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1. 发明授权

US4782022A Nitrogen fixation regulator genes 失效
标题翻译：氮固定调节基因
公开(公告)号：US4782022A
公开(公告)日：1988-11-01
申请号：US737029
申请日：1985-05-22
申请人： Alfred Puhler , Helmut Reilaender , Gerhard Weber
发明人： Alfred Puhler , Helmut Reilaender , Gerhard Weber
IPC分类号： C12N15/00 , C07H15/12 , C12N1/20
CPC分类号： C12N15/00
摘要： Isolation and characterization of a gene which activated nitrogen fixation genes of Rhizobium meliloti when that bacterium is in a symbiotic relationship with a plant is disclosed. This newly discovered gene, designated fix D, can activate the nifHD promoter. A method of making this inducible gene constitutive is presented. This is useful for making nifHD constitutive. The combination of the fixD promoter with heterologous structural genes is taught. Such combinations are useful for limiting expression of an encoded protein to rhizobia involved in a symbiotic relationship with a plant. Plasmids and methods useful in performance of this invention are also disclosed.
摘要翻译：公开了当该细菌与植物共生关系时活化根瘤菌根瘤菌的固氮基因的基因的分离和表征。这个新发现的基因，命名为固定物D，可以激活nifHD启动子。提出了一种制作这种诱导型基因本构的方法。这对于制造nifHD本构是有用的。教导了fixD启动子与异源结构基因的组合。这样的组合可用于将编码的蛋白质的表达限制为与植物共生关系参与的根瘤菌。还公开了用于实施本发明的质粒和方法。

2. 发明授权

US5346818A Method for the conjugative transfer of mobilizable vectors for E. coli to gram-positive bacteria and vectors suitable for use in such a method 失效
标题翻译：将用于大肠杆菌的可动员载体共轭转移到革兰氏阳性细菌和适用于这种方法的载体的方法
公开(公告)号：US5346818A
公开(公告)日：1994-09-13
申请号：US447139
申请日：1989-12-08
申请人： Andreas Schafer , Jorn Kalinowski , Alfred Puhler
发明人： Andreas Schafer , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N15/09 , C12N1/21 , C12N15/74 , C12N15/77 , C12N15/00 , C12N15/63
CPC分类号： C12N15/74 , C12N15/77
摘要： The present invention relates to a method of effecting the conjugative transfer of a mobilizable vector from cells of an E. coli mobilizer strain to gram-positive bacterial cells. The invention also relates to vectors suitable for use in such a method.
摘要翻译：本发明涉及一种将可移动载体从大肠杆菌移动剂菌株的细胞共轭转移到革兰氏阳性细菌细胞的方法。本发明还涉及适用于这种方法的载体。

3. 发明授权

US4965207A Color marker for clonings in Streptomyces lividans 失效
标题翻译：链霉菌（Streptomyces lividans）中克隆的颜色标记
公开(公告)号：US4965207A
公开(公告)日：1990-10-23
申请号：US83805
申请日：1987-08-11
申请人： Gunter Muth , Wolfgang Wohlleben , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt
发明人： Gunter Muth , Wolfgang Wohlleben , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt
IPC分类号： C12N15/09 , C07K14/36 , C12N1/21 , C12N15/00 , C12N15/65 , C12N15/76 , C12P21/00 , C12R1/465
CPC分类号： C12N15/76 , C07K14/36 , C12N15/65 , Y10S435/886
摘要： Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
摘要翻译：通过总DNA的全部消化，克隆到合适的载体和变形链霉菌（Streptomyces lividans）中，从Streptomyces coelicolor DSM 3030获得强烈的深红色菌落。重新分离质粒DNA并用BamHI切割产生长度约3.4至9kb的片段，其位于确定着色剂生产的基因。该基因在链霉菌中作为标记物，特别是作为灭活标记物是合适的。

