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1. 发明申请

WO2012050642A2 MICROCHIP CAPILLARY ELECTROPHORESIS ABSENT ELECTROKINETIC INJECTION 审中-公开
标题翻译：微芯片毛细血管电泳缺电动脉注射
公开(公告)号：WO2012050642A2
公开(公告)日：2012-04-19
申请号：PCT/US2011041032
申请日：2011-06-20
申请人： BATTELLE MEMORIAL INSTITUTE , SUN XUEFEI , KELLY RYAN T , TANG KEQI , SMITH RICHARD D
发明人： SUN XUEFEI , KELLY RYAN T , TANG KEQI , SMITH RICHARD D
IPC分类号： G01N27/26 , G01N27/447 , G01N35/10
CPC分类号： G01N27/44743 , G01N27/44791 , G01N30/461
摘要： Microchip capillary electrophoresis (CE) utilizing a sample injector based on a mechanical valve rather than electrokinetic injection can provide improved sample injections, enhanced capabilities, and can eliminate the need for changing the electric field in the separation channel to induce sample injection. In one instance CE electrodes continuously apply an electric field for CE separation along a separation channel. A sample channel is connected to the separation channel at an intersection and has a sample pressure that is greater than that which is present in the separation channel near the intersection. The sample channel does not have electrodes that apply voltages for electrokinetic injection. A sample injector in the sample channel or at the intersection comprises a mechanical valve to control sample injection from the sample channel to the separation channel.
摘要翻译：使用基于机械阀而非电动注射的样品注射器的微芯片毛细管电泳（CE）可以提供改进的样品注射，增强的能力，并且可以消除改变分离通道中的电场以诱导样品注射的需要。在一种情况下，CE电极沿分离通道连续施加用于CE分离的电场。样品通道在交叉点处连接到分离通道，并且样品压力大于在交叉点附近的分离通道中存在的样品压力。样品通道没有为电动注射施加电压的电极。样品通道或相交处的样品注射器包括机械阀以控制样品从样品通道到分离通道的注入。

2. 发明申请

WO2009042328A3 LOW PRESSURE ELECTROSPRAY IONIZATION SYSTEM AND PROCESS FOR EFFECTIVE TRANSMISSION OF IONS 审中-公开
标题翻译：低压电沉积离子系统和有效传输离子的方法
公开(公告)号：WO2009042328A3
公开(公告)日：2009-12-17
申请号：PCT/US2008074238
申请日：2008-08-25
申请人： BATTELLE MEMORIAL INSTITUTE , TANG KEQI , PAGE JASON S , KELLY RYAN T , SMITH RICHARD D
发明人： TANG KEQI , PAGE JASON S , KELLY RYAN T , SMITH RICHARD D
IPC分类号： H01J49/16 , H01J49/06
CPC分类号： H01J49/165 , H01J49/066
摘要： A system and method are disclosed that provide up to complete transmission of ions between coupled stages with low effective ion losses. A novel 'interfaceless' electrospray ionization system is further described that operates the electrospray at a reduced pressure such that standard electrospray sample solutions can be directly sprayed into an electrodynamic ion funnel which provides ion focusing and transmission of ions into a mass analyzer.
摘要翻译：公开了一种系统和方法，其提供在具有低有效离子损耗的耦合级之间的离子的完全传输。进一步描述了一种新颖的“无界面”电喷雾离子化系统，其在减压下操作电喷雾，使得标准电喷雾样品溶液可以直接喷射到提供离子聚焦和离子传输到质量分析器中的电动离子漏斗中。

