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1. 发明申请

WO2009015290A1 SYSTEMS AND METHODS FOR ISOLATING NUCLEIC ACIDS 审中-公开
标题翻译：用于分离核酸的系统和方法
公开(公告)号：WO2009015290A1
公开(公告)日：2009-01-29
申请号：PCT/US2008/071071
申请日：2008-07-24
申请人： APPLIED BIOSYSTEMS INC. , VANN, Charles , BREVNOV, Maxim , RUFF, David , LIVAK, Kenneth
发明人： VANN, Charles , BREVNOV, Maxim , RUFF, David , LIVAK, Kenneth
IPC分类号： C12N15/10 , G01N27/447 , B01D57/02
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.
摘要翻译：用于从样品收集目标核酸的系统可以包括至少一个样品室，其被配置为接收含有靶核酸和其它材料的样品，至少一个收集室，其相对于至少一个样品室可拆卸地安装并且被配置成收集目标与其他材料分离的核酸，相对于至少一个样品室可拆卸地安装的过滤器，并且被配置为当所述至少一个收集室相对于所述至少一个收集室安装时，被设置在所述至少一个样品室和所述至少一个收集室之间所述至少一个样品室。该系统可以进一步包括一对电极，其被配置为产生足以使所述至少一个样品室中的靶核酸经由电泳从所述至少一个样品室通过所述过滤器迁移到所述至少一个收集室中的电场，其中所述过滤器可以被配置为允许靶核酸的通过并阻止尺寸大于所述靶核酸的材料的通过。

2. 发明公开

EP2185705A1 SYSTEMS AND METHODS FOR ISOLATING NUCLEIC ACIDS 审中-公开
标题翻译：系统和方法分离核酸
公开(公告)号：EP2185705A1
公开(公告)日：2010-05-19
申请号：EP08782338.1
申请日：2008-07-24
申请人： Applied Biosystems Inc.
发明人： VANN, Charles , BREVNOV, Maxim , RUFF, David , LIVAK, Kenneth
IPC分类号： C12N15/10 , G01N27/447 , B01D57/02
CPC分类号： C12N15/101 , G01N27/44782
摘要： A system for collecting target nucleic acids from a sample can include at least one sample chamber configured to receive a sample containing target nucleic acids and other material, at least one collection chamber removably mountable relative to the at least one sample chamber and configured to collect target nucleic acids separated from the other material, a filter removably mountable relative to the at least one sample chamber and configured to be disposed between the at least one sample chamber and the at least one collection chamber when the at least one collection chamber is mounted relative to the at least one sample chamber. The system may further include a pair of electrodes configured to generate an electric field sufficient to cause target nucleic acids in the at least one sample chamber to migrate via electrophoresis from the at least one sample chamber through the filter into the at least one collection chamber, wherein the filter may be configured to permit passage of target nucleic acids and to block passage of material of a size larger than the target nucleic acids.

3. 发明申请

WO2006102401A2 NORMALIZATION OF GENE EXPRESSION DATA 审中-公开
标题翻译：基因表达数据的正规化
公开(公告)号：WO2006102401A2
公开(公告)日：2006-09-28
申请号：PCT/US2006/010383
申请日：2006-03-22
申请人： APPLERA CORPORATION , SHANNON, Mark , OLDHAM, Mark F. , RUFF, David
发明人： SHANNON, Mark , OLDHAM, Mark F. , RUFF, David
IPC分类号： C12Q1/68 , G06F19/00 , G01N33/48 , G01N33/50 , C12P19/34
CPC分类号： G06F19/20 , C12Q1/686 , C40B30/04
摘要： A method for determining bias across two domains comprising gene expression data. The method can comprise (a) providing a first domain and a second domain; (b) obtaining information indicative of a bias within the first domain; (c) obtaining information indicative of a bias within the second domain; and (d) using the information indicative of the bias within the first domain and the information indicative of the bias within the second domain to produce an indication of bias across the two domains.
摘要翻译：用于确定包含基因表达数据的两个结构域之间的偏差的方法。该方法可以包括（a）提供第一域和第二域; （b）获得指示第一域内偏倚的信息; （c）获得指示第二域内偏倚的信息; 以及（d）使用指示第一域内的偏差的信息和指示第二域内的偏差的信息来产生跨越两个域的偏差的指示。

4. 发明申请

WO2012099896A2 WORKFLOW FOR DETECTION OF LIGANDS USING NUCLEIC ACIDS 审中-公开
标题翻译：使用核酸检测配体的工作流程
公开(公告)号：WO2012099896A2
公开(公告)日：2012-07-26
申请号：PCT/US2012/021585
申请日：2012-01-17
申请人： LIFE TECHNOLOGIES CORPORATION , CHEN, Shiaw-Min , SWARTZMAN, Elana , RUFF, David , SHANNON, Mark , LU, Julia , HENDRICKS, Stephen
发明人： CHEN, Shiaw-Min , SWARTZMAN, Elana , RUFF, David , SHANNON, Mark , LU, Julia , HENDRICKS, Stephen
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6855 , C12Q1/6804 , C12Q2537/101 , C12Q2561/101 , C12Q2561/125
摘要： This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.
摘要翻译：本申请涉及用于连接与靶核酸具有互补性的寡核苷酸并扩增连接的寡核苷酸的方法，其中连接和扩增发生在相同的反应混合物中。

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