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1. 发明授权

US08741560B2 Stable compositions for nucleic acid amplification and sequencing 失效
标题翻译：用于核酸扩增和测序的稳定组合物
公开(公告)号：US08741560B2
公开(公告)日：2014-06-03
申请号：US13345632
申请日：2012-01-06
申请人： Ayoub Rashtchian , Joseph Solus
发明人： Ayoub Rashtchian , Joseph Solus
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/02 , C07H21/04
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
摘要翻译：本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性较长时间。这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶各种医疗，法医和农业应用。特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

2. 发明申请

US20090092990A1 PRIMERS AND METHODS FOR THE DETECTION AND DISCRIMINATION OF NUCLEIC ACIDS 审中-公开
标题翻译：用于检测和鉴别核酸的原理和方法
公开(公告)号：US20090092990A1
公开(公告)日：2009-04-09
申请号：US12251378
申请日：2008-10-14
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

3. 发明申请

US20050042666A1 Primers and methods for the detection and discrimination of nucleic acids 审中-公开
标题翻译：用于检测和鉴别核酸的引物和方法
公开(公告)号：US20050042666A1
公开(公告)日：2005-02-24
申请号：US10932045
申请日：2004-09-02
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C07H21/04 , C12P19/34 , C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

4. 发明申请

US20100233699A1 PRIMERS AND METHODS FOR THE DETECTION AND DISCRIMINATION OF NUCLEIC ACIDS 有权
标题翻译：用于检测和鉴别核酸的原理和方法
公开(公告)号：US20100233699A1
公开(公告)日：2010-09-16
申请号：US12629045
申请日：2009-12-01
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard M. Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.
摘要翻译：本发明提供用于检测特异性核酸序列的新型引物和方法。本发明的引物和方法可用于多种分子生物学应用，并且特别适用于等位基因特异性PCR。

5. 发明授权

US08394609B2 Primers and methods for the detection and discrimination of nucleic acids 有权
公开(公告)号：US08394609B2
公开(公告)日：2013-03-12
申请号：US12629045
申请日：2009-12-01
申请人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
发明人： Irina Nazarenko , Ayoub Rashtchian , Joseph Solus , Richard Pires , Marlene Darfler , Gulilat Gebeyehu , Mekbib Astatke
IPC分类号： C12P19/34
CPC分类号： C12Q1/6876 , C12P19/34 , C12Q1/6816 , C12Q1/6827 , C12Q1/686 , C12Q1/6869 , G01N21/6486 , C12Q2525/121 , C12Q2525/161 , C12Q2563/107 , C12Q2565/107
摘要： The present invention provides novel primers and methods for the detection of specific nucleic acid sequences. The primers and methods of the invention are useful in a wide variety of molecular biology applications and are particularly useful in allele specific PCR.

6. 发明申请

US20120142007A1 STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 失效
标题翻译：用于核酸扩增和测序的稳定组合物
公开(公告)号：US20120142007A1
公开(公告)日：2012-06-07
申请号：US13345632
申请日：2012-01-06
申请人： Ayoub Rashtchian , Joseph Solus
发明人： Ayoub Rashtchian , Joseph Solus
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04 , C12N9/12
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
摘要翻译：本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性延长的时间。这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶各种医疗，法医和农业应用。特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

7. 发明申请

US20090233283A1 STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING 审中-公开
标题翻译：用于核酸扩增和测序的稳定组合物
公开(公告)号：US20090233283A1
公开(公告)日：2009-09-17
申请号：US12129597
申请日：2008-05-29
申请人： Ayoub Rashtchian , Joseph Solus
发明人： Ayoub Rashtchian , Joseph Solus
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
摘要翻译：本发明涉及包含试剂混合物的组合物，包括热稳定酶（例如热稳定的DNA聚合酶），缓冲液，辅因子和其它组分，适于立即用于核酸扩增或测序技术而不稀释或添加其它组分。组合物不含稳定剂（例如甘油或血清白蛋白），并且在高于冰点的温度下储存时，意外地保持活性较长时间。这些组合物单独或以试剂盒的形式用于核酸扩增（例如，通过聚合酶链式反应）和测序（例如，通过双脱氧或“Sanger”测序），或者对于使用热稳定性DNA聚合酶各种医疗，法医和农业应用。特别地，组合物和方法可用于扩增和测序尺寸大于约7千碱基的核酸分子。

