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    • 9. 发明申请
    • METHODS OF TRANSPORTING EPITHELIAL CELL MONOLAYERS
    • 运输上皮细胞单层膜的方法
    • US20100047907A1
    • 2010-02-25
    • US12544118
    • 2009-08-19
    • Jibin LiPaula S. KardosSamantha Mackenzie AllenYing Wang
    • Jibin LiPaula S. KardosSamantha Mackenzie AllenYing Wang
    • C12N5/08
    • C12M25/04
    • Described herein are tissue culture plates with permeable tissue culture plate inserts therein, which provides the tissue culture plates with apical chamber and a basolateral chambers, wherein cells are deposited on the permeable tissue culture inserts and essentially all of tissue culture medium has been removed from the apical chambers of the tissue culture plates and the basolateral chambers of the tissue culture plates contain a solidifiable form of cell culture medium. Also described are cells that can be deposited and grown on the described tissue culture inserts, methods for transporting a tissue culture plate with a permeable tissue culture plate insert therein on which cells are deposited. Also described is a kit for transporting the tissue culture plates described above, and corresponding methods of use.
    • 本文描述的是其中具有可渗透组织培养板插入物的组织培养板,其向组织培养板提供顶端室和基底外侧室,其中细胞沉积在可渗透组织培养插入物上,并且基本上所有的组织培养基已经从 组织培养板的顶端室和组织培养板的基底外侧室含有可固化形式的细胞培养基。 还描述了可以在所述组织培养插入物上沉积和生长的细胞,其中沉积有细胞的可渗透的组织培养板插入物的组织培养板的传送方法。 还描述了用于运输上述组织培养板的试剂盒和相应的使用方法。