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1. 发明授权

US5935442A Liquid fluid bed chromatography using conglomerates of controlled density 失效
标题翻译：液体流化床色谱法使用密度可控的密集体
公开(公告)号：US5935442A
公开(公告)日：1999-08-10
申请号：US971860
申请日：1993-03-08
申请人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g-Hansen
发明人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g-Hansen
IPC分类号： B01D39/14 , B01D15/02 , B01D15/18 , B01J2/08 , B01J2/16 , B01J8/00 , B01J8/08 , B01J8/18 , B01J8/20 , B01J8/22 , B01J8/24 , B01J8/32 , B01J13/00 , B01J13/02 , B01J20/22 , B01J20/28 , B01J20/281 , B01J20/283 , B01J20/284 , B01J20/285 , B01J20/32 , B01J47/10 , C02F1/28 , C02F1/72 , C02F3/10 , C02F3/12 , C02F3/34 , C07B63/00 , C08K3/00 , C08K3/22 , C08K5/00 , C08L101/00 , C12M1/40 , G01N30/58 , G01N30/88 , B01D15/08 , B01D15/00 , G01N30/02
CPC分类号： B01J20/28019 , B01D15/1807 , B01J13/02 , B01J2/16 , B01J20/28004 , B01J20/28011 , B01J20/28014 , B01J20/28021 , B01J20/28026 , B01J20/2803 , B01J20/3219 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J20/3274 , B01J47/10 , B01J8/008 , B01J8/085 , B01J8/1845 , B01J8/1872 , B01J8/1881 , B01J8/20 , B01J8/22 , B01J8/32 , C02F1/286 , C02F1/722 , C02F3/085 , C02F3/10 , C02F3/108 , C02F3/342 , B01D2215/021 , Y02W10/15 , Y10S435/815 , Y10S530/811 , Y10S530/812 , Y10S530/813
摘要： A fluidized bed chromatographic process for the purification and binding of molecules in a liquid to an active substance covalently bound to chromatographic adsorbent particles. The adsorbent particles are formed of a porous composite material having pores allowing access to the interior thereof and consisting of at least two density controlling particles of low or high density, or both, and a matrix formed by consolidating at least one conglomerating agent. The density controlling particles are dispersed in the matrix. The adsorbent particles have a relative density with respect to the liquid which is less than 0.95 and greater than 1.1, and they have a particle size within the range of 50-750 .mu.m. The relative density and particle size range of the adsorbent particles are selected to provide desired floatation/sedimentation properties of the adsorbent particles in the liquid in the fluidized bed process.
摘要翻译： PCT No.PCT / DK91 / 00195 Sec。 371日期1993年3月8日 102（e）1993年3月8日PCT PCT 1991年7月8日PCT公布。第WO92 / 00799号公报日期1992年1月23日用于将液体中的分子与共价结合到色谱吸附剂颗粒的活性物质进行纯化和结合的流化床色谱法。吸附剂颗粒由多孔复合材料形成，该多孔复合材料具有允许进入其内部并由至少两个低密度或高密度的密度控制颗粒或两者的孔组成的孔，以及通过固结至少一个聚集剂形成的基体。密度控制颗粒分散在基质中。吸附剂颗粒相对于液体的相对密度小于0.95且大于1.1，并且它们的粒度在50-750μm的范围内。选择吸附剂颗粒的相对密度和颗粒尺寸范围以在流化床工艺中提供液体中吸附剂颗粒的期望的浮选/沉降特性。