3. 发明申请

WO2010051051A3 RADIAL ARRAYS OF NANO-ELECTROSPRAY IONIZATION EMITTERS AND METHODS OF FORMING ELECTROSPRAYS 审中-公开
标题翻译：纳米电子放电发射体的辐射阵列及形成电沉积的方法
公开(公告)号：WO2010051051A3
公开(公告)日：2010-09-02
申请号：PCT/US2009035817
申请日：2009-03-03
申请人： BATTELLE MEMORIAL INSTITUTE , KELLY RYAN , TANG KEQI , SMITH RICHARD D
发明人： KELLY RYAN , TANG KEQI , SMITH RICHARD D
IPC分类号： H01J49/16
CPC分类号： H01J49/167
摘要： Electrospray ionization emitter arrays, as well as methods for forming electrosprays, are described. The arrays are characterized by a radial configuration of three or more nano- electrospray ionization emitters without an extractor electrode. The methods are characterized by distributing fluid flow of the liquid sample among three or more nano- electrospray ionization emitters, forming an electrospray at outlets of the emitters without utilizing an extractor electrode, and directing the electrosprays into an entrance to a mass spectrometry device. Each of the nano-electrospray ionization emitters can have a discrete channel for fluid flow. The nano-electrospray ionization emitters are circularly arranged such that each is shielded substantially equally from an electrospray-inducing electric field.
摘要翻译：描述了电喷雾电离发射器阵列，以及用于形成电喷雾的方法。阵列的特征在于没有提取器电极的三个或更多个纳米电喷雾电离发射器的径向构型。所述方法的特征在于在三个或更多个纳米电喷雾电离发射器之间分配液体样品的流体流动，在不使用提取器电极的情况下在发射器的出口处形成电喷雾，并将电喷雾引导到质谱装置的入口。每个纳米电喷雾电离发射器可以具有用于流体流动的离散通道。纳米电喷雾电离发射器是圆形布置的，使得每个发射器基本上等同于电喷雾诱导电场屏蔽。

4. 发明申请

WO2005045876A3 IMPROVED HIGH PERFORMANCE ION MOBILITY SPECTROMETRY USING HOURGLASS ELECTRODYNAMIC FUNNEL AND INTERNAL ION FUNNEL 审中-公开
标题翻译：改进的高性能离子电离光谱使用HOURGLASS ELECTRODYMMIC FUNNEL和内部离子FUNNEL
公开(公告)号：WO2005045876A3
公开(公告)日：2006-05-26
申请号：PCT/US2004029181
申请日：2004-09-07
申请人： BATTELLE MEMORIAL INSTITUTE , SMITH RICHARD D , TANG KEQI , SHVARTSBURG ALEXANDRE A
发明人： SMITH RICHARD D , TANG KEQI , SHVARTSBURG ALEXANDRE A
IPC分类号： H01J49/04 , G01N27/64 , H01J49/02 , H01J49/06 , H01J49/16 , H01J49/40 , H01J49/42
CPC分类号： H01J49/066 , G01N27/622 , H01J49/40
摘要： A method and apparatus enabling increased sensitivity in ion mobility spectrometry/mass spectrometry instruments which substantially reduces or eliminates the loss of ions in ion mobility spectrometer drift tubes utilizing a device for transmitting ions from an ion source which allows the transmission of ions without significant delay to an hourglass electrodynamic ion funnel at the entrance to the drift tube and/or an internal ion funnel at the exit of the drift tube. An hourglass electrodynamic funnel is formed of at least an entry element, a center element, and an exit element, wherein the aperture of the center element is smaller than the aperture of the entry element and the aperture of the exit elements. Ions generated in a relatively high pressure region by an ion source at the exterior of the hourglass electrodynamic funnel are transmitted to a relatively low pressure region at the entrance of the hourglass funnel through a conductance limiting orifice. Alternating and direct electrical potentials are applied to the elements of the hourglass electrodynamic funnel thereby drawing ions into and through the hourglass electrodynamic funnel thereby introducing relatively large quantities of ions into the drift tube while maintaining the gas pressure and composition at the interior of the drift tube as distinct from those at the entrance of the electrodynamic funnel and allowing a positive gas pressure to be maintained within the drift tube, if desired. An internal ion funnel is provided within the drift tube and is positioned at the exit of said drift tube. The advantage of the internal ion funnel is that ions that are dispersed away from the exit aperture within the drift tube, such as those that are typically lost in conventional drift tubes to any subsequent analysis or measurement, are instead directed through the exit of the drift tube, vastly increasing the amount of ions exiting the drift tube.
摘要翻译：一种能够提高离子迁移光谱/质谱仪器灵敏度的方法和装置，其基本上减少或消除了离子迁移谱仪漂移管中的离子损失，其利用离子源传输离子的装置，离子源允许离子传输而不会显着延迟漂移管入口处的沙漏电动力离子漏斗和/或漂移管出口处的内部离子漏斗。沙漏电动漏斗由至少一个入口元件，中心元件和出口元件形成，其中中心元件的孔径小于入口元件的孔径和出口元件的孔径。在沙漏电动漏斗的外部由离子源在较高压力区域产生的离子通过电导限制孔传输到沙漏漏斗入口处的较低压力区域。将交替和直接的电势施加到沙漏电动力学漏斗的元件，从而将离子吸入和穿过沙漏电动力学漏斗，从而将相对大量的离子引入漂移管中，同时保持气体压力和组成在漂移管的内部与电动漏斗的入口处的那些不同，并且如果需要，允许在漂移管内保持正的气体压力。内部离子漏斗设置在漂移管内并位于所述漂移管的出口处。内部离子漏斗的优点是离散在漂移管内的出口孔的离子，例如通常在常规漂移管中丢失到任何后续分析或测量中的离子，而是通过漂移出口管，大大增加离开漂移管的离子的量。

