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1. 发明申请

US20100183617A1 COMPOSITIONS AND METHODS FOR REGULATING SAS1R 审中-公开
标题翻译：用于调节SAS1R的组合物和方法
公开(公告)号：US20100183617A1
公开(公告)日：2010-07-22
申请号：US12613947
申请日：2009-11-06
申请人： John C. Herr , Monika Sachdev , Arabinda Mandal
发明人： John C. Herr , Monika Sachdev , Arabinda Mandal
IPC分类号： A61K39/395 , G01N33/567 , C07K16/18 , A61K39/00 , A61K38/46
CPC分类号： C07K16/28 , A61K38/1709 , A61K38/177 , A61K38/4886 , A61K39/0006 , C07K2317/76 , G01N2333/96486 , G01N2500/04
摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.
摘要翻译：本发明提供了用于调节受精和用作基于本发明的卵母细胞特异性蛋白质SAS1R（精子顶体SLLP1受体）的组合物和方法，其是精子蛋白受体。确定了六种SAS1R变体，包括全长SAS1R。 mSLLP1和SAS1R共同定位于卵母细胞和顶体反应精子的顶体。通过远西分析，在严格选择条件下的酵母双杂交系统中，通过抗mSLLP1和相反的免疫沉淀法证明了mSLLP1和SAS1R之间的相互作用。发现纯化的重组SAS1R具有蛋白酶活性，在体外抑制受精，并在雌性中诱导免疫应答。结果表明，SAS1R是精子顶体内配体SLLP1的蛋白水解活性，卵母细胞和早期胚胎特异性卵母细胞金属蛋白酶受体，是调节受精和避孕的目标。

2. 发明申请

US20090214552A1 Novel Egg Receptors for Sperm Proteins 审中-公开
标题翻译：用于精子蛋白的新型蛋受体
公开(公告)号：US20090214552A1
公开(公告)日：2009-08-27
申请号：US11915225
申请日：2006-02-21
申请人： Arabinda Mandal , Monika Sachdev , John C. Herr
发明人： Arabinda Mandal , Monika Sachdev , John C. Herr
IPC分类号： A61K39/395 , A61K31/711 , A61K38/17 , A61P15/18
CPC分类号： C12N9/6421 , C07K14/705
摘要： The present invention relates to novel egg membrane protein receptors and to methods of inhibiting the interaction of sperm proteins with egg proteins. The invention further relates to methods of preventing and inhibiting sperm-egg binding, sperm-egg fusion, and fertilization. The invention further relates to the egg membrane proteins MET and ZEP.
摘要翻译：本发明涉及新型卵膜蛋白受体以及抑制精蛋白与卵蛋白相互作用的方法。本发明还涉及预防和抑制精子卵结合，精卵融合和受精的方法。本发明还涉及蛋膜蛋白质MET和ZEP。

3. 发明授权

US09803012B2 Compositions and methods for regulating SAS1R 有权
公开(公告)号：US09803012B2
公开(公告)日：2017-10-31
申请号：US13479167
申请日：2012-05-23
申请人： John C. Herr , Monika Sachdev , Arabinda Mandal
发明人： John C. Herr , Monika Sachdev , Arabinda Mandal
IPC分类号： C07K16/28 , A61K38/17 , A61K38/48 , A61K39/00
CPC分类号： C07K16/28 , A61K38/1709 , A61K38/177 , A61K38/4886 , A61K39/0006 , C07K2317/76 , G01N2333/96486 , G01N2500/04
摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.

4. 发明申请

US20120252031A1 COMPOSITIONS AND METHODS FOR REGULATING SAS1R 有权
标题翻译：用于调节SAS1R的组合物和方法
公开(公告)号：US20120252031A1
公开(公告)日：2012-10-04
申请号：US13479167
申请日：2012-05-23
申请人： John C. Herr , Monika Sachdev , Arabinda Mandal
发明人： John C. Herr , Monika Sachdev , Arabinda Mandal
IPC分类号： C12Q1/37 , G01N33/573
CPC分类号： C07K16/28 , A61K38/1709 , A61K38/177 , A61K38/4886 , A61K39/0006 , C07K2317/76 , G01N2333/96486 , G01N2500/04
摘要： The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.
摘要翻译：本发明提供了用于调节受精和用作基于本发明的卵母细胞特异性蛋白质SAS1R（精子顶体SLLP1受体）的组合物和方法，其是精子蛋白受体。确定了六种SAS1R变体，包括全长SAS1R。 mSLLP1和SAS1R共同定位于卵母细胞和顶体反应精子的顶体。通过远西分析，在严格选择条件下的酵母双杂交系统中，通过抗mSLLP1和相反的免疫沉淀法证明了mSLLP1和SAS1R之间的相互作用。发现纯化的重组SAS1R具有蛋白酶活性，在体外抑制受精，并在雌性中诱导免疫应答。结果表明，SAS1R是精子顶体内配体SLLP1的蛋白水解活性，卵母细胞和早期胚胎特异性卵母细胞金属蛋白酶受体，是调节受精和避孕的目标。

