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1. 发明申请

US20070020641A1 Reagentless, reusable, bioelectronic detectors employing aptamers 有权
标题翻译：使用适体的无需重复使用的生物电子检测器
公开(公告)号：US20070020641A1
公开(公告)日：2007-01-25
申请号：US11193318
申请日：2005-07-29
申请人： Alan Heeger , Chunhai Fan , Kevin Plaxco
发明人： Alan Heeger , Chunhai Fan , Kevin Plaxco
IPC分类号： C12Q1/68
CPC分类号： B82Y15/00 , B82Y30/00 , C12Q1/6825 , C12Q2565/607 , C12Q2565/107 , C12Q2525/205 , C12Q2563/113
摘要： A reagentless, reusable bioelectronic DNA, or other oligonucleotide sequence sensor is disclosed. The sensor includes an oligonucleotide (aptamer) probe tagged with a electroactive, redoxable moiety, self-assembled on or near an electrode. This surface-confined oligonucleotide (aptamer) probe structure undergoes hybridization-induced conformational change in the presence of the target which changes the electron-transfer distance between the redoxable moiety and the electrode thereby providing a detectable signal change. In an alternative embodiment, the target can harbor the redoxable moiety.
摘要翻译：公开了一种无试剂，可再利用的生物电子DNA或其他寡核苷酸序列传感器。传感器包括用电活性的可氧化可氧化部分标记的寡核苷酸（适体）探针，其自组装在电极上或附近。该表面限制的寡核苷酸（适体）探针结构在靶的存在下经历杂化诱导的构象变化，其改变可氧化还原部分和电极之间的电子传递距离，从而提供可检测的信号变化。在替代实施方案中，靶可含有可氧化的部分。

2. 发明授权

US08003374B2 Reagentless, reusable, bioelectronic detectors 有权
标题翻译：无源，可重复使用的生物电子探测器
公开(公告)号：US08003374B2
公开(公告)日：2011-08-23
申请号：US11193318
申请日：2005-07-29
申请人： Alan J. Heeger , Chunhai Fan , Kevin Plaxco
发明人： Alan J. Heeger , Chunhai Fan , Kevin Plaxco
IPC分类号： C12M1/34 , C12M3/00 , C12M1/00 , C12Q1/68 , C07H21/04
CPC分类号： B82Y15/00 , B82Y30/00 , C12Q1/6825 , C12Q2565/607 , C12Q2565/107 , C12Q2525/205 , C12Q2563/113
摘要： A reagentless, reusable bioelectronic DNA, or other oligonucleotide sequence sensor is disclosed. The sensor includes an oligonucleotide (aptamer) probe tagged with a electroactive, redoxable moiety, self-assembled on or near an electrode. This surface-confined oligonucleotide (aptamer) probe structure undergoes hybridization-induced conformational change in the presence of the target which changes the electron-transfer distance between the redoxable moiety and the electrode thereby providing a detectable signal change. In an alternative embodiment, the target can harbor the redoxable moiety.
摘要翻译：公开了一种无试剂，可再利用的生物电子DNA或其他寡核苷酸序列传感器。传感器包括用电活性的可氧化可氧化部分标记的寡核苷酸（适体）探针，其自组装在电极上或附近。该表面限制的寡核苷酸（适体）探针结构在靶的存在下经历杂化诱导的构象变化，其改变可氧化还原部分和电极之间的电子传递距离，从而提供可检测的信号变化。在替代实施方案中，靶可含有可氧化的部分。

