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1. 发明申请

US20070264702A1 Method of Regulating Heterologous Protein Expression Controlled by Salicylic Deratives in Micro-Organisms Associated with Higher Organisms 审中-公开
标题翻译：调节与高等生物相关的微生物中水杨酸衍生物控制的异源蛋白表达的方法
公开(公告)号：US20070264702A1
公开(公告)日：2007-11-15
申请号：US10581025
申请日：2004-11-26
申请人： Angel Cebolla Ramirez , Jose Luis Sanchez-Palencia , Eduardo Santurino
发明人： Angel Cebolla Ramirez , Jose Luis Sanchez-Palencia , Eduardo Santurino
IPC分类号： C12N1/36
CPC分类号： C12N15/635 , A61K2039/522 , A61K2039/523
摘要： The invention relates to a method which can be used to control the expression of heterologous proteins in micro-organisms using an expression system controlled by salicylate or derivatives. The genetic system can be used in attenuated pathogenic bacteria that can be induced once hosted by the eukaryotic cell by concentrations within the pharmacology safety range. The tropism of some bacteria for certain tissues or organs can be used to control the in-situ production of biomolecules for research purposes and as a system for the controlled release of biopharmaceuticals, e.g. in order to control the expression of anti-tumour proteins or antigens.
摘要翻译：本发明涉及一种可用于使用由水杨酸盐或衍生物控制的表达系统来控制微生物中异源蛋白质表达的方法。遗传系统可用于减毒病原菌，一旦由真核细胞宿主，药物安全范围内的浓度就可以诱导。用于某些组织或器官的某些细菌的向性可用于控制用于研究目的的生物分子的原位生成，以及用于控制释放生物药物的系统，例如，以控制抗肿瘤蛋白或抗原的表达。

2. 发明申请

US20080280355A1 Control of Gene Expression with the Use of a Transcription Attenuator 审中-公开
标题翻译：使用转录衰减物控制基因表达
公开(公告)号：US20080280355A1
公开(公告)日：2008-11-13
申请号：US11667007
申请日：2005-10-11
申请人： Eduardo Santero Santurino , Angel Cebolla Ramirez , Jose Luis Royo Sanchez-Palencia
发明人： Eduardo Santero Santurino , Angel Cebolla Ramirez , Jose Luis Royo Sanchez-Palencia
IPC分类号： C12N15/74
CPC分类号： C12N15/70 , C12N15/63 , C12N15/74
摘要： This invention relates to a system for the expression of heterologous genes, comprising an attenuator element which inhibits the elongation of the transcription of the heterologous genes, the expression of which is to be controlled, and two regulating modules which control the expression of the attenuator element. The invention also relates to the use of said expression system for the amplification of the expression of recombinant proteins, RNAs or apolipoproteins in bacteria. The invention further relates to vectors containing said expression system.
摘要翻译：本发明涉及用于表达异源基因的系统，其包括抑制异源基因的转录延伸的衰减子元件，其表达被控制，以及控制衰减子元件表达的两个调控模块。本发明还涉及所述表达系统在细菌中扩增重组蛋白质，RNA或载脂蛋白的表达的用途。本发明还涉及含有所述表达系统的载体。

3. 发明授权

US08329877B2 Methods for separating recombinant proteins in aqueous two-phase systems 失效
标题翻译：在两相体系中分离重组蛋白的方法
公开(公告)号：US08329877B2
公开(公告)日：2012-12-11
申请号：US12723356
申请日：2010-03-12
申请人： Jesús M. Sanz Morales , Beatriz Maestro Garcia-Donas , Miguel Arevalo Rodriguez , Isabel Velasco Umpierrez , Angel Cebolla Ramirez
发明人： Jesús M. Sanz Morales , Beatriz Maestro Garcia-Donas , Miguel Arevalo Rodriguez , Isabel Velasco Umpierrez , Angel Cebolla Ramirez
IPC分类号： A23J1/00 , C07K1/00 , C07K14/00 , C07K16/00 , C07K17/00
CPC分类号： C07K1/145
摘要： The object of the present invention is a procedure for the distribution, separation and purification in aqueous solution of recombinant proteins, based on the utilization of polypeptides with choline affinity. The invention is based on a phenomenon consisting of that two aqueous solutions with determinated components can be mixed, being distributed finally in two phases with different density. The fusionated proteins to said polypeptides with choline affinity are preferably located in one of the phases, while most of the cell extract proteins tend to go to the opposite phase. After a series of washings for removing the rest of the not desired material, this location can be inverted through the addition of a soluble molecule with affinity by the polypeptide fusionated to the protein of interest. This procedure allows modulating at convenience the presence of the protein or polypeptide of interest in one phase or another, possibiliting its purification with a high yield and purity grade. The invention represents an economic and scalable way of recombinant protein separation labelled preferably with choline-binding domains.
摘要翻译：本发明的目的是基于利用胆碱亲合力的多肽来分配，分离和纯化重组蛋白质的水溶液的方法。本发明是基于这样的现象，其中可以混合具有确定组分的两种水溶液，最终以不同的密度两相分配。具有胆碱亲和力的所述多肽的融合蛋白优选位于一个相中，而大多数细胞提取物蛋白倾向于进入相反阶段。在一系列用于除去其余不需要的材料的洗涤液之后，通过与融合到感兴趣的蛋白质的多肽的亲和力加入可溶性分子，可以反转该位置。该方法允许在一个阶段或另一个阶段方便地调节感兴趣的蛋白质或多肽的存在，可能以高产率和纯度等级进行纯化。本发明代表优选用胆碱结合结构域标记的重组蛋白分离的经济和可扩展的方法。