5. 发明申请

WO2007092227A2 MONOLITHIC ELECTROSPRAY IONIZATION EMITTERS AND METHODS OF MAKING SAME 审中-公开
标题翻译：单晶电沉积离子发射体及其制备方法
公开(公告)号：WO2007092227A2
公开(公告)日：2007-08-16
申请号：PCT/US2007002657
申请日：2007-01-29
申请人： BATTELLE MEMORIAL INSTITUTE , TANG KEQI , PAGE JASON S , LUO QUANZHOU , SMITH RICHARD D
发明人： TANG KEQI , PAGE JASON S , LUO QUANZHOU , SMITH RICHARD D
CPC分类号： H01J49/167 , G01N30/7266
摘要： Electrospray ionization (ESI) apparatuses and methods of making the same are disclosed. In one embodiment, an ESI emitter comprises a capillary tube filled with a porous monolithic material, wherein the end of the emitter is tapered and the tip of the tapered end comprises a protrusion of the porous monolithic material. One embodiment of the method for making ESI emitters comprises filling a capillary tube with a porous monolithic material and tapering the end of the capillary tube, thereby exposing a portion of the porous material. The exposed portion of the porous material at the tapered end, rather than the capillary tube itself, forms the tip of the ESI emitter.
摘要翻译：公开了电喷雾离子化（ESI）装置及其制备方法。在一个实施例中，ESI发射器包括填充有多孔整体材料的毛细管，其中发射器的端部是锥形的，锥形末端包括多孔整体材料的突起。用于制备ESI发射器的方法的一个实施例包括用多孔整体材料填充毛细管并使毛细管的端部逐渐变细，由此暴露多孔材料的一部分。锥形端部的多孔材料的暴露部分而不是毛细管本身形成ESI发射体的尖端。

6. 发明申请

WO2006083262A3 METHOD FOR ENHANCED ACCURACY IN PREDICTING PEPTIDES ELUTION TIME USING LIQUID SEPARATIONS OR CHROMATOGRAPHY 审中-公开
标题翻译：使用液相分离或色谱法预测肽时间的增强精度的方法
公开(公告)号：WO2006083262A3
公开(公告)日：2007-01-11
申请号：PCT/US2005015604
申请日：2005-05-05
申请人： BATTELLE MEMORIAL INSTITUTE , PETRITIS KONSTANTINOS , KANGAS LARS J , ANDERSON GORDON A , SMITH RICHARD D
发明人： PETRITIS KONSTANTINOS , KANGAS LARS J , ANDERSON GORDON A , SMITH RICHARD D
IPC分类号： B01D15/08 , C07K1/16 , G01N33/68 , G06F19/00
CPC分类号： C07K1/16 , G01N30/8693 , G01N33/6803 , G01N33/6806 , G01N2030/8831 , G06F19/24
摘要： A method for predicting the elution time of a peptide in chromatographic and electrophoretic separations by first providing a data set of known elution times of known peptides, then creating a plurality of vectors, each vector having a plurality of dimensions, and each dimension representing positional information about at least a portion of the amino acids present in the known peptides. A hypothetical vector is then created by assigning dimensional values for at least one hypothetical peptide, and a predicted elution time for the hypothetical vector is created by performing at least one multivariate regression fitting the hypothetical peptide to the plurality of vectors. Preferably, the multivariate regression is accomplished by the use of an artificial neural network and the elution times are first normalized using linear regression.
摘要翻译：一种用于通过首先提供已知肽的已知洗脱时间的数据集，然后创建多个向量，每个向量具有多个维度，并且每个维度表示位置信息的方法，用于预测肽在色谱和电泳分离中的洗脱时间约至少一部分已知肽中存在的氨基酸。然后通过为至少一个假想肽分配尺寸值来产生假想载体，并且通过对多个载体进行拟合假想肽的至少一个多变量回归而产生假设载体的预测洗脱时间。优选地，通过使用人造神经网络实现多元回归，并且首先使用线性回归归一化洗脱时间。