4. 发明授权

US4963496A Color marker in streptomycetes plasmids 失效
标题翻译：链霉菌质粒中的色标
公开(公告)号：US4963496A
公开(公告)日：1990-10-16
申请号：US83806
申请日：1987-08-11
申请人： Gunter Muth , Wolfgang Wohlleben , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt
发明人： Gunter Muth , Wolfgang Wohlleben , Alfred Puhler , Gerhard Wohner , Rudiger Marquardt
IPC分类号： C12N15/09 , C12N1/21 , C12N15/00 , C12N15/65 , C12N15/76 , C12P21/00 , C12R1/465
CPC分类号： C12N15/76 , C12N15/65
摘要： Intensely blue-black colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of a streptomycetes recipient strain. Re-isolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the gene for the coloring agent is located. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
摘要翻译：通过总DNA的总消化，克隆到合适的载体和链霉菌受体菌株的转化中，从Streptomyces coelicolor DSM 3030获得强烈的蓝黑色菌落。重新分离质粒DNA并用BamHI切割产生5.5kb片段，其上着色剂的基因位于其上。该基因在链霉菌中作为标记物，特别是作为灭活标记物是合适的。

5. 发明授权

US4626504A DNA transfer vector for gram-negative bacteria 失效
标题翻译：革兰氏阴性细菌的DNA转移载体
公开(公告)号：US4626504A
公开(公告)日：1986-12-02
申请号：US510370
申请日：1983-07-01
申请人： Alfred Puhler , Reinhard Simon
发明人： Alfred Puhler , Reinhard Simon
IPC分类号： C12N15/74 , C12N15/00 , C12N1/00 , C12N1/20
CPC分类号： C12N15/74 , C12N15/743
摘要： Modified E. coli vectors are disclosed that are mobilizable to a wide range of gram-negative bacteria, but are not self-transmissible. The modified E. coli plasmids replicate only in bacterial strains of the E. coli group. Mobilizer strains of E. coli are also provided, as well as methods for employing the modified E. coli vectors for transposon mutagenesis, site-specific gene transfer, and the construction of DNA libraries.
摘要翻译：公开了修饰的大肠杆菌载体，其可移动到广泛的革兰氏阴性细菌，但不是自传播的。修饰的大肠杆菌质粒仅在大肠杆菌组的细菌菌株中复制。还提供了大肠杆菌的动员菌株，以及使用修饰的大肠杆菌载体用于转座子诱变，位点特异性基因转移和DNA文库构建的方法。

6. 发明申请

US20050196848A1 Process for the fermentative production of L-amino acids by attenuation of the poxB gene 审中-公开
标题翻译：通过痘痘基因的减毒发酵生产L-氨基酸的方法
公开(公告)号：US20050196848A1
公开(公告)日：2005-09-08
申请号：US11111831
申请日：2005-04-22
申请人： Nicole Dusch , Brigitte Bathe , Jorn Kalinowski , Alfred Puhler , Bettina Mockel , Anke Weissenborn , Walter Pfefferle
发明人： Nicole Dusch , Brigitte Bathe , Jorn Kalinowski , Alfred Puhler , Bettina Mockel , Anke Weissenborn , Walter Pfefferle
IPC分类号： C12N15/09 , C12N1/21 , C12N9/02 , C12N15/31 , C12N15/53 , C12P13/08 , C12R1/15 , C12Q1/68 , C07H21/04 , C12N1/20 , C12N9/10 , C12N15/74
CPC分类号： C12N9/0008
摘要： An isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide containing at least 15 successive bases of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids by attenuation of the poxB gene.
摘要翻译：一种分离的多核苷酸，其含有多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码含有SEQ ID NO：1的氨基酸序列的多肽的多核苷酸至少70％相同。 2，b）编码多肽的多核苷酸，其含有与SEQ ID No：1的氨基酸序列至少70％相同的氨基酸序列。 2）c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）多核苷酸序列的至少15个连续碱基的多核苷酸，以及发酵生产L - 氨基酸通过减少poxB基因。

