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1. 发明公开

EP1304163A2 Chemical arrays and method for their production 审中-公开
标题翻译： Chemische Arrays und verfahren zu ihrer Herstellung
公开(公告)号：EP1304163A2
公开(公告)日：2003-04-23
申请号：EP02257237.4
申请日：2002-10-18
申请人： Agilent Technologies, Inc.
发明人： Schembri, Carol T. , Lefkowitz, Steven M. , Perbost, Michel G. M. , Kanemoto, Roy H.
IPC分类号： B01J19/00 , G01N21/64
CPC分类号： G01N21/6452 , B01J19/0046 , B01J2219/00274 , B01J2219/00378 , B01J2219/00497 , B01J2219/00518 , B01J2219/00527 , B01J2219/00533 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B82Y30/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B50/14 , C40B60/14 , C40B70/00 , G01N21/6428 , Y10T428/24868
摘要： An array assembly and a method of fabricating such an assembly. The array assembly may include a plastic base layer (14a), a glass layer (14d) forward of the base layer, and an array (12) of polymers having a pattern of features on a front surface (11a) of the glass layer. A method of reading an array is also provided in which the array has a plastic base layer (14a), a glass layer (14d) forward of the base layer, a reflective layer (14c) intermediate the base and glass layers, and an array on a front surface of the glass layer. The method may include illuminating features of the array and detecting any resulting fluorescence.
摘要翻译：阵列组件和制造这种组件的方法。阵列组件可以包括塑料基层（14a），基层前面的玻璃层（14d）和在玻璃层的前表面（11a）上具有特征图案的聚合物阵列（12）。还提供了一种读取阵列的方法，其中阵列具有塑料基底层（14a），基底层前方的玻璃层（14d），基底和玻璃层之间的反射层（14c）和阵列在玻璃层的前表面上。该方法可以包括照射阵列的特征并检测任何所得到的荧光。

2. 发明公开

EP1186671A3 Method for hybridization of arrays on siliceous surfaces 审中-公开
标题翻译：用于在硅表面杂交阵列方法
公开(公告)号：EP1186671A3
公开(公告)日：2003-12-17
申请号：EP01121185.1
申请日：2001-09-04
申请人： Agilent Technologies, Inc. (a Delaware corporation)
发明人： Shannon, Karen W. , Lefkowitz, Steven M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2527/119 , C12Q2527/101
摘要： A method (100, 200) of hybridizing arrays of nucleic acids an surface-derivatized siliceous substrates with other nucleic acid materials provides an envelope of conditions to produce sensitive, selective detection of nucleic acid targets, while preserving the intactness of the derivatized surface of the array. The envelope of hybridization conditions includes a hybridization solution having a pH between pH 5.5 and 6.7 and a high hybridization or incubation temperature between 55°C and 70°C. In one embodiment (100), the hybridization solution is maintained (102) at the pH between pH 5.5 and 6.7 and the array is incubated (104) with a nucleic acid material in the pH-maintained hybridization solution at the hybridization temperature of between 55°C and 70°C. The pH of the hybridization solution is maintained (102) with a buffer having a useful buffering capacity between pH 5.5 and 6.7. In another embodiment (200), a nucleic acid material is combined (202) with the hybridization solution at the pH between pH 5.5 to 6.7 containing a buffer and a monovalent cation and the combined solution is incubated (204) with the array at the hybridization temperature of between 55°C and 70°C so as to hybridize the nucleic acid material. Typical hybridization times can range from less than 2 hours to more than 48 hours. The present method is particularly useful for hybridization assays an silylated-siliceous substrates where the incubation time is much greater than about 6 hours at the high hybridization temperature. The envelope of hybridization conditions provide optimized assay performance while maintaining the integrity of the derivatized surface of the siliceous substrate. A kit comprises a microarray having a siliceous substrate with a derivatized surface and an oligonucleotide populated an the surface for hybridizing a another oligonucleotide material. The kit further includes instructions for using the microarray in accordance with the method (100, 200) of the present invention.

