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    • 2. 发明申请
    • POINT-OF-CARE FLUORESCENT IMMUNOASSAY FOR IDENTIFYING BIOMARKERS IN PATIENT BIOFLUID SAMPLES
    • 在患者生物流体样本中识别生物标志物的点保护荧光免疫
    • WO2016195918A1
    • 2016-12-08
    • PCT/US2016/031203
    • 2016-05-06
    • ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
    • KATCHMAN, BenjaminANDERSON, KarenSMITH, JosephBLAIN CHRISTEN, Jennifer
    • G01N33/53G01N33/569C12Q1/70
    • G01N33/54373B01L3/502715G01N21/6428G01N33/54386G01N33/58G01N33/582G01N33/6857G01N2333/025
    • Systems and methods for low-cost point-of-care immunoassay are provided. The system comprises an emitter, two optical interference filters, a microscope slide, a photodiode detector, a circuit, and a measuring unit. The detector is placed upon the second filter, the slide, the first filter, and then the emitter. The emitter comprises non-organic light emitting diodes (LEDs) or organic light emitting diodes (OLEDs) that emits light of a first color. The slide is spotted with biofluid from a patient. Biomarkers in the biofluid is bound with immobilized fluorophores that emit light of a second color when stimulated by the light of the first color. The first and second filters band-pass the light of the first and second colors, respectively. The detector detects light of the second color. The current outputted from the detector is converted into a relatively-large output voltage by a circuit. A measuring unit measures the ramp time of the output voltage. The ramp time is to be used to determine the concentration of the fluorophores, which in turn is related to the concentration of the biomarkers.
    • 提供了低成本免疫检测方法。 该系统包括发射器,两个光学干涉滤光器,显微镜载玻片,光电二极管检测器,电路和测量单元。 检测器放置在第二个过滤器,滑块,第一个过滤器,然后是发射器。 发射器包括发射第一种颜色的光的非有机发光二极管(LED)或有机发光二极管(OLED)。 从患者的生物流中发现载玻片。 当被第一种颜色的光刺激时,生物流体中的生物标志物与固定的发出第二种颜色的荧光团结合。 第一和第二滤光器分别对第一和第二颜色的光进行带通。 检测器检测第二种颜色的光。 从检测器输出的电流被电路转换成相对较大的输出电压。 测量单元测量输出电压的斜坡时间。 斜坡时间用于确定荧光团的浓度,这又与生物标志物的浓度有关。