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1. 发明授权

US08278062B2 Method of using lipid acyltransferase 有权
标题翻译：脂质酰基转移酶的使用方法
公开(公告)号：US08278062B2
公开(公告)日：2012-10-02
申请号：US12400268
申请日：2009-03-09
申请人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
发明人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
IPC分类号： C12Q1/48 , C12N9/10
CPC分类号： A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses one or both of the following reactions: alcoholysis or transesterification.
摘要翻译：制备碳水化合物酯，蛋白质酯，蛋白质亚基酯或羟基酸酯中的一种或多种的方法，该方法包括将酰基供体，酰基受体和水混合以产生高水环境，其包含5-98％水，其中所述酰基供体是选自磷脂，溶血磷脂，三酰基甘油酯，甘油二酯，糖脂或溶血糖脂中的一种或多种的脂质底物，并且所述酰基受体选自一组或多于一种由碳水化合物，蛋白质，蛋白质亚基或羟基酸组成; 并将该混合物与脂质酰基转移酶接触，使得所述脂质酰基转移酶催化下列一个或两个反应：醇解或酯交换反应。

2. 发明授权

US07955813B2 Method of using lipid acyltransferase 有权
公开(公告)号：US07955813B2
公开(公告)日：2011-06-07
申请号：US11483345
申请日：2006-07-07
申请人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
发明人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
IPC分类号： C12Q1/48 , C12N9/10
CPC分类号： A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.

3. 发明授权

US08003095B2 Method of using lipid acyltransferase 有权
标题翻译：脂质酰基转移酶的使用方法
公开(公告)号：US08003095B2
公开(公告)日：2011-08-23
申请号：US11483331
申请日：2006-07-07
申请人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
发明人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
IPC分类号： A61K38/45 , C12Q1/48
CPC分类号： A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译：一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入食品中。优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。这些中的一种或多种可以用作另外的乳化剂。

4. 发明授权

US07955814B2 Method 有权
标题翻译：方法
公开(公告)号：US07955814B2
公开(公告)日：2011-06-07
申请号：US11671953
申请日：2007-02-06
申请人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe , Mark Turner , Jonathan Goodwins
发明人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe , Mark Turner , Jonathan Goodwins
IPC分类号： C12Q1/48 , C12N9/10
CPC分类号： C12Y301/01032 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23V2002/00 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an additional emulsifier.
摘要翻译：一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入食品中。优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。这些中的一种或多种可以用作另外的乳化剂。

5. 发明授权

US07807398B2 Method of using lipid acyltransferase 有权
标题翻译：脂质酰基转移酶的使用方法
公开(公告)号：US07807398B2
公开(公告)日：2010-10-05
申请号：US11182408
申请日：2005-07-15
申请人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
发明人： Arno De Kreij , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
IPC分类号： C12Q1/48 , C12N9/10
CPC分类号： A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method for the in situ production of an emulsifier in a foodstuff, wherein a lipid acyltransferase is added to the foodstuff. Preferably the emulsifier is produced without an increase or without a substantial increase in the free fatty acid content of the foodstuff. Preferably, the lipid acyltransferase is one which is capable of transferring an acyl group from a lipid to one or more of the following acyl acceptors: a sterol, a stanol, a carbohydrate, a protein or a sub-unit thereof, glycerol. Preferably, in addition to an emulsifier one or more of a stanol ester or a stanol ester or a protein ester or a carbohydrate ester or a diglyceride or a monoglyceride may be produced. One or more of these may function as an addition emulsifier.
摘要翻译：一种在食品中原位生产乳化剂的方法，其中将脂质酰基转移酶加入食品中。优选地，在不增加或不显着增加食品的游离脂肪酸含量的情况下生产乳化剂。优选地，脂质酰基转移酶是能够将酰基从脂质转移到以下酰基受体中的一种或多种：甾醇，甾烷醇，碳水化合物，蛋白质或其亚单位的甘油。优选地，除了乳化剂之外，可以制备一种或多种甾烷醇酯或甾烷醇酯或蛋白酯或碳水化合物酯或甘油二酯或甘油单酯。这些中的一种或多种可以用作加成乳化剂。