3. 发明公开

EP1304572A1 Chemical arrays 审中-公开
标题翻译：化学数组
公开(公告)号：EP1304572A1
公开(公告)日：2003-04-23
申请号：EP02257235.8
申请日：2002-10-18
申请人： Agilent Technologies, Inc.
发明人： Perbost, Michael G.M. , Lefkowitz, Steven M. , Kanemoto, Roy H. , Schembri, Carol T.
IPC分类号： G01N33/543 , B01J19/00
CPC分类号： B01J19/0046 , Y10T436/110833 , Y10T436/143333 , Y10T436/25
摘要： A method and apparatus for using arrays (12) of polymers each having a pattern of features over a corresponding array region on a surface (11a) of a flexible elongated web (10). In this method each array region may be exposed to a corresponding continuous volume of a sample fluid. A method and apparatus are also provided for reading arrays (12) disposed along a surface of a flexible elongated web (10). In one such method the web (10) with the arrays thereon may be transported in a lengthwise direction past a reading location (610) at which a characteristic of the features (16) is read, while restraining the web (10) on both surfaces (11a, 11b) on either side of the reading location (610) to assist in maintaining the reading location (610) flat.
摘要翻译：一种使用聚合物阵列（12）的方法和装置，每个聚合物在柔性细长网（10）的表面（11a）上的相应阵列区域上具有特征图案。在该方法中，每个阵列区域可以暴露于相应的连续体积的样品流体。还提供了一种用于读取沿柔性细长网（10）的表面设置的阵列（12）的方法和装置。在一种这样的方法中，其上具有阵列的腹板（10）可以沿长度方向传送通过读取位置（610），在该位置读取特征（16）的特征，同时在两个表面上限制腹板（10）（610）的任一侧上的所述读取位置（610a）的平面（11a，11b）。

4. 发明公开

EP1304162A3 Chemical arrays and method for their production 审中-公开
标题翻译：化学阵列，以及它们的制备方法
公开(公告)号：EP1304162A3
公开(公告)日：2004-04-21
申请号：EP02257236.6
申请日：2002-10-18
申请人： Agilent Technologies, Inc.
发明人： Lefkowitz, Steven M. , Kanemoto, Roy H. , Schembri, Carol T. , Perbost, Michel G.M.
IPC分类号： B01J19/00 , G06F19/00 , C12Q1/68
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00274 , B01J2219/00378 , B01J2219/00497 , B01J2219/00518 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00695 , C40B50/14 , C40B60/14 , C40B70/00 , G01N35/00009 , G01N2035/00158
摘要： Methods, apparatus, and computer program products to form arrays (12) of polymers each having a pattern of features on a surface (11a) of a flexible elongated web (10). In a method polymers or their precursor units are applied at an application station to the surface. Multiple features (16) are covered at a reagent station (384) with a continuous volume of reagent which chemically reacts with precursors or the web (10). The flexible elongated web (10) is driven in a lengthwise direction through the application station. This sequence may be repeated as needed to form the arrays along the web. Also provided is a method preparing a surface of a flexible elongated web (10) to receive a biopolymer array (12).

5. 发明公开

EP1304162A2 Chemical arrays and method for their production 审中-公开
标题翻译： Chemische Arrays und Verfahren zu ihrer Herstellung
公开(公告)号：EP1304162A2
公开(公告)日：2003-04-23
申请号：EP02257236.6
申请日：2002-10-18
申请人： Agilent Technologies, Inc.
发明人： Lefkowitz, Steven M. , Kanemoto, Roy H. , Schembri, Carol T. , Perbost, Michel G.M.
IPC分类号： B01J19/00 , G06F19/00 , C12Q1/68
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00274 , B01J2219/00378 , B01J2219/00497 , B01J2219/00518 , B01J2219/00527 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00695 , C40B50/14 , C40B60/14 , C40B70/00 , G01N35/00009 , G01N2035/00158
摘要： Methods, apparatus, and computer program products to form arrays (12) of polymers each having a pattern of features on a surface (11a) of a flexible elongated web (10). In a method polymers or their precursor units are applied at an application station to the surface. Multiple features (16) are covered at a reagent station (384) with a continuous volume of reagent which chemically reacts with precursors or the web (10). The flexible elongated web (10) is driven in a lengthwise direction through the application station. This sequence may be repeated as needed to form the arrays along the web. Also provided is a method preparing a surface of a flexible elongated web (10) to receive a biopolymer array (12).
摘要翻译：方法，装置和计算机程序产品以形成聚合物阵列（12），每个聚合物在柔性细长网（10）的表面（11a）上具有特征图案。在一种方法中，将聚合物或其前体单元在施用站施加到表面。多个特征（16）用试剂站（384）覆盖有连续体积的试剂，其与前体或网（10）发生化学反应。柔性细长网（10）沿长度方向驱动通过施工台。可以根据需要重复该序列以沿着网形成阵列。还提供了制备柔性细长网（10）的表面以接收生物聚合物阵列（12）的方法。