7. 发明申请

WO2009140390A3 SERUM MARKERS FOR TYPE II DIABETES MELLITUS 审中-公开
标题翻译： II型糖尿病血清标记物
公开(公告)号：WO2009140390A3
公开(公告)日：2010-01-28
申请号：PCT/US2009043794
申请日：2009-05-13
申请人： BATTELLE MEMORIAL INSTITUTE , METZ THOMAS O , QIAN WEI-JUN , JACOBS JON M , POLPITIYA ASHOKA D , CAMP DAVID G , SMITH RICHARD D
发明人： METZ THOMAS O , QIAN WEI-JUN , JACOBS JON M , POLPITIYA ASHOKA D , CAMP DAVID G , SMITH RICHARD D
IPC分类号： G01N33/53 , C40B40/10
CPC分类号： G01N33/6893 , G01N2800/042
摘要： A method for identifying persons with increased risk of developing type 2 diabetes mellitus, or having type II diabetes mellitus, utilizing selected biomarkers described herein either alone or in combination. The present disclosure allows for broad based, reliable, screening of large population bases and provides other advantages, including the formulation of effective strategies for characterizing, archiving, and contrasting data from multiple sample types under varying conditions. Also provided are arrays and kits that can be used to perform such methods.
摘要翻译：一种利用本文所述的选择的生物标志物单独或组合地鉴定发生2型糖尿病或具有II型糖尿病风险增加的人的方法。本公开允许广泛的，可靠的大量人口基因的筛选，并提供其他优点，包括在不同条件下制定用于表征，归档和对比来自多种样品类型的数据的有效策略。还提供了可用于执行这些方法的数组和工具包。

8. 发明申请

WO2008128029A3 SOLUTION FRAGMENTATION SYSTEMS AND PROCESSES FOR PROTEOMICS ANALYSIS 审中-公开
标题翻译：解决方案碎片系统和蛋白质组分析过程
公开(公告)号：WO2008128029A3
公开(公告)日：2008-12-11
申请号：PCT/US2008060011
申请日：2008-04-11
申请人： BATTELLE MEMORIAL INSTITUTE , PETRITIS KONSTANTINOS , SMITH RICHARD D
发明人： PETRITIS KONSTANTINOS , SMITH RICHARD D
IPC分类号： G01N33/68
CPC分类号： G01N33/6803 , G01N33/6842
摘要： A solution-phase digestion process is described. Intact proteins are digested to obtain parent peptides, which are separated and subsequently mass analyzed. Individual parent peptides are digested to obtain daughter peptides, which are also subsequently mass analyzed. Accurate mass data obtained from mass analysis of both parent and daughter peptides are correlated with separations data obtained during separation of the parent peptides to provide peptide identification. The process is expected to provide unique peptides by which to identify intact proteins in a sample without need for MS/MS gas-phase fragmentation.
摘要翻译：描述了溶液相消化过程。消化完整的蛋白质以获得亲本肽，将其分离并随后进行质量分析。单个亲本肽被消化以获得子体肽，其随后也被质量分析。从母体和子体肽的质量分析获得的准确质量数据与在分离母体肽期间获得的分离数据相关以提供肽鉴定。该过程预计将提供独特的肽，通过该肽可以识别样品中的完整蛋白质，而无需MS / MS气相碎裂。