6. 发明申请

US20100285525A1 METHOD 有权
标题翻译：方法
公开(公告)号：US20100285525A1
公开(公告)日：2010-11-11
申请号：US12400268
申请日：2009-03-09
申请人： Arno DE KREIJ , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
发明人： Arno DE KREIJ , Susan Mampusti Madrid , Jørn Dalgaard Mikkelsen , Jørn Borch Søe
IPC分类号： C12P21/00 , C12P19/00 , C12P7/62
CPC分类号： A21D8/042 , A23C9/1216 , A23C19/0328 , A23C19/054 , A23D7/013 , A23G9/363 , A23L15/25 , A23L23/00 , A23L27/60 , A23L29/06 , A23L29/10 , A23L33/11 , A23V2002/00 , C11C3/10 , C12N9/1029 , C12P7/62 , C12P7/6454 , C12P7/6481 , C12Q1/48 , C12Y203/01 , C12Y301/01 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y301/01032 , G01N2333/91051 , G01N2333/918 , A23V2200/222 , A23V2250/192 , A23V2250/1846 , A23V2250/2136 , A23V2250/21372 , A23V2250/21362
摘要： A method of producing one or more of a carbohydrate ester, a protein ester, a protein subunit ester or a hydroxyl acid ester, which method comprises admixing an acyl donor, an acyl acceptor and water to produce a high water environment comprising 5-98% water, wherein said acyl donor is a lipid substrate selected from one or more of the group consisting of a phospholipid, a lysophospholipid, a triacylglyceride, a diglyceride, a glycolipid or a lysoglycolipid and said acyl acceptor is selected from one ore more of the group consisting of a carbohydrate, a protein, a protein subunit, or a hydroxyl acid; and contacting the admixture with a lipid acyltransferase, such that said lipid acyl transferase catalyses one or both of the following reactions: alcoholysis or transesterification.
摘要翻译：制备碳水化合物酯，蛋白质酯，蛋白质亚基酯或羟基酸酯中的一种或多种的方法，该方法包括将酰基供体，酰基受体和水混合以产生高水环境，其包含5-98％水，其中所述酰基供体是选自磷脂，溶血磷脂，三酰基甘油酯，甘油二酯，糖脂或溶血糖脂中的一种或多种的脂质底物，并且所述酰基受体选自一组或多于一种由碳水化合物，蛋白质，蛋白质亚基或羟基酸组成; 并将该混合物与脂质酰基转移酶接触，使得所述脂质酰基转移酶催化下列一个或两个反应：醇解或酯交换反应。

7. 发明授权

US09493748B2 Method for treating pyropheophytin-containing compositions 有权
标题翻译：用于处理含有嗜铬素素的组合物的方法
公开(公告)号：US09493748B2
公开(公告)日：2016-11-15
申请号：US13321869
申请日：2010-06-10
申请人： Jørn Borch Søe , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi , Jens Frisbaæk Sørensen , Tina Jørgensen , Janne Brunstedt , Rene Mikkelsen , Susan Mampusti Madrid
发明人： Jørn Borch Søe , Charlotte Horsmans Poulsen , Masoud Rajabi Zargahi , Jens Frisbaæk Sørensen , Tina Jørgensen , Janne Brunstedt , Rene Mikkelsen , Susan Mampusti Madrid
IPC分类号： C12P7/64 , A23D9/04 , C11B3/00 , C12N9/18
CPC分类号： C12N9/18 , A23L5/49 , C11B3/003
摘要： The present invention provides methods and uses for treating pyropheophytin-containing compositions, particularly in order to remove pyropheophytin therefrom. The compositions are typically plant, algal, or bacterial derived products such as vegetable oils. The method comprises a step of contacting the compositions with an enzyme having pyropheophytinase activity. Also provided are related apparatus and products for performing such methods and uses.
摘要翻译：本发明提供了治疗含有含有嗜铬细胞的组合物的方法和用途，特别是为了从其中除去焦皮苷。组合物通常是植物，藻类或细菌衍生的产品，例如植物油。该方法包括使组合物与具有嗜铬素酶活性的酶接触的步骤。还提供了用于执行这些方法和用途的相关设备和产品。