8. 发明授权

US07501237B2 Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof 有权
标题翻译：用于分析或分型多态性核酸片段的聚合酶及其用途
公开(公告)号：US07501237B2
公开(公告)日：2009-03-10
申请号：US09891332
申请日：2001-06-27
申请人： Joseph Solus , Shuwei Yang , Deb K Chatterjee
发明人： Joseph Solus , Shuwei Yang , Deb K Chatterjee
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6858 , C12N9/1252 , Y10S435/81 , C12Q2525/151 , C12Q2521/101
摘要： The present invention provides methods for use in identifying, analyzing and typing polymorphic DNA fragments, particularly minisatellite, microsatellite or STR DNA fragments. In particular, the invention provides methods using DNA polymerases, more particularly thermostable DNA polymerases, and most particularly Thermotoga polymerases or mutants or derivatives thereof, whereby minisatellite, microsatellite or STR DNA molecules may be amplified and analyzed for polymorphisms. The invention also relates to polymerases having reduced, substantially reduced or eliminated ability to add non-template 3′ nucleotides to a synthesized nucleic acid molecule. In accordance with the invention, such reduction or elimination may be accomplished by modifying or mutating the desired polymerase.
摘要翻译：本发明提供了用于鉴定，分析和分型多形性DNA片段，特别是微型卫星，微卫星或STR DNA片段的方法。特别地，本发明提供了使用DNA聚合酶，更特别是耐热性DNA聚合酶，最特别是耐热性聚合酶或其突变体或衍生物的方法，由此可以扩增和分析微卫星，微卫星或STR DNA分子的多态性。本发明还涉及具有降低的，显着降低的或消除了向合成的核酸分子添加非模板3'核苷酸的能力的聚合酶。根据本发明，可以通过修饰或突变所需的聚合酶来实现这种还原或消除。

9. 发明授权

US06306588B1 Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof 失效
标题翻译：用于分析或分型多态性核酸片段的聚合酶及其用途
公开(公告)号：US06306588B1
公开(公告)日：2001-10-23
申请号：US09019160
申请日：1998-02-06
申请人： Joseph Solus , Shuwei Yang , Deb K. Chatterjee
发明人： Joseph Solus , Shuwei Yang , Deb K. Chatterjee
IPC分类号： C12Q168
CPC分类号： C12Q1/6858 , C12N9/1252 , Y10S435/81 , C12Q2525/151 , C12Q2521/101
摘要： The present invention provides methods for use in identifying, analyzing and typing polymorphic DNA fragments, particularly minisatellite, microsatellite or STR DNA fragments. In particular, the invention provides methods using DNA polymerases, more particularly thermostable DNA polymerases, and most particularly Thermotoga polymerases or mutants or derivatives thereof, whereby minisatellite, microsatellite or STR DNA molecules maybe amplified and analyzed for polymorphisms. The invention also relates to polymerases having reduced, substantially reduced or eliminated ability to add non-template 3′ nucleotides to a synthesized nucleic acid molecule. In accordance with the invention, such reduction or elimination may be accomplished by modifying or mutating the desired polymerase.
摘要翻译：本发明提供了用于鉴定，分析和分型多形性DNA片段，特别是微型卫星，微卫星或STR DNA片段的方法。特别地，本发明提供使用DNA聚合酶，更特别是耐热性DNA聚合酶的方法，最特别的是Thermotoga聚合酶或其突变体或衍生物，由此可以扩增和分析微卫星，微卫星或STR DNA分子的多态性。本发明还涉及具有降低的，显着降低的或消除了向合成的核酸分子添加非模板3'核苷酸的能力的聚合酶。根据本发明，可以通过修饰或突变所需的聚合酶来实现这种还原或消除。

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