221/02 , C07D221/06
CPC分类号： C07D471/06 , C07D455/04 , C07F9/6561 , C07H17/02 , C07H21/00 , C12Q1/6818
摘要： The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.
摘要翻译：本发明提供了包含这些猝灭剂的激发态能量猝灭剂，探针和其它共轭物及其使用方法。

2. 发明申请

WO2017048982A1 OLIGOPHOSPHATE DARK QUENCHERS AND USES 审中-公开
标题翻译：低磷酸盐低密度猝灭剂和用途
公开(公告)号：WO2017048982A1
公开(公告)日：2017-03-23
申请号：PCT/US2016/051976
申请日：2016-09-15
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： DEMPCY, Robert , WONG, Albert , JOHANSSON, Hans , COOK, Ronald, M.
IPC分类号： C12Q1/68 , C07F9/24
CPC分类号： C07F9/65583 , C07F9/6561 , C12Q1/68 , C12Q1/6818 , C12Q2525/117 , C12Q2565/1015
摘要： The present invention provides quenchers of excited state energy, probes and other conjugates comprising these quenchers, and methods of their use.
摘要翻译：本发明提供了包含这些猝灭剂的激发态能量猝灭剂，探针和其它共轭物及其使用方法。

3. 发明申请

WO2006010038A2 INDUCIBLE FLUORESCENCE ASSAY 审中-公开
标题翻译：可溶性荧光测定
公开(公告)号：WO2006010038A2
公开(公告)日：2006-01-26
申请号：PCT/US2005/024341
申请日：2005-07-08
申请人： BIOSEARCH TECHNOLOGIES, INC. , COOK, Ronald, M. , SAXON ARMSTRONG, Eliana , JOHANSSON, Hans, E.
发明人： COOK, Ronald, M. , SAXON ARMSTRONG, Eliana , JOHANSSON, Hans, E.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/26 , C09B11/08 , C12Q1/6818 , C12Q1/6897 , G01N33/542 , G01N2333/90209 , Y10S436/903 , Y10T436/13
摘要： In one aspect, the invention provides fluorescent probes and assays. The probes include a fluorophore-quencher pair that undergoes a switch from dark to fluorescent in response to a reaction of the quencher. The switch of the probe from dark to fluorescent is typically mediated by an enzyme that acts directly or indirectly on the quencher, interfering with its ability to quench fluorescence emission from the fluorophore. In another aspect, the invention provides a reporter gene assay system and methods of using this system. The assay system includes a fluorophore-quencher probe and an enzyme that acts directly or indirectly on the quencher, increasing the fluorescent emission of the fluorophore. In still other aspects, the invention provides nucleic acid constructs and cells expressing the peptide products of these constructs. In assays of the invention, the presence of a target substance is detected by the switching of fluorescence mediated by the change in oxidation state of the quencher. The assay systems are of use in numerous assay formats, e.g., confirming the expression of the enzyme that acts on the quencher, and/or detecting a species conjugated to the enzyme.
摘要翻译：一方面，本发明提供荧光探针和测定法。探针包括响应于猝灭剂的反应经历从暗变为荧光的荧光团 - 猝灭剂对。探针从黑暗到荧光的切换通常由直接或间接作用于猝灭剂的酶介导，干扰其从荧光团中淬灭荧光发射的能力。另一方面，本发明提供报道基因测定系统和使用该系统的方法。测定系统包括荧光团猝灭剂探针和直接或间接作用于猝灭剂的酶，增加荧光团的荧光发射。在其它方面，本发明提供了表达这些构建体的肽产物的核酸构建体和细胞。在本发明的测定中，通过由猝灭剂的氧化态的变化介导的荧光的转换来检测目标物质的存在。测定系统可用于许多测定形式，例如确认作用于猝灭剂的酶的表达和/或检测与酶缀合的物质。