4. 发明申请

US20100234567A1 METHODS FOR SEPARATING RECOMBINANT PROTEINS IN AQUEOUS TWO-PHASE SYSTEMS 失效
标题翻译：在两相系统中分离重组蛋白的方法
公开(公告)号：US20100234567A1
公开(公告)日：2010-09-16
申请号：US12723356
申请日：2010-03-12
申请人： Jesús M. SANZ MORALES , Beatriz Maestro Garcia-Donas , Miguel Arevalo Rodriguez , Isabel Velasco Umpierrez , Angel Cebolla Ramirez
发明人： Jesús M. SANZ MORALES , Beatriz Maestro Garcia-Donas , Miguel Arevalo Rodriguez , Isabel Velasco Umpierrez , Angel Cebolla Ramirez
IPC分类号： C07K1/14
CPC分类号： C07K1/145
摘要： The object of the present invention is a procedure for the distribution, separation and purification in aqueous solution of recombinant proteins, based on the utilization of polypeptides with choline affinity. The invention is based on a phenomenon consisting of that two aqueous solutions with determinated components can be mixed, being distributed finally in two phases with different density. The fusionated proteins to said polypeptides with choline affinity are preferably located in one of the phases, while most of the cell extract proteins tend to go to the opposite phase. After a series of washings for removing the rest of the not desired material, this location can be inverted through the addition of a soluble molecule with affinity by the polypeptide fusionated to the protein of interest. This procedure allows modulating at convenience the presence of the protein or polypeptide of interest in one phase or another, possibiliting its purification with a high yield and purity grade. The invention represents an economic and scalable way of recombinant protein separation labelled preferably with choline-binding domains.
摘要翻译：本发明的目的是基于利用胆碱亲合力的多肽来分配，分离和纯化重组蛋白质的水溶液的方法。本发明是基于这样的现象，其中可以混合具有确定组分的两种水溶液，最终以不同的密度两相分配。具有胆碱亲和力的所述多肽的融合蛋白优选位于一个相中，而大多数细胞提取物蛋白倾向于进入相反阶段。在一系列用于除去其余不需要的材料的洗涤液之后，通过与融合到感兴趣的蛋白质的多肽的亲和力加入可溶性分子，可以反转该位置。该方法允许在一个阶段或另一个阶段方便地调节感兴趣的蛋白质或多肽的存在，可能以高产率和纯度等级进行纯化。本发明代表优选用胆碱结合结构域标记的重组蛋白分离的经济和可扩展的方法。