2. 发明授权

US6043067A Distributing liquid in a fluid bed reactor into turbulent and non-turbulent zones 失效
标题翻译：将流体床反应器中的液体分配到湍流和非湍流区域
公开(公告)号：US6043067A
公开(公告)日：2000-03-28
申请号：US003830
申请日：1998-01-08
申请人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g-Hansen
发明人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g-Hansen
IPC分类号： B01D39/14 , B01D15/02 , B01D15/18 , B01J2/08 , B01J2/16 , B01J8/00 , B01J8/08 , B01J8/18 , B01J8/20 , B01J8/22 , B01J8/24 , B01J8/32 , B01J13/00 , B01J13/02 , B01J20/22 , B01J20/28 , B01J20/281 , B01J20/283 , B01J20/284 , B01J20/285 , B01J20/32 , B01J47/10 , C02F1/28 , C02F1/72 , C02F3/10 , C02F3/12 , C02F3/34 , C07B63/00 , C08K3/00 , C08K3/22 , C08K5/00 , C08L101/00 , C12M1/40 , G01N30/58 , G01N30/88 , C12N11/00 , B01D15/00 , C02F1/00 , C07K1/16
CPC分类号： B01J20/28019 , B01D15/1807 , B01J13/02 , B01J2/16 , B01J20/28004 , B01J20/28011 , B01J20/28014 , B01J20/28021 , B01J20/28026 , B01J20/2803 , B01J20/3219 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J20/3274 , B01J47/10 , B01J8/008 , B01J8/085 , B01J8/1845 , B01J8/1872 , B01J8/1881 , B01J8/20 , B01J8/22 , B01J8/32 , C02F1/286 , C02F1/722 , C02F3/085 , C02F3/10 , C02F3/108 , C02F3/342 , B01D2215/021 , Y02W10/15 , Y10S435/815 , Y10S530/811 , Y10S530/812 , Y10S530/813
摘要： In a vertical down-flow fluid bed reactor, suspended particles in liquid proximal to an inlet in an uppermost part of the reactor are agitated to form a downward extending turbulent zone having vigorously moving particles and a non-turbulent zone distal to the inlet having essentially stationary particles in liquid below and adjoining the turbulent zone. In a vertical up-flow fluid bed reactor, an upward extending turbulent zone is formed proximal to an inlet in a lowermost part of the reactor and the non-turbulent zone is above the turbulent zone. The downward or upward extend of the turbulent zone is determined by the degree of agitation. The particles may contain an active substance and be in the form of a conglomerate of base particles having a desired density to control floatation or sedimentation. Particles in the turbulent and non-turbulent zones may be different such as having different specific gravities. Liquid in the reactor may contain an enzyme or microorganism to be immobilized on the particles, or a protein to be purified by binding to the particles. Waste water may be treated in the reactor with particles containing an immobilized enzyme or microorganism, or with ion exchange conglomerate particles.
摘要翻译：在垂直下流流化床反应器中，搅拌反应器最上部的入口附近的液体中的悬浮颗粒以形成具有剧烈移动的颗粒的向下延伸的湍流区域，并且基本上位于入口远侧的非湍流区域静止的颗粒位于液体下方并毗邻湍流区。在垂直向上流动床反应器中，向上延伸的湍流区域形成在反应器的最下部分的入口附近，非湍流区域在湍流区域之上。湍流区的向下或向上延伸由搅拌程度决定。颗粒可以含有活性物质，并且具有具有所需密度的基础颗粒的聚集体的形式，以控制漂浮或沉淀。湍流和非湍流区域中的颗粒可能是不同的，例如具有不同的比重。反应器中的液体可以含有待固定在颗粒上的酶或微生物，或通过与颗粒结合而被纯化的蛋白质。废水可以在反应器中用含有固定化酶或微生物的微粒，或与离子交换砾粒进行处理。