5. 发明授权

US07947026B2 Glans compatible single unit semen collection and storage device, kit and related methods of use 有权
标题翻译： Glans兼容单位精液收集和储存装置，试剂盒及相关使用方法
公开(公告)号：US07947026B2
公开(公告)日：2011-05-24
申请号：US10577983
申请日：2004-11-05
申请人： John C. Herr , Arabinda Mandal
发明人： John C. Herr , Arabinda Mandal
IPC分类号： A61F5/44
CPC分类号： A61B10/0058 , A61F6/04
摘要： A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices.
摘要翻译：一种符合龟头阴茎的方法和装置，以完全恢复精液中的精液，以防止初始精子丰富的附睾部分的损失，避免使用避孕套进行手淫，以消除射精后多次精液转运与目前的方法相同，并且提供包含适合于龟头阴茎的精液收集室的单个装置。该装置在无菌包装中的一个整体形成的模块中提供储存和测量容器，可选的帽和垂直支架。该装置和方法将在多种情况下具有多种用途，包括但不限于：在不育症的诊断中，精液捐献，体外受精（IVF）/细胞质内精子注射（ICSI）诊所，医院，法医学实验室和研究实验室，并将被纳入用于非处方精子测试装置的试剂盒中。

6. 发明申请

US20080318250A1 Compositions and Methods for Identifying Sperm for Forensic Applications 审中-公开
标题翻译：识别精子用于法医应用的组合物和方法
公开(公告)号：US20080318250A1
公开(公告)日：2008-12-25
申请号：US10588445
申请日：2005-02-07
申请人： Linda Gilmer , Arabinda Mandal , Michael J. Wolkowicz , Kenneth L. Klotz , John C. Herr
发明人： Linda Gilmer , Arabinda Mandal , Michael J. Wolkowicz , Kenneth L. Klotz , John C. Herr
IPC分类号： G01N33/53 , C07H1/00 , C12P19/34
CPC分类号： C07K16/28 , G01N33/56966
摘要： Methods and compositions for identifying and isolating sperm cells from samples containing multiple cell types are described. The methods and compositions employ antibodies that specifically bind to sperm-specific antigens located on or internal to the sperm plasma membrane. A reporter molecule may be conjugated to the antibodies to aid in the detection of sperm. The antibodies may be targeted to sperm-specific antigens in the head and/or tail of sperm to facilitate the identification and isolation of sperm cells from forensic samples prepared from sexual assault evidence. Purified DNA from the isolated sperm cells can be amplified by polymerase chain reaction to assist forensic analysis in sexual assault cases.
摘要翻译：描述了从含有多种细胞类型的样品中鉴定和分离精子细胞的方法和组合物。方法和组合物使用特异性结合位于精子质膜上或内部的精子特异性抗原的抗体。报告分子可以与抗体缀合以帮助检测精子。抗体可以针对精子头部和/或尾部的精子特异性抗原，以促进从性攻击证据准备的法医样品中鉴定和分离精子细胞。可以通过聚合酶链反应扩增分离的精子细胞的纯化DNA，以协助性攻击病例的法医分析。

7. 发明授权

US07125550B2 Human sperm specific lysozyme-like proteins 失效
公开(公告)号：US07125550B2
公开(公告)日：2006-10-24
申请号：US10181611
申请日：2001-01-19
申请人： John C. Herr , Friederike Jayes , Arabinda Mandal , Jagathpala Shetty , Michael J. Wolkowicz
发明人： John C. Herr , Friederike Jayes , Arabinda Mandal , Jagathpala Shetty , Michael J. Wolkowicz
IPC分类号： A61K39/395 , A61K39/40 , A61K39/42 , C12N5/00 , C12N5/02
CPC分类号： C12N9/2462 , A61K39/0006 , C07K16/40 , C12Y302/01017 , G01N2500/00 , Y10S514/841
摘要： The present invention relates to two novel, testis-specific proteins (C19 and C23) that arc lysozyme paralogues. The proteins are believed to play a role in capacitation of sperm and the fertilization of the ovum. Therefore these compounds make ideal targets for the design of contraceptive agents. The C19 and C23 proteins can also be modified to establish lysozyme activity and the modified proteins can then be used in all applications that currently exist for lysozymes.

