专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1993017125A1 OLIGONUCLEOTIDE MODULATION OF CELL GROWTH 审中-公开
标题翻译：细胞生长的寡核苷酸调节
公开(公告)号：WO1993017125A1
公开(公告)日：1993-09-02
申请号：PCT/US1993000754
申请日：1993-01-27
申请人： BAYLOR COLLEGE OF MEDICINE , ISIS PHARMACEUTICALS, INC. , BUSCH, Harris , BENNETT, Clarence, Frank , PERLAKY, Laszlo , SAIJO, Yasuo , BUSCH, Rose, K.
发明人： BAYLOR COLLEGE OF MEDICINE , ISIS PHARMACEUTICALS, INC.
IPC分类号： C12Q01/68
CPC分类号： C12N15/113 , A61K38/00 , C12N2310/315 , C12N2310/321 , C12N2310/3521 , C12Q1/6886 , C12Q2600/136
摘要： Oligonucleotides capable of inhibiting the production of proliferation associated proteins are provided. Oligonucleotides designed to be hybridizable with nucleic acids encoding nucleolar proteins are believed to be therapeutically useful. Certain of such oligonucleotides, hybridizable to portions of the gene coding for p120, especially the 3' untranslated region, were made and found to inhibit the synthesis of p120. The oligonucleotides of the invention are useful for the treatment of diseases characterized by hyperproliferation of cells such as malignancies, inflammatory and cardiovascular diseases. Treatment of human breast cell carcinoma, human epitheloid cervix carcinoma, human amelanotic malanoma and human renal cell carcinoma and other tumors are indicated.
摘要翻译：提供能够抑制增殖相关蛋白产生的寡核苷酸。设计为可与编码核仁蛋白的核酸杂交的寡核苷酸被认为是治疗上有用的。制备了与编码p120的基因的部分杂交的某些这样的寡核苷酸，特别是3'非翻译区，发现抑制p120的合成。本发明的寡核苷酸可用于治疗特征为细胞过度增殖的疾病，例如恶性肿瘤，炎性和心血管疾病。表明人乳腺癌细胞癌，人类上皮细胞癌宫颈癌，人类无黑色素瘤恶性肿瘤和人肾细胞癌等肿瘤的治疗。

2. 发明申请

WO1995010938A1 OLIGONUCLEOTIDE MODULATION OF MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 审中-公开
标题翻译：多重耐药相关蛋白的寡核苷酸调节
公开(公告)号：WO1995010938A1
公开(公告)日：1995-04-27
申请号：PCT/US1994010827
申请日：1994-09-23
申请人： ISIS PHARMACEUTICALS, INC. , BARACCHINI, Edgardo, Jr. , BENNETT, Clarence, Frank
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： A01N43/04
CPC分类号： C12N15/1138 , A61K38/00 , C07H21/00 , C12N2310/315 , C12N2310/321 , C12N2310/322 , C12N2310/3517 , C12N2310/3527 , C12N2310/3521
摘要： Compositions and methods are provided for the treatment and diagnosis of diseases or conditions amenable to treatment through modulation of the synthesis or metabolism of multidrug resistance-associated protein (MRP). In accordance with preferred embodiments, oligonucleotides are provided which are specifically hybridizable with nucleic acids encoding multidrug resistance-associated protein (MRP). Methods of treating animals suffering from diseases or conditions amenable to therapeutic intervention by modulating multidrug resistance with an oligonucleotide specifically hybridizable with RNA or DNA corresponding to multidrug resistance-associated protein (MRP) are disclosed. Methods of preventing the development of multidrug resistance and of improving the efficacy of chemotherapy are also disclosed.
摘要翻译：提供组合物和方法用于治疗和诊断适于通过调节多药耐药相关蛋白（MRP）的合成或代谢来治疗的疾病或病症。根据优选实施方案，提供了与编码多药耐药相关蛋白（MRP）的核酸特异性杂交的寡核苷酸。公开了通过用与多药耐药相关蛋白（MRP）相应的RNA或DNA特异性杂交的寡核苷酸调节多药耐药性来治疗患有治疗性干预的疾病或病症的动物的方法。还公开了预防多药耐药性发展和提高化学疗效的方法。