7. 发明申请

US20050100927A1 Nucleotide sequence encoding the dapC gene and process for the production of L-lysine 有权
标题翻译：编码dapC基因的核苷酸序列和生产L-赖氨酸的方法
公开(公告)号：US20050100927A1
公开(公告)日：2005-05-12
申请号：US10745684
申请日：2003-12-29
申请人： Bettina Mockel , Anke Weissenborn , Walter Pfefferle , Michael Hartmann , Jorn Kalinowski , Alfred Puhler
发明人： Bettina Mockel , Anke Weissenborn , Walter Pfefferle , Michael Hartmann , Jorn Kalinowski , Alfred Puhler
IPC分类号： C12N15/09 , C12N1/21 , C12N9/10 , C12N15/31 , C12N15/54 , C12P13/08 , C12Q1/68 , C12R1/13
CPC分类号： C12P13/08 , C12N9/1096
摘要： The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.
摘要翻译：本发明涉及来自棒状细菌的分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码含有根据SEQ ID No.3所述的氨基酸序列的多肽的多核苷酸至少70％相同。 2，b）编码多肽的多核苷酸，其含有与SEQ ID NO：1的氨基酸序列至少70％相同的氨基酸序列。 2）c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及d）发酵生产的方法的L-氨基酸，特别是L-赖氨酸。

8. 发明授权

US06787334B1 Process for the preparation of pantothenic acid by amplification of nucleotide sequences which code for the ketopantoate reductase 有权
标题翻译：通过扩增编码酮聚糖还原酶的核苷酸序列来制备泛酸的方法
公开(公告)号：US06787334B1
公开(公告)日：2004-09-07
申请号：US09680310
申请日：2000-10-06
申请人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
发明人： Frank Elischweski , Jorn Kalinowski , Alfred Puhler , Nicole Dusch , Jurgen Dohmen , Mike Farwick , Georg Thierbach
IPC分类号： C12P2106
CPC分类号： C12N9/0008 , C12P7/42
摘要： The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.
摘要翻译：本发明涉及通过扩增编码酮戊酸还原酶，特别是panE基因的核苷酸序列来单独地或彼此组合并任选地另外添加ilvC来制备和改善D-泛酸产生微生物的方法基因，含有这些核苷酸序列的微生物，以及D-泛酸的制备方法，其包括这些微生物的发酵，在培养基中或微生物细胞中浓缩泛酸，以及分离D-泛酸。

9. 发明授权

US06184007B2 Method for the fermentative production of D-pantothenic acid by enhancement of the panD gene in microorganisms 失效
标题翻译：通过增加微生物中panD基因来发酵D-泛酸的方法
公开(公告)号：US06184007B2
公开(公告)日：2001-02-06
申请号：US09318793
申请日：1999-05-26
申请人： Nicole Dusch , Jörn Kalinowski , Alfred Puhler
发明人： Nicole Dusch , Jörn Kalinowski , Alfred Puhler
IPC分类号： C12P1300
CPC分类号： C12N9/88 , C12N15/52 , C12P13/02
摘要： The invention is a method for the production of D-pantothenic acid by the fermentation of microorganisms in which at least the panD gene is enhanced (overexpressed), optionally in combination with the panB and/or panC gene, to provide for the enrichment of the pantothenic acid in the medium or the cells of the microorganisms.
摘要翻译：本发明是通过微生物的发酵来生产D-泛酸的方法，其中至少panD基因被增强（过表达），任选与panB和/或panC基因组合，以提供丰富的培养基中的泛酸或微生物的细胞。

10. 发明授权

US4673642A Plasmid P SVH 1 and its use 失效
标题翻译：质粒P SVH 1及其用途
公开(公告)号：US4673642A
公开(公告)日：1987-06-16
申请号：US642742
申请日：1984-08-20
申请人： Alfred Puhler , Wolfgang Wolleben , Michael Leineweber
发明人： Alfred Puhler , Wolfgang Wolleben , Michael Leineweber
IPC分类号： C12N15/09 , C07H21/04 , C12N15/76 , C12P19/34 , C12R1/465 , C12R1/61 , C12N1/00 , C12N1/20 , C12N15/00 , C12P21/00
CPC分类号： C12N15/76 , Y10S435/886 , Y10S435/906
摘要： The plasmid p SVH 1, which is obtained from Streptomyces venezuelae DSM 40755 and which has a molecular weight of 8.4 megadaltons, a contour length of 4.1 .mu.m and a molecular size of 12.6 kilobases, and its use for the construction of a vector and for cloning foreign DNA in suitable host organisms.
摘要翻译：得自Streptomyces venezuelae DSM 40755，分子量为8.4兆道尔顿，轮廓长度为4.1μm，分子大小为12.6千碱基的质粒p SVH 1及其用于构建载体和在适当的宿主生物体内克隆外来DNA。

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