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1. 发明申请

WO2002083720A3 INTERMEDIATES AND ENZYMES OF THE NON-MEVALONATE ISOPRENOID PATHWAY 审中-公开
公开(公告)号：WO2002083720A3
公开(公告)日：2002-10-24
申请号：PCT/EP2002/004005
申请日：2002-04-10
申请人： BACHER, Adelbert , ROHDICH, Felix , ADAM, Petra , AMSLINGER, Sabine , EISENREICH, Wolfgang , HECHT, Stefan
发明人： BACHER, Adelbert , ROHDICH, Felix , ADAM, Petra , AMSLINGER, Sabine , EISENREICH, Wolfgang , HECHT, Stefan
IPC分类号： C12N9/16
摘要： The invention provides a protein in a form that is functional for the enzymatic conversion of 2 C -methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its ( E )-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2butenyl 4-diphosphate, notably in its ( E )-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation.

2. 发明申请

WO2004065623A2 ASSAY FOR INHIBITORS OF ISPH 审中-公开
标题翻译：检测ISPH的抑制剂
公开(公告)号：WO2004065623A2
公开(公告)日：2004-08-05
申请号：PCT/EP2004/000264
申请日：2004-01-15
申请人： BACHER, Adelbert , ADAM, Petra , EISENREICH, Wolfgang , GRÄWERT, Tobias , HECHT, Stefan , ROHDICH, Felix , ZEPECK, Ferdinand
发明人： BACHER, Adelbert , ADAM, Petra , EISENREICH, Wolfgang , GRÄWERT, Tobias , HECHT, Stefan , ROHDICH, Felix , ZEPECK, Ferdinand
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26
摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) + ; c) repeating step (a) in the presence of the sample to be tested; d) repeating step (b) with the reaction mixture defined in step (c); e) comparing the results of steps (b) and (d).
摘要翻译：测定样品是否存在抑制1-羟基-2-甲基 - （E） - 丁烯基-4-二磷酸酯向异戊烯基二磷酸酯和/或二甲基烯丙基二磷酸酯的酶促转化的测定以下步骤：a）使含有1-羟基-2-甲基 - （E） - 丁烯基4-二磷酸酯，1-羟基-2-甲基 - （E） - 丁烯基-4-二磷酸还原酶和还原剂试剂在预定的反应条件下预定时间段; b）分析步骤（a）中获得的反应混合物中1-羟基-2-甲基 - （E） - 丁烯基4-二磷酸酯和/或NAD（P）H的消耗量和/或产生量的异戊烯基二磷酸盐和/或二甲基烯丙基二磷酸盐和/或NAD（P）+; c）在待测样品存在下重复步骤（a）; d）用步骤（c）中定义的反应混合物重复步骤（b）; e）比较步骤（b）和（d）的结果。

3. 发明申请

WO2004072276A1 CRYSTAL STRUCTURE OF 2-C-METHYL-D-ERYTHRITOL 4-PHOSPHATE SYNTHASE IN COMPLEX WITH INHIBITORS 审中-公开
标题翻译： 2-C-Methyl-D-ERYTHRITOL 4-PHOSPHATE SYNTHASE在复合物中的晶体结构与抑制剂
公开(公告)号：WO2004072276A1
公开(公告)日：2004-08-26
申请号：PCT/EP2004/001075
申请日：2004-02-05
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V. , BACHER, Adelbert , EISENREICH, Wolfgang , HECHT, Stefan , KAISER, Johannes , ROHDICH, Felix , HUBER, Robert , STEINBACHER, Stefan , GERHARDT, Stefan
发明人： BACHER, Adelbert , EISENREICH, Wolfgang , HECHT, Stefan , KAISER, Johannes , ROHDICH, Felix , HUBER, Robert , STEINBACHER, Stefan , GERHARDT, Stefan
IPC分类号： C12N9/04
CPC分类号： C12N9/0006 , C07K2299/00
摘要： The invention relates to a new crystal which comprises the protein 2C-methyl-D-erythritol 4-phosphate synthase (IspC). Further the crystal structure of IspC in complex with metal ions and a bound inhibitor is provided. Moreover, methods of identifying new inhibitors of IspC are described.
摘要翻译：本发明涉及包含蛋白质2C-甲基-D-赤藓糖醇-4-磷酸合成酶（IspC）的新晶体。此外，提供了与金属离子和结合抑制剂复合的IspC的晶体结构。此外，描述了鉴定IspC的新抑制剂的方法。

