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1. 发明授权

US08916363B2 Construction of Highly efficient cellulase compositions for enzymatic hydrolysis of cellulose 有权
标题翻译：构建纤维素酶水解的高效纤维素酶组合物
公开(公告)号：US08916363B2
公开(公告)日：2014-12-23
申请号：US12908454
申请日：2010-10-20
申请人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
发明人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
IPC分类号： C12P19/22 , C12P19/14 , C12N9/42
CPC分类号： C12N9/2437 , C12N9/244 , C12N9/2445 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.
摘要翻译：本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述）作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种其他。酶组合物在木质纤维素生物质的水解中是有效的。

2. 发明授权

US08673618B2 Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose 有权
标题翻译：构建用于纤维素酶水解的高效纤维素酶组合物
公开(公告)号：US08673618B2
公开(公告)日：2014-03-18
申请号：US12908483
申请日：2010-10-20
申请人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
发明人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
IPC分类号： C12N1/15
CPC分类号： C12N9/2437 , C12N9/244 , C12N9/2445 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.
摘要翻译：本发明提供使用新鉴定和分离的C.运气型酶的新型酶组合物，包括CBH Ib CBH IIb，EG II，EG VI，β-葡糖苷酶和木聚糖酶II结合先前鉴定的酶CBH 1a，CBH IIa（以前描述为 Endo 43）和EG V.这些酶组合物表现出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，Douglas冷杉木材）转化为葡萄糖的极高的能力。两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种其他。酶组合物在木质纤维素生物质的水解中是有效的。

3. 发明授权

US07883872B2 Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose 有权
标题翻译：构建用于纤维素酶水解的高效纤维素酶组合物
公开(公告)号：US07883872B2
公开(公告)日：2011-02-08
申请号：US11487547
申请日：2006-07-13
申请人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
发明人： Alexander V. Gusakov , Tatyana N. Salanovich , Alexey I. Antonov , Boris B. Ustinov , Oleg N. Okunev , Richard P. Burlingame , Mark A. Emalfarb , Marco A. Baez , Arkady P. Sinitsyn
IPC分类号： C12P19/16 , C12P19/20
CPC分类号： C12N9/2437 , C12N9/244 , C12N9/2445 , C12P19/14 , C12Y302/01004 , C12Y302/01006 , C12Y302/01021 , C12Y302/01091 , Y02E50/16
摘要： This invention provides novel enzyme compositions using newly identified and isolated C. lucknowense enzymes, including CBH Ib CBH IIb, EG II, EG VI, β-glucosidase, and xylanase II in conjunction with previously identified enzymes CBH Ia, CBH IIa (previously described as Endo 43), and EG V. These enzyme compositions demonstrate an extremely high ability to convert lignocellulosic biomass (e.g., Avicel, cotton, Douglas fir wood pretreated by organosolv) to glucose. CBH Ia and IIb, which both have a cellulose-binding module (CBM) displayed a pronounced synergism with three major endoglucanases (EG II, EG V, EG VI) from the same fungus in hydrolysis of cotton as well as a strong synergy with each other. The enzyme compositions are effective in hydrolysis of the lignocellulosic biomass.
摘要翻译：本发明提供使用新鉴定和分离的C.幸运感酶的新型酶组合物，其包括CBH Ib CBH IIb，EG II，EG VI，葡萄糖苷酶和木聚糖酶II与先前鉴定的酶CBH 1a，CBH IIa（先前描述）作为Endo 43）和EG V.这些酶组合物显示出将木质纤维素生物质（例如，通过有机溶剂预处理的Avicel，棉花，道格拉斯冷杉木材）转化为葡萄糖的极高的能力。两者具有纤维素结合模块（CBM）的CBH 1a和IIb显示出与来自棉花水解的相同真菌的三种主要内切葡聚糖酶（EG II，EG V，EG VI）的显着协同作用，以及与每种其他。酶组合物在木质纤维素生物质的水解中是有效的。

