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    • 9. 发明申请
    • Nucleic Acid Amplification and Sequencing on a Droplet Actuator
    • 滴液致动器中的核酸扩增和测序
    • US20110311980A1
    • 2011-12-22
    • US13139467
    • 2009-12-15
    • Michael G. PollackAllen E. EckhardtVijay SrinivasanPrasanna Thwar
    • Michael G. PollackAllen E. EckhardtVijay SrinivasanPrasanna Thwar
    • C12Q1/68C12M1/34
    • B01L3/502761B01L3/502784B01L3/502792B01L7/525B01L2200/10B01L2200/143B01L2300/0816B01L2300/0838B01L2400/0427B01L2400/043B01L2400/0644C12Q1/6869C12Q2565/301C12Q2565/626
    • The invention provides a droplet actuator device, as well as systems, methods and devices making use of the droplet actuator device. The droplet actuator device may include a substrate having electrodes arranged for conducting one or more droplet operations. The droplet actuator device may include a substrate having a reactor path with a wash region associated with a magnet for immobilizing mobilizing beads during bead washing operations. The droplet actuator device may include nucleotide base reservoirs and dedicated nucleotide base electrode paths arranged for transporting nucleotide base droplets from nucleotide base reservoirs to the reactor path. The droplet actuator device may include one or more wash buffer reservoirs associated with electrode paths arranged for transporting wash buffer droplets from wash buffer reservoirs to the reactor path. The droplet actuator device may include one or more sample reservoirs and sample paths arranged for transporting sample droplets from the one or more sample reservoirs to the reactor path. The droplet actuator device may include one or more enzyme reservoirs and dedicated enzyme electrode paths arranged for transporting enzyme droplets from the one or more enzyme reservoirs to a detection electrode.
    • 本发明提供一种液滴致动器装置,以及利用液滴致动器装置的系统,方法和装置。 液滴致动器装置可以包括具有布置用于进行一个或多个液滴操作的电极的基板。 液滴致动器装置可以包括具有反应器路径的基底,其具有与磁珠相关联的洗涤区域,用于在珠洗涤操作期间固定动员珠粒。 液滴致动器装置可以包括核苷酸碱基储存器和专用核苷酸碱基电极路径,其设置用于将核苷酸碱基液滴从核苷酸碱基储存器输送到反应器路径。 液滴致动器装置可以包括一个或多个洗涤缓冲液储存器,其与设置用于​​将洗涤缓冲液滴从洗涤缓冲液储存器运送到反应器路径的电极路径相关联。 液滴致动器装置可以包括一个或多个样品储存器和样品路径,其被布置用于将样品液滴从一个或多个样品储存器输送到反应器路径。 液滴致动器装置可以包括一个或多个酶储器和用于将酶液滴从一个或多个酶储存器输送到检测电极的专用酶电极路径。