3. 发明授权

US5866006A Coated single particles and their use in fluid bed chromatography 失效
标题翻译：使用涂层颗粒的流化床色谱法
公开(公告)号：US5866006A
公开(公告)日：1999-02-02
申请号：US4316
申请日：1998-01-08
申请人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g Hansen
发明人： Allan Otto Fog Lihme , Claus Schafer Nielsen , Thorkild Christian B.o slashed.g Hansen
IPC分类号： B01D39/14 , B01D15/02 , B01D15/18 , B01J2/08 , B01J2/16 , B01J8/00 , B01J8/08 , B01J8/18 , B01J8/20 , B01J8/22 , B01J8/24 , B01J8/32 , B01J13/00 , B01J13/02 , B01J20/22 , B01J20/28 , B01J20/281 , B01J20/283 , B01J20/284 , B01J20/285 , B01J20/32 , B01J47/10 , C02F1/28 , C02F1/72 , C02F3/10 , C02F3/12 , C02F3/34 , C07B63/00 , C08K3/00 , C08K3/22 , C08K5/00 , C08L101/00 , C12M1/40 , G01N30/58 , G01N30/88 , B01D15/08
CPC分类号： B01J20/28019 , B01D15/1807 , B01J13/02 , B01J2/16 , B01J20/28004 , B01J20/28011 , B01J20/28014 , B01J20/28021 , B01J20/28026 , B01J20/2803 , B01J20/3219 , B01J20/3251 , B01J20/3253 , B01J20/3255 , B01J20/3274 , B01J47/10 , B01J8/008 , B01J8/085 , B01J8/1845 , B01J8/1872 , B01J8/1881 , B01J8/20 , B01J8/22 , B01J8/32 , C02F1/286 , C02F1/722 , C02F3/085 , C02F3/10 , C02F3/108 , C02F3/342 , B01D2215/021 , Y02W10/15 , Y10S435/815 , Y10S530/811 , Y10S530/812 , Y10S530/813
摘要： A fluidized bed chromatographic process for purification and binding of molecules in a liquid by binding the molecules to an active substance covalently bound to chromatographic adsorbent particles in which process a fluidized bed of the adsorbent particles is formed and said liquid is passed through the fluidized bed, wherein the chromatographic adsorbent particles are formed of a porous composite material having pores allowing access to the interior of the composite material by the molecules, and the porous composite material includes: (i) density controlling particles which are either (a) hollow low density particles which are impermeable to the liquid and have a density providing floatation of the adsorbent particles in the liquid or, (b) high density particles having a density providing sedimentation of the adsorbent particles in the liquid; (ii) a matrix formed by consolidating at least one conglomerating agent selected from the group consisting of natural and synthetic organic monomers and polymers; and (iii) an active substance for binding molecules in the liquid; the density controlling particles being embedded in the matrix, and the active substance being covalently bound to the matrix; wherein;the adsorbent particles have a relative density with respect to the liquid which is less than 0.95 or greater than 1.1 and a particle size within the range of 50-750 .mu.m, and each of the relative density and particle size range of the adsorbent particles is selected to provide desired floatation/sedimentation properties of the adsorbent particles in the liquid in the fluidized bed process with substantially no turbulence.
摘要翻译：一种用于通过将分子结合到与色谱吸附剂颗粒共价结合的活性物质来纯化和结合液体中的分子的流化床层析方法，其中形成吸附剂颗粒的流化床并使液体通过流化床，其中色谱吸附剂颗粒包括多孔复合材料，其具有允许通过分子进入复合材料内部的孔，其中每个吸收颗粒的多孔复合材料由以下组成：（i）单一密度控制颗粒，其为（a ）一种中空低密度颗粒，其不透液体并且具有提供吸附剂颗粒在液体中浮选的密度，或（b）具有提供吸附剂颗粒在液体中沉降的密度的高密度颗粒; （ii）通过固结至少一种选自天然和合成有机单体和聚合物的聚集剂形成的基体; 和（iii）用于结合液体中的分子的活性物质; 所述密度控制粒子嵌入所述基质中，所述活性物质与所述基质共价结合; 其中吸附剂颗粒相对于液体的相对密度小于0.95或大于1.1，粒径在1-1000um范围内，并且选择吸附剂颗粒的每个相对密度和粒度范围以在流化床工艺中的液体中提供吸附剂颗粒的期望的浮选/沉降特性，在流化床中基本上没有湍流。

4. 发明授权

US5770416A Permeable hollow particles having an outer shell of mechanically rigid porous material 失效
标题翻译：具有机械刚性多孔材料外壳的可渗透中空颗粒
公开(公告)号：US5770416A
公开(公告)日：1998-06-23
申请号：US070847
申请日：1993-06-03
申请人： Allan Otto Fog Lihme , Thorkild Christian B.o slashed.g-Hansen , Claus Schafer Nielsen
发明人： Allan Otto Fog Lihme , Thorkild Christian B.o slashed.g-Hansen , Claus Schafer Nielsen
IPC分类号： B01J13/02 , B01J20/28 , B01J20/282 , B01J20/32 , G01N33/552 , C12N11/14 , B01J20/10 , C12N5/00
CPC分类号： B01J13/025 , B01J20/041 , B01J20/06 , B01J20/08 , B01J20/10 , B01J20/261 , B01J20/264 , B01J20/28004 , B01J20/28019 , B01J20/28021 , B01J20/2805 , B01J20/282 , B01J20/286 , B01J20/305 , B01J20/3078 , B01J20/3204 , B01J20/3257 , B01J20/3263 , B01J20/3289 , G01N33/552 , B01J2220/54 , B01J2220/58 , Y10S530/811
摘要： Permeable substantially spherical hollow particles of 1-5000 .mu.m in size are formed having an outer shell of a mechanically rigid porous material. Pores of the shell are through-going pores that connect the inside of the particles to surroundings and allow chemical species to traverse the outer shell. To prepare the particles, impermeable hollow particles are treated with an acid or base under reflux conditions to form pores. The outer shell is formed of anhydrous forms of silicon dioxide, metal silicates, metal borosilicates, metal oxides or boric oxide. Metals and metal alloys are not used in forming the outer shell. Particles containing a polymer are formed by immersing the permeable hollow particles in a solution of components that polymerize to form the polymer, allowing the solution to partially fill the particles via the pores and polymerizing the components. The permeable particles are useful as carriers in High Performance Liquid Chromatography, gel filtration, ion exchange, affinity chromatography, immunochemical procedures, solid-phase peptide or protein synthesis, immobilization of catalysts such as enzymes and noble metals and immobilization of cells or virus particles. The particles may also be used as filtration aids, adsorbents or absorbents.
摘要翻译：形成尺寸为1-5000μm的可渗透的基本上为球形的中空颗粒，其具有机械刚性多孔材料的外壳。壳的孔是贯穿的孔，其将颗粒的内部连接到周围，并允许化学物质穿过外壳。为了制备颗粒，在回流条件下用酸或碱处理不渗透的中空颗粒以形成孔。外壳由无水形式的二氧化硅，金属硅酸盐，金属硼硅酸盐，金属氧化物或氧化硼形成。金属和金属合金不用于形成外壳。含有聚合物的颗粒通过将可渗透的中空颗粒浸渍在聚合以形成聚合物的组分的溶液中而形成，使溶液部分地通过孔部填充颗粒并使组分聚合。可渗透颗粒可用作高效液相色谱，凝胶过滤，离子交换，亲和层析，免疫化学方法，固相肽或蛋白质合成，催化剂如酶和贵金属的固定以及细胞或病毒颗粒的固定的载体。颗粒也可用作过滤助剂，吸附剂或吸收剂。