4. 发明授权

US08680252B2 Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi 有权
标题翻译：丝状真菌中复合表达DNA文库的表达和高通量筛选
公开(公告)号：US08680252B2
公开(公告)日：2014-03-25
申请号：US12518595
申请日：2007-12-10
申请人： Mark A. Emalfarb , Peter J. Punt , Cornelia Van Zeijl , Cornelius Van Den Hondel , Jan Verdoes , Richard P. Burlingame
发明人： Mark A. Emalfarb , Peter J. Punt , Cornelia Van Zeijl , Cornelius Van Den Hondel , Jan Verdoes , Richard P. Burlingame
IPC分类号： C12P1/00 , C12P21/04 , C07H21/02
CPC分类号： C12N15/80 , C07K16/00 , C07K2317/14 , C07K2319/30 , C12N9/58 , C12N15/1082 , C12P21/02 , C12R1/645 , C12Y204/0201 , C12Y401/01023
摘要： The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.
摘要翻译：本发明提供了在丝状真菌宿主中表达和随后筛选DNA文库，特别是合成的，基因组和cDNA文库的方法。特别地，本发明提供了载体，宿主菌株和用于表达和筛选复合DNA文库的方法，包括但不限于表达一种，两种或更多种可变成分和/或由两种或两种以上制备的组合（组合）文库或更多的亚图书馆（例如，用于表达和筛选免疫球蛋白（包括整个免疫球蛋白蛋白的片段和衍生物）和其他受体或复合DNA文库或文库的文库）。本发明可用于表达和筛选大量蛋白质和蛋白质复合物，包括人类蛋白质。本发明还涉及新的真菌蛋白酶序列。

5. 发明申请

US20120030839A1 Expression and High-Throughput Screening of Complex Expressed DNA Libraries in Filamentous Fungi 有权
标题翻译：表达和高通量筛选复合表达的DNA文库在丝状真菌
公开(公告)号：US20120030839A1
公开(公告)日：2012-02-02
申请号：US12518595
申请日：2007-12-10
申请人： Mark A. Emalfarb , Peter J. Punt , Cornelia Van Zeijl , Cornelius Van Den Hondel , Jan Verdoes , Richard P. Burlingame
发明人： Mark A. Emalfarb , Peter J. Punt , Cornelia Van Zeijl , Cornelius Van Den Hondel , Jan Verdoes , Richard P. Burlingame
IPC分类号： A01H5/00 , C12N1/15 , C12N1/14 , C40B50/06 , C40B20/00 , C40B30/00 , C40B30/04 , C12N1/21 , C12N9/58 , C12N15/80 , C12N15/85 , C12N15/81 , C40B10/00 , C40B40/02 , A61K39/00 , C12P21/00 , C07H21/04 , C07K16/40 , C12P21/06 , C12N15/00 , A61P37/04 , C12N15/63
CPC分类号： C12N15/80 , C07K16/00 , C07K2317/14 , C07K2319/30 , C12N9/58 , C12N15/1082 , C12P21/02 , C12R1/645 , C12Y204/0201 , C12Y401/01023
摘要： The invention provides a method for the expression and subsequent screening of DNA libraries, particularly synthetic, genomic, and cDNA libraries, in filamentous fungal hosts. In particular, the invention provides vectors, host strains, and a method for the expression and screening of complex DNA libraries, including, but not limited to, combinatory (combinatorial) libraries expressing one, two or more variable constituents and/or prepared from two or more sublibraries (e.g., for the expression and screening of immunoglobulin (including fragments and derivatives of whole immunoglobulin proteins) and other receptor or complex DNA libraries or libraries of libraries). The invention is useful for the expression and screening for a large variety of proteins and protein complexes, including human proteins. The present invention also relates to novel fungal protease sequences.
摘要翻译：本发明提供了在丝状真菌宿主中表达和随后筛选DNA文库，特别是合成的，基因组和cDNA文库的方法。特别地，本发明提供了载体，宿主菌株和用于表达和筛选复合DNA文库的方法，包括但不限于表达一种，两种或更多种可变成分和/或由两种或两种以上制备的组合（组合）文库或更多的亚图书馆（例如，用于表达和筛选免疫球蛋白（包括整个免疫球蛋白蛋白的片段和衍生物）和其他受体或复合DNA文库或文库的文库）。本发明可用于表达和筛选大量蛋白质和蛋白质复合物，包括人类蛋白质。本发明还涉及新的真菌蛋白酶序列。