8. 发明授权

US06406723B1 Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase 有权
标题翻译：使用甘油氧化酶和脂肪酶制备由这种面团制成的面粉面团和产品的方法
公开(公告)号：US06406723B1
公开(公告)日：2002-06-18
申请号：US09402664
申请日：1999-10-22
申请人： Jørn Borch Søe , Charlotte Horsmans Poulsen , Preben Rasmussen , Susan Mampusti Madrid , Masoud R. Zargahi
发明人： Jørn Borch Søe , Charlotte Horsmans Poulsen , Preben Rasmussen , Susan Mampusti Madrid , Masoud R. Zargahi
IPC分类号： A21D804
CPC分类号： C12Y101/03005 , A21D2/16 , A21D2/26 , A21D8/042 , A23L7/107 , C07K2319/00 , C12N9/20 , C12N9/80 , C12Y301/01003 , C12Y301/01004 , C12Y301/01026 , C12Y302/00
摘要： A method of improving the rheological properties of a flour dough and the quality of bread, alimentary paste products, noodles and cakes produced therefrom, wherein a combination of (a) a glycerol oxidase which does not require a co-factor to oxidize glycerol, and (b) a lipase, is added to the dough to produce a synergistic effect upon said rheological properties; and dough improving compositions containing such components.
摘要翻译：一种改善面粉生面团的流变性质和由其生产的面包，消化糊产品，面条和蛋糕质量的方法，其中（a）不需要辅助因子来氧化甘油的甘油氧化酶的组合，以及（b）脂肪酶加入到面团中以对所述流变性质产生协同效应; 和含有这些组分的生面团改进组合物。

9. 发明申请

US20140051146A1 METHOD 审中-公开
标题翻译：方法
公开(公告)号：US20140051146A1
公开(公告)日：2014-02-20
申请号：US14000933
申请日：2012-02-16
申请人： Susan Mampusti Madrid
发明人： Susan Mampusti Madrid
IPC分类号： C12N9/18
CPC分类号： C12N9/18 , C11B3/003 , C12N15/80 , C12Y301/01014
摘要： In one aspect there is provided a method for producing a recombinant enzyme capable of hydrolysing chlorophyll or a chlorophyll derivative, comprising a step of intracellular expression of the recombinant enzyme in a eukaryotic host cell.
摘要翻译：一方面，提供了能够水解叶绿素或叶绿素衍生物的重组酶的方法，其包括在真核宿主细胞中细胞内表达重组酶的步骤。

10. 发明授权

US08017341B2 Transcription factors 有权
标题翻译：转录因子
公开(公告)号：US08017341B2
公开(公告)日：2011-09-13
申请号：US11738355
申请日：2007-04-20
申请人： Igor Nikolaev , Susan Mampusti Madrid
发明人： Igor Nikolaev , Susan Mampusti Madrid
IPC分类号： C12Q1/68 , C12N15/00 , C12N1/15 , C07H21/04
CPC分类号： C07K14/38 , C12N15/80 , C12N2501/60
摘要： The present invention relates to an isolated nucleic acid sequence coding for a PntR transcription factor comprising a nucleotide sequence that is the same as, or is complementary to, or contains any suitable codon substitutions for any of those of SEQ ID NOs: 1, 3 or 5 or comprises a sequence which has at least 60% sequence homology with any of SEQ ID NOs: 1, 3 or 5, as well as corresponding polypeptides, vector systems and host cells comprising the same. In particular, the invention relates to sequences which are obtained from an Aspergillus, Trichoderma or Penicillium cell. The invention further relates to methods for disrupting PntR expression in a cell as well as methods for expression or production of a POI in a host cell in which PntR expression has been disrupted.
摘要翻译：本发明涉及编码PntR转录因子的分离的核酸序列，所述核酸序列包含与SEQ ID NO：1,3或任何SEQ ID NO：1,3中任一个相同或互补的任何合适的密码子取代的核苷酸序列或或包含与SEQ ID NO：1,3或5中任一个具有至少60％序列同源性的序列，以及相应的多肽，载体系统和包含该序列的宿主细胞。特别地，本发明涉及从曲霉属，木霉属或青霉属细胞获得的序列。本发明还涉及破坏细胞中PntR表达的方法以及在PntR表达被破坏的宿主细胞中表达或产生POI的方法。

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