8. 发明申请

US20070031895A1 Glans compatible single unit semen collection and storage device, kit and related methods of use 有权
标题翻译： Glans兼容单位精液收集和储存装置，试剂盒及相关使用方法
公开(公告)号：US20070031895A1
公开(公告)日：2007-02-08
申请号：US10577983
申请日：2004-11-05
申请人： John Herr , Arabinda Mandal
发明人： John Herr , Arabinda Mandal
IPC分类号： G01N33/567 , A61M31/00
CPC分类号： A61B10/0058 , A61F6/04
摘要： A method and device to conform with the glans penis to recover ejaculated semen completely, to prevent the loss of initial sperm rich epididymal fractions, to avoid the use of a condom for masturbation, to eliminate the multi-step transfers of semen following ejaculation that are common with current methods, and to provide a single device that contains a semen collecting chamber that fits onto a glans penis. The device provides a storage and measuring reservoir, an optional cap, and a vertical stand in one integrally formed module in a sterile pack. The device and method will have multiple uses in a variety of contexts including, but not limited thereto: in the diagnosis of infertility, in semen donation, in vitro fertilization (IVF)/intra-cytoplasmic sperm injection (ICSI) clinics, hospitals, forensic laboratories and research laboratories and will be included in kits intended for over-the-counter sperm testing devices
摘要翻译：一种符合龟头阴茎的方法和装置，以完全恢复精液中的精液，以防止初始精子丰富的附睾部分的损失，避免使用避孕套进行手淫，以消除射精后多次精液转运与目前的方法相同，并且提供包含适合于龟头阴茎的精液收集室的单个装置。该装置在无菌包装中的一个整体形成的模块中提供储存和测量容器，可选的帽和垂直支架。该装置和方法将在多种情况下具有多种用途，包括但不限于：在不育症的诊断中，精液捐献，体外受精（IVF）/细胞质内精子注射（ICSI）诊所，医院，法医学实验室和研究实验室，并将被纳入用于非处方精子测试装置的试剂盒中

9. 发明申请

US20060089297A1 Sperm specific lysozyme-like proteins 审中-公开
标题翻译：精子特异性溶菌酶样蛋白
公开(公告)号：US20060089297A1
公开(公告)日：2006-04-27
申请号：US10542038
申请日：2004-01-16
申请人： John Herr , Maria Herrero , Arabinda Mandal , Laura Digilio
发明人： John Herr , Maria Herrero , Arabinda Mandal , Laura Digilio
IPC分类号： A61K38/17 , C07K14/47
CPC分类号： C07K14/705 , A61K38/1709 , A61K38/47 , A61K49/0002 , C07K16/40 , C12N9/2462 , G01N33/689 , G01N2500/02 , G01N2800/367
摘要： The present invention is directed to a family of testis specific proteins (SLLPs) that share high sequence identity to lysozyme-C proteins. The application encompasses compositions comprising the SLLP proteins, antibodies specific for the SLLP polypeptides and the use of the SLLP polypeptides and antibodies directed to such peptides as contraceptive agents.
摘要翻译：本发明涉及与溶菌酶-C蛋白具有高序列同一性的睾丸特异性蛋白（SLLP）家族。该应用包括包含SLLP蛋白质，对SLLP多肽特异的抗体以及SLLP多肽的使用以及针对这些肽作为避孕剂的抗体的组合物。

10. 发明申请

US20060062799A1 Sperm specific lysozyme-like proteins 审中-公开
标题翻译：精子特异性溶菌酶样蛋白
公开(公告)号：US20060062799A1
公开(公告)日：2006-03-23
申请号：US11269323
申请日：2005-11-08
申请人： John Herr , Friederike Jayes , Arabinda Mandal , Jagathpala Shetty , Michael Wolkowicz
发明人： John Herr , Friederike Jayes , Arabinda Mandal , Jagathpala Shetty , Michael Wolkowicz
IPC分类号： A61K39/00 , C12N9/36 , C07H21/04 , C12P21/06
CPC分类号： C12Y302/01017 , C12N9/2462
摘要： The present invention relates to two novel, testis-specific proteins (C19 and C23) that are lysozyme paralogues. The proteins are believed to play a role in capacitation of sperm and the fertilization of the ovum. Therefore these compounds make ideal targets for the design of contraceptive agents. The C19 and C23 proteins can also be modified to establish lysozyme activity and the modified proteins can then be used in all applications that currently exist for lysozymes.
摘要翻译：本发明涉及两种新型的睾丸特异性蛋白（C19和C23），它们是溶菌酶旁系同源物。据信这些蛋白质在精子获能和卵子受精中起作用。因此，这些化合物是设计避孕药物的理想目标。也可以修饰C19和C23蛋白以建立溶菌酶活性，然后将修饰的蛋白质用于目前存在于溶菌酶的所有应用中。

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