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1. 发明申请

WO2013027160A1 METHODS OF INCREASING PROTEIN, OIL, AND/OR AMINO ACID CONTENT IN PLANT 审中-公开
标题翻译：增加植物蛋白质，油和/或氨基酸含量的方法
公开(公告)号：WO2013027160A1
公开(公告)日：2013-02-28
申请号：PCT/IB2012/054175
申请日：2012-08-16
申请人： BASF PLANT SCIENCE COMPANY GMBH , GUAN, Hanping , HÄRTEL, Heiko , COCCIOLONE, Suzy , BASF (CHINA) COMPANY LIMITED
发明人： GUAN, Hanping , HÄRTEL, Heiko , COCCIOLONE, Suzy
IPC分类号： C12N15/82 , C12N5/14 , C12N9/12 , C12N9/00 , A01H5/00 , A01H1/02
CPC分类号： C12N15/8251 , A23K20/142 , A23K20/147 , A23K20/158 , A23L7/10 , C12N9/1051 , C12N15/8247
摘要： The invention provides methods for preparing a plant, plant cell, or plant part with increased content in one or more of proteins, oil, or one or more amino acids relative to a corresponding wild-type plant, plant cell, or plant part. The invention also provides transgenic plants comprising multiple genes of interest including a nucleic acid molecule encoding a polypeptide having pyruvate kinase activity and a nucleic acid molecule encoding a trehalose-6-phosphate synthase (TPS) homolog. Expression cassettes for achieving such gene expression manipulation, as well as recombinant constructs, vectors and plants, plant cells, or plant parts comprising the same are also provided.
摘要翻译：本发明提供了相对于相应的野生型植物，植物细胞或植物部分制备在一种或多种蛋白质，油或一种或多种氨基酸中具有增加的含量的植物，植物细胞或植物部分的方法。本发明还提供包含多个感兴趣的基因的转基因植物，包括编码具有丙酮酸激酶活性的多肽的核酸分子和编码海藻糖-6-磷酸合成酶（TPS）同源物的核酸分子。还提供了用于实现这种基因表达操纵的表达盒，以及重组构建体，载体和植物，植物细胞或包含其的植物部分。

2. 发明申请

WO2012143805A1 METHODS OF MODIFYING LIGNIN BIOSYNTHESIS AND IMPROVING DIGESTIBILITY 审中-公开
标题翻译：修饰木质素生物合成和改善可溶性的方法
公开(公告)号：WO2012143805A1
公开(公告)日：2012-10-26
申请号：PCT/IB2012/051503
申请日：2012-03-29
申请人： BASF PLANT SCIENCE COMPANY GMBH , SEO, Beomseok , GUAN, Hanping , REN, Peifeng , DEPPONG, David , FU, Huihua , BASF (CHINA) COMPANY LIMITED
发明人： SEO, Beomseok , GUAN, Hanping , REN, Peifeng , DEPPONG, David , FU, Huihua
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/1011 , C12N15/8255 , C12Y201/01104
摘要： Provided are methods for increasing digestibility and/or crude protein of a plant by modifying lignin biosynthesis of the plant. The methods involve the manipulation of the expression level of genes in the lignin biosynthesis pathway by directly reducing the expression of genes using miRNA or using regulation of transcription factors. Expression cassettes for achieving such gene expression manipulation and transgenic plant cells and plants comprising the constructs and cassettes are also provided. Transcription regulating nucleotide sequences for use in the expression cassettes have also been developed as well as methods of using these sequences and cassettes.
摘要翻译：提供了通过改变植物的木质素生物合成来增加植物的消化率和/或粗蛋白质的方法。该方法涉及通过使用miRNA直接降低基因的表达或使用转录因子的调节来操纵木质素生物合成途径中基因的表达水平。还提供了用于实现这种基因表达操纵的表达盒和包含构建体和盒的转基因植物细胞和植物。还开发了用于表达盒的转录调节核苷酸序列以及使用这些序列和盒的方法。

