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    • 4. 发明申请
    • Displacement sandwich immuno-PCR
    • 位移夹心免疫PCR
    • US20050026161A1
    • 2005-02-03
    • US10701347
    • 2003-11-03
    • Edward JablonskiDavid DriverThomas Adams
    • Edward JablonskiDavid DriverThomas Adams
    • C12N20060101C12Q1/68G01N33/53G01N33/536G01N33/537G01N33/543
    • G01N33/54306C12Q1/6804G01N2458/10C12Q2537/1373
    • The present invention is directed to labeling antibodies with DNA in a convenient kit format for the purpose of producing and utilizing nucleic acid detection immunoassays. The process comprises treating a reporter antibody with a chemically activated strand of DNA, which serves as a template for any DNA amplification system. The reporter antibody-DNA conjugate is used to form a complex with corresponding antigen. The complex can be detected by the production of multiple copies of the DNA template. The kit may also provide an activated DNA sequence to label a capture antibody. The DNA conjugated to the capture antibody is modified to contain a high affinity binding ligand for the purpose of coating a solid support. The coated solid support functions to capture the immune complex containing reporter antibody in a sandwich assay format. The DNA sequence of the capture antibody may also have properties providing for the detachment of the complex from the solid support.
    • 本发明涉及以方便的试剂盒形式用DNA标记抗体,用于生产和利用核酸检测免疫测定。 该方法包括用化学活化的DNA链来处理报道抗体,其用作任何DNA扩增系统的模板。 使用报告抗体-DNA缀合物与相应的抗原形成复合物。 可以通过生产DNA模板的多个拷贝来检测该复合物。 试剂盒还可以提供活化的DNA序列以标记捕获抗体。 与捕获抗体缀合的DNA被修饰为含有高亲和力结合配体,用于涂覆固体支持物。 包被的固体支持物用于以夹心测定形式捕获含有报道抗体的免疫复合物。 捕获抗体的DNA序列也可以具有提供复合物与固体支持物分离的性质。