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    • 5. 发明申请
    • SALT RESISTANT TRANSGENIC PLANTS
    • 耐盐转基因植物
    • WO2006025059A2
    • 2006-03-09
    • PCT/IL2005/000932
    • 2005-09-01
    • YEDA RESEARCH AND DEVELOPMENT CO. LTD. at the Weizmann Institute of ScienceRAHAN MERISTEM (1998) LTD.HAZERA GENETICS LTD.PICK, UriLIVNEH, OrnaKHAYAT, EliWALTHER, RinaGABAI, Avi
    • PICK, UriLIVNEH, OrnaKHAYAT, EliWALTHER, RinaGABAI, Avi
    • C12N15/8273C12N9/14
    • The present invention provides transgenic plants transformed with exogenous nucleic acid encoding a Dunaliella plasma membrane (PM)-ATPase. The transgenic plant has increased tolerance to salt as compared to a corresponding non-transgenic plant. The present invention also provides nucleic acids encoding a chimeric PM-ATPase, which comprise a first portion encoding a plant PM-ATPase or a fragment thereof, and a second portion encoding a Dunaliella PM-ATPase or a fragment thereof. The present invention also discloses a method of producing a transgenic plant having an increased tolerance to salt as compared to a corresponding non-transgenic plant, a method of modifying a plant capacity to survive salt shock, and a method of modifying plant recovery after exposure to salt stress, by introducing into one or more cells of a plant an exogenous nucleic acid encoding a Dunaliella PM-ATPase. Also provided by the present invention are plant cells comprising an exogenous nucleic acid encoding a Dunaliella PM-ATPase, and plant seeds and progeny obtained from the transgenic plants.
    • 本发明提供了用编码杜氏藻质粒(PM)-ATPase的外源核酸转化的转基因植物。 与相应的非转基因植物相比,转基因植物对盐的耐受性增加。 本发明还提供编码嵌合PM-ATP酶的核酸,其包含编码植物PM-ATP酶或其片段的第一部分,以及编码杜氏藻属PM-ATP酶或其片段的第二部分。 本发明还公开了一种生产与相应的非转基因植物相比具有增加的耐盐性的转基因植物的方法,改变植物体内耐盐胁迫的能力的方法,以及在暴露于 盐胁迫,通过向植物的一个或多个细胞中引入编码杜氏藻属PM-ATP酶的外源核酸。 本发明还提供了包含编码杜氏藻属PM-ATP酶的外源核酸和从转基因植物获得的植物种子和后代的植物细胞。