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1. 发明申请

WO2005095632A2 METHODS FOR IDENTIFYING PROTEINS WITH STARCH PHOSPHORYLATING ENZYMATIC ACTIVITY 审中-公开
标题翻译：用淀粉磷酸化酶活性鉴定蛋白质的方法
公开(公告)号：WO2005095632A2
公开(公告)日：2005-10-13
申请号：PCT/EP2005/002454
申请日：2005-03-04
申请人： BAYER CROPSCIENCE GMBH , FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12Q1/00
CPC分类号： C12N9/12 , C12N15/8245 , C12Q1/485 , G01N33/573 , G01N2400/14 , G01N2500/04
摘要： The present invention relates to a method for identifying proteins involved in the phosphorylation of starch and nucleic acids which code for such proteins. The present invention further relates to plant cells and plants which exhibit an altered activity of a protein which can be identified using the method according to the invention. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of this starch and to the manufacture of starch derivatives of this modified starch.
摘要翻译：本发明涉及一种鉴定涉及淀粉磷酸化的蛋白质的方法以及编码这种蛋白质的核酸。本发明还涉及可以使用本发明的方法鉴定的蛋白质活性变化的植物细胞和植物。这种植物细胞和植物合成改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉以及制备该淀粉的方法和该淀粉衍生物的制造。

2. 发明申请

WO2005095618A2 PLANTS WITH REDUCED ACTIVITY OF A STARCH PHOSPHORYLATING ENZYME 审中-公开
标题翻译：具有降低淀粉酶活性的植物
公开(公告)号：WO2005095618A2
公开(公告)日：2005-10-13
申请号：PCT/EP2005/002450
申请日：2005-03-04
申请人： BAYER CROPSCIENCE GmbH , FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82
CPC分类号： C12N9/00 , C08B31/066 , C12N9/1294 , C12N15/8245
摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OK1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules, which are suitable for manufacturing plants according to the invention.
摘要翻译：本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性降低，与相应的野生型植物细胞或未经遗传修饰的野生型植物相比较。此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。这种植物细胞和植物合成改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制备，以及含有本发明淀粉的面粉。此外，本发明涉及适合于制造根据本发明的植物的核酸分子。

3. 发明申请

WO2005095619A1 PLANTS WITH INCREASED ACTIVITY OF MULTIPLE STARCH PHOSPHORYLATING ENZYMES 审中-公开
标题翻译：具有增加多种淀粉酶活性的植物
公开(公告)号：WO2005095619A1
公开(公告)日：2005-10-13
申请号：PCT/EP2005/002457
申请日：2005-03-04
申请人： BAYER CROPSCIENCE GMBH , FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82
CPC分类号： C12N15/8245 , C08B31/066 , C12N9/00
摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the increase of the activity of a starch phosphorylating OK1 protein and a starch phosphorylating R1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules and vectors containing sequences which code for an OK1 protein and an RI protein, as well as host cells which contain these nucleic acid molecules.
摘要翻译：本发明涉及遗传修饰的植物细胞和植物，其中与相应的野生型植物细胞或野生型植物相比，遗传修饰导致淀粉磷酸化OK1蛋白和淀粉磷酸化R1蛋白的活性增加没有转基因。此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。这种植物细胞和植物合成改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这种淀粉的制造方法，以及该改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉。此外，本发明涉及含有编码OK1蛋白和RI蛋白的序列的核酸分子和载体以及含有这些核酸分子的宿主细胞。

4. 发明申请

WO2005095617A2 PLANTS WITH INCREASED ACTIVITY OF A STARCH PHOSPHORYLATING ENZYME 审中-公开
标题翻译：具有增加淀粉酶活性的植物
公开(公告)号：WO2005095617A2
公开(公告)日：2005-10-13
申请号：PCT/EP2005/002449
申请日：2005-03-04
申请人： BAYER CROPSCIENCE GMBH , FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82
CPC分类号： B02C9/00 , C12N9/12 , C12N9/1205 , C12N15/8245
摘要： The present invention relates to plant cells and palnts, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starches synthesised from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.
摘要翻译：本发明涉及遗传修饰的植物细胞和植物，由此遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加转基因此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。这些类型的植物细胞和植物合成了改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法以及这些改性淀粉的淀粉衍生物的制备，以及含有本发明淀粉的面粉。此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有该核酸分子的植物的核酸。此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

5. 发明公开

EP1725667A1 PLANTS WITH INCREASED ACTIVITY OF MULTIPLE STARCH PHOSPHORYLATING ENZYMES 有权
标题翻译： PFLANZEN MITERHÖHTERAKTIVITÄTMEHRERERSTÄRKEPHOSPHORYLIERENDER ENZYME
公开(公告)号：EP1725667A1
公开(公告)日：2006-11-29
申请号：EP05715852.9
申请日：2005-03-04
申请人： Bayer CropScience GmbH
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82 , A01H5/00 , A01H5/04
CPC分类号： C12N15/8245 , C08B31/066 , C12N9/00
摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the increase of the activity of a starch phosphorylating OK1 protein and a starch phosphorylating R1 protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules and vectors containing sequences which code for an OK1 protein and an RI protein, as well as host cells which contain these nucleic acid molecules.
摘要翻译：本发明涉及遗传修饰的植物细胞和植物，其中与对应的野生型植物细胞或野生型植物相比，遗传修饰导致淀粉磷酸化OK1蛋白和淀粉磷酸化R1蛋白的活性增加没有转基因。此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。这种植物细胞和植物合成改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，该淀粉的制造方法和该改性淀粉的淀粉衍生物的制备，以及含有根据本发明的淀粉的面粉。此外，本发明涉及含有编码OK1蛋白和R1蛋白的序列的核酸分子和载体以及含有这些核酸分子的宿主细胞。

