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1. 发明申请

US20080064061A1 Yeast Promoter 审中-公开
标题翻译：酵母启动子
公开(公告)号：US20080064061A1
公开(公告)日：2008-03-13
申请号：US11659492
申请日：2005-08-02
申请人： Akihiro Meta , Yo Nakahara , Hirofumi Higuchi
发明人： Akihiro Meta , Yo Nakahara , Hirofumi Higuchi
IPC分类号： C12P21/04 , C12N1/00 , C12N15/00 , C12N15/11
CPC分类号： C12N15/81
摘要： The present invention provides a DNA fragment exhibiting a potent promoter activity when used in the yeast. The present invention relates to a DNA fragment possessing a transcription promoter activity of the yeast, said DNA fragment having a whole of the DNA sequence shown by SEQ ID NO: 1 or a portion of said DNA sequence inclusive of its 3′-end, or having a DNA sequence which is hybridizable to a sequence complementary to a whole of the DNA sequence shown by SEQ ID NO: 1 or to a portion of said DNA sequence inclusive of its 3′-end and maintains the transcription promoter activity of said whole or portion of the DNA sequence shown by SEQ ID NO: 1; an expression plasmid containing a recombinant DNA fragment comprising said DNA fragment and a gene coding for a heterologous protein positioned downstream of said DNA sequence; a host cell transformed with said recombinant DNA fragment or said expression plasmid; and a method for preparing a heterologous protein which comprises culturing said host cell and recovering the heterologous protein from the culture. According to the present invention, a potent promoter usable in a heterologous gene expression system with the yeast as a host is provided.
摘要翻译：本发明提供了当在酵母中使用时显示出有效启动子活性的DNA片段。本发明涉及具有酵母的转录启动子活性的DNA片段，所述DNA片段具有SEQ ID NO：1所示的整个DNA序列或所述DNA序列的一部分（包括其3'末端），或具有可与SEQ ID NO：1所示的整个DNA序列互补的序列杂交的DNA序列或包含其3'末端的所述DNA序列的一部分，并保持所述整体的转录启动子活性或 SEQ ID NO：1所示的DNA序列的部分; 含有包含所述DNA片段的重组DNA片段和编码位于所述DNA序列下游的异源蛋白质的基因的表达质粒; 用所述重组DNA片段或所述表达质粒转化的宿主细胞; 以及制备异源蛋白质的方法，其包括培养所述宿主细胞并从培养物中回收异源蛋白质。根据本发明，提供了可用于以酵母作为宿主的异源基因表达系统中的有效启动子。

2. 发明授权

US06191113B1 Peptide for inhibiting growth of smooth muscle cells 失效
标题翻译：抑制平滑肌细胞生长的肽
公开(公告)号：US06191113B1
公开(公告)日：2001-02-20
申请号：US09051986
申请日：1998-04-24
申请人： Yo Nakahara , Saburo Hara , Yuichi Kamikubo , Sumiyo Takemoto , Seiji Miyamoto
发明人： Yo Nakahara , Saburo Hara , Yuichi Kamikubo , Sumiyo Takemoto , Seiji Miyamoto
IPC分类号： A61K3810
CPC分类号： C07K7/06 , A61K38/00 , C07K7/08 , C07K14/47 , C07K14/745 , C07K14/8114 , C07K19/00 , C07K2319/00
摘要： A peptide comprising a peptide (A) of an amino acid sequence being abundant in basic amino acid residues and a peptide (B) of an amino acid sequence comprising at least two consecutive, hydrophobic amino acid residues wherein the peptide (B) is linked to the C-terminal of the peptide (A) directly or via several amino acid residues. The novel peptide of the present invention has the activity to inhibit growth of smooth muscle cells and a pharmaceutical composition comprising the peptide is useful for preventing or treating pathological conditions associated with growth of smooth muscle cells such as arteriosclerosis, restenosis after angioplasty, luminal stenosis after grafting blood vessel and smooth muscle sarcoma.
摘要翻译：包含碱性氨基酸残基丰富的氨基酸序列的肽（A）的肽和包含至少两个连续的疏水性氨基酸残基的氨基酸序列的肽（B），其中肽（B）连接到肽（A）的C-末端直接或通过几个氨基酸残基。本发明的新型肽具有抑制平滑肌细胞生长的活性，包含该肽的药物组合物可用于预防或治疗与平滑肌细胞生长有关的病理状况，如动脉硬化，血管成形术后再狭窄，腔内狭窄后嫁接血管和平滑肌肉瘤。