3. 发明申请

WO1993007883A1 DERIVATIZED OLIGONUCLEOTIDES HAVING IMPROVED UPTAKE AND OTHER PROPERTIES 审中-公开
标题翻译：具有改进的摄取和其他性质的衍生的寡核苷酸
公开(公告)号：WO1993007883A1
公开(公告)日：1993-04-29
申请号：PCT/US1992009196
申请日：1992-10-23
申请人： ISIS PHARMACEUTICALS, INC. , MANOHARAN, Muthiah , COOK, Philip, Dan , BENNETT, Clarence, Frank
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： A61K31/70
CPC分类号： C07H21/00 , C07H23/00 , C07J43/003 , C07J51/00
摘要： Linked nucleosides having at least one functionalized nucleoside that bears a substituent such as a steroid molecule, a reporter molecule, a non-aromatic lipophilic molecule, a reporter enzyme, a peptide, a protein, a water soluble vitamin, a lipid soluble vitamin, an RNA cleaving complex, a metal chelator, a porphyrin, an alkylator, a pyrene, a hybrid photonuclease/intercalator, or an aryl azide photo-crosslinking agent exhibit increased cellular uptake and other properties. The substituent can be attached at the 2'-position of the functionalized nucleoside via a linking group. If at least a portion of the remaining linked nucleosides are 2'-deoxy-2'-fluoro, 2'-O-methoxy, 2'-O-ethoxy, 2'-O-propoxy, 2'-O-aminoalkoxy or 2'-O-allyloxy nucleosides, the substituent can be attached via a linking group at any of the 3' or the 5' positions of the nucleoside or on the heterocyclic base of the nucleoside or on the inter-nucleotide linkage linking the nucleoside to an adjacent nucleoside.
摘要翻译：具有至少一个具有取代基的官能化核苷的连接核苷，例如类固醇分子，报道分子，非芳族亲脂性分子，报道酶，肽，蛋白质，水溶性维生素，脂溶性维生素， RNA切割复合物，金属螯合剂，卟啉，烷基化剂，芘，混合光子核酸酶/嵌入剂或芳基叠氮化物光交联剂表现出增加的细胞摄取和其它性质。取代基可以通过连接基团连接在官能化核苷的2'-位上。如果至少一部分剩余连接的核苷是2'-脱氧-2'-氟，则2'-O-甲氧基，2'-O-乙氧基，2'-O-丙氧基，2'-O-氨基烷氧基或2 '-O-烯丙氧基核苷，取代基可以通过连接基团在核苷的3'或5'位置或核苷的杂环碱基上或连接核苷与核苷酸之间的核苷酸间连接相邻核苷。

