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1. 发明申请

US20120015347A1 Methods for Assaying Cellular Binding Interactions 有权
标题翻译：分析细胞结合相互作用的方法
公开(公告)号：US20120015347A1
公开(公告)日：2012-01-19
申请号：US13184363
申请日：2011-07-15
申请人： Anupam Singhal , Carl L. G. Hansen , John W. Schrader , Charles A. Haynes , Daniel J. Da Costa
发明人： Anupam Singhal , Carl L. G. Hansen , John W. Schrader , Charles A. Haynes , Daniel J. Da Costa
IPC分类号： C12Q1/70 , C12M1/34 , C12Q1/68 , G01N33/566 , G01N33/577
CPC分类号： G01N33/56966 , B01L3/502738 , B01L3/502761 , B01L2200/0668 , B01L2300/0681 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0481 , G01N33/577 , G01N33/582 , G01N33/6854
摘要： There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
摘要翻译：提供了用于测定由细胞产生的蛋白质（例如单克隆抗体）和生物分子之间的结合相互作用的方法和装置。该方法可以包括将细胞保持在具有孔的腔室内; 将由细胞产生的蛋白质暴露于捕获底物，其中所述捕获底物与由所述细胞产生的蛋白质流体连通，并且其中所述捕获底物可操作地结合由所述细胞产生的蛋白质; 使包含所述生物分子的流体体积经由所述孔流过所述腔室，其中所述流体体积与所述捕获衬底流体连通; 并确定由细胞产生的蛋白质与生物分子之间的结合相互作用。

2. 发明授权

US09188593B2 Methods for assaying cellular binding interactions 有权
标题翻译：测定细胞结合相互作用的方法
公开(公告)号：US09188593B2
公开(公告)日：2015-11-17
申请号：US13184363
申请日：2011-07-15
申请人： Anupam Singhal , Carl L. G. Hansen , John W. Schrader , Charles A. Haynes , Daniel J. Da Costa
发明人： Anupam Singhal , Carl L. G. Hansen , John W. Schrader , Charles A. Haynes , Daniel J. Da Costa
IPC分类号： B01L3/00 , G01N33/68 , G01N33/569
CPC分类号： G01N33/56966 , B01L3/502738 , B01L3/502761 , B01L2200/0668 , B01L2300/0681 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2400/0481 , G01N33/577 , G01N33/582 , G01N33/6854
摘要： There are provided methods, and devices for assaying for a binding interaction between a protein, such as a monoclonal antibody, produced by a cell, and a biomolecule. The method may include retaining the cell within a chamber having an aperture; exposing the protein produced by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein produced by the cell and wherein the capture substrate is operable to bind the protein produced by the cell; flowing a fluid volume comprising the biomolecule through the chamber via said aperture, wherein the fluid volume is in fluid communication with the capture substrate; and determining a binding interaction between the protein produced by the cell and the biomolecule.
摘要翻译：提供了用于测定由细胞产生的蛋白质（例如单克隆抗体）和生物分子之间的结合相互作用的方法和装置。该方法可以包括将细胞保持在具有孔的腔室内; 将由细胞产生的蛋白质暴露于捕获底物，其中所述捕获底物与由所述细胞产生的蛋白质流体连通，并且其中所述捕获底物可操作地结合由所述细胞产生的蛋白质; 使包含所述生物分子的流体体积经由所述孔流过所述腔室，其中所述流体体积与所述捕获衬底流体连通; 并确定由细胞产生的蛋白质与生物分子之间的结合相互作用。

3. 发明授权

US5627052A Methods for the production of proteins with a desired function 失效
标题翻译：生产具有所需功能的蛋白质的方法
公开(公告)号：US5627052A
公开(公告)日：1997-05-06
申请号：US464261
申请日：1995-06-05
申请人： John W. Schrader
发明人： John W. Schrader
IPC分类号： C07K16/00 , C07K16/24 , C07K16/28 , A61K39/395
CPC分类号： C07K16/00 , C07K16/246 , C07K16/28
摘要： The present invention provides a method for producing proteins with a desired function, generally comprising the steps of (a) providing a population of antibody-forming cells suspected of containing at least one cell capable of producing an antibody exhibiting a desired function; (b) suspending the population of antibody-forming cells in a medium, the medium having an indicator system incorporated therein, the indicator system also being capable of indicating the presence and location of a cell which forms antibodies exhibiting the desired function; (c) identifying a cell forming an antibody exhibiting the desired function; (d) isolating the identified antibody-forming cell from the medium; (e) determining the amino acid sequence of the variable region or a portion thereof which coffers the desired function of the antibody produced by the isolated antibody-forming cell; and (f) synthesizing a protein with a desired function, the protein containing the amino acid sequence of the variable region or portion thereof which confers the desired function.
摘要翻译：本发明提供了一种产生具有所需功能的蛋白质的方法，通常包括以下步骤：（a）提供怀疑含有至少一种能够产生具有所需功能的抗体的细胞的抗体形成细胞群; （b）将抗体形成细胞群悬浮在培养基中，其中包含指示剂系统的培养基，指示剂系统还能够指示形成具有所需功能的抗体的细胞的存在和位置; （c）鉴定形成具有所需功能的抗体的细胞; （d）从培养基中分离所鉴定的抗体形成细胞; （e）确定可分区或其部分的氨基酸序列，其中所述可变区或其部分涵盖由分离的抗体形成细胞产生的抗体的所需功能; 和（f）合成具有所需功能的蛋白质，所述蛋白质含有赋予所需功能的可变区或其部分的氨基酸序列。

