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1. 发明授权

US08110394B2 Culture microscope apparatus 有权
标题翻译：培养显微镜装置
公开(公告)号：US08110394B2
公开(公告)日：2012-02-07
申请号：US12776182
申请日：2010-05-07
申请人： Kazuhiro Hasegawa , Kenichi Koyama , Atsuhiro Tsuchiya , Atsushi Yonetani
发明人： Kazuhiro Hasegawa , Kenichi Koyama , Atsuhiro Tsuchiya , Atsushi Yonetani
IPC分类号： C12M1/34 , G02B21/26
CPC分类号： C12M41/14 , C12M23/50 , C12M41/46 , G02B21/0088 , G02B21/06 , G02B21/24 , Y10S435/809
摘要： A culture microscope apparatus has an illumination unit to apply excitation light to the specimen, a specimen observing portion to observe light generated from the specimen due to the excitation light, and a dimmer unit to dim the excitation light that has penetrated the specimen.
摘要翻译：培养显微镜装置具有对试样施加激发光的照明单元，用于观察由于激发光而产生的试样的光的试样观察部，以及使透过试样的激发光变暗的调光单元。

2. 发明授权

US07816126B2 Culture observation apparatus, sample tray heat-insulating device and lid 有权
标题翻译：文化观察装置，样品托盘隔热装置和盖子
公开(公告)号：US07816126B2
公开(公告)日：2010-10-19
申请号：US11312114
申请日：2005-12-20
申请人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
发明人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
IPC分类号： C12M3/00 , C12M1/34
CPC分类号： C12M23/48 , C12M23/50 , C12M41/12
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and a culture medium; a microscope that serves for observing the cultured cell; a tray holding mechanism that holds the sample tray in the culture space in a detachable manner with good reproducibility; and a shifting mechanism that relatively shifts the sample tray held by the tray holding mechanism and a light axis of the microscope along a plane that is orthogonal to the light axis, wherein the sample tray has a heater used for heating the sample tray, and is electrically connectable to an energy supplying unit that supplies energy to the sample tray.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括形成培养空间的培养装置，该培养装置被控制为适于培养培养细胞的环境; 容纳容纳培养细胞的容器和培养基的样品盘; 用于观察培养细胞的显微镜; 托盘保持机构，以可拆卸的方式将样品盘保持在培养空间中，具有良好的再现性; 以及移动机构，其使由所述托盘保持机构保持的所述检体托盘和所述显微镜的光轴沿着与所述光轴正交的平面相对移动，所述检体托盘具有用于加热所述检体托盘的加热器，电连接到向样品托盘供应能量的能量供应单元。

3. 发明授权

US07595188B2 Observation apparatus 有权
标题翻译：观察装置
公开(公告)号：US07595188B2
公开(公告)日：2009-09-29
申请号：US11302591
申请日：2005-12-12
申请人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
发明人： Kazuhiro Hasegawa , Akitsugu Kagayama , Hideaki Endo , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Katsuyoshi Yamaguchi
IPC分类号： C12M3/00
CPC分类号： C12M23/50 , C12M23/10 , C12M23/48 , C12M41/36
摘要： An observation apparatus includes a sample tray that holds a container housing a cultured cell and a culture medium and covered with a lid; an observation unit that serves for observation of the cultured cell; and a shifting unit that relatively shifts the sample tray and a light axis of the observation unit along a plane that is orthogonal to the light axis of the observation unit, wherein the sample tray includes a container holding unit that holds the container by utilizing an elastic force, and at least one of the lid and one portion of the lid is made detachable, with the container being held in the sample tray by the container holding unit.
摘要翻译：观察装置包括：样本托盘，其容纳容纳培养细胞和培养基并容纳盖子的容器; 用于观察培养细胞的观察单元; 以及移动单元，其沿着与所述观察单元的光轴正交的平面相对地移动所述样本托盘和所述观察单元的光轴，其中，所述样本托盘包括容器保持单元，所述容器保持单元通过利用弹性并且盖子和盖子的一部分中的至少一个是可拆卸的，容器通过容器保持单元保持在样品盘中。

