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    • 5. 发明申请
    • MAP-BASED GENOME MINING METHOD FOR IDENTIFYING GENE REGULATORY LOCI
    • 基于MAP的基因组挖掘方法,用于鉴定基因调控位点
    • WO0212551A3
    • 2003-10-02
    • PCT/CA0101028
    • 2001-07-17
    • CANADA NATURAL RESOURCESDNA LANDMARKS INCCASTONGUAY YVESO'DONOUGHUE LOUISE SLANDRY BENOIT SLABERGE SERGEMONROY ANTONIO FVEZINA LOUIS P
    • CASTONGUAY YVESO'DONOUGHUE LOUISE SLANDRY BENOIT SLABERGE SERGEMONROY ANTONIO FVEZINA LOUIS P
    • A01H1/00C12N15/09C12Q1/68
    • C12Q1/6897C12Q1/6809
    • The invention pertains to a method for identifying one or more regions within a genome of an organism of interest that mediate the expression of one or more genes of interest. The method comprises identifying a first and a second organism of interest, the first organism of interest is characterized by exhibiting a measurable response to an environmental stimulus, or otherwise exhibiting a phenotype associated with differential gene expression associated with a process of interest. The second organism of interest is characterized by lacking or not exhibit as strong a response to the stimulus as that observed within the first organism of interest, or it exhibits a different phenotype compared with that of the first organism of interest, wherein the different phenotype is associated with the process of interest, or it exhibits a phenotype of interest that segregates when compared with the first organism of interest, or a combination thereof. The first and second organisms of interest are crossed to produce a population of segregated progeny and RNA is extracted from each of the segregated progeny. The level of gene expression for one or more genes of interest that are associated with the response to an environmental stimulus, or process of interest is quantified.
    • 本发明涉及用于鉴定介导一种或多种感兴趣基因表达的感兴趣生物体的基因组内的一个或多个区域的方法。 该方法包括鉴定感兴趣的第一和第二生物体,所述第一感兴趣的生物体的特征在于显示对环境刺激的可测量的反应,或者表现出与感兴趣的过程相关的差异基因表达相关的表型。 第二有兴趣的生物体的特征在于缺乏或不表现出与目标第一生物体内观察到的对刺激的强烈反应,或者与第一感兴趣的生物体相比表现出不同的表型,其中不同的表型是 或者与感兴趣的第一生物体相比,其表现出分离的感兴趣的表型,或其组合。 将感兴趣的第一和第二生物杂交以产生分离后代的群体,并且从每个分离的后代中提取RNA。 与感兴趣的环境刺激或感兴趣过程相关的一种或多种感兴趣的基因的基因表达水平被量化。