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    • 8. 发明申请
    • MODIFIED PROMOTER
    • 改装促销
    • WO2011049227A1
    • 2011-04-28
    • PCT/JP2010/068783
    • 2010-10-18
    • KAO CORPORATIONKAWAHARA, AkihitoKODAMA, HiroshiARA, Katsutoshi
    • KAWAHARA, AkihitoKODAMA, HiroshiARA, Katsutoshi
    • C12N15/113
    • C12N15/75C12N1/20C12N15/11C12N15/66C12P21/02
    • The present invention provides a modified promoter, an expression vector and a transformant each containing the promoter, and a method for producing a gene product of interest using the transformant. The invention provides a modified promoter, including a nucleotide sequence of a promoter derived from bacterium belonging to the genus Bacillus in which at least one nucleotide sequence selected from the following has been modified: a nucleotide sequence represented by SEQ ID NO: 1; a nucleotide sequence equivalent to the nucleotide sequence represented by SEQ ID NO: 1, except that one or a plurality of bases therein are substituted, deleted, added or inserted; and a nucleotide sequence having a sequence identity of 70% or more with respect to the nucleotide sequence represented by SEQ ID NO: 1.
    • 本发明提供一种修饰的启动子,表达载体和各自含有启动子的转化体,以及使用该转化体产生感兴趣的基因产物的方法。 本发明提供一种修饰的启动子,其包含衍生自属于芽孢杆菌属的细菌的启动子的核苷酸序列,其中至少一个选自以下的核苷酸序列已被修饰:由SEQ ID NO:1表示的核苷酸序列; 与SEQ ID NO:1所示的核苷酸序列相当的核苷酸序列,除了其中一个或多个碱基被取代,缺失,添加或插入; 和相对于由SEQ ID NO:1表示的核苷酸序列具有70%以上的序列同一性的核苷酸序列。