6. 发明授权

US08609371B2 Isoprenoid compounds 有权
标题翻译：异戊二烯类化合物
公开(公告)号：US08609371B2
公开(公告)日：2013-12-17
申请号：US12540050
申请日：2009-08-12
申请人： Bryan Julien , Richard P. Burlingame
发明人： Bryan Julien , Richard P. Burlingame
IPC分类号： C12P21/00 , C12P19/34 , C12P5/00 , C07H21/04
CPC分类号： C12N9/1085 , C12N15/1079 , C12P5/002 , C12P5/007
摘要： The present invention is directed to variant squalene synthase enzymes, including Saccharomyces cerevisiae squalene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow the S. cerevisiae cells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.
摘要翻译：本发明涉及变体角鲨烯合酶，包括酿酒酵母角鲨烯合酶，以及编码这些变体酶的核酸分子。这些变体酶以比野生型酶低的速率产生角鲨烯，允许更多的法呢基焦磷酸盐用于生产异戊二烯化合物，同时仍然产生足够的角鲨烯以允许酿酒酵母细胞生长而不需要由甾醇补充如麦角甾醇。因此，这些变体酶非常适合有效生产类异戊二烯。

7. 发明申请

US20120246767A1 Modified valencene synthase polypeptides, encoding nucleic acid molecules and uses thereof 有权
标题翻译：编码核酸分子的改良的戊烯合酶多肽及其用途
公开(公告)号：US20120246767A1
公开(公告)日：2012-09-27
申请号：US13317839
申请日：2011-10-28
申请人： Jean Davin Amick , Eunyoung Park , Bryan N. Julien , Richard P. Burlingame
发明人： Jean Davin Amick , Eunyoung Park , Bryan N. Julien , Richard P. Burlingame
IPC分类号： C12N9/00 , C12N15/63 , C12N1/21 , C12P7/26 , C12N5/10 , A01H5/00 , C12P5/00 , C12N15/52 , C12N1/19
CPC分类号： C12N9/88 , C07H21/04 , C07K14/415 , C12N15/81 , C12N15/82 , C12N15/8243 , C12N15/86
摘要： Provided are modified valencene synthase polypeptides and methods of using the modified valencene synthase polypeptides. Also provided are methods for producing modified terpene synthases.
摘要翻译：提供了修饰的戊烯合酶多肽和使用修饰的戊烯合酶多肽的方法。还提供了用于生产改性萜烯合酶的方法。

8. 发明授权

US5856148A Materials and methods for biosynthesis of serine and serine-related products 失效
标题翻译：生物合成丝氨酸和丝氨酸相关产物的材料和方法
公开(公告)号：US5856148A
公开(公告)日：1999-01-05
申请号：US797727
申请日：1997-02-11
申请人： Richard P. Burlingame
发明人： Richard P. Burlingame
IPC分类号： C12N9/04 , C12N15/53
CPC分类号： C12N9/0006
摘要： Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.
摘要翻译：与野生型PGD相比，编码3-磷酸甘油酸脱氢酶（PGD）的工程DNA降低了对丝氨酸抑制的敏感性。 DNA编码PGD，其具有至少一些可用于生物合成的酶活性水平，并且在野生型（未修饰的）野生型PGD中保留比丝氨酸水平更高的活性。 PGD具有上述工程DNA的氨基酸序列。表达载体含有这种工程化的DNA和调节DNA，其定位并定向于在宿主表达系统中表达工程化的DNA。细胞含有这些表达载体，制备丝氨酸或丝氨酸衍生产物的方法包括培养这些细胞。