5. 发明授权

US06919436B2 Isolation of proteins 有权
标题翻译：蛋白质的分离
公开(公告)号：US06919436B2
公开(公告)日：2005-07-19
申请号：US10268667
申请日：2002-10-11
申请人： Allan Otto Fog Lihme , Marie Bendix Hansen
发明人： Allan Otto Fog Lihme , Marie Bendix Hansen
IPC分类号： G01N33/53 , A61K39/395 , B01D15/00 , B01D15/18 , B01D15/38 , B01J20/26 , B01J20/32 , C07K1/22 , C07K16/00 , G01N33/531
CPC分类号： C07K16/00 , B01D15/1807 , B01D15/3804 , B01J20/3219 , B01J20/3242 , B01J20/3253 , B01J20/3255 , C07K1/22
摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activiated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.
摘要翻译：本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。杂交瘤细胞培养上清液，动物血浆或血清或初乳。该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如。羧酸。所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

6. 发明授权

US09192907B2 System and method for producing beads 有权
标题翻译：珠粒的制备方法
公开(公告)号：US09192907B2
公开(公告)日：2015-11-24
申请号：US12745611
申请日：2008-12-02
申请人： Allan Otto Fog Lihme , Marie Bendix Hansen , Rene Oehlenschlaeger Holte , Brian Larsen
发明人： Allan Otto Fog Lihme , Marie Bendix Hansen , Rene Oehlenschlaeger Holte , Brian Larsen
IPC分类号： B01J2/08 , B01J13/04
CPC分类号： B01J2/08 , B01J13/046
摘要： The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilizing the droplets by transferring the emulsion from the first reactor to a stabilization reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.
摘要翻译：本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）将（a）包含能够凝胶化的材料的液体组合物组合; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经受凝胶化手段，以在5分钟以内获得凝胶化，并形成珠粒。

7. 发明申请

US20080182979A1 FRACTIONATION OF PROTEIN CONTAINING MIXTURES 有权
标题翻译：含蛋白质的混合物
公开(公告)号：US20080182979A1
公开(公告)日：2008-07-31
申请号：US11943778
申请日：2007-11-21
申请人： Allan Otto Fog Lihme , Marie Bendix Hansen , Inga Vaarst Andersen , Morten Aae Olander
发明人： Allan Otto Fog Lihme , Marie Bendix Hansen , Inga Vaarst Andersen , Morten Aae Olander
IPC分类号： C07K1/22
CPC分类号： B01D15/1807 , A23J1/00 , A23J1/20 , A23J1/205 , A23V2002/00 , B01D15/1871 , B01D15/265 , B01D15/424 , B01J20/28004 , B01J20/28011 , B01J20/28016 , B01J20/28052 , B01J20/286 , B01J20/3268 , B01J20/3293 , A23V2250/54242 , A23V2250/54244 , A23V2250/54248 , A23V2250/5425 , A23V2250/5434 , A23V2300/30
摘要： Thus, a primary aspect of the present invention relates to a method for the fractionation of a protein-containing mixture wherein the protein-containing mixture is selected from the group consisting of milk, milk derived products, milk derived raw materials, vegetable derived products, vegetable derived extracts, fruit derived products, fruit derived extracts, fish derived products, and fish derived extracts, said method comprising the steps of: a) optionally adjusting the pH of the mixture; b) applying said mixture to an adsorption column comprising an adsorbent, said adsorbent comprises a particle with at least one high density non-porous core, surrounded by a porous material, the adsorbent having a particle density of at least 1.5 g/ml and a mean particle size of at most 150 μm; c) optionally washing the column; d) eluting at least one protein from the adsorbent.
摘要翻译：因此，本发明的主要方面涉及一种含蛋白质混合物的分级方法，其中含蛋白质的混合物选自牛奶，奶衍生产品，乳衍生原料，植物衍生产品，所述方法包括以下步骤：a）任选地调节所述混合物的pH值; b）将所述混合物施加到包含吸附剂的吸附塔上，所述吸附剂包括具有至少一个高密度无孔芯的颗粒，被多孔材料包围，所述吸附剂具有至少1.5g / ml的颗粒密度和平均粒径至多150μm; c）任选地洗涤柱; d）从吸附剂中洗脱出至少一种蛋白质。

