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1. 发明申请

WO2008135229A3 FORMULATION OF SUGAR SOLUTIONS FOR CONTINUOUS ULTRACENTRIFUGATION FOR VIRUS PURIFICATION 审中-公开
公开(公告)号：WO2008135229A3
公开(公告)日：2008-11-13
申请号：PCT/EP2008/003531
申请日：2008-04-30
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A. , REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang , MITTERER, Artur , SCHAFHAUSER, Horst
IPC分类号： C12N7/02 , C12Q1/70
摘要： The present invention provides a method for purification of a virus or virus antigen comprising providing a virus preparation and centrifugation of said virus preparation in a gradient of a sugar established by the addition of two or more buffered sugar layers of different concentration. The method leads to higher yields and reduces unwanted aggregation of the virus or virus antigen by increasing the volume of the peak pool.

2. 发明申请

WO2013192009A1 VIRUS FILTRATION OF CELL CULTURE MEDIA 审中-公开
标题翻译：细胞培养液的病毒过滤
公开(公告)号：WO2013192009A1
公开(公告)日：2013-12-27
申请号：PCT/US2013/045663
申请日：2013-06-13
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A. , MUNDT, Wolfgang , MITTERER, Artur , REITER, Manfred , HASSLACHER, Meinhard , GRILLBERGER, Leopold , KREIL, Thomas
发明人： MUNDT, Wolfgang , MITTERER, Artur , REITER, Manfred , HASSLACHER, Meinhard , GRILLBERGER, Leopold , KREIL, Thomas
IPC分类号： C12M1/00 , A61L2/00 , C12M1/12 , C12N5/00
CPC分类号： C12N5/0602 , A61L2/0017 , C12M29/26 , C12M37/02
摘要： The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m2. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.
摘要翻译：本发明涉及从制剂，细胞培养基或至少细胞培养基组分中除去病毒污染物的方法。该方法包括使所述制剂通过具有最大约75nm的有效孔径的病毒过滤器过滤至少约24小时。此外，本发明涉及病毒过滤器在至少约24小时的过滤中的用途，其中所述病毒过滤器具有最大约75nm的有效孔径，用于从作为细胞培养基的制剂中除去病毒污染物或至少一种细胞培养基的组分。在一些实施方案中，根据本发明的过滤以至少约2000L / m 2的体积运行。此外，本发明涉及根据本发明的方法获得的用于细胞培养的制剂，细胞培养基或至少一种细胞培养基的成分的用途; 药物，诊断和/或化妆品制剂以及食品制剂。

3. 发明申请

WO2007077217A2 OLIGOPEPTIDE-FREE CELL CULTURE MEDIA 审中-公开
标题翻译：无血清细胞培养基
公开(公告)号：WO2007077217A2
公开(公告)日：2007-07-12
申请号：PCT/EP2007/000027
申请日：2007-01-03
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A. , GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang , MITTERER, Artur
发明人： GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang , MITTERER, Artur
IPC分类号： C12N5/00
CPC分类号： C07K14/755 , C12N5/0018 , C12N5/005 , C12N7/00 , C12N2500/05 , C12N2500/10 , C12N2500/20 , C12N2500/24 , C12N2500/32 , C12N2500/38 , C12N2500/46 , C12N2500/50 , C12N2710/24151
摘要： The present invention relates to oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine and to methods for cultivating cells in said oligopeptide-free cell culture media comprising at least 0.5 mg/L of a polyamine. The invention also relates to methods for expressing at least one protein in a medium comprising at least 0.5 mg/L of a polyamine and to methods for producing at least one virus in a medium comprising at least 0.5 mg/L of a polyamine.
摘要翻译：本发明涉及包含至少0.5mg / L多胺的无寡肽的细胞培养基和用于在所述无寡肽的细胞培养基中培养细胞的方法，其包含至少0.5mg / L 的多胺。本发明还涉及在包含至少0.5mg / L多胺的培养基中表达至少一种蛋白质的方法，以及在包含至少0.5mg / L多胺的培养基中生产至少一种病毒的方法。 p>