9. 发明授权

US5618716A Materials and methods for biosynthesis of serine and serine-related products 失效
标题翻译：生物合成丝氨酸和丝氨酸相关产物的材料和方法
公开(公告)号：US5618716A
公开(公告)日：1997-04-08
申请号：US244491
申请日：1994-05-24
申请人： Richard P. Burlingame
发明人： Richard P. Burlingame
IPC分类号： C12N1/19 , C12N1/21 , C12N9/04 , C12N15/09 , C12N15/53 , C12P13/06 , C12P13/22 , C12R1/19 , C12R1/865
CPC分类号： C12N9/0006 , C12P13/06
摘要： Engineered DNA encoding 3-phosphoglycerate dehydrogenate (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. The PGD has the amino acid sequence of this above-described engineered DNA. Expression vectors contain this engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system. Cells contain these expression vectors, and a method for producing serine or a serine-derived product includes culturing such cells.
摘要翻译： PCT No.PCT / EP92 / 01873 Sec。 371日期：1994年5月24日 102（e）日期1994年5月24日PCT提交1992年8月17日PCT公布。出版物WO93 / 12235 日期1993年6月24日编号3-磷酸甘油酸脱氢酶（PGD）的工程DNA比野生型PGD降低了对丝氨酸抑制的敏感性。 DNA编码PGD，其具有至少一些可用于生物合成的酶活性水平，并且在野生型（未修饰的）野生型PGD中保留比丝氨酸水平更高的活性。 PGD具有上述工程DNA的氨基酸序列。表达载体含有这种工程化的DNA和调节DNA，其定位并定向于在宿主表达系统中表达工程化的DNA。细胞含有这些表达载体，制备丝氨酸或丝氨酸衍生产物的方法包括培养这些细胞。

10. 发明授权

US07892812B2 Chrysosporium cellulase and methods of use 有权
标题翻译：菊苣纤维素酶及其使用方法
公开(公告)号：US07892812B2
公开(公告)日：2011-02-22
申请号：US09284152
申请日：1997-09-30
申请人： Mark Aaron Emalfarb , Irina Vladimirovna Solovjeva , Arie Ben-Bassat , Richard P. Burlingame , Vladimir Mikhaylovich Chernoglazov , Oleg Nicolaevich Ocounev , Philip T. Olson , Arkady Panteleimonovich Sinitsyn
发明人： Mark Aaron Emalfarb , Irina Vladimirovna Solovjeva , Arie Ben-Bassat , Richard P. Burlingame , Vladimir Mikhaylovich Chernoglazov , Oleg Nicolaevich Ocounev , Philip T. Olson , Arkady Panteleimonovich Sinitsyn
IPC分类号： C09B67/00 , C12N9/00 , C12N9/42 , C12N1/00 , D21C1/00
CPC分类号： D06P5/158 , C11D3/38645 , C11D11/0017 , C12N9/2437 , C12R1/645 , C12Y302/01004 , D06L4/40 , D06M16/003 , D06M2101/06 , D06P5/02
摘要： A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (116). Work processes are modeled using an application framework (99) used to develop abstract, decision (100) process models. The decision (100) process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants (116) in the work process.
摘要翻译：本发明涉及中和和/或碱性纤维素酶的新型组合物和从Chrysosporium培养物，特别是Chrysosporium lucknowense获得中性和/或碱性纤维素酶组合物的方法。本发明还提供了产生能够产生中性和/或碱性纤维素酶的Chrysosporium突变体的突变体和方法。本发明还涉及编码包含中性和/或碱性纤维素酶组合物的酶的基因。此外，本发明提供培养Chrysosporium以产生中性和/或碱性纤维素酶的方法。本发明的中性和/或碱性纤维素酶组合物可以用于各种工艺，包括洗衣服，洗涤剂工艺，脱墨，增色，脱色和纸和纸浆的生物漂白以及处理废物流。本发明还涉及具有葡聚糖酶和纤维二糖水解酶活性的纤维素酶的分离和纯化，并且可用于石洗应用。

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