5. 发明申请

US20090215154A1 Method of preparing micro-and nanometric particles with labile Products 审中-公开
标题翻译：用不稳定产品制备微米和纳米颗粒的方法
公开(公告)号：US20090215154A1
公开(公告)日：2009-08-27
申请号：US11918699
申请日：2006-05-03
申请人： Miguel Alfonso Ganan Calvo , Sebastien Chavez De Diego , Angel Cebolla Ramirez , Maria Flores Mosquera , Elena De Castro Hernandez
发明人： Miguel Alfonso Ganan Calvo , Sebastien Chavez De Diego , Angel Cebolla Ramirez , Maria Flores Mosquera , Elena De Castro Hernandez
IPC分类号： C12N1/20 , B29B9/00 , C07K2/00 , C07H21/00 , C07H21/02 , C07H21/04 , C12N7/00
CPC分类号： A61K9/5138 , A23L29/256 , A23L29/288 , A23P10/30 , A23P10/40 , A61K9/5161 , A61K9/5192 , B01F13/0062 , B01J13/04 , B82Y30/00
摘要： The invention relates to a method of obtaining micro- and nanometric polymeric particles in a controlled, reproducible manner. The aforementioned particles have a spherical shape and a very narrow, uniform size distribution. The invention comprises the use of an easy particle-forming method consisting in using hydrodynamic forces to focus a composite microjet formed by two concentric fluids and can be used in the encapsulation of fragile compounds of biological interest, from peptides and proteins to cells and micro-organisms.
摘要翻译：本发明涉及以受控的，可再生的方式获得微米和纳米聚合物颗粒的方法。上述颗粒具有球形和非常窄且均匀的尺寸分布。本发明包括使用容易的颗粒形成方法，其包括使用流体动力来聚焦由两个同心流体形成的复合微喷射，并且可以用于将生物学上易受害的脆弱化合物从肽和蛋白质包封到细胞和微生物，生物体。

6. 发明授权

US06803224B2 Regulated expression of cloned genes using a cascade genetic circuit 失效
标题翻译：使用级联遗传电路调节克隆基因的表达
公开(公告)号：US06803224B2
公开(公告)日：2004-10-12
申请号：US09746567
申请日：2000-12-21
申请人： Angel Cebolla Ramirez , Carolina Sousa Martin , Victor de Lorenzo Prieto
发明人： Angel Cebolla Ramirez , Carolina Sousa Martin , Victor de Lorenzo Prieto
IPC分类号： C12N121
CPC分类号： C12N15/70 , C12N15/63
摘要： The present invention relates to the design, preparation and use of cascade regulatory circuits for amplification of gene expression. The genetic circuit is based on a plurality (e.g., two or more) of regulatory genes organized in a hierarchical order of expression in a genetic construct or constructs, which can be established in a cell, e.g., a gram-negative bacteria, by means of autoreplicative vectors or by chromosomal insertion. In one embodiment, the genetic construct(s) can be stabily maintained in the chromosome without selective pressure, and gene expression induced three orders of magnitude therefrom using economical biodegradable benzoate derivatives.
摘要翻译：本发明涉及用于扩增基因表达的级联调节电路的设计，制备和应用。基因回路基于以遗传构建体或构建体中的等级顺序组织的多个（例如两个或更多个）调节基因，其可以通过手段在细胞例如革兰氏阴性细菌中建立的自身复制载体或通过染色体插入。在一个实施方案中，遗传构建体可以在没有选择压力的情况下稳定地保持在染色体中，并且使用经济的可生物降解的苯甲酸酯衍生物从基因表达诱导三个数量级。

7. 发明授权

US09410962B2 Determination of levels of immunogenic gluten peptides in human samples 有权
标题翻译：人体样品中免疫原性麸质肽水平的测定
公开(公告)号：US09410962B2
公开(公告)日：2016-08-09
申请号：US13977247
申请日：2011-12-28
申请人： Carolina Sousa Martin , Isabel Comino Montilla , Ana Real Calderon , Santiago Vivas Alegre , Angel Cebolla Ramirez
发明人： Carolina Sousa Martin , Isabel Comino Montilla , Ana Real Calderon , Santiago Vivas Alegre , Angel Cebolla Ramirez
IPC分类号： G01N33/24 , G01N33/68 , G01N33/531 , G01N33/53
CPC分类号： G01N33/68 , G01N33/5308 , G01N2333/415 , G01N2800/065
摘要： The present invention, fitted in the medical-clinical sector, shows a method for monitoring the ingestion of gluten by measuring protein/gluten peptides present in fecal samples with antibodies against immunogenic peptides resistant to gastrointestinal digestion. The presence or absence of said immunogenic peptides is controlled by immunological assays based on reactive antibodies against immunogenic gluten peptides that are resistant to proteolysis. These assays may be quantitative techniques as ELISAs, or qualitative as rapid immunochromatographic assays, immunoblots, etc. These measures may also be applied to verify compliance with the gluten-free diet, to improve diagnosis in cases of refractory or severe symptoms of celiac disease, in cases in which a gluten-free diet is supposedly being respected, or to clinical research on the effectiveness of enzymatic therapies related with prolamin detoxification.
摘要翻译：安装在医疗临床领域的本发明显示了一种通过测量粪便样品中存在的蛋白质/谷蛋白肽来监测麸质摄入的方法，该抗体针对抗胃肠消化的免疫原性肽。所述免疫原性肽的存在或不存在通过基于对抗蛋白水解作用的免疫原性麸质肽的反应性抗体的免疫测定来控制。这些测定可以是作为ELISA的定量技术，或定性为快速免疫色谱测定，免疫印迹等。这些测量还可以用于验证对无麸质饮食的依从性，以改善在难治或严重的乳糜泻症状的情况下的诊断，在无麸质饮食被认为是受尊重的情况下，或临床研究有关醇溶素解毒的酶疗法的有效性。