4. 发明申请

WO2009030654A1 MIKROBIOLOGISCHE HERSTELLUNG VON ISOPRENOIDEN 审中-公开
标题翻译：异戊二烯微生物生产
公开(公告)号：WO2009030654A1
公开(公告)日：2009-03-12
申请号：PCT/EP2008/061471
申请日：2008-09-01
申请人： EVONIK DEGUSSA GMBH , SIEBER, Volker , KAISER, Johannes , RÜHMANN, Broder , POTGRAVE, Nicole , WITTMANN, Eva-Maria , MARX, Achim , WINDHAB, Norbert , EWERING, Christian , KROLL, Jens , SCHULZE GRONOVER, Christian , PRÜFER, Professor Dirk , STEINBÜCHEL, Alexander , WURBS, David , BACHER, Adelbert , EISENREICH, Wolfgang , GRÄWERT, Tobias Wilhelm , ILLARINOVA, Victoria , GROLL, Michael , ROHDICH, Felix
发明人： SIEBER, Volker , KAISER, Johannes , RÜHMANN, Broder , POTGRAVE, Nicole , WITTMANN, Eva-Maria , MARX, Achim , WINDHAB, Norbert , EWERING, Christian , KROLL, Jens , SCHULZE GRONOVER, Christian , PRÜFER, Professor Dirk , STEINBÜCHEL, Alexander , WURBS, David , BACHER, Adelbert , EISENREICH, Wolfgang , GRÄWERT, Tobias Wilhelm , ILLARINOVA, Victoria , GROLL, Michael , ROHDICH, Felix
IPC分类号： C12N9/10 , C12P23/00 , C12R1/01 , C12R1/645
CPC分类号： C12P5/007 , C12N9/0006 , C12N9/1025 , C12N9/1085 , C12N9/1229 , C12N9/88 , C12N15/52
摘要： Die vorliegende Erfindung betrifft eine Zelle, welche gegenüber ihrem Wildtyp derart gentechnisch modifiziert wurde, dass sie in definierten Kompartimenten eingeschlossene Isoprenoide zu bilden vermag. Die Erfindung betrifft auch ein Verfahren zur Herstellung einer gentechnisch veränderten Zelle, die durch dieses Verfahren erhältliche, gentechnisch veränderte Zelle, ein Verfahren zur Herstellung von Isoprenoiden, die durch dieses Verfahren erhältlichen Isoprenoide, isolierte Nukleinsäuren sowie isolierte Polypeptide.
摘要翻译：本发明涉及已被以这样的方式相对于它的野生型使得它能够形成包封在类异戊二烯定义隔间遗传修饰的细胞。本发明还涉及一种方法，用于制造由本方法获得的遗传修饰的细胞，遗传修饰的细胞，用于生产类异戊二烯的方法，通过该方法获得的类异戊二烯，分离的核酸，以及分离的多肽。

5. 发明申请

WO2002092800A2 CRYSTAL STRUCTURE OF 2C-METHYL-D-ERYTHRITOL 2,4-CYCLODIPHOSPHATE SYNTHASE 审中-公开
标题翻译： 2-甲基-D-赖氨酸2,4-环己基磷酸酯合成酶的晶体结构
公开(公告)号：WO2002092800A2
公开(公告)日：2002-11-21
申请号：PCT/EP2002/005238
申请日：2002-05-13
申请人： MAX-PLANCK-GESELLSCHAFT , BACHER, Adelbert , HECHT, Stefan , HUBER, Robert , KAISER, Johannes , ROHDICH, Felix , STEINBACHER, Stefan
发明人： BACHER, Adelbert , HECHT, Stefan , HUBER, Robert , KAISER, Johannes , ROHDICH, Felix , STEINBACHER, Stefan
IPC分类号： C12N9/00
CPC分类号： C12Y406/01012 , C07K2299/00 , C12N9/88
摘要： This invention discloses the crystal structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase and crystal structures of said synthase with 4-diphosphocytidyl-2C-methyl-D-erythritol, 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate, cytidine monophosphate, cytidine diphosphate, cytidine and a combination of cytidine monophosphate and 2C-methyl-D-erythritol 2,4-cyclodiphosphate; with or without zinc. Further, computer-aided methods of identifying inhibitors of said synthase and inhibitors are provided.
摘要翻译：本发明公开了2-甲基-D-赤藓糖醇2,4-环二磷酸合成酶的晶体结构以及所述合成酶与4-二磷酸二巯基-2C-甲基-D-赤藓糖醇，4-二磷酸胞苷基-2C-甲基-D-赤藓糖醇的晶体结构二磷酸胞苷，胞苷二磷酸，胞苷和胞苷一磷酸和2-甲基-D-赤藓糖醇2,4-环二磷酸的组合; 有或没有锌。此外，提供了鉴定所述合成酶和抑制剂的抑制剂的计算机辅助方法。