6. 发明公开

EP1737974A2 METHODS FOR IDENTIFYING PROTEINS WITH STARCH PHOSPHORYLATING ENZYMATIC ACTIVITY 有权
标题翻译：方法蛋白表达与STÄRKEPHOSPHORYLIERENDER酶活性鉴定
公开(公告)号：EP1737974A2
公开(公告)日：2007-01-03
申请号：EP05735946.5
申请日：2005-03-04
申请人： Bayer CropScience GmbH
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12Q1/48 , C07K14/415 , C12N5/04 , C12N15/82 , A01H5/00
CPC分类号： C12N9/12 , C12N15/8245 , C12Q1/485 , G01N33/573 , G01N2400/14 , G01N2500/04
摘要： The present invention relates to a method for identifying proteins involved in the phosphorylation of starch and nucleic acids which code for such proteins. The present invention further relates to plant cells and plants which exhibit an altered activity of a protein which can be identified using the method according to the invention. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of this starch and to the manufacture of starch derivatives of this modified starch.

7. 发明公开

EP1725666A2 PLANTS WITH REDUCED ACTIVITY OF THE STARCH PHOSPHORYLATING ENZYME PHOSPHOGLUCAN, WATER DIKINASE 有权
标题翻译：酶法STÄRKEPHOSPHORYLIERENDENPHOSPHOGLUCAN水二激酶活性降低植物
公开(公告)号：EP1725666A2
公开(公告)日：2006-11-29
申请号：EP05715846.1
申请日：2005-03-04
申请人： Bayer CropScience GmbH
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82 , C12N9/12 , C07K16/40 , C12N1/21 , C12N5/10 , C12N15/01 , C12N15/11 , C12N15/54 , A01H5/00
CPC分类号： C12N9/00 , C08B31/066 , C12N9/1294 , C12N15/8245
摘要： The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the reduction of the activity of a starch phosphorylating OKl protein in comparison with corresponding wild type plant cells or wild type plants that have not been genetically modified. The OKl protein acts as a phosphoglucan, water dikinase (PWD) which predominately phosphorylates the C3 position. Genetically modified plants in which the expression of PWD is reduced show a starch excess phenotype. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention, methods for the manufacture of this starch, and the manufacture of starch derivatives of this modified starch, as well as flours containing starches according to the invention. Furthermore, the present invention relates to nucleic acid molecules, which ar suitable for manufacturing plants according to the invention.

8. 发明公开

EP1725665A2 PLANTS WITH INCREASED ACTIVITY OF A STARCH PHOSPHORYLATING ENZYME 有权
标题翻译： PFLANZEN MITERHÖHTERAKTIVITÄTEINESSTÄRKEPHOSPHORYLIERENDENENZYMS
公开(公告)号：EP1725665A2
公开(公告)日：2006-11-29
申请号：EP05715845.3
申请日：2005-03-04
申请人： Bayer CropScience GmbH
发明人： FROHBERG, Claus , KOETTING, Oliver , RITTE, Gerhard , STEUP, Martin
IPC分类号： C12N15/82 , C12N15/29 , A01H5/00
CPC分类号： B02C9/00 , C12N9/12 , C12N9/1205 , C12N15/8245
摘要： The present invention relates to plant cells and palnts, which are genetically modified, whereby the genetic modification leads to an increase in the activity of a starch-phosphorylating OK1 protein in comparison to the corresponding wild type plant cells or wild type plants that have not been genetically modified. In addition, the present invention concerns means and methods for the manufacture of such plant cells and plants. These types of plant cells and plants synthesise a modified starch. Therefore, the present invention also concerns the starches synthesised from the plant cells and plants according to the invention, methods for manufacturing these starches, and the manufacture of starch derivatives of these modified starches, as well as flours containing starches according to the invention. Furthermore, the present invention also relates to nucleic acids, coding starch-phosphorylating OK1 proteins, vectors, host cells, plant cells, and plants containing such nucleic acid molecules. In addition, the present invention relates to OK1 proteins that have starch-phosphorylating activity.
摘要翻译：本发明涉及遗传修饰的植物细胞和植物，其中遗传修饰导致淀粉磷酸化OK1蛋白的活性与尚未被使用的相应野生型植物细胞或野生型植物相比增加转基因此外，本发明涉及用于制造这种植物细胞和植物的方法和方法。这些类型的植物细胞和植物合成了改性淀粉。因此，本发明还涉及由根据本发明的植物细胞和植物合成的淀粉，这些淀粉的制造方法以及这些改性淀粉的淀粉衍生物的制造，以及含有本发明淀粉的面粉。此外，本发明还涉及编码淀粉磷酸化OK1蛋白，载体，宿主细胞，植物细胞和含有这种核酸分子的植物的核酸。此外，本发明涉及具有淀粉磷酸化活性的OK1蛋白质。

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