4. 发明申请

WO2016168750A1 DUAL QUENCHER PROBES 审中-公开
标题翻译：双重探测器探测器
公开(公告)号：WO2016168750A1
公开(公告)日：2016-10-20
申请号：PCT/US2016/027964
申请日：2016-04-15
申请人： BIOSEARCH TECHNOLOGIES, INC. , COOK, Ronald, M. , SOWERS, Ben , BEAL, Marc, P.
发明人： COOK, Ronald, M. , SOWERS, Ben , BEAL, Marc, P.
IPC分类号： C07H19/04 , C07D241/46 , C07F9/09
CPC分类号： C12Q1/6818 , C07D241/46 , C07D311/82 , C07H15/18 , C07H19/06 , C07H21/00
摘要： The present invention provides nucleic acid probes comprising two quenchers of excited state energy, and methods of their use.
摘要翻译：本发明提供了包含两个激发态能量猝灭剂的核酸探针及其使用方法。

5. 发明申请

WO2009124150A3 STABILIZED NUCLEIC ACID DARK QUENCHER-FLUOROPHORE PROBES 审中-公开
公开(公告)号：WO2009124150A3
公开(公告)日：2009-10-08
申请号：PCT/US2009/039213
申请日：2009-04-01
申请人： BIOSEARCH TECHNOLOGIES, INC. , COOK, Ronald, M. , LYTTLE, Matt
发明人： COOK, Ronald, M. , LYTTLE, Matt
IPC分类号： C12Q1/68 , C07H21/04
摘要： The present invention provides a new class of solids supports for synthesis of modified oligomers of nucleic acids, and nucleic acid probes that have a format expediently synthesized on the new supports. Exemplary solid supports include at least one quencher bound through a linker to the solid support. Various exemplary embodiments include a moiety that stabilizes a duplex, triplex or higher order aggregation (e.g., hybridization) of nucleic acids of which the oligomer of the invention is a component. Other components of the solid support include moieties that stabilize aggregations of nucleic acids, e.g., intercalators, minor groove binding moieties, bases modified with a stabilizing moiety (e.g., alkynyl moieties, and fluoroalkyl moieties), and conformational stabilizing moieties, such as those described in commonly owned U.S. Patent Application Publication No. 2007/0059752.

6. 发明申请

WO2003049852A1 MULTI-CHANNEL REAGENT DISPENSING APPARATUS AND METHOD 审中-公开
标题翻译：多通道试剂分配装置和方法
公开(公告)号：WO2003049852A1
公开(公告)日：2003-06-19
申请号：PCT/US2002/038872
申请日：2002-12-05
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： COOK, Ronald, M. , DILL, Rand , BALTZLEY, Arnie
IPC分类号： B01J19/00
CPC分类号： G01N35/1002 , B01J19/0046 , B01J2219/00315 , B01J2219/00317 , B01J2219/0036 , B01J2219/00389 , B01J2219/00414 , B01J2219/00423 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00689 , B01J2219/00722 , B01L3/0203 , C40B40/06 , C40B60/14 , G01N35/1065 , Y10T436/11 , Y10T436/114165 , Y10T436/119163 , Y10T436/2575
摘要： Embodiments of the present invention are directed to an improved chemical synthesis apparatus for performing chemical synthesis such as nuclei acid synthesis in a plurality of reaction wells or cells in an efficient manner. The apparatus employs dispenser heads (16) that each include a cluster of nozzles (14) which are fluidicly coupled to a plurality of reagent sources (12) for dispensing different reagents through the single dispenser head. Because each dispenser head (16) is capable of dispensing a plurality of different reagents, the apparatus can perform simultaneous synthesis in a plurality of cells at a high throughput without complex and elaborate control of movement of the dispenser heads (16) relative to the cells.
摘要翻译：本发明的实施方案涉及一种用于在多个反应孔或细胞中以有效的方式进行化学合成如核酸合成的改进的化学合成装置。该装置使用分配器头16，其分别包括一组喷嘴14，喷嘴14流体地联接到多个试剂源12，用于通过单个分配头分配不同的试剂。因为每个分配器头部16能够分配多个不同的试剂，所以该设备可以在高通量下在多个单元中执行同时合成，而无需对分配头16相对于单元的运动进行复杂而精细的控制。