3. 发明申请

WO2002079410A3 GLUCAN CHAIN LENGTH DOMAINS 审中-公开
公开(公告)号：WO2002079410A3
公开(公告)日：2002-10-10
申请号：PCT/US2002/009574
申请日：2002-03-29
申请人： BASF PLANT SCIENCE GMBH , COMMURI, Padma , KEELING, Peter, L. , RAMIREZ, Nona , McKEAN, Angela , GAO, Zhong , GUAN, Hanping
发明人： COMMURI, Padma , KEELING, Peter, L. , RAMIREZ, Nona , McKEAN, Angela , GAO, Zhong , GUAN, Hanping
IPC分类号： C12N5/04
摘要： The invention relates to a method for changing the glucan chain lengths using fusion protein domains of various starch synthase enzymes in any starch or starch granule producing organism. The invention relates to identification of a Gl ucan ASS ociation domain (herein after referred to as "GLASS" domain) of granule bound starch synthase (GBSS) used in combination with any other GLY cosyl Tr ransferase domain otherwise referred to as pfam00534-catalytic domain (herein after referred to as "GLYTR" domain) of one or more of any of the other starch synthase enzymes. The invention relates to identifying and using the new and surprising discovery that starch synthases are composed of at least two distinct functional domains herein after labelel as "GLASS" and "GLYTR". More specifically, this invention relates to the genetic constructs that encode the fusions of the above domains and to the plants transformed with said constructs. The method of invention can thus be used in particular to provide a modified profile of starch granule associated starch synthase (SS) enzymes and by which modified glucan chain lengths of amylopectin and hence, modified starches and or complexes will be generated. This can be done in any organism and more particularly and plant that stores or synthesizes starch in any of its parts, such as potato, sweet potato, cassava, pea, taro, banana, yam and cereal crops such as rice, maize, wheat, barley, oats, and sorghum.

4. 发明申请

WO2005104822A1 TRANSGENIC MAIZE PLANTS HAVING ENHANCED NUTRITIONAL QUALITIES 审中-公开
标题翻译：具有增强营养品质的转基因植物
公开(公告)号：WO2005104822A1
公开(公告)日：2005-11-10
申请号：PCT/US2005/013936
申请日：2005-04-21
申请人： BASF PLANT SCIENCE GMBH , GUAN, Hanping , WETTERBERG, Deborah , MCKEAN, Angela, L. , KEELING, Peter, L.
发明人： GUAN, Hanping , WETTERBERG, Deborah , MCKEAN, Angela, L. , KEELING, Peter, L.
IPC分类号： A01H5/00
CPC分类号： C12N15/8247 , C12N9/1241 , C12N15/8251 , C12N15/8261 , Y02A40/146
摘要： The invention provides transgenic corn seed, which expresses a gene encoding a double mutant of the E. coli glgC gene in endosperm plastids, wherein the mutant protein has a proline to aspartic acid substitution at amino acid 295 and a glutamic acid to lysine substitution at amino acid 296. The transgenic corn seed of the invention is characterized by enhanced levels of a number of amino acids and oil, when compared to isogenic corn seed, which does not express the transgene in an endosperm plastid. However, the amount of starch in the transgenic corn seed of the invention is decreased or unchanged when compared to the amount of starch in the isogenic control corn seed.
摘要翻译：本发明提供了转基因玉米种子，其表达编码胚乳质体中大肠杆菌glgC基因的双突变体的基因，其中突变蛋白质具有在氨基酸295处天冬氨酸取代的脯氨酸，在氨基酸处具有谷氨酸至赖氨酸取代酸296.当与在胚乳质体中不表达转基因的等基因玉米种子相比，本发明的转基因玉米种子的特征在于增加多种氨基酸和油的含量。然而，与同基因对照玉米种子中的淀粉的量相比，本发明的转基因玉米种子中的淀粉的量减少或不变。

5. 发明申请

WO2009150170A1 METHODS IN INCREASING GRAIN VALUE BY IMPROVING GRAIN YIELD AND QUALITY 审中-公开
标题翻译：通过提高谷物质量和质量来提高谷物价值的方法
公开(公告)号：WO2009150170A1
公开(公告)日：2009-12-17
申请号：PCT/EP2009/057151
申请日：2009-06-10
申请人： BASF PLANT SCIENCE GMBH , GUAN, Hanping , SEO, Beomseok , HARDIN, Shane
发明人： GUAN, Hanping , SEO, Beomseok , HARDIN, Shane
IPC分类号： C12N15/82
CPC分类号： C12N15/8262 , C12N9/1025
摘要： The invention provides a transgenic plant, which expresses a transgene encoding a citrate synthase (CS) wherein the transgenic plant seed of the invention is characterized by increased yield and/or enhanced levels of protein, essential amino acids or oil, when compared to an isoline that does not express the transgene; and also provides methods of producing transgenic plants with economically relevant traits and provides expression vectors comprising polynucleotides encoding Citrate Synthase.
摘要翻译：本发明提供了一种转基因植物，其表达编码柠檬酸合酶（CS）的转基因，其中本发明的转基因植物种子的特征在于与等位基因相比增加的产量和/或增加的蛋白质，必需氨基酸或油的水平不表达转基因; 并且还提供生产具有经济相关性状的转基因植物的方法，并提供包含编码柠檬酸盐合酶的多核苷酸的表达载体。