4. 发明申请

WO2012006591A1 METHOD OF PRODUCING RECOMBINANT HIGH MOLECULAR WEIGHT vWF IN CELL CULTURE 审中-公开
标题翻译：在细胞培养中生产重组高分子量vWF的方法
公开(公告)号：WO2012006591A1
公开(公告)日：2012-01-12
申请号：PCT/US2011/043455
申请日：2011-07-08
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A , GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang
发明人： GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang
IPC分类号： C07K14/755 , C12N5/00 , A61K38/36 , C12N5/10
CPC分类号： C07K14/755 , A61K9/0019 , A61K38/00 , A61K38/36 , A61K38/37 , C12N9/6489 , C12N2500/24 , C12N2511/00 , C12P21/00 , C12Y304/24087
摘要： Among other aspects, the present invention relates to cell culture conditions for producing high molecular weight vWF, in particular, highly multimericWF with a high specific activity and ADAMTS13 with a high specific activity. The cell culture conditions of the present invention can include, for example, a cell culture medium with an increased copper concentration and/or cell culture supernatant with a low ammonium (NH 4 + ) concentration. The present invention also provides methods for cultivating cells in the cell culture conditions to express high molecular weight vWF and rA13 having high specific activities.
摘要翻译：在其它方面，本发明涉及用于产生高分子量vWF，特别是具有高比活性的高度多聚体WF和具有高比活性的ADAMTS13的细胞培养条件。本发明的细胞培养条件可以包括例如具有增加的铜浓度的细胞培养基和/或具有低铵（NH 4 +）浓度的细胞培养物上清液。本发明还提供了在细胞培养条件下培养细胞以表达具有高比活性的高分子量vWF和rA13的方法。

5. 发明公开

EP2732020A1 VIRUS FILTRATION OF CELL CULTURE MEDIA 有权转让
标题翻译： VIRUSFILTRIERUNG细胞培养基
公开(公告)号：EP2732020A1
公开(公告)日：2014-05-21
申请号：EP13731015.7
申请日：2013-06-13
申请人： Baxter International Inc. , Baxter Healthcare SA , Mundt, Wolfgang , Mitterer, Artur , Reiter, Manfred , Hasslacher, Meinhard , Grillberger, Leopold , Kreil, Thomas
发明人： MUNDT, Wolfgang , MITTERER, Artur , REITER, Manfred , HASSLACHER, Meinhard , GRILLBERGER, Leopold , KREIL, Thomas
IPC分类号： C12M1/00 , A61L2/00 , C12M1/12 , C12N5/00
CPC分类号： C12N5/0602 , A61L2/0017 , C12M29/26 , C12M37/02
摘要： The invention relates to a method for removing a viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. The method comprises subjecting said preparation to filtration for at least about 24 hours through a virus filter having an effective pore size of maximum about 75 nm. Further, the invention relates to the use of a virus filter in filtration of at least about 24 hours, wherein the virus filter has an effective pore size of maximum about 75 nm for the removal of viral contaminant from a preparation, being a cell culture medium or at least a component of a cell culture medium. In some embodiments the filtration according to the invention operates at a volumetric capacity of at least about 2000 L/m2. Further, the invention relates to the use of a preparation, being a cell culture medium or at least a component of a cell culture medium obtainable according to method of the invention for cell culture; pharmaceutical, diagnostic and/or cosmetic preparations as well as in food preparations.

6. 发明申请

WO2008135230A1 TWO-STEP TEMPERATURE PROFILE FOR THE PROPAGATION OF VIRUSES 审中-公开
标题翻译：用于传播病毒的两步温度剖面
公开(公告)号：WO2008135230A1
公开(公告)日：2008-11-13
申请号：PCT/EP2008/003532
申请日：2008-04-30
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A. , REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang
发明人： REITER, Manfred , GRILLBERGER, Leopold , MUNDT, Wolfgang
IPC分类号： C12N7/00 , C12N7/02
CPC分类号： C12N7/00 , A61K39/12 , A61K39/145 , A61K2039/5252 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251 , C12N2770/24151 , C12N2770/36151 , Y02A50/394
摘要： The present invention provides a method for the production of a virus. The method includes providing a host cell that has been infected by the virus and cultivating the infected host cell at two different temperatures. The virus produced by the cultivation steps is subsequently collected. By using the dual temperature cultivation process, high titer and improved purity can be obtained.
摘要翻译：本发明提供一种生产病毒的方法。该方法包括提供已被病毒感染并在两个不同温度下培养感染的宿主细胞的宿主细胞。随后收集由培养步骤产生的病毒。通过使用双温培养方法，可以获得高滴定度和改善的纯度。

7. 发明申请

WO2006045438A1 ANIMAL PROTEIN-FREE MEDIA FOR CULTIVATION OF CELLS 审中-公开
标题翻译：用于培养细胞的动物蛋白质免费媒体
公开(公告)号：WO2006045438A1
公开(公告)日：2006-05-04
申请号：PCT/EP2005/010973
申请日：2005-10-12
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE S.A. , GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang , DORNER, Friedrich
发明人： GRILLBERGER, Leopold , REITER, Manfred , MUNDT, Wolfgang , DORNER, Friedrich
IPC分类号： C12N5/02 , C12N7/02 , C12N1/16 , C12N1/20
CPC分类号： C12N5/0043 , C12N5/0682 , C12N2500/46 , C12N2500/74 , C12N2500/76 , C12N2500/92
摘要： The present invention relates to animal protein-free cell culture media comprising polyamines and a plant- and/or yeast-derived hydrolysate. The invention also relates to animal protein-free culturing processes, wherein cells can be cultivated, propagated and passaged without adding supplementary animal proteins in the culture medium. These processes are useful in cultivating cells, such as recombinant cells or cells infected with a virus, and for producing biological products by cell culture processes.
摘要翻译：本发明涉及含有多胺和植物和/或酵母衍生的水解产物的无动物蛋白的细胞培养基。本发明还涉及无动物蛋白质的培养方法，其中细胞可培养，繁殖和传代，而不在培养基中添加补充动物蛋白。这些方法可用于培养细胞，例如重组细胞或感染病毒的细胞，以及通过细胞培养方法生产生物产物。