8. 发明授权

US08815551B2 Production of biofuel and protein from a raw material 有权
标题翻译：从原料生产生物燃料和蛋白质
公开(公告)号：US08815551B2
公开(公告)日：2014-08-26
申请号：US12522885
申请日：2008-01-15
申请人： Allan Otto Fog Lihme
发明人： Allan Otto Fog Lihme
IPC分类号： C12P7/16 , A23J1/00
CPC分类号： C10L1/1802 , B01D15/1807 , C07K1/16 , C12P3/00 , C12P5/023 , C12P7/02 , C12P7/08 , C12P7/16 , C12P7/62 , C12P7/649 , Y02E50/10 , Y02E50/13 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to a method for providing an isolated biofuel and a purified protein product from a raw material suitable for the production of the biofuel or a derivative of said raw material. The method comprises the steps of: (i) subjecting the raw material or a derivative of said raw material to at least one first treatment liberating the biofuel from the raw material or the derivative of said raw material, (ii) isolating the biofuel liberated in step (i) obtaining the isolated biofuel, (iii) subjecting the raw material or a derivative of said raw material to at least one second treatment providing a material suspension, and (iv) subjecting the material suspension from step (iii) to an expanded bed adsorption process obtaining the purified protein product.
摘要翻译：本发明涉及从适合于生产生物燃料的原料或所述原料的衍生物提供分离的生物燃料和纯化的蛋白质产物的方法。该方法包括以下步骤：（i）使所述原料或所述原料的衍生物经受从原料或所述原料的衍生物释放生物燃料的至少一种第一处理，（ii）分离生物燃料中释放的生物燃料步骤（i）获得分离的生物燃料，（iii）使所述原料或所述原料的衍生物经受提供材料悬浮液的至少一次第二处理，和（iv）使来自步骤（iii）的材料悬浮液经膨胀床吸附过程获得纯化的蛋白质产物。

9. 发明授权

US07745582B2 Isolation of immunoglobulins 有权
标题翻译：免疫球蛋白的分离
公开(公告)号：US07745582B2
公开(公告)日：2010-06-29
申请号：US11930556
申请日：2007-10-31
申请人： Allan Otto Fog Lihme , Marie Bendix Hansen
发明人： Allan Otto Fog Lihme , Marie Bendix Hansen
IPC分类号： C07K1/22 , C07K16/00
CPC分类号： C07K16/00 , B01D15/1807 , B01D15/3804 , B01J20/3219 , B01J20/3242 , B01J20/3253 , B01J20/3255 , C07K1/22
摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.
摘要翻译：本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。杂交瘤细胞培养上清液，动物血浆或血清或初乳。该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如，羧酸。所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

10. 发明授权

US06627460B1 Method for preparing water-soluble cross-linked conjugates 有权
标题翻译：制备水溶性交联共轭物的方法
公开(公告)号：US06627460B1
公开(公告)日：2003-09-30
申请号：US09743637
申请日：2001-04-05
申请人： Allan Otto Fog Lihme , Christopher John Stanley
发明人： Allan Otto Fog Lihme , Christopher John Stanley
IPC分类号： G01N33548
CPC分类号： G01N33/531 , G01N33/533 , G01N33/54353 , G01N33/548 , Y10S436/823
摘要： A novel preparative methodology yields water-soluble, cross-linked conjugates and conjugate complexes that confer an improved sensitivity in immunochemical assays, particularly in the context of lateral flow devices and in determinations of the presence or absence of small amounts of active components present in a liquid sample.
摘要翻译：一种新的制备方法产生水溶性交联的缀合物和缀合物复合物，其在免疫化学测定中具有改善的灵敏度，特别是在侧向流动装置的上下文中以及在存在或不存在少量活性成分的情况下液体样品。

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