4. 发明授权

US07333265B2 Microscope apparatus 有权
标题翻译：显微镜装置
公开(公告)号：US07333265B2
公开(公告)日：2008-02-19
申请号：US11409695
申请日：2006-04-24
申请人： Kazuhiro Hasegawa , Atsuhiro Tsuchiya , Akitsugu Kagayama , Takashi Yoneyama , Katsuyoshi Yamaguchi
发明人： Kazuhiro Hasegawa , Atsuhiro Tsuchiya , Akitsugu Kagayama , Takashi Yoneyama , Katsuyoshi Yamaguchi
IPC分类号： G02B21/00
CPC分类号： G02B21/248 , G02B21/33
摘要： Provided is a microscope having an immersion objective lens, a nozzle, and a liquid supplying mechanism. The immersion objective lens condenses light from a sample through liquid. The nozzle supplies the liquid to an upper surface of the immersion objective lens. The liquid supplying mechanism cooperates with one of a lens moving mechanism that moves the immersion objective lens and a sample moving mechanism that maintains and moves the sample, and moves the nozzle relative to the immersion objective lens to supply the liquid.
摘要翻译：提供了具有浸没物镜，喷嘴和液体供给机构的显微镜。浸没式物镜通过液体将来自样品的光冷凝。喷嘴将液体供应到浸没物镜的上表面。液体供给机构与移动浸没物镜的透镜移动机构和保持并移动样品的样品移动机构相配合，并使喷嘴相对于浸没物镜移动以供给液体。

5. 发明申请

US20050105172A1 Culture microscope apparatus 有权
标题翻译：培养显微镜装置
公开(公告)号：US20050105172A1
公开(公告)日：2005-05-19
申请号：US10969464
申请日：2004-10-20
申请人： Kazuhiro Hasegawa , Kenichi Koyama , Atsuhiro Tsuchiya , Atsushi Yonetani
发明人： Kazuhiro Hasegawa , Kenichi Koyama , Atsuhiro Tsuchiya , Atsushi Yonetani
IPC分类号： C12M1/34 , G02B21/00 , G02B21/06 , G02B21/24
CPC分类号： C12M41/14 , C12M23/50 , C12M41/46 , G02B21/0088 , G02B21/06 , G02B21/24 , Y10S435/809
摘要： A culture microscope apparatus has an illumination unit to apply excitation light to the specimen, a specimen observing portion to observe light generated from the specimen due to the excitation light, and a dimmer unit to dim the excitation light that has penetrated the specimen.
摘要翻译：培养显微镜装置具有对试样施加激发光的照明单元，用于观察由于激发光而产生的试样的光的试样观察部，以及使透过试样的激发光变暗的调光单元。

6. 发明申请

US20070065936A1 Tissue culture microscope apparatus 有权
标题翻译：组织培养显微镜装置
公开(公告)号：US20070065936A1
公开(公告)日：2007-03-22
申请号：US11522730
申请日：2006-09-18
申请人： Kazuhiro Hasegawa , Akitsugu Kagayama , Atsuhiro Tsuchiya , Ryuichi Hirano , Kenichi Koyama , Hideaki Endo
发明人： Kazuhiro Hasegawa , Akitsugu Kagayama , Atsuhiro Tsuchiya , Ryuichi Hirano , Kenichi Koyama , Hideaki Endo
IPC分类号： C12M1/34 , G01N21/01
CPC分类号： G02B21/30 , C12M23/48 , C12M41/14 , C12M41/36 , C12M41/46 , G01N2035/00425 , Y10S435/809
摘要： A tissue culture microscope apparatus includes a culture unit that includes a chamber in which a specimen is put, and maintains the chamber at a predetermined temperature to culture the specimen; an observation unit that forms an observation image of the specimen put in the chamber; and a liquid supply unit that stores a liquid in a protrusion portion penetrating into a wall of the chamber and protruding to an inside of the chamber, matches a temperature of the liquid with the temperature of the chamber, and injects the liquid from the protrusion portion to the specimen.
摘要翻译：组织培养显微镜装置包括培养单元，该培养单元包括其中放置样本的室，并将室维持在预定温度以培养样品; 观察单元，其形成放置在所述室中的样本的观察图像; 以及液体供应单元，其将液体存储在突出部分中，所述突出部分穿入所述室的壁并突出到所述室的内部，使所述液体的温度与所述室的温度相匹配，并且将所述液体从所述突出部分标本。

