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1. 发明专利

JP2008048621A Method for creating chimeric adenovirus and pharmaceutical using the same 审中-公开
标题翻译：使用该方法创造重型腺病毒和药物的方法
公开(公告)号：JP2008048621A
公开(公告)日：2008-03-06
申请号：JP2006225392
申请日：2006-08-22
申请人： Aristo:Kk , Chiba Prefecture , 千葉県 , 株式会社アリスト
发明人： TAGAWA MASATOSHI , KAWAMURA KIYOKO , TAKENOBU NAONORI
IPC分类号： C12N7/00 , A61K35/14 , A61K35/28 , A61K35/76 , A61P35/00 , C12N15/09
CPC分类号： C12N15/86 , A61K35/761 , C12N2710/10332 , C12N2710/10343 , C12N2810/60 , C12N2810/6018 , C12N2830/008
摘要： PROBLEM TO BE SOLVED: To provide a new chimeric adenovirus vector, to provide a method for efficiently creating the same, and to provide pharmaceuticals to which the new chimeric adenovirus vector is applied. SOLUTION: The new chimeric adenovirus is created from a vector DNA with the fiber knob region of type 35 adenovirus integrated in type 5 adenovirus and a second vector DNA where the expression of E1A and E1B genes of the type 5 adenovirus is made controllable by an extraneous transcriptional control region. This chimeric adenovirus, which a modified type 5 adenovirus, is such that its fiber knob region is substituted by the fiber knob region of the type 35 adenovirus and the E1A transcriptional control region is eliminated, and at the resulting site, an arbitrary extraneous transcriptional control region controlling the expression of the E1A and E1B genes is introduced. This chimeric adenovirus is capable of melting cells or tumors, thus being applicable, for example, to pharmaceuticals having high cell-damaging activity against intractable tumors. COPYRIGHT: (C)2008,JPO&INPIT
摘要翻译：待解决的问题：提供一种新的嵌合腺病毒载体，以提供有效产生该嵌合腺病毒载体的方法，并提供应用新的嵌合腺病毒载体的药物。解决方案：新型嵌合腺病毒由载体DNA产生，其中腺病毒5型腺病毒的纤维旋钮区域集成在5型腺病毒中，第2种载体DNA使5型腺病毒E1A和E1B基因的表达可控制通过外来的转录控制区。这种嵌合腺病毒是修饰型5型腺病毒，使得其纤维旋钮区域被35型腺病毒的纤维旋钮区域和E1A转录控制区域取代，并且在所得位点处，任意的外来转录控制介绍了控制E1A和E1B基因表达的区域。该嵌合腺病毒能够融化细胞或肿瘤，因此可用于例如对难治性肿瘤具有高细胞破坏活性的药物。版权所有（C）2008，JPO＆INPIT

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