6. 发明公开

EP1377663A2 INTERMEDIATES AND ENZYMES OF THE NON-MEVALONATE ISOPRENOID PATHWAY 审中-公开
标题翻译：异戊二烯独立的甲羟戊酸途径的中间体和酶
公开(公告)号：EP1377663A2
公开(公告)日：2004-01-07
申请号：EP02724284.1
申请日：2002-04-10
申请人： Bacher, Adelbert , Rohdich, Felix
发明人： BACHER, Adelbert , ROHDICH, Felix , ADAM, Petra , AMSLINGER, Sabine , EISENREICH, Wolfgang , HECHT, Stefan
IPC分类号： C12N9/16
CPC分类号： C07C33/025 , C07B2200/05 , C07F9/098 , C07F9/113 , C07H19/10 , C12N15/52 , C12P9/00 , C40B40/00 , G01N2500/04
摘要： The invention provides a protein in a form that is functional for the enzymatic conversion of 2C-methyl-D-erythritol 2,4-cyclodiphosphate to 1-hydroxy-2-methyl-2-butenyl 4-diphosphate notably in its (E)-form of the non-mevalonate biosynthetic pathway to isoprenoids. The invention also provides a protein in a form that is functional for the enzymatic conversion of 1-hydroxy-2-methyl-2butenyl 4-diphosphate, notably in its (E)-form, to isopentenyl diphosphate and/or dimethylallyl diphosphate. Further, screening methods for inhibitors of these proteins are provided. Further, 1-hydroxy-2-methyl-2-butenyl 4-diphosphate is provided and chemical and enzymatic methods of its preparation.

7. 发明申请

WO2012011103A2 METHODS AND COMPOSITIONS FOR INHIBITING THE NUCLEAR FACTOR kB PATHWAY 审中-公开
标题翻译：抑制核因子kB途径的方法和组合物
公开(公告)号：WO2012011103A2
公开(公告)日：2012-01-26
申请号：PCT/IL2011/000576
申请日：2011-07-19
申请人： BEN GURION UNIVERSITY OF THE NEGEV RESEARCH AND DEVELOPMENT AUTHORITY , MOR RESEARCH APPLICATIONS LTD. , GOLAN, Avi , GOPAS, Jacob , OZER, Janet , EISNER, Nadav , BACHER, Adelbert , EISENREICH, Wolfgang , OSTROZHENKOVA, Elena , WINER, Hila
发明人： GOLAN, Avi , GOPAS, Jacob , OZER, Janet , EISNER, Nadav , BACHER, Adelbert , EISENREICH, Wolfgang , OSTROZHENKOVA, Elena , WINER, Hila
IPC分类号： A61K31/4747
CPC分类号： A61K31/438 , A61K31/4747 , A61K31/7048 , A61K33/24 , A61K36/62 , A61K2300/00
摘要： The current invention provides therapeutic methods which include inhibition of nuclear factor κb pathway in a cell based on the discovery of an active fraction of a plant extract termed NUP or a composition which includes NUP. NUP is used in treating and managing different diseases such as cancer, inflammation, and virus infections.
摘要翻译：本发明提供了治疗方法，其包括基于发现名为NUP的植物提取物的活性部分或包含NUP的组合物来抑制细胞中的核因子κb途径。 NUP用于治疗和管理不同的疾病，如癌症，炎症和病毒感染。

8. 发明授权

EP1287145B1 THE NON-MEVALONATE ISOPRENOID PATHWAY 有权
标题翻译： THEMEVALONATUNABHÄNGIGE类异戊二烯
公开(公告)号：EP1287145B1
公开(公告)日：2008-11-26
申请号：EP01940547.1
申请日：2001-06-01
申请人： Bacher, Adelbert , Zenk, Meinhart H.
发明人： BACHER, Adelbert , ZENK, Meinhart, H. , ADAM, Petra , EISENREICH, Wolfgang , FELLERMEIER, Monika , HECHT, Stefan , ROHDICH, Felix , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
IPC分类号： C12N15/52 , C12N15/82 , C12N9/00 , C12P9/00 , A01H5/10
CPC分类号： C12N9/00 , C12N15/52 , C12N15/8274 , C12P9/00
摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.

