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1. 发明申请

WO2006107736A1 EXTRACTABILITY AND BIOAVAILABILITY OF THE NATURAL ANTIOXIDANT ASTAXANTHIN FROM A GREEN ALGA, HAEMATOCOCCUS PLUVIALIS 审中-公开
标题翻译：天然抗氧化剂ASTAXANTHIN从绿色ALGA，HAEMATOCOCCUS PLUVIALIS的可提取性和生物利用度
公开(公告)号：WO2006107736A1
公开(公告)日：2006-10-12
申请号：PCT/US2006/011826
申请日：2006-03-31
申请人： ALGAEN CORPORATION , HU, Qiang , SOMMERFELD, Milton, R. , LU, Fan
发明人： HU, Qiang , SOMMERFELD, Milton, R. , LU, Fan
IPC分类号： A01N61/00 , A01N65/00 , A61K31/00 , A61K36/00 , A01H13/00
CPC分类号： A61K36/05 , A23K10/12 , A23K20/105 , A23K50/10 , A23K50/30 , A23K50/75 , A23K50/80 , A23L17/60 , A61K31/00
摘要： As the richest source of astaxanthin, a natural antioxidant and coloring agent, the unicellular green alga, Haematococcus pluvialis, is being commercially exploited. A major constraint in the Haematococcus production system, however, is the thick, rigid cell walls associated with astaxanthin-rich cysts (or aplanospores). The thick walls prevent the extraction of cellular materials and consequently reduce the bioavailability of astaxanthin. Using a physical, chemical, or enzymatic method to disrupt the cell wall has proven to be very expensive and also introduce the risk of oxidation of astaxanthin by atmospheric oxygen The present invention provides a novel method for solving this problem by introducing two genetically modified Haematococcus pluvialis mutants. These two mutants, named as D 13- 17 and N54-22, contain remarkably reduced amounts of cell wall materials, but retain the growth potential and ability to accumulate astaxanthin as high as the wild type strain. Organic solvent extraction efficiency assay has demonstrated that cellular astaxanthin can be more effectively and efficiently extracted from the cell wall-deficient mutants than from the wild type, suggesting that the mutants may provide better bioavailability of astaxanthin to humans and animals. The said mutants can be used for production of natural astaxanthin for human and animal consumption
摘要翻译：作为天然抗氧化剂和着色剂的虾青素最丰富的来源，单细胞绿藻，红球藻（Haematococcus pluvialis）正在商业上被利用。然而，血球菌生产系统的主要制约因素是与富含虾青素的囊肿（或淀粉孢子）相关的厚而刚性的细胞壁。厚壁防止细胞材料的提取，从而降低虾青素的生物利用度。使用物理，化学或酶学方法破坏细胞壁已被证明是非常昂贵的，并且还引起大气氧氧化虾青素的风险本发明提供了一种通过引入两种遗传修饰的红潮球菌（Hematococcus pluvialis）来解决这个问题的新方法突变体。这两个突变体，命名为D 13-17和N54-22，含有显着减少量的细胞壁材料，但保留生长潜力和积累与野生型菌株一样高的虾青素的能力。有机溶剂提取效率测定表明，从野生型中可以更有效地从细胞壁缺陷突变体提取细胞虾青素，这表明突变体可以提供更好的虾青素对人和动物的生物利用度。所述突变体可用于生产天然虾青素用于人和动物消费

2. 发明申请

WO2005074622A2 COLONIES OF NOSTOC COMMUNE, METHODS FOR CULTIVATING EDIBLE NOSTOC COMMUNE AND EDIBLE NOSTOC COMMUNE FORMULATIONS AND THEIR USE FOR PROMOTING HEALTH 审中-公开
标题翻译： NOSTOC社区组织，可口可乐社区和食用NOSTOC社区公约的组织方法及其促进健康的使用
公开(公告)号：WO2005074622A2
公开(公告)日：2005-08-18
申请号：PCT/US2005/003314
申请日：2005-02-03
申请人： ALGAEN CORPORATION , LU, Fan , HU, Qiang , HU, Zheng, Yu , DENG, Zhong, Yang
发明人： LU, Fan , HU, Qiang , HU, Zheng, Yu , DENG, Zhong, Yang
IPC分类号： A61K35/748
CPC分类号： C12N1/20 , A01H4/001 , A23L33/135 , A61K35/748 , A61K45/06 , C12M21/02 , C12M23/06 , C12M23/48 , C12M23/52 , C12N13/00
摘要： Nostoc formulations, dietary supplements comprising the Nostoc formulations, and food products comprising Nostoc commune, (also known as Nostoc sphaericum, or Nostoc commune var. sphaericum) are disclosed. Nostoc formulations that may additionally comprise a medicinal composition are described. The present invention further relates to processes for producing these Nostoc formulations. In addition, the present invention relates to methods for promoting the health of an individual utilizing the Nostoc formulations, dietary supplements, food products and/or pharmacological compositions of the present invention. Moreover, this invention provides a method for cultivating Nostoc commune comprising (a). isolating and purifying Nostoc commune; (b). culturing the Nostoc commune; and (c). conditions suitable for optimal growth of Nostoc commune.
摘要翻译： Nostoc制剂，包含Nostoc制剂的膳食补充剂和包含Nostoc commune（也称为Nostoc sphaericum）或Nostoc commune var。sphaericum的食品。描述了可另外包含药物组合物的Nostoc制剂。本发明还涉及制备这些Nostoc制剂的方法。此外，本发明涉及利用本发明的Nostoc制剂，膳食补充剂，食品和/或药物组合物促进个体健康的方法。此外，本发明提供了一种培养包括（a）的Nostoc公社的方法。分离和净化Nostoc公社; （b）中。培养Nostoc公社; 和（c）。适合Nostoc公社最佳生长条件。

3. 发明公开

EP1753857A2 COLONIES OF NOSTOC COMMUNE, METHODS FOR CULTIVATING EDIBLE NOSTOC COMMUNE AND EDIBLE NOSTOC COMMUNE FORMULATIONS AND THEIR USE FOR PROMOTING HEALTH 审中-公开
标题翻译：发菜协会，法栽培食用菌发菜协会和食用发菜协会制剂和它们使用的健康KOLONIEN
公开(公告)号：EP1753857A2
公开(公告)日：2007-02-21
申请号：EP05722689.6
申请日：2005-02-03
申请人： Algaen Corporation
发明人： LU, Fan , HU, Qiang , HU, Zheng, Yu , DENG, Zhong, Yang
IPC分类号： C12N1/00
CPC分类号： C12N1/20 , A01H4/001 , A23L33/135 , A61K35/748 , A61K45/06 , C12M21/02 , C12M23/06 , C12M23/48 , C12M23/52 , C12N13/00
摘要： Nostoc formulations, dietary supplements comprising the Nostoc formulations, and food products comprising Nostoc commune, (also known as Nostoc sphaericum, or Nostoc commune var. sphaericum) are disclosed. Nostoc formulations that may additionally comprise a medicinal composition are described. The present invention further relates to processes for producing these Nostoc formulations. In addition, the present invention relates to methods for promoting the health of an individual utilizing the Nostoc formulations, dietary supplements, food products and/or pharmacological compositions of the present invention. Moreover, this invention provides a method for cultivating Nostoc commune comprising (a). isolating and purifying Nostoc commune; (b). culturing the Nostoc commune; and (c). conditions suitable for optimal growth of Nostoc commune.

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