7. 发明申请

US20060238885A1 Microscope apparatus 有权
标题翻译：显微镜装置
公开(公告)号：US20060238885A1
公开(公告)日：2006-10-26
申请号：US11409695
申请日：2006-04-24
申请人： Kazuhiro Hasegawa , Atsuhiro Tsuchiya , Akitsugu Kagayama , Takashi Yoneyama , Katsuyoshi Yamaguchi
发明人： Kazuhiro Hasegawa , Atsuhiro Tsuchiya , Akitsugu Kagayama , Takashi Yoneyama , Katsuyoshi Yamaguchi
IPC分类号： G02B21/02
CPC分类号： G02B21/248 , G02B21/33
摘要： Provided is a microscope having an immersion objective lens, a nozzle, and a liquid supplying mechanism. The immersion objective lens condenses light from a sample through liquid. The nozzle supplies the liquid to an upper surface of the immersion objective lens. The liquid supplying mechanism cooperates with one of a lens moving mechanism that moves the immersion objective lens and a sample moving mechanism that maintains and moves the sample, and moves the nozzle relative to the immersion objective lens to supply the liquid.
摘要翻译：提供了具有浸没物镜，喷嘴和液体供给机构的显微镜。浸没式物镜通过液体将来自样品的光冷凝。喷嘴将液体供应到浸没物镜的上表面。液体供给机构与移动浸没物镜的透镜移动机构和保持并移动样品的样品移动机构相配合，并使喷嘴相对于浸没物镜移动以供给液体。

8. 发明申请

US20060141609A1 Culture observation apparatus, sample tray heat-insulating device and lid 有权
标题翻译：文化观察装置，样品托盘隔热装置和盖子
公开(公告)号：US20060141609A1
公开(公告)日：2006-06-29
申请号：US11312114
申请日：2005-12-20
申请人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
发明人： Akitsugu Kagayama , Ryuichi Hirano , Atsuhiro Tsuchiya , Kenichi Koyama , Kazuhiro Hasegawa , Katsuyoshi Yamaguchi , Hideaki Endo
IPC分类号： C12M1/34
CPC分类号： C12M23/48 , C12M23/50 , C12M41/12
摘要： A culture observation apparatus, which is used for observing a cultured cell while culturing the cell, includes a culture device that forms a culture space which is controlled to be an environment suitable for culturing the cultured cell; a sample tray that holds a container housing the cultured cell and a culture medium; a microscope that serves for observing the cultured cell; a tray holding mechanism that holds the sample tray in the culture space in a detachable manner with good reproducibility; and a shifting mechanism that relatively shifts the sample tray held by the tray holding mechanism and a light axis of the microscope along a plane that is orthogonal to the light axis, wherein the sample tray has a heater used for heating the sample tray, and is electrically connectable to an energy supplying unit that supplies energy to the sample tray.
摘要翻译：用于在培养细胞时观察培养细胞的培养观察装置包括形成培养空间的培养装置，该培养装置被控制为适于培养培养细胞的环境; 容纳容纳培养细胞的容器和培养基的样品盘; 用于观察培养细胞的显微镜; 托盘保持机构，以可拆卸的方式将样品盘保持在培养空间中，具有良好的再现性; 以及移动机构，其使由所述托盘保持机构保持的所述检体托盘和所述显微镜的光轴沿着与所述光轴正交的平面相对移动，所述检体托盘具有用于加热所述检体托盘的加热器，电连接到向样品托盘供应能量的能量供应单元。