3. 发明申请

US20050112605A1 Reagentless, reusable, bioelectronic detectors and their use as authentication devices 审中-公开
标题翻译：试剂，可重复使用的生物电子检测器及其作为认证设备的用途
公开(公告)号：US20050112605A1
公开(公告)日：2005-05-26
申请号：US10810333
申请日：2004-03-25
申请人： Alan Heeger , Kevin Plaxco
发明人： Alan Heeger , Kevin Plaxco
IPC分类号： C12M1/34 , C12Q1/68
CPC分类号： B82Y30/00 , B01J2219/00653 , B01J2219/00713 , B01J2219/00722 , B01J2219/00729 , B82Y15/00 , C12Q1/6825 , C12Q2563/113 , C12Q2565/607
摘要： A reagentless, reusable bioelectronic DNA, or other oligonucleotide sequence sensor is disclosed. The sensor includes an oligonucleotide probe tagged with a electroactive, redoxable moiety, self-assembled on or near an electrode. This surface-confined oligonucleotide probe structure undergoes hybridization-induced conformational change in the presence of the target oligonculeotide sequence which change the electron-transfer distance between the redoxable moiety and the electrode thereby providing a detectable signal change. In an alternative embodiment, the target can harbor the redoxable moiety. In a preferred application, the target sequence is associated with an object and its detection is correlated with the authenticity of the object.
摘要翻译：公开了一种无试剂，可再利用的生物电子DNA或其他寡核苷酸序列传感器。传感器包括标记有电活性，可氧化部分的寡核苷酸探针，其自组装在电极上或附近。这种表面限制的寡核苷酸探针结构在靶寡核苷酸序列存在下进行杂交诱导的构象变化，其改变可氧化部分和电极之间的电子传递距离，从而提供可检测的信号变化。在替代实施方案中，靶可含有可氧化的部分。在优选应用中，目标序列与对象相关联，并且其检测与对象的真实性相关。

4. 发明授权

US07803542B2 Signal-on architecture for electronic, oligonucleotide-based detectors 有权
标题翻译：基于寡核苷酸的电子探测器的信号架构
公开(公告)号：US07803542B2
公开(公告)日：2010-09-28
申请号：US11564674
申请日：2006-11-29
申请人： Yi Xiao , Arica Lubin , Kevin Plaxco
发明人： Yi Xiao , Arica Lubin , Kevin Plaxco
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6825 , G01N27/3277 , C12Q2565/133 , C12Q2563/113 , C12Q2525/205 , C12Q2537/163 , C12Q2537/137 , C12Q2565/525 , C12Q2565/549 , C12Q2565/607
摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.
摘要翻译：本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

5. 发明申请

US20070154909A1 SIGNAL-ON ARCHITECTURE FOR ELECTRONIC, OLIGONUCLEOTIDE-BASED DETECTORS 有权
标题翻译：用于电子，基于寡核苷酸的检测器的信号建筑
公开(公告)号：US20070154909A1
公开(公告)日：2007-07-05
申请号：US11564674
申请日：2006-11-29
申请人： Yi Xiao , Arica Lubin , Kevin Plaxco
发明人： Yi Xiao , Arica Lubin , Kevin Plaxco
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6825 , G01N27/3277 , C12Q2565/133 , C12Q2563/113 , C12Q2525/205 , C12Q2537/163 , C12Q2537/137 , C12Q2565/525 , C12Q2565/549 , C12Q2565/607
摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.
摘要翻译：本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

6. 发明申请

US20060286569A1 Method, apparatus, and system for authentication using labels containing nucleotide sequences 审中-公开
标题翻译：使用包含核苷酸序列的标签进行认证的方法，装置和系统
公开(公告)号：US20060286569A1
公开(公告)日：2006-12-21
申请号：US11370531
申请日：2006-03-08
申请人： Yuval Bar-Or , Paul Scheibe , Kevin Plaxco , Arthur Thomas
发明人： Yuval Bar-Or , Paul Scheibe , Kevin Plaxco , Arthur Thomas
IPC分类号： C12Q1/68 , G06F19/00
CPC分类号： C12Q1/68 , C12Q2563/185 , C12Q2525/101
摘要： A method, label, and labeling system for labeling and authenticating an item are presented. At least one of a number of known nucleotide sequences associated with a predetermined amount of information is used as a label to be associated with an item. The label is then read with a reagentless sensor to detect the nucleotide sequence(s). The detected nucleotide sequence(s) is then associated with the appropriate information. The item is authenticated if the sensor detects the expected nucleotide sequence(s). The information in the DNA label may also be passed through a hash function or encrypted to further enhance security. The labels may also incorporate known non-natural nucleic acid analog sequences rather than nucleotide sequences, and a reader that reads known non-natural nucleic acid analog sequences may be employed.
摘要翻译：介绍了一种用于标签和认证项目的方法，标签和标签系统。使用与预定量的信息相关联的多个已知核苷酸序列中的至少一个作为与物品相关联的标签。然后用无试剂传感器读取标签以检测核苷酸序列。检测到的核苷酸序列然后与适当的信息相关联。如果传感器检测到预期的核苷酸序列，则该项目被认证。 DNA标签中的信息也可以通过散列函数或加密进一步增强安全性。标记还可以结合已知的非天然核酸类似物序列而不是核苷酸序列，并且可以使用读取已知非天然核酸类似物序列的阅读器。

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