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    • 1. 发明申请
    • REVERSE TRANSCRIPTASE HAVING IMPROVED THERMOSTABILITY
    • 逆转录酶具有改善的热稳定性
    • WO2012108672A3
    • 2012-12-20
    • PCT/KR2012000894
    • 2012-02-07
    • BIONEER CORPPARK HAN OHYANG SUNG JUNJOO SUNG MOHWANG BYOUNG OH
    • PARK HAN OHYANG SUNG JUNJOO SUNG MOHWANG BYOUNG OH
    • C12N9/12C12N15/54C12N15/63
    • C12N9/1276C12N1/20C12N9/12C12N15/70C12P19/34Y02P20/52
    • The present invention relates to a reverse transcriptase having improved thermostability, more precisely a mutant reverse transcriptase with improved thermostability by substitution of one or more amino acids selected from the group consisting of the 63rd glutamine (Q63), the 264th lysine (K264), the 295th lysine (K295), the 306th threonine (T306), the 346th glutamic acid (E346), the 408th proline (P408), the 438th histidine (H438), and the 454th asparagin (N454) of the amino acid sequence of M-MLV originated reverse transcriptase represented by SEQ. ID. NO: 1 with other amino acids. The mutant reverse transcriptase of the present invention demonstrates excellent thermostability, compared with the wild type reverse transcriptase. Therefore, it is advantageous to obtain the target cDNA with stable reverse transcription activity even in the presence of RNA that can form the stable secondary structure at a high temperature.
    • 本发明涉及具有改善的热稳定性的逆转录酶,更准确地说是通过取代选自第63位谷氨酰胺(Q63),第264位赖氨酸(K264),第63位赖氨酸 M-氨基酸序列的第295位赖氨酸(K295),第306位苏氨酸(T306),第346位谷氨酸(E346),第408位脯氨酸(P408),第438位组氨酸(H438)和第454位天冬酰胺(N454) MLV来源于SEQ.ID.No.1所示的逆转录酶。 ID。 NO:1与其他氨基酸。 与野生型逆转录酶相比,本发明的突变逆转录酶显示出优异的热稳定性。 因此,即使在能够在高温下形成稳定的二级结构的RNA的存在下获得具有稳定逆转录活性的靶cDNA也是有利的。