6. 发明公开

EP1288664A1 Methods for generating ligand arrays 有权
标题翻译： Verfahren zur Herstellung von Liganden-Arrays
公开(公告)号：EP1288664A1
公开(公告)日：2003-03-05
申请号：EP02256004.9
申请日：2002-08-29
申请人： Agilent Technologies, Inc.
发明人： Lefkowitz, Steven M. , Kim, Namyong , Holcomb, Nelson R. , Hargreaves, John S. , Dellinger, Geraldine F. , Dellinger, Douglas J.
IPC分类号： G01N33/543 , B01J19/00
CPC分类号： B82Y30/00 , B01J2219/00364 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54353 , G01N33/551
摘要： Methods of producing ligand arrays, e.g., polypeptide and nucleic acid arrays, as well as the arrays produced thereby, methods for use of the arrays and kits that include the same, are provided. In the subject methods, a substrate having a surface displaying olefinic functional groups, e.g., olefin groups having a single site of unsaturation, are modified such that the olefinic functional groups are converted to ligand reactive functional groups. The resultant substrate is then contacted with ligands, e.g., via deposition of each different ligand onto a different region of the surface, resulting in covalent attachment of the contacted ligand to the surface via reaction with the ligand reactive functional groups. Ligand arrays produced via the subject methods demonstrate a number of desirable properties, e.g., nucleic acid arrays produced by the subject methods provide high signal intensity with low background in nucleic acid hybridization assays, etc.
摘要翻译：提供了制备配体阵列的方法，例如多肽和核酸阵列，以及由此产生的阵列，使用阵列的方法和包括其的试剂盒。在本发明方法中，修饰具有显示烯属官能团的表面的基材，例如具有单一不饱和位点的烯烃基团，使得烯属官能团转化为配体反应性官能团。然后将所得底物与配体接触，例如通过将各不同配体沉积在表面的不同区域上，从而通过与配体反应性官能团的反应将接触的配体共价连接到表面。通过本发明方法制备的配体阵列证明了许多所需的性质，例如由本发明方法产生的核酸阵列在核酸杂交测定等中提供具有低背景的高信号强度。

7. 发明授权

EP1288664B1 Methods for generating ligand arrays 有权
标题翻译：一种用于配体阵列的制备过程
公开(公告)号：EP1288664B1
公开(公告)日：2007-03-07
申请号：EP02256004.9
申请日：2002-08-29
申请人： Agilent Technologies, Inc.
发明人： Lefkowitz, Steven M. , Kim, Namyong , Holcomb, Nelson R. , Hargreaves, John S. , Dellinger, Geraldine F. , Dellinger, Douglas J.
IPC分类号： G01N33/543 , B01J19/00
CPC分类号： B82Y30/00 , B01J2219/00364 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/00605 , B01J2219/00608 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722 , B01J2219/00725 , C40B40/06 , C40B40/10 , C40B60/14 , G01N33/54353 , G01N33/551

8. 发明公开

EP1304163A3 Chemical arrays and method for their production 审中-公开
标题翻译：化学阵列和它们的制备方法
公开(公告)号：EP1304163A3
公开(公告)日：2004-04-21
申请号：EP02257237.4
申请日：2002-10-18
申请人： Agilent Technologies, Inc.
发明人： Schembri, Carol T. , Lefkowitz, Steven M. , Perbost, Michel G. M. , Kanemoto, Roy H.
IPC分类号： B01J19/00
CPC分类号： G01N21/6452 , B01J19/0046 , B01J2219/00274 , B01J2219/00378 , B01J2219/00497 , B01J2219/00518 , B01J2219/00527 , B01J2219/00533 , B01J2219/00547 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00617 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00722 , B01J2219/00725 , B01J2219/00731 , B82Y30/00 , C40B40/06 , C40B40/10 , C40B40/12 , C40B50/14 , C40B60/14 , C40B70/00 , G01N21/6428 , Y10T428/24868
摘要： An array assembly and a method of fabricating such an assembly. The array assembly may include a plastic base layer (14a), a glass layer (14d) forward of the base layer, and an array (12) of polymers having a pattern of features on a front surface (11a) of the glass layer. A method of reading an array is also provided in which the array has a plastic base layer (14a), a glass layer (14d) forward of the base layer, a reflective layer (14c) intermediate the base and glass layers, and an array on a front surface of the glass layer. The method may include illuminating features of the array and detecting any resulting fluorescence.

