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1. 发明申请

US20210163975A1 OILS, LIPIDS AND FATTY ACIDS PRODUCED IN TRANSGENIC BRASSICA PLANT 有权
公开(公告)号：US20210163975A1
公开(公告)日：2021-06-03
申请号：US17085135
申请日：2020-10-30
申请人： BASF PLANT SCIENCE GMBH
发明人： Petra Cirpus , Joerg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

2. 发明申请

US20160369290A1 METHOD FOR PRODUCING POLYUNSATURATED FATTY ACIDS 审中-公开
标题翻译：生产聚氨酯脂肪酸的方法
公开(公告)号：US20160369290A1
公开(公告)日：2016-12-22
申请号：US15256914
申请日：2016-09-06
申请人： BASF Plant Science GmbH
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： C12N15/82 , C12P7/64 , C12N15/52 , C12N9/02
CPC分类号： C12N15/8247 , C12N9/0071 , C12N9/0083 , C12N9/1029 , C12N15/52 , C12P7/6427 , C12Y114/19
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.
摘要翻译：本发明涉及在转基因植物中生产二十烷酸，二十二烷酸和/或二十二酸的方法。根据所述方法，向植物提供编码具有Δ6去饱和酶活性的多肽的至少一种核酸序列，至少一种编码具有Δ6延伸酶活性的多肽的核酸序列，至少一种编码具有Δ5去饱和酶活性的多肽和编码具有Δ5延伸酶活性的多肽的至少一种核酸序列，编码具有相对于生物体中的核酸序列修饰的Δ5延伸酶活性的多肽的核酸序列序列来自该序列，使得其适于在至少一种类型的植物中的密码子使用。为了生产二十二碳六烯酸，还将至少一种编码具有Δ4去饱和酶活性的多肽的核酸序列引入植物中。

3. 发明授权

US10533182B2 Oils, lipids and fatty acids produced in transgenic brassica plant 有权
公开(公告)号：US10533182B2
公开(公告)日：2020-01-14
申请号：US16371451
申请日：2019-04-01
申请人： BASF Plant Science GmbH
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： A61K31/20 , C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

4. 发明授权

US10301638B2 Oils, lipids and fatty acids produced in transgenic Brassica plant 有权
公开(公告)号：US10301638B2
公开(公告)日：2019-05-28
申请号：US15256914
申请日：2016-09-06
申请人： BASF Plant Science GmbH
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： A61K31/20 , C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentaenoic acid, docosapentaenoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

5. 发明申请

US20220213497A1 Oils, Lipids and Fatty Acids Produced in Transgenic Brassica Plant 有权
公开(公告)号：US20220213497A1
公开(公告)日：2022-07-07
申请号：US17571091
申请日：2022-01-07
申请人： BASF Plant Science GMBH
发明人： Petra Cirpus , Jorg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/6427
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypetide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

6. 发明授权

US10533183B2 Oils, lipids and fatty acids produced in transgenic Brassica plant 有权
公开(公告)号：US10533183B2
公开(公告)日：2020-01-14
申请号：US16371837
申请日：2019-04-01
申请人： BASF Plant Science GmbH
发明人： Petra Cirpus , Jörg Bauer , Xiao Qiu , Guohai Wu , Bifang Cheng , Martin Truksa , Tom Wetjen
IPC分类号： A61K31/20 , C12N15/82 , C12N9/02 , C12N9/10 , C12N15/52 , C12P7/64
摘要： The invention relates to a method for producing eicosapentanoic acid, docosapentanoic acid and/or docohexanoic acid in transgenic plants. According to said method, the plant is provided with at least one nucleic acid sequence coding for a polypeptide with a Δ6 desaturase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ6 elongase activity, at least one nucleic acid sequence coding for a polypeptide with a Δ5 desaturase activity, and at least one nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity, the nucleic acid sequence coding for a polypeptide with a Δ5 elongase activity being modified in relation to the nucleic acid sequence in the organism from which the sequence originates, such that it is adapted to the codon use in at least one type of plant. For the production of docosahexanoic acid, at least one nucleic acid sequence coding for a polypeptide with a Δ4 desaturase activity is also introduced into the plant.

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