8. 发明授权

US08283125B2 Peptide and protein preservation method 失效
标题翻译：肽和蛋白质保存方法
公开(公告)号：US08283125B2
公开(公告)日：2012-10-09
申请号：US12682690
申请日：2008-09-05
申请人： Angel Cebolla Ramirez , Moisés Alvarez Maqueda
发明人： Angel Cebolla Ramirez , Moisés Alvarez Maqueda
IPC分类号： G01N33/53
CPC分类号： C07K1/22
摘要： The invention relates to a method for preserving peptides or proteins over long periods of time. The invention also relates to the use of the preservation method for the transport of labeled peptides or proteins without requiring refrigeration, as well as to a set of tools for implementing said method.
摘要翻译：本发明涉及长时间保存肽或蛋白质的方法。本发明还涉及保存方法用于运输标记的肽或蛋白质而不需要制冷的用途，以及用于实施所述方法的一组工具。

9. 发明申请

US20100256301A1 PEPTIDE AND PROTEIN PRESERVATION METHOD 失效
标题翻译：肽和蛋白质保存方法
公开(公告)号：US20100256301A1
公开(公告)日：2010-10-07
申请号：US12682690
申请日：2008-09-05
申请人： Angel Cebolla Ramirez , Moisés Alvarez Maqueda
发明人： Angel Cebolla Ramirez , Moisés Alvarez Maqueda
IPC分类号： C07K1/04 , C08G63/91 , C07K16/00 , C08F12/00
CPC分类号： C07K1/22
摘要： The invention relates to a method for preserving peptides or proteins over long periods of time. The invention also relates to the use of the preservation method for the transport of labeled peptides or proteins without requiring refrigeration, as well as to a set of tools for implementing said method.
摘要翻译：本发明涉及长时间保存肽或蛋白质的方法。本发明还涉及保存方法用于运输标记的肽或蛋白质而不需要制冷的用途，以及用于实施所述方法的一组工具。

10. 发明申请

US20090214655A1 Method and Device for Obtaining Micro and Nanometric Size Particles 审中-公开
标题翻译：用于获得微米和纳米尺寸颗粒的方法和装置
公开(公告)号：US20090214655A1
公开(公告)日：2009-08-27
申请号：US11794509
申请日：2006-01-26
申请人： Alfonso Miguel Ganan Calvo , Lucia Martin Banderas , Maria Flores Mosquera , Alfonso Rodriguez Gil , Sebastian Chavez De Diego , Angel Cebolla Ramirez
发明人： Alfonso Miguel Ganan Calvo , Lucia Martin Banderas , Maria Flores Mosquera , Alfonso Rodriguez Gil , Sebastian Chavez De Diego , Angel Cebolla Ramirez
IPC分类号： A61K9/14 , B29B9/00 , G01N33/00 , C40B40/00
CPC分类号： G01N33/531 , B01J13/04 , B05B7/06 , B05B7/061 , G01N33/533 , G01N33/54346 , Y10T436/10
摘要： The invention relates to a method and device for obtaining micro and nanometric particles in a controlled, reproducible manner. The aforementioned particles have a spherical shape and a very narrow, uniform size distribution. More specifically, the invention relates to a novel method of forming emulsions and to the application thereof in micro and nanoencapsulation techniques involving the extraction/evaporation of the solvent. In particular, the invention relates to the encapsulation of the fluorescent compounds and the subsequent application thereof.
摘要翻译：本发明涉及以受控的，可再现的方式获得微米和纳米颗粒的方法和装置。上述颗粒具有球形和非常窄且均匀的尺寸分布。更具体地说，本发明涉及形成乳液的新方法及其在涉及萃取/蒸发溶剂的微纳和微囊技术中的应用。特别地，本发明涉及荧光化合物的包封及其后续应用。

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