9. 发明公开

EP1302548A1 Buffer composition for hybridization of microarrays 审中-公开
标题翻译：用于杂交到微阵列缓冲液组成
公开(公告)号：EP1302548A1
公开(公告)日：2003-04-16
申请号：EP02254696.4
申请日：2002-07-04
申请人： Agilent Technologies, Inc.
发明人： Holcomb, Nelson R. , Collins, Patrick J. , Shannon, Karen W. , Lefkowitz, Steven M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： A buffer composition, method and kit for hybridizing microarrays of nucleic acids bound to an adsorbed polymer surface of a siliceous substrate provide an envelope of conditions to hybridize nucleic acid targets, while preserving the intactness of the adsorbed polymer surface of the array. The buffer composition comprises a non-chelating buffering agent, a pH between pH 6.4 and 7.5, a monovalent cation having a monovalent cation concentration that ranges from 0.01 M to 2.0 M, and optionally, relatively smaller concentrations of a chelating agent and an ionic surfactant. The total cation concentration of the buffer composition ranges from about 0.02 M to about 2.0 M. The method comprises incubating the targets with a hybridization solution comprising the buffer composition and the siliceous substrate at a temperature between 55°C and 70°C.

10. 发明公开

EP1186671A2 Method for hybridization of arrays on siliceous surfaces 审中-公开
标题翻译： Methode zur Hybridisierung von Arrays aufSilikonoberflächen
公开(公告)号：EP1186671A2
公开(公告)日：2002-03-13
申请号：EP01121185.1
申请日：2001-09-04
申请人： Agilent Technologies Inc. (a Delaware Corporation)
发明人： Shannon, Karen W. , Lefkowitz, Steven M.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12Q2527/119 , C12Q2527/101
摘要： A method (100, 200) of hybridizing arrays of nucleic acids an surface-derivatized siliceous substrates with other nucleic acid materials provides an envelope of conditions to produce sensitive, selective detection of nucleic acid targets, while preserving the intactness of the derivatized surface of the array. The envelope of hybridization conditions includes a hybridization solution having a pH between pH 5.5 and 6.7 and a high hybridization or incubation temperature between 55°C and 70°C. In one embodiment (100), the hybridization solution is maintained (102) at the pH between pH 5.5 and 6.7 and the array is incubated (104) with a nucleic acid material in the pH-maintained hybridization solution at the hybridization temperature of between 55°C and 70°C. The pH of the hybridization solution is maintained (102) with a buffer having a useful buffering capacity between pH 5.5 and 6.7. In another embodiment (200), a nucleic acid material is combined (202) with the hybridization solution at the pH between pH 5.5 to 6.7 containing a buffer and a monovalent cation and the combined solution is incubated (204) with the array at the hybridization temperature of between 55°C and 70°C so as to hybridize the nucleic acid material. Typical hybridization times can range from less than 2 hours to more than 48 hours. The present method is particularly useful for hybridization assays an silylated-siliceous substrates where the incubation time is much greater than about 6 hours at the high hybridization temperature. The envelope of hybridization conditions provide optimized assay performance while maintaining the integrity of the derivatized surface of the siliceous substrate. A kit comprises a microarray having a siliceous substrate with a derivatized surface and an oligonucleotide populated an the surface for hybridizing a another oligonucleotide material. The kit further includes instructions for using the microarray in accordance with the method (100, 200) of the present invention.
摘要翻译：将表面衍生的硅质底物与其他核酸材料的核酸阵列杂交的方法（100,200）提供条件的包络，以产生对核酸靶的敏感，选择性检测，同时保持衍生化表面的完整性阵列。杂交条件的包络包括pH在5.5和6.7之间的pH值和55℃至70℃之间的高杂交或孵育温度的杂交溶液。在一个实施方案（100）中，将杂交溶液维持在（102）在pH 5.5和6.7之间的pH值，并且在55℃至70℃的杂交温度下，将该阵列与含有pH值的杂交溶液中的核酸材料一起孵育（104）。保持杂交溶液的pH（102 ）与具有在5.5和6.7之间的有用缓冲能力的缓冲液。在另一个实施方案（200）中，将核酸材料与杂交溶液在含有缓冲液和一价阳离子的pH5.5至6.7之间的pH下结合（202），并将合并的溶液与杂交（204）与阵列孵育温度在55℃至70℃之间，以使核酸材料杂交。典型的杂交时间可以从小于2小时到超过48小时。本方法对于硅烷化硅质底物的杂交测定特别有用，其中在高杂交温度下孵育时间远大于约6小时。杂交条件的包络提供优化的测定性能，同时保持硅质底物的衍生化表面的完整性。试剂盒包括具有带有衍生化表面的硅质底物的微阵列和用于杂交另一种寡核苷酸材料的表面的寡核苷酸。该试剂盒还包括根据本发明的方法（100,200）使用微阵列的说明书。

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