9. 发明授权

US07382531B2 Microscope and method of preventing dew condensation on objective lens 有权
标题翻译：显微镜及防止物镜结露的方法
公开(公告)号：US07382531B2
公开(公告)日：2008-06-03
申请号：US11259392
申请日：2005-10-26
申请人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
发明人： Atsuhiro Tsuchiya , Katsuyoshi Yamaguchi , Akitsugu Kagayama , Kazuhiro Hasegawa , Hideaki Endo , Kenichi Koyama
IPC分类号： G02B21/26
CPC分类号： G02B27/0006 , C12M41/12 , C12M41/36 , G02B7/008 , G02B21/30
摘要： The problem to be solved by the present invention is to provide a microscope that prevents a dew condensation on an objective lens and allows obtaining a good microscopic image, and a method of preventing a dew condensation on an objective lens.A microscope includes an objective lens having a heater 22 in a cell incubator 3 having a high humidity space. The high humidity space is maintained at 90% to 100% of relative humidity and controlled to be at a predetermined gas temperature. A cultured cell 12 in the cell incubator 3 is observed via the objective lens 1, and the heater 22 is controlled to make the temperature of the objective lens 1 higher than the gas temperature of the high humidity space.
摘要翻译：本发明要解决的问题是提供一种防止物镜上的结露并且可以获得良好的显微图像的显微镜，以及防止物镜上的结露的方法。显微镜包括在具有高湿度空间的细胞培养器3中具有加热器22的物镜。高湿度空间保持在相对湿度的90％至100％，并被控制在预定的气体温度。通过物镜1观察细胞培养器3中的培养细胞12，控制加热器22使物镜1的温度高于高湿度空间的气体温度。

10. 发明申请

US20070070498A1 Observation apparatus and observation method 有权
标题翻译：观察仪器和观察方法
公开(公告)号：US20070070498A1
公开(公告)日：2007-03-29
申请号：US11517874
申请日：2006-09-08
申请人： Hideaki Endo , Akitsugu Kagayama , Atsuhiro Tsuchiya , Kazuhiro Hasegawa
发明人： Hideaki Endo , Akitsugu Kagayama , Atsuhiro Tsuchiya , Kazuhiro Hasegawa
IPC分类号： G02B23/24
CPC分类号： G02B21/367
摘要： An observation apparatus includes an incident-light illumination optical system, a transmitting illumination optical system, an imaging unit, an input device, a storage unit, an imaging controller, a microscope controller, an imaging information management unit. The imaging information management unit compares an area position, of an imaging area of the imaging unit, indicated by position information input from the input device with an area position stored in the storage unit to determine whether the area position indicated by the position information is stored in the storage unit. When the area position is not stored in the storage unit, the imaging controller controls the imaging unit to image the specimen and to generate an observation image, and stores the observation image in the storage unit. The microscope controller controls one of the optical systems to illuminate the specimen only during an imaging period, when the imaging unit images the specimen.
摘要翻译：观察装置包括入射光照明光学系统，发射照明光学系统，成像单元，输入装置，存储单元，成像控制器，显微镜控制器，成像信息管理单元。成像信息管理单元将从输入设备输入的由位置信息指示的摄像单元的成像区域的区域位置与存储在存储单元中的区域位置进行比较，以确定是否存储由位置信息指示的区域位置在存储单元中。当区域位置不存储在存储单元中时，成像控制器控制成像单元对样本进行成像并生成观察图像，并将观察图像存储在存储单元中。显微镜控制器控制其中一个光学系统仅在成像周期期间，当成像单元对样本进行成像时照亮样本。

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