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1. 发明申请

WO2005116217A1 GLUCOSE ISOMERASE MUTANTS 审中-公开
标题翻译：葡萄糖异构体突变体
公开(公告)号：WO2005116217A1
公开(公告)日：2005-12-08
申请号：PCT/CN2004/000876
申请日：2004-07-28
申请人： BIORIGHT WORLDWIDE COMPANY LIMITED , WANG, Jun , FU, Rongzhao , SHEN, Dong , JIN, Caike , LIU, Zhaoming , CHEN, Junming
发明人： WANG, Jun , FU, Rongzhao , SHEN, Dong , JIN, Caike , LIU, Zhaoming , CHEN, Junming
IPC分类号： C12N15/61
CPC分类号： C12N9/92
摘要： This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt% or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt% fructose.
摘要翻译：本发明描述了具有改进的催化活性和热稳定性的一系列重组的热酵母杆菌葡糖异构酶。重组葡萄糖异构酶可用于直接生产含有55重量％或更高浓度果糖的果糖糖浆，或用于生产含有小于55重量％果糖的果糖糖浆。

2. 发明公开

EP1956083A1 GLUCOSE ISOMERASE MUTANTS, THE USE THEREOF AND THE DNAS ENCODING THE SAME 失效转让
标题翻译：葡萄糖异构酶-MUTANTEN，DEREN VERWENDUNG UNDDDAFÜRCODIERENDEN DNAS
公开(公告)号：EP1956083A1
公开(公告)日：2008-08-13
申请号：EP06805105.1
申请日：2006-10-30
申请人： Bioright Worldwide Company Limited
发明人： WANG, Jun , JIN, Caike , FU, Rongzhao , SHEN, Dong
IPC分类号： C12N9/92 , C12N15/61 , C12N15/63 , C12P19/24
CPC分类号： C12N9/92 , C12P19/24
摘要： This invention provides a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases with improved catalytic activity and thermostability obtained by using recombinant techniques. These recombinant glucose isomerases comprise amino acid variation including phenylalanine (Phe) at position 139, alanine (Ala) at position 182, serine (Ser) at position 187, and glutamine (Gln) at position 299, and carry at least one additional mutated amino acid at position 87, position 217, position 260 or position 276, and possess a higher catalytic activity than that of the wild-type when using D-glucose as substrate. These recombinant glucose isomerases can be used for direct production of high fructose corn syrup containing 55.% [wt] or higher concentration of fructose.
摘要翻译：本发明提供了一系列通过使用重组技术获得的具有改进的催化活性和热稳定性的重组热酵母杆菌葡糖异构酶。这些重组葡萄糖异构酶包含氨基酸变异，包括139位的苯丙氨酸（Phe），182位的丙氨酸（Ala），第187位的丝氨酸（Ser）和299位的谷氨酰胺（Gln），并携带至少一种另外的突变氨基在第87位，217位，260位或276位具有较高的催化活性，当使用D-葡萄糖作为底物时，其具有比野生型高的催化活性。这些重组葡萄糖异构酶可用于直接生产含有55％（wt）或更高浓度果糖的高果糖玉米糖浆。

3. 发明授权

EP1766026B1 GLUCOSE ISOMERASE MUTANTS 有权
标题翻译：葡萄糖突变体
公开(公告)号：EP1766026B1
公开(公告)日：2011-02-02
申请号：EP04762014.1
申请日：2004-07-28
申请人： Bioright Worldwide Company Limited
发明人： WANG, Jun , FU, Rongzhao , SHEN, Dong , JIN, Caike , LIU, Zhaoming Unit 506-7, 5/F Bio-Informatics Centre , CHEN, Junming Room 607, Building No. 2
IPC分类号： C12N15/61 , C12N9/92
CPC分类号： C12N9/92
摘要： This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt% or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt% fructose.

4. 发明授权

EP1970443B1 CARRIER FOR MAKING IMMOBILIZED ENZYME OR IMMOBILIZED CELLS AND METHOD OF USING THE SAME 失效转让
标题翻译：支持固定化酶的生产或固定化细胞和方法及其使用
公开(公告)号：EP1970443B1
公开(公告)日：2013-07-17
申请号：EP06791101.6
申请日：2006-09-25
申请人： BioRight Worldwide Company Limited
发明人： JIN, Caike , WANG, Jun
IPC分类号： C12N11/06 , C12N11/08 , C12N9/92 , C12R1/19
CPC分类号： C12N11/06 , C12N11/08

5. 发明公开

EP1970443A1 CARRIER FOR MAKING IMMOBILIZED ENZYME OR IMMOBILIZED CELLS AND METHOD OF USING THE SAME 失效转让
标题翻译：支持固定化酶的生产或固定化细胞和方法及其使用
公开(公告)号：EP1970443A1
公开(公告)日：2008-09-17
申请号：EP06791101.6
申请日：2006-09-25
申请人： Bioright Worldwide Company Limited
发明人： JIN, Caike , WANG, Jun
IPC分类号： C12N11/06 , C12N11/08 , C12N9/92 , C12R1/19
CPC分类号： C12N11/06 , C12N11/08
摘要： The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes.
摘要翻译：本发明盘松动从有机发泡具有用于酶或细胞固定化的开孔和用于制备固定化酶或细胞中的方法制备的载体。本发明使用絮凝和交联技术，以固定在具有开放孔的有机泡沫材料的酶或蛋白质的细胞。得到的固定产物具有较大的比表面积，较高的比活性，可以做成各种形状。

6. 发明公开

EP1766026A1 GLUCOSE ISOMERASE MUTANTS 有权
标题翻译：葡萄糖异构酶突变体
公开(公告)号：EP1766026A1
公开(公告)日：2007-03-28
申请号：EP04762014.1
申请日：2004-07-28
申请人： Bioright Worldwide Company Limited
发明人： WANG, Jun , FU, Rongzhao , SHEN, Dong , JIN, Caike , LIU, Zhaoming Room C405, Post Graduate Hall , CHEN, Junming Room 607, Building No. 2
IPC分类号： C12N15/61 , C12N9/92
CPC分类号： C12N9/92
摘要： This invention describes a series of recombinant Thermoanaerobacterium saccharolyticum glucose isomerases having improved catalytic activity and thermostability. The recombinant glucose isomerases can be used for direct production of fructose syrup containing 55 wt% or higher concentration of fructose, or used for the production of fructose syrup containing less than 55 wt% fructose.
摘要翻译：本发明描述了一系列具有改善的催化活性和热稳定性的重组解糖热厌氧杆菌糖酵解葡萄糖异构酶。重组葡萄糖异构酶可用于直接生产含有55重量％或更高浓度果糖的果糖糖浆，或用于生产含有低于55重量％果糖的果糖糖浆。

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