3. 发明授权

US6060449A Neovascularization inhibitor containing tissue factor pathway inhibitor 失效
标题翻译：包含组织因子途径抑制剂的新血管形成抑制剂
公开(公告)号：US6060449A
公开(公告)日：2000-05-09
申请号：US142479
申请日：1998-09-10
申请人： Tsutomu Hamuro , Yo Nakahara , Sumiyo Takemoto , Seiji Miyamoto
发明人： Tsutomu Hamuro , Yo Nakahara , Sumiyo Takemoto , Seiji Miyamoto
IPC分类号： A61K38/57 , A61P35/00 , A61K38/02 , A61K38/16 , A61K38/55
CPC分类号： A61K38/57 , Y10S514/885 , Y10S514/886 , Y10S514/914
摘要： There is provided an inhibitor of angiogenesis induced by growth of the vascular endothelial cells that comprises Tissue Factor Pathway Inhibitor (TFPI) as an active ingredient. The TFPI-containing preparation of the present invention can effectively inhibit angiogenesis induced by growth of the vascular endothelial cells, and thus, is quite efficacious for preventing and treating the conditions of diseases associated with angiogenesis such as malignant tumors.
摘要翻译： PCT No.PCT / JP97 / 00973 Sec。 371日期：1998年9月10日 102（e）1998年9月10日PCT 1997年3月24日PCT公布。公开号WO97 / 35609 日期1997年10月2日提供了由包含组织因子途径抑制剂（TFPI）作为活性成分的血管内皮细胞生长诱导的血管生成抑制剂。本发明的含TFPI的制剂可有效抑制由血管内皮细胞生长诱导的血管发生，因此对于预防和治疗与血管生成相关疾病如恶性肿瘤的病症相当有效。

4. 发明申请

US20090137001A1 PROCESS FOR PREPARING RECOMBINANT HUMAN THROMBIN WITH CULTURE CELL 审中-公开
标题翻译：用文化细胞制备重组人血红蛋白的方法
公开(公告)号：US20090137001A1
公开(公告)日：2009-05-28
申请号：US12088823
申请日：2006-09-29
申请人： Tatsufumi Onchi , Yo Nakahara , Takayuki Imamura , Toshinobu Nouchi , Hiroshi Nakatake
发明人： Tatsufumi Onchi , Yo Nakahara , Takayuki Imamura , Toshinobu Nouchi , Hiroshi Nakatake
IPC分类号： C12P21/02 , C12N9/74 , C07K14/745 , C12N5/06
CPC分类号： C12N9/6429 , C12Y304/21005
摘要： The present invention provides for a process for preparing a recombinant human thrombin. A process for preparing a recombinant human thrombin which comprises: (1) obtaining a transfectant cell producing a human prothrombin by introducing an expression vector, wherein a gene fragment coding for a human prothrombin gene is incorporated, into an animal cell; (2) purifying a human prothrombin from the culture of the transfectant cell above by an anion exchanger; (3) converting the purified human prothrombin into a human thrombin by subjecting said human prothrombin to the action of ecarin; and (4) purifying the human thrombin from the solution after treatment with ecarin by an affinity method using benzamidine and a cation exchanger, and human thrombin obtained by said process, and a CHO cell that produces human prothrombin.
摘要翻译：本发明提供了制备重组人凝血酶的方法。一种制备重组人凝血酶的方法，其包括：（1）通过将编码人凝血酶原基因的基因片段引入到动物细胞中的表达载体获得产生人凝血酶原的转染细胞; （2）通过阴离子交换剂从上述转染细胞的培养物纯化人凝血酶原; （3）通过使所述人凝血酶原受到ecarin的作用，将纯化的人凝血酶原转化为人凝血酶; 和（4）使用苯甲脒和阳离子交换剂的亲和法，通过所述方法获得的人凝血酶和产生人凝血酶原的CHO细胞，用ecarin处理后，从溶液中纯化人凝血酶。

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