7. 发明申请

WO2015167620A1 COMPOUNDS COMPOSITIONS AND METHODS INCLUDING THERMALLY LABILE MOIETIES 审中-公开
标题翻译：化合物组合物和方法，包括热可溶性动物
公开(公告)号：WO2015167620A1
公开(公告)日：2015-11-05
申请号：PCT/US2015/000046
申请日：2015-04-15
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： COOK, Ronald, M. , COOK, Brett, M. , LYTTLE, Matthew, H.
IPC分类号： C07H19/16
CPC分类号： A61K41/0042 , A61K31/7068 , A61K31/7072 , A61K31/7076 , A61K31/708 , B01J19/24 , B01J2219/00051 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07H21/00 , C12N15/113 , C12N2310/314 , C12N2330/30 , C12P19/34 , Y02P20/55
摘要： The present invention generally relates to compounds that include one or more thermally labile protecting groups, compositions including the compounds, methods of making the compounds and compositions and methods of using the compounds and compositions. In one aspect, the present invention is directed to a compound of the structure XO-CH 2 -SM-B-A. The substituent X is H, an acid labile protecting group, a solid support, -P(O-R 1) NR 2 R 3 , - P(O)(OH)H, -P(O)(OR 1 )H, -P(O)(OH) 2 , -P(O)(OH)O-P(0)(OH)OP(O)(OH) 2 or salts thereof. The substituent R 1 is CNE ( i.e ., cyanoethyl), alkyl, or heteroalkyl and R 2 and R 3 are independently alkyl. The substituent SM is a sugar moiety or analogue thereof that is not a natural furanosyl, B is a base moiety or analogue thereof, and A is a moiety attached to a nitrogen on or in the base moiety of the structure -C(O)OR 4 , wherein R 4 is tertiary alkyl.
摘要翻译：本发明一般涉及包含一种或多种热不稳定保护基团的化合物，包括该化合物的组合物，制备化合物的方法和组合物以及使用该化合物和组合物的方法。一方面，本发明涉及XO-CH2-SM-B-A结构的化合物。取代基X为H，酸不稳定保护基，固体载体，-P（O-R1）NR2R3，-P（O）（OH）H，-P（O）（OR1）H，-P（O）（OH）2，-P（O）（OH）OP（O）（OH）OP（O）（OH）2或其盐。取代基R 1是CNE（即氰乙基），烷基或杂烷基，R 2和R 3独立地是烷基。取代基SM是不是天然呋喃糖基的糖部分或其类似物，B是碱基部分或其类似物，A是与结构-C（O）OR 4的碱基部分上或其上的氮连接的部分其中R4是叔烷基。

8. 发明申请

WO2014137474A1 MOLECULAR IMAGING AND RELATED METHODS 审中-公开
标题翻译：分子成像及相关方法
公开(公告)号：WO2014137474A1
公开(公告)日：2014-09-12
申请号：PCT/US2014/000027
申请日：2014-03-04
申请人： BIOSEARCH TECHNOLOGIES, INC.
发明人： BEAL, Marc , COOK, Ronald, M. , COASSIN, Peter, Jay , ORJALO, Arturo , RYU, Jekwan
IPC分类号： G01N33/48 , C12Q1/68 , G01N21/63
CPC分类号： C12Q1/6841 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , G01N21/6428 , G01N21/6458 , G02B21/0004 , G02B21/0096 , G02B21/082 , G02B21/16 , G02B21/18 , C12Q2537/143 , C12Q2543/10 , C12Q2563/107 , C12Q2565/601
摘要： The present invention generally relates to imaging single molecules, and methods related to the imaging. The method comprises the steps of: a) exposing a test sample to a probe comprising a first portion that specifically binds to a target molecule and a second portion that is detectable as the result of one or more chemical groups that interact with light, wherein the probe binds to a target molecule to provide a complex; b) exposing the complex to one or more wavelengths of light; c) detecting a result from the interaction of one or more wavelengths of light with the one or more chemical groups to provide an image of one or more single molecules. The image possesses a resolution better than 450 nm over a view field area of at least 1 x 10 5 μm 2 , and is obtained in a single detection step without variation of any detection settings.
摘要翻译：本发明一般涉及单分子成像以及与成像有关的方法。该方法包括以下步骤：a）将测试样品暴露于包含特异性结合靶分子的第一部分的探针和作为与光相互作用的一个或多个化学基团的结果可检测的第二部分，其中探针结合靶分子以提供复合物; b）将复合物暴露于一个或多个波长的光; c）检测来自一个或多个波长的光与所述一个或多个化学基团的相互作用的结果，以提供一个或多个单个分子的图像。该图像在至少1×105μm2的视场区域中具有比450nm更好的分辨率，并且在单个检测步骤中获得，而没有任何检测设置的变化。