8. 发明申请

WO2009088713A1 SUBSTANTIALLY ANIMAL PROTEIN-FREE RECOMBINANT FURIN AND METHODS FOR PRODUCING SAME 审中-公开
标题翻译：重大动物蛋白质重组蛋白及其生产方法
公开(公告)号：WO2009088713A1
公开(公告)日：2009-07-16
申请号：PCT/US2008/087732
申请日：2008-12-19
申请人： BAXTER INTERNATIONAL INC. , BAXTER HEALTHCARE SA , PLAIMAUER, Barbara , VON FIRCKS, Simone , GRILLBERGER, Leopold , HASSLACHER, Meinhard , GEYER, Roland , MITTERER, Artur , REITER, Manfred
发明人： PLAIMAUER, Barbara , VON FIRCKS, Simone , GRILLBERGER, Leopold , HASSLACHER, Meinhard , GEYER, Roland , MITTERER, Artur , REITER, Manfred
IPC分类号： C12N9/64 , C07K14/755 , C12P21/02
CPC分类号： C12N9/6454
摘要： The present invention relates to recombinant furin (rFurin) and methods for producing rFurin. More specifically, the invention relates to substantially animal protein-free rFurin and methods for producing substantially animal protein-free rFurin.
摘要翻译：本发明涉及重组弗林蛋白酶（rFurin）和生产rFurin的方法。更具体地，本发明涉及基本上无动物蛋白的rFurin和用于生产基本上无动物蛋白的rFurin的方法。

9. 发明申请

WO2003050275A3 METHOD OF ISOLATION AND PURIFICATION OF TRYPSIN FROM PRONASE AND USE THEREOF 审中-公开
公开(公告)号：WO2003050275A3
公开(公告)日：2003-06-19
申请号：PCT/EP2002/014005
申请日：2002-12-10
申请人： BAXTER HEALTHCARE S.A. , MITTERER, Artur , TAUER, Christa , REITER, Manfred , MUNDT, Wolfgang
发明人： MITTERER, Artur , TAUER, Christa , REITER, Manfred , MUNDT, Wolfgang
IPC分类号： C07K1/22
摘要： The present invention provides methods of isolation and purification of Streptomyces griseus trypsin (SGT) from pronase in a single affinity chromatography step and uses of the purified SGT.

10. 发明申请

WO2011012725A1 METHOD OF PRODUCING A POLYPEPTIDE OR VIRUS OF INTEREST IN A CONTINUOUS CELL CULTURE 审中-公开
标题翻译：在连续细胞培养中生产多肽或感兴趣的病毒的方法
公开(公告)号：WO2011012725A1
公开(公告)日：2011-02-03
申请号：PCT/EP2010/061191
申请日：2010-08-02
申请人： BAXTER HEALTHCARE S.A. , BAXTER INTERNATIONAL INC. , GRILLBERGER, Leopold , REITER, Manfred , FLEISCHHANDERL, Daniel
发明人： GRILLBERGER, Leopold , REITER, Manfred , FLEISCHHANDERL, Daniel
IPC分类号： C12M3/00 , C12N5/00
CPC分类号： C12N7/00 , C12N9/6489 , C12N2710/00052 , C12P21/02 , Y02P20/52
摘要： Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d -1 , and a cell density of less than about 2X10 7 cell/mL. Also described herein is a method for producing a polypeptide and/or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and/or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d -1 , and a cell density of less than about 2X10 7 cell/mL; and recovering the polypeptide and/or virus of interest from medium of the cell culture system.
摘要翻译：本文描述的是一种恒化器样连续细胞培养系统，其结合了灌注开放系统和恒化器开放系统的某些优点，以改善哺乳动物细胞（例如遗传修饰的细胞，特别是在无血清或化学定义的培养基中）的培养。本文所述的连续培养系统包括在包含细胞保留装置的连续细胞培养系统中培养哺乳动物细胞，其中细胞培养系统的稀释速率（D）小于约2d-1，细胞密度小于约2X107细胞/ mL。本文还描述了在连续细胞培养物中产生感兴趣的多肽和/或病毒的方法，所述方法包括在连续细胞培养系统中培养表达所述多肽和/或感兴趣的病毒的哺乳动物细胞，其包含细胞保留装置其中细胞培养系统具有小于约2d-1的稀释倍数（D），小于约2×10 7细胞/ mL的细胞密度; 以及从细胞培养系统的培养基中回收目标多肽和/或病毒。

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