4. 发明申请

US20170327565A1 CROSS-PROTECTIVE PATHOGEN PROTECTION, METHODS AND COMPOSITIONS THEREOF 审中-公开
公开(公告)号：US20170327565A1
公开(公告)日：2017-11-16
申请号：US15616226
申请日：2017-06-07
申请人： John W. Schrader
发明人： John W. Schrader
IPC分类号： C07K16/10 , A61K39/145 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/545 , A61K2039/575 , C07K16/1018 , C07K2317/21 , C07K2317/33 , C07K2317/565 , C07K2317/622 , C07K2317/76 , C12N2760/16134
摘要： The present disclosure provides a method of inducing a cross-protective immune response in a subject against a pathogen, such as influenza, comprising administering a first unique pathogen antigen to the subject; and administering a second unique pathogen antigen 3-52 weeks after a); wherein the second unique pathogen antigen and the first unique pathogen antigen are immunologically distinct but share conserved sites that are not normally immunogenic for antibodies. Also disclosed herein are assays for detecting cross-protective antibodies, methods of generating novel cross-protective antibodies. Further provided are novel antibodies against influenza.

5. 发明授权

US10463728B2 Cross-protective pathogen protection, methods and compositions thereof 有权
公开(公告)号：US10463728B2
公开(公告)日：2019-11-05
申请号：US15616226
申请日：2017-06-07
申请人： John W. Schrader
发明人： John W. Schrader
IPC分类号： C07K16/10 , G01N33/569 , C12N7/00 , C07K14/005 , A61K39/145 , A61K39/12 , A61K39/00
摘要： The present disclosure provides a method of inducing a cross-protective immune response in a subject against a pathogen, such as influenza, comprising administering a first unique pathogen antigen to the subject; and administering a second unique pathogen antigen 3-52 weeks after a); wherein the second unique pathogen antigen and the first unique pathogen antigen are immunologically distinct but share conserved sites that are not normally immunogenic for antibodies. Also disclosed herein are assays for detecting cross-protective antibodies, methods of generating novel cross-protective antibodies. Further provided are novel antibodies against influenza.

6. 发明授权

US09707288B2 Cross-protective pathogen protection, methods and compositions thereof 有权
公开(公告)号：US09707288B2
公开(公告)日：2017-07-18
申请号：US13811467
申请日：2011-07-22
申请人： John W. Schrader
发明人： John W. Schrader
IPC分类号： A61K39/145 , C07K16/10 , C07K14/005 , G01N33/569 , C12Q1/70 , A61K39/12 , A61K39/00
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/545 , A61K2039/575 , C07K16/1018 , C07K2317/21 , C07K2317/33 , C07K2317/565 , C07K2317/622 , C07K2317/76 , C12N2760/16134
摘要： The present disclosure provides a method of inducing a cross-protective immune response in a subject against a pathogen, such as influenza, comprising administering a first unique pathogen antigen to the subject; and administering a second unique pathogen antigen 3-52 weeks after a); wherein the second unique pathogen antigen and the first unique pathogen antigen are immunologically distinct but share conserved sites that are not normally immunogenic for antibodies. Also disclosed herein are assays for detecting cross-protective antibodies, methods of generating novel cross-protective antibodies. Further provided are novel antibodies against influenza.

7. 发明申请

US20130129747A1 CROSS-PROTECTIVE PATHOGEN PROTECTION, METHODS AND COMPOSITIONS THEREOF 审中-公开
标题翻译：交叉保护病原体保护，其方法和组合物
公开(公告)号：US20130129747A1
公开(公告)日：2013-05-23
申请号：US13811467
申请日：2011-07-22
申请人： John W. Schrader
发明人： John W. Schrader
IPC分类号： A61K39/145 , A61K39/42
CPC分类号： A61K39/145 , A61K39/12 , A61K2039/5252 , A61K2039/545 , A61K2039/575 , C07K16/1018 , C07K2317/21 , C07K2317/33 , C07K2317/565 , C07K2317/622 , C07K2317/76 , C12N2760/16134
摘要： The present disclosure provides a method of inducing a cross-protective immune response in a subject against a pathogen, such as influenza, comprising administering a first unique pathogen antigen to the subject; and administering a second unique pathogen antigen 3-52 weeks after a); wherein the second unique pathogen antigen and the first unique pathogen antigen are immunologically distinct but share conserved sites that are not normally immunogenic for antibodies. Also disclosed herein are assays for detecting cross-protective antibodies, methods of generating novel cross-protective antibodies. Further provided are novel antibodies against influenza.
摘要翻译：本公开提供了在受试者中针对病原体（例如流感）诱导交叉保护性免疫应答的方法，包括向受试者施用第一独特的病原体抗原; 和a）之后3-52周施用第二种独特的病原体抗原; 其中所述第二独特的病原体抗原和所述第一独特的病原体抗原是免疫学上不同的，但共享对于抗体通常不具有免疫原性的保守位点。本文还公开了用于检测交叉保护性抗体的测定法，产生新型交叉保护性抗体的方法。进一步提供了抗流感的新型抗体。

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