9. 发明申请

WO2008112351A3 METHOD OF MULTIPLEXED ANALYSIS USING ION MOBILITY SPECTROMETER 审中-公开
标题翻译：使用离子移动光谱仪进行多重分析的方法
公开(公告)号：WO2008112351A3
公开(公告)日：2009-09-24
申请号：PCT/US2008052565
申请日：2008-01-31
申请人： BATTELLE MEMORIAL INSTITUTE , BELOV MIKHAIL E , SMITH RICHARD D
发明人： BELOV MIKHAIL E , SMITH RICHARD D
IPC分类号： H01J49/00
CPC分类号： H01J49/0031 , G01N27/622
摘要： A method for analyzing analytes from a sample introduced into a Spectrometer by generating a pseudo random sequence of a modulation bins, organizing each modulation bin as a series of submodulation bins, thereby forming an extended pseudo random sequence of submodulation bins, releasing the analytes in a series of analyte packets into a Spectrometer, thereby generating an unknown original ion signal vector, detecting the analytes at a detector, and characterizing the sample using the plurality of analyte signal subvectors. The method is advantggeously applied to an Ion Mobility Spectrometer, and an Ion Mobility Spectrometer interfaced with a Time of Flight Mass Spectrometer.
摘要翻译：一种用于通过产生调制箱的伪随机序列来分析从引入到光谱仪中的样品的分析物的方法，将每个调制箱组织成一系列的子调制箱，由此形成扩频的伪随机序列的子调制箱，将分析物在一系列分析物分组进入光谱仪，从而产生未知的原始离子信号矢量，检测检测器处的分析物，并使用多个分析物信号子矢量表征样品。该方法有利于应用于离子迁移光谱仪和离子迁移光谱仪与飞行时间质谱仪接口。

10. 发明申请

WO2004057342A3 METHOD FOR ENHANCED ACCURACY IN PREDICTING THE ELUTION TIMES OF PEPTIDES USING LIQUID SEPARATIONS OR CHROMATOGRAPHY 审中-公开
标题翻译：使用液体分离或色谱法预测肽的时间的增强精度的方法
公开(公告)号：WO2004057342A3
公开(公告)日：2004-10-07
申请号：PCT/US0340949
申请日：2003-12-18
申请人： BATTELLE MEMORIAL INSTITUTE , KANGAS LARS J , AUBERRY KENNETH J , ANDERSON GORDON A , SMITH RICHARD D
发明人： KANGAS LARS J , AUBERRY KENNETH J , ANDERSON GORDON A , SMITH RICHARD D
IPC分类号： G01N33/68 , G06F19/00
CPC分类号： G01N30/8693 , G01N33/6806 , G01N2030/8831 , G06F19/24
摘要： A method for predicting the elution time of a peptide in chromatographic and electrophoretic separations by first providing a data set of known elution times of known peptides, then creating a plurality of vectors, each vector having a plurality of dimensions, and each dimension representing the elution time of amino acids present in each of these known peptides from the data set. The elution time of any protein is then be predicted by first creating a vector by assigning dimensional values for the elution time of amino acids of at least one hypothetical peptide and then calculating a predicted elution time for the vector by performing a multivariate regression of the dimensional values of the hypothetical peptide using the dimensional values of the known peptides. Preferably, the multivariate regression is accomplished by the use of an artificial neural network and the elution times are first normalized using a transfer function.
摘要翻译：一种通过首先提供已知肽的已知洗脱时间的数据集，然后产生多个载体，每个载体具有多个维度，并且每个维度代表洗脱的方法，用于预测肽在色谱和电泳分离中的洗脱时间的方法来自数据集的这些已知肽各自存在的氨基酸的时间。然后通过首先通过分配至少一个假想肽的氨基酸的洗脱时间的维度值来产生载体，然后通过执行尺寸的多元回归来计算载体的预测洗脱时间来预测任何蛋白质的洗脱时间使用已知肽的尺寸值的假想肽的值。优选地，通过使用人造神经网络实现多元回归，并且首先使用传递函数归一化洗脱时间。

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