6. 发明申请

WO2008125528A2 POLYNUCLEOTIDES FOR REGULATION OF HIGH LEVEL TISSUE-PREFERRED EXPRESSION IN CROP PLANTS 审中-公开
标题翻译：用于调控高等植物组织中多核苷酸优先表达的作物
公开(公告)号：WO2008125528A2
公开(公告)日：2008-10-23
申请号：PCT/EP2008/054130
申请日：2008-04-07
申请人： BASF PLANT SCIENCE GMBH , KAFER, Christopher , GUAN, Hanping
发明人： KAFER, Christopher , GUAN, Hanping
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N15/8234
摘要： This invention provides polynucleotides regulating high level tissue-preferred expression. Compositions comprising the polynucleotides include DNA constructs useful for plant transformation and plants transformed with such DNA constructs. Further provided are methods for the expression of transgenes in plants using the tissue-preferred regulatory elements.
摘要翻译：本发明提供调节高水平组织优选表达的多核苷酸。包含多核苷酸的组合物包括用于植物转化的DNA构建体和用这种DNA构建体转化的植物。还提供了使用组织优选调控元件在植物中表达转基因的方法。

7. 发明申请

WO2002079410A2 GLUCAN CHAIN LENGTH DOMAINS 审中-公开
标题翻译：葡萄牙链长度域
公开(公告)号：WO2002079410A2
公开(公告)日：2002-10-10
申请号：PCT/US2002/009574
申请日：2002-03-29
申请人： BASF PLANT SCIENCE GMBH , COMMURI, Padma , KEELING, Peter, L. , RAMIREZ, Nona , McKEAN, Angela , GAO, Zhong , GUAN, Hanping
发明人： COMMURI, Padma , KEELING, Peter, L. , RAMIREZ, Nona , McKEAN, Angela , GAO, Zhong , GUAN, Hanping
IPC分类号： C12N
CPC分类号： C12N9/1051 , C07K2319/00 , C12N15/8245 , C12N15/8257
摘要： The invention relates to a method for changing the glucan chain lengths using fusion protein domains of various starch synthase enzymes in any starch or starch granule producing organism. The invention relates to identification of a Gl ucan ASS ociation domain (herein after referred to as "GLASS" domain) of granule bound starch synthase (GBSS) used in combination with any other GLY cosyl Tr ransferase domain otherwise referred to as pfam00534-catalytic domain (herein after referred to as "GLYTR" domain) of one or more of any of the other starch synthase enzymes. The invention relates to identifying and using the new and surprising discovery that starch synthases are composed of at least two distinct functional domains herein after labelel as "GLASS" and "GLYTR". More specifically, this invention relates to the genetic constructs that encode the fusions of the above domains and to the plants transformed with said constructs. The method of invention can thus be used in particular to provide a modified profile of starch granule associated starch synthase (SS) enzymes and by which modified glucan chain lengths of amylopectin and hence, modified starches and or complexes will be generated. This can be done in any organism and more particularly and plant that stores or synthesizes starch in any of its parts, such as potato, sweet potato, cassava, pea, taro, banana, yam and cereal crops such as rice, maize, wheat, barley, oats, and sorghum.
摘要翻译：本发明涉及使用任何淀粉或淀粉颗粒生产生物体中各种淀粉合成酶的融合蛋白结构域来改变葡聚糖链长度的方法。本发明涉及用于与任意的组合使用的颗粒结合的淀粉合成酶（GBSS）的ucuco结构域（以下称为“玻璃”域）的鉴定另一种其它一种或多种任何其它淀粉合成酶（称为“GLYTR”结构域）的pfam00534-催化结构域（以下简称“GLYTR”结构域）之间的GLY 酶。本发明涉及鉴定和使用新的和令人惊奇的发现，淀粉合成酶由本文中的至少两个不同的功能结构域组成，作为“玻璃”和“GLYTR”。更具体地，本发明涉及编码上述结构域的融合物和用所述构建体转化的植物的遗传构建体。因此，本发明的方法可以特别用于提供淀粉颗粒相关淀粉合成酶（SS）酶的改性分布，由此将产生修饰的葡聚糖链长度的支链淀粉，因此将改变淀粉和/或复合物。这可以在任何生物体，更具体地和在其任何部分存储或合成淀粉的植物中进行，例如马铃薯，甘薯，木薯，豌豆，芋头，香蕉，山药和谷类作物如水稻，玉米，小麦，大麦，燕麦和高粱。

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