4. 发明申请

WO1991016901A1 OLIGONUCLEOTIDE MODULATION OF ARACHIDONIC ACID METABOLISM 审中-公开
标题翻译：阿昔洛韦酸代谢的寡糖调节
公开(公告)号：WO1991016901A1
公开(公告)日：1991-11-14
申请号：PCT/US1991002628
申请日：1991-04-17
申请人： ISIS PHARMACEUTICALS, INC. , BENNETT, Clarence, Frank , ECKER, David, J. , CROOKE, Stanely, T. , MIRABELLI, Christopher, K.
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： A61K31/70
CPC分类号： C12N15/1137 , C12N9/0006 , C12N2310/15 , C12N2310/315 , C12Q1/68 , C12Q1/6839 , C12Q1/6883 , C12Q2600/158 , C12Y113/11012 , C12Y301/01004 , C12Y301/04003 , C12Y303/02006 , G01N33/88
摘要： Compositions and methods are provided for the treatment and diagnosis of diseases amenable to modulation of the synthesis or metabolism of arachidonic acid and related compounds. In accordance with preferred embodiments, oligonucleotides and oligonucleotide analogs are provided which are specifically hybridizable with nucleic acids encoding 5-lipoxygenase, 5-lipoxygenase activating proteins, LTA4 hydrolase, phospholipase A2, phospholipase C, and coenzyme A-independent transacylase. The oligonucleotide comprises nucleotide units sufficient in identity and number to effect said specific hybridization. In other preferred embodiments, the oligonucleotides are specifically hybridizable with a transcription initiation site, a translation initiation site, and intron/exon junction. Methods of treating animals suffering from disease amenable to therapeutic intervention by modulating arachidonic acid synthesis or metabolism with an oligonucleotide or oligonucleotide analog specifically hybridizable with RNA or DNA corresponding to one of the foregoing proteins are disclosed. Methods for treatment of diseases responding to modulation of arachidonic acid synthesis or metabolism are disclosed.
摘要翻译：提供组合物和方法用于治疗和诊断适于调节花生四烯酸和相关化合物的合成或代谢的疾病。根据优选实施方案，提供寡核苷酸和寡核苷酸类似物，其与编码5-脂氧合酶，5-脂氧合酶活化蛋白，LTA4水解酶，磷脂酶A2，磷脂酶C和辅酶A非转基因酶的核酸特异性杂交。所述寡核苷酸包含足以实现所述特异性杂交的同一性和数目的核苷酸单元。在其它优选的实施方案中，寡核苷酸与转录起始位点，翻译起始位点和内含子/外显子连接区特异性杂交。公开了通过用与上述蛋白质之一相对应的RNA或DNA特异性杂交的寡核苷酸或寡核苷酸类似物调节花生四烯酸合成或代谢来治疗适于治疗性干预的疾病的方法。公开了治疗花生四烯酸合成或代谢调节的疾病的方法。

5. 发明申请

WO1992003139A1 OLIGONUCLEOTIDE MODULATION OF CELL ADHESION 审中-公开
标题翻译：细胞粘附的寡糖调节
公开(公告)号：WO1992003139A1
公开(公告)日：1992-03-05
申请号：PCT/US1991005209
申请日：1991-07-23
申请人： ISIS PHARMACEUTICALS, INC. , BENNETT, Clarence, Frank , MIRABELLI, Christopher, K.
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： A61K31/70
CPC分类号： C12N15/1138 , A61K38/00 , C07K14/705 , C12N2310/315 , C12N2310/322 , C12N2310/3521 , C12N2310/3531 , C12N2310/3533
摘要： Compositions and methods are provided for the treatment and diagnosis of diseases amenable to treatment through modulation of the synthesis or metabolism of intercellular adhesion molecules. In accordance with preferred embodiments, oligonucleotides and oligonucleotide analogs are provided which are specifically hybridizable with nucleic acids encoding intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial leukocyte adhesion molecule-1. The oligonucleotide comprises nucleotide units sufficient in identity and number to effect said specific hybridization. In other preferred embodiments, the oligonucleotides are specifically hybridizable with a transcription initiation site, a translation initiation site, 5'-untranslated sequences, 3'-untranslated sequences, and intervening sequences. Methods of treating animals suffering from disease amenable to therapeutic intervention by modulating cell adhesion proteins with an oligonucleotide or oligonucleotide analog specifically hybridizable with RNA or DNA corresponding to one of the foregoing proteins are disclosed. Methods for treatment of diseases responding to modulation cell adhesion molecules are disclosed.
摘要翻译：提供组合物和方法，用于治疗和诊断适于通过调节细胞间粘附分子的合成或代谢来治疗的疾病。根据优选实施方案，提供寡核苷酸和寡核苷酸类似物，其与编码细胞间粘附分子-1，血管细胞粘附分子-1和内皮细胞粘附分子-1的核酸特异性杂交。所述寡核苷酸包含足以实现所述特异性杂交的同一性和数目的核苷酸单元。在其它优选实施方案中，寡核苷酸与转录起始位点，翻译起始位点，5'-非翻译序列，3'非翻译序列和插入序列特异性杂交。公开了通过用与上述蛋白质之一相应的RNA或DNA特异性杂交的寡核苷酸或寡核苷酸调节细胞粘附蛋白来治疗患有疾病的动物的治疗方法。公开了治疗响应于调节细胞粘附分子的疾病的方法。