9. 发明申请

WO2004037907A2 LUMINESCENT METAL ION COMPLEXES 审中-公开
标题翻译：发光金属离子复合物
公开(公告)号：WO2004037907A2
公开(公告)日：2004-05-06
申请号：PCT/US2003/033529
申请日：2003-10-21
申请人： BIOSEARCH TECHNOLOGIES, INC. , COOK, Ronald, M. , LYTTLE, Matt , JOHANSSON, Mary, Katherine
发明人： COOK, Ronald, M. , LYTTLE, Matt , JOHANSSON, Mary, Katherine
IPC分类号： C08K
CPC分类号： C07F15/0053 , C07B2200/11 , C09B56/00 , C09B57/10 , Y10T436/143333
摘要： The present invention provides luminescent metal ion complexes for use in a wide range of biological and chemical studies. The luminescent metal ion complexes of the invention comprise a metal ion chelating component covalently bound to a carrier molecule. Also provided are methods of making and using the luminescent metal ion complexes.
摘要翻译：本发明提供用于广泛的生物和化学研究的发光金属离子络合物。本发明的发光金属离子络合物包含与载体分子共价结合的金属离子螯合组分。还提供了制造和使用发光金属离子络合物的方法。

10. 发明公开

EP2965078A1 MOLECULAR IMAGING AND RELATED METHODS 无效转让
标题翻译：分子成像和相关方法
公开(公告)号：EP2965078A1
公开(公告)日：2016-01-13
申请号：EP14760466.4
申请日：2014-03-04
申请人： Biosearch Technologies, Inc.
发明人： BEAL, Marc , COOK, Ronald, M. , COASSIN, Peter, Jay , ORJALO, Arturo , RYU, Jekwan
IPC分类号： G01N33/48 , C12Q1/68 , G01N21/63
CPC分类号： C12Q1/6841 , C12Q1/6883 , C12Q1/6886 , C12Q2600/158 , G01N21/6428 , G01N21/6458 , G02B21/0004 , G02B21/0096 , G02B21/082 , G02B21/16 , G02B21/18 , C12Q2537/143 , C12Q2543/10 , C12Q2563/107 , C12Q2565/601
摘要： The present invention generally relates to imaging single molecules, and methods related to the imaging. The method comprises the steps of: a) exposing a test sample to a probe comprising a first portion that specifically binds to a target molecule and a second portion that is detectable as the result of one or more chemical groups that interact with light, wherein the probe binds to a target molecule to provide a complex; b) exposing the complex to one or more wavelengths of light; c) detecting a result from the interaction of one or more wavelengths of light with the one or more chemical groups to provide an image of one or more single molecules. The image possesses a resolution better than 450 nm over a view field area of at least 1 x 10
5 μm
2 , and is obtained in a single detection step without variation of any detection settings.
摘要翻译：本发明一般涉及成像单分子，以及涉及成像的方法。该方法包括以下步骤：a）将测试样品暴露于包含特异性结合靶分子的第一部分和作为与光相互作用的一个或多个化学基团的结果可检测的第二部分的探针，其中所述探针结合靶分子以提供复合物; b）将复合物暴露于一种或多种波长的光; c）检测来自一个或多个波长的光与所述一个或多个化学基团的相互作用的结果，以提供一个或多个单分子的图像。该图像在至少1×10 5μm2的视场区域上具有优于450 nm的分辨率，并且在单个检测步骤中获得，而不会改变任何检测设置。

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