9. 发明公开

EP1287145A2 THE NON-MEVALONATE ISOPRENOID PATHWAY 有权
标题翻译：非偏向性异丙肾上腺素途径
公开(公告)号：EP1287145A2
公开(公告)日：2003-03-05
申请号：EP01940547.1
申请日：2001-06-01
申请人： Bacher, Adelbert , Zenk, Meinhart H.
发明人： BACHER, Adelbert , ZENK, Meinhart, H. , ADAM, Petra , EISENREICH, Wolfgang , FELLERMEIER, Monika , HECHT, Stefan , ROHDICH, Felix , SCHUHR, Christoph, A. , WUNGSINTAWEEKUL, Juraithip
IPC分类号： C12N15/52 , C12N15/82 , C12N9/00 , C12P9/00 , A01H5/10
CPC分类号： C12N9/00 , C12N15/52 , C12N15/8274 , C12P9/00
摘要： The present invention is directed to enzymes and intermediates of the non-mevalonate isoprenoid pathway downstream of 2C-methyl-D-erythritol 2,4-cyclopyrophosphate and upstream of isopentenyl pyrophosphate or dimethylallyl pyrophosphate. These are used as a basis for a screening method for inhibitors of these enzymes, and a method for identifying inhibitor-resistant variants thereof. Further disclosures refer to DNA coding for said enzymes and for inhibitor-resistant variants thereof, vectors containing said DNA, cells containing said vector, and plant seeds comprising cells containing said vector. This invention is useful for the inhibition of the biosynthesis of isoprenoids in plants, bacteria and protozoa, for confering herbicide-resistance to plants, as well as for weed control in agriculture using a crop containing a herbicide-resistant gene and an effective amount of a suitable herbicide.
摘要翻译：本发明涉及2C甲基-D-赤藓糖醇2,4-环焦磷酸酯下游和异戊烯基焦磷酸或二甲基烯丙基焦磷酸上游的非甲羟戊酸类异戊二烯途径的酶和中间体。这些用作这些酶抑制剂筛选方法的基础，以及鉴定其抑制剂抗性变体的方法。其它公开内容涉及编码所述酶及其抑制剂抗性变体的DNA，含有所述DNA的载体，含有所述载体的细胞和含有含所述载体的细胞的植物种子。本发明可用于抑制植物，细菌和原生动物中类异戊二烯的生物合成，赋予植物除草剂抗性，以及在农业中用含有除草剂抗性基因和有效量的合适的除草剂。

10. 发明授权

EP1585826B1 ASSAY FOR INHIBITORS OF ISPH 有权
标题翻译：分析法的抑制剂ISPH
公开(公告)号：EP1585826B1
公开(公告)日：2006-11-22
申请号：EP04702324.7
申请日：2004-01-15
申请人： Bacher, Adelbert
发明人： BACHER, Adelbert , ADAM, Petra , EISENREICH, Wolfgang , GRÄWERT, Tobias , HECHT, Stefan , ROHDICH, Felix , ZEPECK, Ferdinand
IPC分类号： C12Q1/26
CPC分类号： C12Q1/26
摘要： Assay for testing a sample for the presence or absence of inhibition of the enzymatic conversion of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate into isopentenyl diphosphate and/or dimethylallyl diphosphate by the following steps: a) reacting an aqueous mixture containing 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate, a 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate reductase, and a reducing agent under predetermined reaction conditions for a predetermined period of time; b) analyzing the reaction mixture obtained in step (a) for the consumed amount of 1-hydroxy-2-methyl-(E)-butenyl 4-diphosphate and/or NAD(P)H and/or for the produced amount of isopentenyl diphosphate, and/or dimethylallyl diphosphate and/or NAD(P) ; c) repeating step (a) in the presence of the sample to be tested; d) repeating step (b) with the reaction mixture defined in step (c); e) comparing the results of steps (b) and (d).

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