6. 发明申请

WO1998029429A1 METHOD FOR THE SYNTHESIS OF NUCLEOTIDE OR OLIGONUCLEOTIDE PHOSPHORAMIDITES 审中-公开
标题翻译：合成核苷酸或寡核苷酸磷酸酰胺的方法
公开(公告)号：WO1998029429A1
公开(公告)日：1998-07-09
申请号：PCT/US1997023269
申请日：1997-12-16
申请人： ISIS PHARMACEUTICALS, INC. , GRIFFEY, Richard, H. , COLE, Douglas, L. , RAVIKUMAR, Vasulinga
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： C07H21/02
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00
摘要： This invention presents novel methods for recovery of phosphoramidites from the waste products of oligonucleotide synthesis. The methods include reacting a tribromophenoxydichlorophosphorane with an H-phosphonate in the presence of an amine.
摘要翻译：本发明提出了从寡核苷酸合成废物中回收亚磷酰胺的新方法。所述方法包括在胺的存在下使三溴苯氧基二氯磷烷与H-膦酸酯反应。

7. 发明申请

WO1998027425A1 LARGE-SCALE PURIFICATION OF FULL LENGTH OLIGONUCLEOTIDES BY SOLID-LIQUID AFFINITY EXTRACTION 审中-公开
标题翻译：通过固体物质萃取法对全长度寡核苷酸进行大规模的纯化
公开(公告)号：WO1998027425A1
公开(公告)日：1998-06-25
申请号：PCT/US1997023284
申请日：1997-12-18
申请人： ISIS PHARMACEUTICALS, INC. , CHEN, Danhua , SRIVATSA, Githa, Susan , COLE, Douglas, L.
发明人： ISIS PHARMACEUTICALS, INC.
IPC分类号： G01N33/48
CPC分类号： A61K48/0091 , C12Q1/6837 , C12Q2525/107
摘要： Methods and matrices are provided for purifying a desired target oligonucleotide using an immobilized affinity unit that selectively binds the target oligonucleotide. The immobilized affinity unit preferably comprises a nucleobase sequence which comprises the reverse complement of the target oligonucleotide. Preferred methods of the invention result in the rapid, cost-effective and efficacious separation of most undesired contaminants, particularly undesired deletion [e.g., (n-1), (n-2), etc.] derivatives of the desired full length (n) oligonucleotide that result from incomplete oligomerization or through degradative processes, including those undesired derivatives having internal or 3' deletions of one or more nucleotides.
摘要翻译：提供了使用选择性结合靶寡核苷酸的固定化亲和单元纯化所需靶寡核苷酸的方法和基质。固定化亲和单元优选包含核碱基序列，其包含靶寡核苷酸的反向互补序列。本发明的优选方法导致大多数不需要的污染物的快速，成本有效和有效的分离，特别是期望全长（n（n-1），n-1），（n-2））寡核苷酸，其由不完全寡聚化或通过降解过程产生，包括具有一个或多个核苷酸的内部或3'缺失的那些不期望的衍生物。

8. 发明申请

WO1998020349A1 COMPLEX CHEMICAL LIBRARIES 审中-公开
标题翻译：复合化学图书馆
公开(公告)号：WO1998020349A1
公开(公告)日：1998-05-14
申请号：PCT/US1997020155
申请日：1997-11-04
申请人： ISIS PHARMACEUTICALS, INC.
发明人： ISIS PHARMACEUTICALS, INC. , COOK, Phillip, Dan , AN, Haoyun
IPC分类号： G01N33/53
CPC分类号： C40B50/14 , C40B40/04
摘要： Compositions comprising novel chemical libraries are prepared. The compositions of the present invention are useful as antibacterial and other pharmaceutical agents and as intermediates for preparation of other pharmaceutical agents. In addition, compounds of the present invention are useful as research reagents.
摘要翻译：制备包含新化学文库的组合物。本发明的组合物可用作抗细菌剂和其它药剂以及作为制备其它药剂的中间体。此外，本发明的化合物可用作研究试剂。

9. 发明申请

WO1998011123A1 CARBAMATE-DERIVATIZED NUCLEOSIDES AND OLIGONUCLEOSIDES 审中-公开
标题翻译：碳酸盐衍生的核苷酸和寡核苷酸
公开(公告)号：WO1998011123A1
公开(公告)日：1998-03-19
申请号：PCT/US1997015970
申请日：1997-09-10
申请人： ISIS PHARMACEUTICALS, INC.
发明人： ISIS PHARMACEUTICALS, INC. , COOK, Phillip, Dan , MANOHARAN, Muthiah
IPC分类号： C07H21/02
CPC分类号： C07H21/00 , C07H19/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H19/20 , C07J1/00
摘要： Nucleosides and oligonucleosides functionalized to include carbamate functionality, and derivatives thereof. In certain embodiments, the compounds of the invention further include steroids, reporter molecules, reporter enzymes, lipophilic molecules, peptides or proteins attached to the nucleosides through the carbamate group.
摘要翻译：功能化为包括氨基甲酸酯官能团的核苷和寡核苷酸及其衍生物。在某些实施方案中，本发明的化合物还包括通过氨基甲酸酯基团连接到核苷上的类固醇，报告分子，报道酶，亲脂性分子，肽或蛋白质。

10. 发明申请

WO1997046570A1 OLIGORIBONUCLEOTIDES AND RIBONUCLEASES FOR CLEAVING RNA 审中-公开
标题翻译：用于清除RNA的寡核苷酸和核糖核酸
公开(公告)号：WO1997046570A1
公开(公告)日：1997-12-11
申请号：PCT/US1997009963
申请日：1997-06-06
申请人： ISIS PHARMACEUTICALS, INC.
发明人： ISIS PHARMACEUTICALS, INC. , CROOKE, Stanley, T.
IPC分类号： C07H21/00
CPC分类号： C12N15/113 , A61K38/00 , C07H21/00 , C12N9/22 , C12N15/1135 , C12N2310/311 , C12N2310/312 , C12N2310/314 , C12N2310/315 , C12N2310/316 , C12N2310/318 , C12N2310/3181 , C12N2310/321 , C12N2310/322 , C12N2310/3341 , C12N2310/335 , C12N2310/341 , C12N2310/346 , Y02A50/395 , Y02P20/582 , C12N2310/3527 , C12N2310/3525 , C12N2310/3521
摘要： Oligomeric compounds including oligoribonucleotides and oligoribonucleosides are provided that have subsequences of 2'-pentoribofuranosyl nucleosides that activate dsRNase. The oligoribonucleotides and oligoribonucleosides can include substituent groups for increasing binding affinity to complementary nucleic acid strand as well as substituent groups for increasing nuclease resistance. The oligomeric compounds are useful for diagnostics and other research purposes, for modulating the expression of a protein in organisms, and for the diagnosis, detection and treatment of other conditions susceptible to oligonucleotide therapeutics. Also included in the invention are mammalian ribonucleases, i.e., enzymes that degrade RNA, and substrates for such ribonucleases. Such ribonuclease is referred to herein as a dsRNase, wherein "ds" indicates the RNase's specificity for certain double-stranded RNA substrates. The artificial substrates for the dsRNases described herein are useful in preparing affinity matrices for purifying mammalian ribonuclease as well as non-degradative RNA-binding proteins.
摘要翻译：提供了包含寡核糖核苷酸和寡核糖核苷的寡聚化合物，其具有激活dsRNase的2'-戊二呋呋糖基核苷的亚序列。寡核糖核苷酸和寡核糖核苷可以包括用于增加对互补核酸链的结合亲和力的取代基以及用于增加核酸酶抗性的取代基。寡聚化合物可用于诊断和其他研究目的，用于调节生物体中的蛋白质的表达，以及用于诊断，检测和治疗对寡核苷酸治疗剂敏感的其它病症。本发明还包括哺乳动物核糖核酸酶，即降解RNA的酶和这种核糖核酸酶的底物。这种核糖核酸酶在本文中称为dsRNase，其中“ds”表示RNase对某些双链RNA底物的特异性。本文所述的dsR酶的人造底物可用于制备用于纯化哺乳动物核糖核酸酶以及非降解性RNA结合蛋白的亲和基质。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式